LOCUS       RR204699                7524 bp    DNA     circular  SYN 
DEFINITION  Manba (Myc-DDK-tagged ORF) - Rat mannosidase, beta A, lysosomal (Manba), (10 ug)
ACCESSION   NM_001031655
VERSION     NM_001031655.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3681..3772 
                     /label="Myc-DDK"  
     misc_feature    3802..4387 
                     /label="PolyA signal"  
     misc_feature    3859..3878 
                     /label="Restriction Enzyme"  
     misc_feature    4663..5335 
                     /label="ColE1"  
     misc_feature    5765..6559 
                     /label="Kan/Neo"  
     misc_feature    6594..6896 
                     /label="SV40 ori"  
     misc_feature    6982..7459 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3663..3669 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3663 
                     /label="Manba"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCATCTCCAC CTGCTCTTTC TGTTGGCGCT GTGCGGGGCA GGCTGTATGG 
     1081 CCGCAGGTCC CAGCTACAGC CTGAGAGGGA GCTGGCGGGT GAGCAACGGG AATAGTTCCC 
     1141 TGGAGCTGCC GGCTACTGTC CCTGGCTATG TGCACAGCGC CCTGCAGCAG CACGGGCTGA 
     1201 TCCAGGATCC TTACTACAGA TTTAATGACC TGAACTACAG ATGGATTTCC TTAGATAACT 
     1261 GGACCTACAG CACAGAATTT AAAATCCCCT TTAATCGCAG TGAATGGCAG AAGGTAAAGT 
     1321 TGATTTTTGA CGGAGTCGAT ACAGTTGCAG AAATCCTGTT CAATAATGTC ACTATTGGGA 
     1381 AGACAGACAA CATGTTCACC AGATATAGCT TTGACGTCAC CAATGTGGTC AAAGACGTGA 
     1441 ACTCCCTAAA GCTGCGGTTC CAGTCAGCAG TGCAGTATGC TGAGTGTCAG AGCAAAGCTC 
     1501 ACACTCAATA CCGGGTGCCC CCAGAGTGCC CCCCAGTGGA ACAGAAGGGT GAATGCCACG 
     1561 TCAACTTCAT CCGAAAGGAG CAGTGCTCCT TCAGCTGGGA CTGGGGGCCT TCCTTTCCCT 
     1621 CTCAAGGAAT CTGGAAAGAC GTTAGAATCG AAGCCTATAA CATCGCTCAT CTGGATCACC 
     1681 TGACATTTTT GCCATTGTAT GATAACACTT CTCAGGCGTG GACTATTGAA ATAGAGGCTT 
     1741 CTTTTGATGT TGTCAGCACA AAGCCAGTTG GTGGTCAGGT GACTATAGCC ATCCCCGAAC 
     1801 TGAAAACACA GCAGGCAAAC CACATTGAAC TTCAACATGG GCAAAGGATT GTTAAACTCC 
     1861 TTGTCAAAAT TAGGAAGGAC GTCACCGTGG AGACCTGGTG GCCTCATGGA CATGGGAACC 
     1921 AGACTGGGTA CAACACGACA ATTCTCTTTG CGCTGGATGG AGGCTTAAAA ATTGAAAAAG 
     1981 CAGCCAAGGT TTATTTCAGA ACAGTGCAAC TTATAGAAGA ACCTATAACA GGGTCTCCTG 
     2041 GTCTGAGTTT CTACTTCAAA ATTAATGGGC TTCCTATATT TCTGAAAGGC TCAAACTGGA 
     2101 TCCCAGCTGA CTCATTCCAA GACAAAGTCA CTTCTGAACT GCTGCAGCTC CTTCTCCAGT 
     2161 CCGCCGTGGA TGCTAACATG AACACTCTGC GCGTGTGGGG GGGTGGCATC TACGAGCAGG 
     2221 ATGAATTCTA TGCACTGTGT GACGAACTTG GAATAATGGT GTGGCAGGAC TTCATGTTTG 
     2281 CCAGTGCCCT CTATCCAACT GAGCCAGGCT TCCTAGAGTC AGTGAGAAAA GAAGTCACTT 
     2341 ACCAGGTCAG GAGACTGAAG TCTCACCCCT CCGTCATCAT CTGGAGTGGT AATAATGAGA 
     2401 ATGAAGTGGC TCTCAGGGTG AACTGGTTCC ATGTAAACCC CAGGGACCTG GGGACTTACA 
     2461 TCAACGACTA TGTGACCCTC TACGTGAAAA CCATCAGGGA GATTGTCTTA TCGGAAGATA 
     2521 GGAGTCGTCC GTTCATTGCA TCCAGCCCAA CCAATGGGGT CAAAACCATG ACGGAAGGCT 
     2581 GGATCTCTAA GGACCCTTAT AGCACCCAGT ATGGCGACAT GCATTTTTAC GACTATTTCA 
     2641 GTGACTGCTG GGACTGGAAG GTCTTTCCAA AAGCTCGATT AGTGTCTGAA TATGGCTACC 
     2701 AGTCCTGGCC TTCCTTCAGC ACACTACAAA AGGTCTCTCG TCAAGAGGAC TGGTCTTACA 
     2761 GCAGCCGCTT CTCCCTTCAT CGGCAGCATC ATGGAAATGG CAACAATGAA ATGCTTCACC 
     2821 AGGTACAGCT TCATTTCCAA CTCCCCCAAA GGAGAGACCC AGTGCGTGCA TTTAAAGATA 
     2881 CCATCTACCT CACTCAGGTG ATGCAGGCCC AGTGTATCAA AACAGAAACC GAGTTCTACC 
     2941 TGCGCAGCCG CAGCGAGATA GTGAATGGAG AAGGCCACAC CATGGGGGCA CTGTACTGGC 
     3001 AGCTGAATGA TATCTGGCAG GCTCCTTCCT GGGCCTCTCT TGAGTACGGA GGGAAATGGA 
     3061 AAATGCTTCA TTACTTCGCT CAGCGTTTCT TCGCTCCGCT GCTGCCAGTC GGTTTTGAAG 
     3121 ATGAAGGCGT TTTTTATGTC TACGGTGTGT CAGATCTTCA CAAGGACTAC CCCACAAAGC 
     3181 TCACCGTGAG GCTCCACCGC TGGAGCTCCC AGAAGCCTTT GTGCACCTTC GTGAGTTTGA 
     3241 GTGCTGTGAT TAAGGCAGGG GAGGCGATGG TTCTGTTCCA GATGCCAGTG TCTAAGCTGC 
     3301 TGAAGAGGTG TAAAGAATGC ACAAGGGACA CCTGTGTGGT TTCCTTTTAC CTCTCAACTG 
     3361 ACAATGAACT CTTCAGTCCA ACCAACTACC ATTTCCTGTC CTCCCTGAAG GATGCCAAAG 
     3421 GGATGGTCAA GGCCAACATC ACTGTCAGCA TCTCTCAGAA GGGTGATCTA TTTGTTTTTG 
     3481 ATCTGAAATC GTCAACATCA GCCATCACTC CCTTTGTTTG GTTGGATGTA GGAAGCATCC 
     3541 CGGGGAGATT TAGTGACAAC GGCTTCCTCA TGATTAAGAA GGAGCTCTCG GTACTGTTTT 
     3601 ACCCATGGAA GCCCACCAGC AAGAGTGAGC TGCAGCAGGC CTTCAGTGTC ACCTCCCTGA 
     3661 CAGATCTGTA CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     3721 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     3781 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     3841 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     3901 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     3961 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4021 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4081 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4141 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4201 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4261 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4321 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4381 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4441 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     4501 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     4561 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     4621 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     4681 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     4741 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     4801 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     4861 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     4921 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     4981 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5041 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5101 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5161 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5221 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     5281 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     5341 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     5401 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     5461 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     5521 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     5581 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     5641 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     5701 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     5761 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     5821 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     5881 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     5941 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6001 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6061 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6121 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6181 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6241 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6301 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     6361 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     6421 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     6481 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     6541 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     6601 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     6661 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     6721 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     6781 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6841 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6901 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     6961 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7021 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7081 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7141 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7201 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7261 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7321 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     7381 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     7441 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     7501 TAACAAAAAT TTAACGCGAA TTTT
//