LOCUS       RR204806                8991 bp    DNA     circular  SYN 
DEFINITION  Igf1r (Myc-DDK-tagged ORF) - Rat insulin-like growth factor 1 receptor (Igf1r), (10 ug)
ACCESSION   NM_052807
VERSION     NM_052807.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5148..5239 
                     /label="Myc-DDK"  
     misc_feature    5269..5854 
                     /label="PolyA signal"  
     misc_feature    5326..5345 
                     /label="Restriction Enzyme"  
     misc_feature    6130..6802 
                     /label="ColE1"  
     misc_feature    7232..8026 
                     /label="Kan/Neo"  
     misc_feature    8061..8363 
                     /label="SV40 ori"  
     misc_feature    8449..8926 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5130..5136 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5130 
                     /label="Igf1r"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGTCTGGC TCCGGAGGAG GGTCCCCGAC CTCGCTGTGG GGGCTCGTGT 
     1081 TTCTCTCCGC CGCGCTCTCG CTCTGGCCGA CGAGTGGAGA AATTTGTGGG CCCGGCATTG 
     1141 ACATCCGCAA CGACTATCAG CAGCTGAAGC GCCTGGAAAA CTGCACGGTG ATCGAGGGCT 
     1201 TCCTCCACAT CCTGCTCATC TCCAAGGCCG AGGACTACCG AAGCTACCGC TTCCCCAAGC 
     1261 TCACGGTCAT CACCGAGTAC TTGCTGCTGT TTCGAGTGGC CGGCCTCGAG AGCCTGGGAG 
     1321 ACCTCTTCCC GAACCTCACA GTCATCCGTG GCTGGAAACT CTTCTACAAT TACGCACTGG 
     1381 TCATCTTCGA GATGACCAAT CTCAAGGATA TTGGGCTTTA TAATCTGAGG AACATTACTC 
     1441 GGGGGGCCAT CAGGATTGAG AAAAACGCTG ACCTCTGTTA CCTCTCCACC ATAGACTGGT 
     1501 CTCTCATCTT GGATGCGGTG TCCAATAACT ACATTGTGGG GAACAAGCCC CCAAAGGAAT 
     1561 GTGGGGACCT GTGTCCAGGG ACCTTGGAGG AGAAGCCCAT GTGTGAGAAG ACCACCATCA 
     1621 ACAATGAGTA CAACTACCGC TGCTGGACCA CAAATCGCTG CCAGAAAATG TGCCCAAGTG 
     1681 TGTGTGGGAA GCGAGCCTGC ACCGAGAACA ATGAGTGCTG CCACCCGGAG TGCCTAGGCA 
     1741 GCTGCCACAC ACCGGACGAC AACACAACCT GCGTGGCCTG CCGACACTAC TACTACAAAG 
     1801 GCGTGTGCGT GCCTGCCTGC CCGCCTGGCA CCTACAGGTT CGAGGGCTGG CGCTGTGTGG 
     1861 ACCGGGATTT CTGCGCCAAC ATCCCCAACG CCGAGAGCAG TGACTCAGAT GGCTTCGTCA 
     1921 TCCACGATGG CGAGTGCATG CAGGAGTGTC CATCAGGCTT CATCCGCAAC AGCACCCAGA 
     1981 GCATGTACTG TATCCCCTGT GAAGGCCCCT GCCCCAAGGT CTGCGGCGAT GAAGAAAAGA 
     2041 AAACGAAAAC CATCGATTCT GTGACGTCTG CCCAGATGCT CCAAGGGTGC ACCATTTTGA 
     2101 AGGGCAATCT GCTTATTAAC ATCCGGCGAG GCAATAACAT TGCCTCGGAA TTGGAGAACT 
     2161 TCATGGGGCT CATCGAGGTG GTGACTGGCT ACGTGAAGAT CCGCCATTCC CATGCCTTGG 
     2221 TCTCCTTGTC CTTCCTGAAG AACCTTCGTC TCATCTTAGG AGAGGAGCAG CTAGAAGGGA 
     2281 ACTACTCCTT CTATGTCCTG GACAACCAGA ACTTGCAGCA GCTGTGGGAC TGGAACCACC 
     2341 GGAACCTGAC CGTCAGGTCA GGGAAAATGT ACTTCGCTTT CAATCCCAAG CTGTGTGTCT 
     2401 CTGAAATTTA CCGGATGGAG GAGGTGACAG GAACAAAGGG ACGGCAGAGC AAAGGAGACA 
     2461 TAAACACCAG GAACAACGGA GAGCGAGCTT CCTGTGAAAG TGATGTTCTC CGTTTCACCT 
     2521 CCACCACCAC CTGGAAGAAC CGCATCATCA TAACGTGGCA CCGGTACCGG CCGCCGGACT 
     2581 ACCGGGATCT CATCAGTTTC ACAGTCTACT ACAAGGAGGC ACCCTTTAAA AACGTCACGG 
     2641 AATACGACGG GCAGGATGCC TGTGGCTCCA ACAGCTGGAA CATGGTGGAC GTGGACCTGC 
     2701 CTCCGAACAA GGAGGGGGAG CCTGGCATTT TGCTGCATGG GCTGAAGCCC TGGACCCAGT 
     2761 ATGCAGTCTA TGTCAAGGCT GTGACCCTCA CCATGGTGGA AAACGACCAC ATCCGTGGGG 
     2821 CCAAAAGTGA AATCTTGTAC ATTCGCACCA ACGCTTCAGT TCCTTCCATT CCTCTAGATG 
     2881 TCCTCTCGGC ATCAAACTCC TCCTCTCAGC TGATCGTGAA GTGGAACCCC CCAACTCTGC 
     2941 CCAATGGTAA CTTGAGTTAC TACATTGTGA GGTGGCAGCG GCAGCCGCAG GATGGCTATC 
     3001 TGTTCCGGCA CAACTACTGC TCCAAAGACA AAATACCCAT CAGAAAGTAC GCCGATGGTA 
     3061 CCATCGATGT GGAGGAGGTG ACAGAAAATC CCAAGACAGA AGTGTGCGGT GGTGATAAAG 
     3121 GGCCGTGCTG TGCCTGTCCT AAAACCGAAG CTGAGAAGCA GGCTGAGAAG GAGGAGGCTG 
     3181 AGTACCGTAA AGTCTTTGAG AATTTCCTTC ACAACTCCAT CTTTGTGCCC AGACCTGAGA 
     3241 GGAGGCGGAG AGATGTCCTG CAGGTGGCTA ACACCACCAT GTCCAGCCGA AGCAGGAACA 
     3301 CCACGGTAGC TGACACCTAC AATATCACAG ACCCGGAAGA GTTCGAGACA GAATACCCTT 
     3361 TCTTTGAGAG CAGAGTGGAT AACAAGGAGA GGACTGTCAT TTCCAACCTC CGGCCTTTCA 
     3421 CTCTGTACCG TATCGATATC CACAGCTGCA ACCACGAGGC TGAGAAGCTG GGCTGCAGCG 
     3481 CCTCCAACTT TGTCTTTGCA AGAACCATGC CAGCAGAAGG AGCAGATGAC ATTCCTGGCC 
     3541 CAGTGACCTG GGAGCCAAGA CCTGAAAACT CCATCTTTTT AAAGTGGCCA GAACCCGAGA 
     3601 ACCCCAACGG ATTGATTCTA ATGTATGAAA TAAAATACGG ATCGCAAGTC GAGGATCAGC 
     3661 GGGAATGTGT GTCCAGACAG GAGTACAGGA AGTATGGAGG GGCCAAACTT AACCGTCTAA 
     3721 ACCCAGGGAA CTATACGGCC CGGATTCAGG CTACCTCCCT CTCTGGGAAT GGGTCGTGGA 
     3781 CAGATCCTGT GTTCTTCTAT GTCCCAGCCA AAACAACGTA TGAGAATTTC ATGCATCTGA 
     3841 TCATTGCTCT GCCGGTTGCC ATCCTGCTGA TTGTGGGGGG CCTGGTAATC ATGCTGTATG 
     3901 TCTTCCATAG AAAGAGGAAT AACAGCAGAT TGGGCAACGG GGTGCTGTAC GCCTCTGTGA 
     3961 ACCCCGAGTA TTTCAGCGCA GCTGATGTGT ACGTGCCTGA TGAATGGGAG GTAGCTCGGG 
     4021 AGAAGATCAC CATGAACCGG GAGCTCGGAC AAGGGTCCTT CGGGATGGTC TATGAAGGAG 
     4081 TGGCCAAGGG CGTGGTCAAG GACGAGCCTG AAACCAGAGT GGCCATCAAG ACAGTGAATG 
     4141 AGGCTGCAAG TATGCGTGAG AGAATTGAGT TTCTCAACGA GGCCTCAGTG ATGAAGGAGT 
     4201 TCAACTGTCA CCATGTGGTC CGGTTGCTGG GTGTAGTATC CCAAGGCCAG CCCACCCTGG 
     4261 TCATCATGGA ACTAATGACA CGTGGCGATC TCAAAAGTTA TCTCCGGTCT CTAAGGCCAG 
     4321 AGGTGGAGAA TAATCTAGTC CTGATTCCTC CGAGCTTAAG CAAGATGATC CAGATGGCTG 
     4381 GAGAGATTGC AGATGGCATG GCCTACCTCA ATGCCAACAA GTTCGTCCAC AGAGACCTGG 
     4441 CTGCTCGGAA CTGCATGGTA GCTGAAGATT TCACAGTCAA AATTGGAGAT TTTGGTATGA 
     4501 CACGAGACAT CTACGAGACG GACTACTACC GGAAAGGCGG GAAGGGCTTG CTGCCTGTGC 
     4561 GCTGGATGTC TCCCGAGTCC CTCAAGGATG GCGTCTTCAC CACTCATTCC GATGTCTGGT 
     4621 CCTTTGGGGT CGTCCTCTGG GAGATCGCCA CTCTGGCTGA GCAGCCGTAC CAGGGCCTGT 
     4681 CCAACGAGCA AGTTCTTCGT TTCGTCATGG AGGGCGGCCT TCTGGACAAG CCGGATAACT 
     4741 GCCCCGATAT GCTGTTTGAA CTTATGCGCA TGTGCTGGCA GTACAACCCC AAGATGCGGC 
     4801 CCTCCTTCCT GGAGATCATC GGAAGCATCA AGGATGAGAT GGAGCCCAGT TTCCAGGAGG 
     4861 TCTCCTTCTA CTACAGCGAG GAGAACAAGC CTCCAGAGCC GGAGGAGCTG GAGATGGAGC 
     4921 TGGAGCTGGA GCCCGAGAAC ATGGAGAGCG TCCCGCTGGA CCCTTCGGCC TCCTCAGCCT 
     4981 CCCTGCCTCT GCCTGAAAGA CACTCAGGAC ACAAGGCTGA GAACGGCCCT GGCGTGCTGG 
     5041 TTCTCCGTGC CAGTTTTGAT GAGAGACAGC CTTACGCTCA CATGAATGGG GGACGCGCCA 
     5101 ACGAGAGGGC CTTGCCTCTG CCCCAGTCCT CAACCTGCAC GCGTACGCGG CCGCTCGAGC 
     5161 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     5221 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     5281 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     5341 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     5401 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     5461 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     5521 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     5581 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5641 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5701 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5761 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5821 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5881 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5941 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     6001 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     6061 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     6121 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     6181 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     6241 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     6301 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     6361 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     6421 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     6481 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     6541 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6601 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6661 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6721 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6781 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6841 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6901 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6961 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     7021 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     7081 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     7141 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     7201 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     7261 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     7321 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     7381 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     7441 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     7501 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     7561 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7621 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7681 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7741 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7801 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7861 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7921 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7981 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     8041 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     8101 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     8161 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     8221 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8281 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     8341 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     8401 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     8461 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     8521 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     8581 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8641 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8701 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8761 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8821 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8881 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8941 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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