LOCUS       RR204835                8949 bp    DNA     circular  SYN 
DEFINITION  Kdr (Myc-DDK-tagged ORF) - Rat kinase insert domain protein receptor (Kdr), (10 ug)
ACCESSION   NM_013062
VERSION     NM_013062.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5106..5197 
                     /label="Myc-DDK"  
     misc_feature    5227..5812 
                     /label="PolyA signal"  
     misc_feature    5284..5303 
                     /label="Restriction Enzyme"  
     misc_feature    6088..6760 
                     /label="ColE1"  
     misc_feature    7190..7984 
                     /label="Kan/Neo"  
     misc_feature    8019..8321 
                     /label="SV40 ori"  
     misc_feature    8407..8884 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5088..5094 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5088 
                     /label="Kdr"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGAGCAGG GCGCTGCTAG CTGTCGCTCT GTGGTTCTGC GTGGAGACCC 
     1081 GAGCCGCCTC TGTGGGTTTG CCTGGCGATT CCCTCCATCC ACCCAAGCTC AGCACACAAA 
     1141 AAGACATACT TACAATTTTG GCAAATACAA CCCTTCAGAT TACTTGCAGG GGACAGAGGG 
     1201 ACCTGGATTG GCTTTGGCCC AACACTCCGC GTGACTCTGA GGAAAGGGTG TTGGTGACTG 
     1261 AGTGTGGCGA CAGTATCTTC TGCAAGACAC TCACAGTTCC CAGAGTGGTT GGAAATGATA 
     1321 CTGGAGCCTA CAAGTGCTTC TATCGGGACA CCGATGTCTC CTCCATCGTT TATGTCTATG 
     1381 TTCAAGATCA CAGGTCACCA TTCATCGCCT CTGTCAGTGA CGAGCATGGC ATCGTGTACA 
     1441 TCACTGAGAA CAAGAACAAA ACTGTGGTGA TCCCATGCCG AGGGTCGATT TCAAACCTCA 
     1501 ACGTGTCACT TTGTGCTAGG TATCCAGAAA AGAGATTTGT TCCGGATGGA AACAGAATTT 
     1561 CCTGGGACAG CGAGAAAGGC TTTACTATCC CCAGTTACAT GATCAGCTAT GCCGGCATGG 
     1621 TCTTCTGTGA GGCAAAGATT AATGATGAAA CGTATCAGTC TATCATGTAC ATAGTTCTGG 
     1681 TTGTAGGATA TAGGATTTAT GATGTGGTCC TGAGCCCCCC TCATGAAATT GAGCTATCTG 
     1741 CCGGAGAAAA GCTTGTCTTA AATTGTACAG CAAGAACAGA GCTCAACGTG GGGCTTGATT 
     1801 TCAGCTGGCA ATTCCCGTCC TCAAAGCATC AGCATAAGAA GATTGTAAAC CGGGATGTGA 
     1861 AATCCCTTCC TGGGACTGTG GCAAAGATGT TTTTGAGCAC CTTGACCATA GACAGTGTGA 
     1921 CCAAGAGTGA CCAAGGAGAA TACACCTGCA CAGCGTACAG TGGACTGATG ACCAAGAAAA 
     1981 ATAAAACATT TGTCCGAGTT CATACAAAAC CTTTTATTGC TTTTGGTAGC GGGATGAAAT 
     2041 CTTTGGTGGA AGCCACTGTG GGCAGCCAAG TCCGAATCCC TGTGAAGTAT CTCAGTTACC 
     2101 CAGCTCCTGA TATCAAATGG TACAGAAATG GACGACCCAT TGAGTCCAAT TACACAATGA 
     2161 TCGTTGGTGA TGAACTCACC ATCATGGAAG TGAGTGAAAG AGATGCGGGA AACTACACGG 
     2221 TCATCCTCAC CAATCCCATT TCAATGGAGA AACAGAGCCA CATGGTCTCT CTGGTTGTGA 
     2281 ATGTTCCACC CCAGATCGGT GAGAAAGCCT TGATCTCTCC TATGGATTCC TACCAGTATG 
     2341 GCACCATGCA GACGCTGACA TGCACAGTCT ATGCCAACCC TCCCCTGCAC CACATCCAAT 
     2401 GGTACTGGCA GCTAGAAGAA GCATGCTCCT ACAGGCCCAG CCAAACAAAC CCATATACTT 
     2461 GTAAAGAATG GAGACACGTG AAGGATTTCC AGGGGGGAAA TAAGATCGAA GTCACCAAAA 
     2521 ACCAATATGC CCTAATTGAA GGAAAAAACA AAACTGTAAG TACTCTGGTC ATCCAGGCTG 
     2581 CCTACGTGTC CGCATTATAC AAATGTGAAG CCATCAACAA AGCAGGACGA GGAGAGAGGG 
     2641 TCATCTCCTT CCATGTGATC AGGGGTCCTG AAATTACTGT CCAGCCTGCT ACCCAGCCAA 
     2701 CCGAGCGGGA GAGTATGTCT TTATTGTGCA CTGCAGATAG AAACACGTTT GAGAACCTCA 
     2761 CGTGGTACAA GCTTGGCTCA CAGGCAACAT CGGTCCACAT GGGCGAATCA CTCACACCAG 
     2821 TTTGCAAGAA CTTGGACGCT CTTTGGAAAC TGAATGGCAC CGTGTTTTCT AACAGCACAA 
     2881 ACGACATCTT GATTGTGGCA TTCCAGAATG CCTCCCTGCA GGACCAAGGC AACTATGTCT 
     2941 GCTCTGCTCA AGACAAGAAG ACCAAGAAAA GACATTGCCT AGTCAAGCAG CTCGTCATCC 
     3001 TAGAGCGCAT GGCACCCATG ATCACTGGAA ATCTGGAGAA TCAGACAACA ACCATTGGTG 
     3061 AGACCATCGA AGTTGTTTGT CCAACATCTG GAAACCCTAC CCCCCTCATT ACATGGTTCA 
     3121 AAGACAATGA GACCCTTGTA GAAGATTCAG GCATTGTACT AAAAGACGGG AACCGGAACC 
     3181 TAACTATCCG AAGGGTGAGG AAGGAAGACG GGGGCCTCTA CACCTGCCAG GCCTGCAATG 
     3241 TCCTTGGCTG TGCAAGAGCA GAGACACTCT TCATAATAGA AGGCGTCCAG GAAAAGACCA 
     3301 ACTTGGAAGT CATTATCCTC GTCGGCACTG CAGTGATTGC CATGTTCTTC TGGCTCCTTC 
     3361 TTGTCATTCT CGTACGGACC GTTAAGCGGG CCAATGAAGG GGAACTGAAG ACAGGCTACT 
     3421 TGTCCATTGT CATGGATCCA GATGAACTGC CCTTGGATGA GCGCTGTGAA CGCTTGCCTT 
     3481 ATGATGCCAG CAAGTGGGAG TTCCCCAGGG ACCGGCTGAA ACTAGGAAAA CCTCTTGGCC 
     3541 GTGGTGCCTT TGGCCAAGTG ATTGAGGCAG ATGCCTTTGG AATCGACAAG ACAGCGACTT 
     3601 GCAAAACAGT GGCTGTCAAG ATGTTGAAAG AGGGAGCAAC ACACAGCGAG CACCGAGCCC 
     3661 TCATGTCCGA ACTCAAGATC CTCATCCACA TTGGCCACCA TCTCAATGTG GTGAACCTGC 
     3721 TGGGTGCCTG CACGAAGCCC GGAGGGCCTC TCATGGTGAT TGTAGAATTC TGCAAGTTTG 
     3781 GAAACCTATC AACTTACTTA CGGGGCAAGA GAAATGAATT CGTGCCCTAT AAGAGCAAAG 
     3841 GGGCACGCTT CCGCTCTGGG AAAGACTATG TTGGGGAGCT CTCCGTAGAC CTGAAGCGGC 
     3901 GCTTGGACAG CATCACCAGC AGTCAGAGCT CTGCCAGCTC AGGTTTTGTG GAGGAGAAAT 
     3961 CCCTCAGTGA CGTAGAGGAA GAAGAAGCTT CTGAAGAACT GTACAAGGAC TTCCTGACCT 
     4021 TGGAGCATCT CATCTGTTAC AGCTTCCAAG TGGCTAAGGG CATGGAGTTC TTGGCATCAA 
     4081 GGAAGTGTAT CCACAGGGAC CTGGCAGCAC GAAACATTCT CCTATCGGAG AAGAACGTGG 
     4141 TTAAAATCTG TGACTTTGGC TTGGCCCGGG ACATTTATAA AGACCCAGAT TACGTCAGAA 
     4201 AAGGAGATCC CCGACTCCCT TTGAAGTGGA TGGCTCCGGA AACAATTTTT GACAGAATAT 
     4261 ACACAATTCA GAGTGGTGTG TGGTCTTTTG GTGTTTTGCT CTGGGAAATA TTTTCCTTAG 
     4321 GTGCTTCCCC ATATCCTGGG GTCAAGATTG ATGAAAAATT TTGTAGGAGA TTGAAAGAAG 
     4381 GAACGAGAAT GCGGGCTCCT GACTACACCA CCCCAGAAAT GTACCAAACC ATGCTGGATT 
     4441 GCTGGCATGA GGACCCCAAC CAGAGACCCG CGTTTTCAGA GTTGGTGGAG CACTTGGGAA 
     4501 ATCTCCTGCA AGCAAATGCT CAGCAGGATG GCAAAGACTA TATTGTTCTT CCAATGTCAG 
     4561 AGACACTGAG CATGGAAGAG GATTCTGGAC TCTCCCTGCC TACCTCACCT GTTTCCTGTA 
     4621 TGGAGGAAGA GGAAGTGTGC GACCCCAAAT TCCATTATGA CAACACAGCA GGAATCAGTC 
     4681 ATTATCTGCA GAACAGCAAG CGAAAAAGCC GGCCAGTGAG TGTAAAAACA TTTGAAGATA 
     4741 TCCCTTTGGA GGAACCAGAA GTAAAAGTGA TTCCAGATGA CAGCCAGACA GACAGTGGGA 
     4801 TGGTCCTTGC CTCAGAAGAG CTGAAAACTC TGGAAGACAG GAACAAATTA TCTCCATCTT 
     4861 TTGGTGGGAT GATGCCCAGT AAAAGCAGGG AGTCTGTGGC CTCGGAAGGC TCCAACCAGA 
     4921 CCAGCGGCTA CCAGTCTGGG TATCACTCAG ACGACACAGA TACCACCGTG TACTCCAGCG 
     4981 ACGAGGCAGG ACTTTTAAAG CTGGTGGATG TTGCAGGGCA CGTTGACTCT GGGACCACAC 
     5041 TGCGCTCATC TCCTGTTCAC GTTGACTCTG GGACCACACT GCGCTCATCT CCTGTTACGC 
     5101 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     5161 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     5221 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     5281 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     5341 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5401 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5461 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5521 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5581 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5641 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5701 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5761 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5821 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5881 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5941 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     6001 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     6061 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     6121 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     6181 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     6241 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     6301 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6361 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6421 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6481 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6541 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6601 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6661 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6721 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6781 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6841 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6901 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6961 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     7021 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     7081 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     7141 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     7201 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     7261 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     7321 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7381 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7441 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7501 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7561 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7621 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7681 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7741 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7801 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7861 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7921 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7981 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     8041 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     8101 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     8161 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     8221 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     8281 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8341 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8401 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8461 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8521 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8581 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8641 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8701 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8761 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8821 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8881 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8941 GCGAATTTT
//