LOCUS       RR204633                7056 bp    DNA     circular  SYN 
DEFINITION  Brd3 (Myc-DDK-tagged ORF) - Rat bromodomain containing 3 (Brd3), (10 ug)
ACCESSION   NM_001108575
VERSION     NM_001108575.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3213..3304 
                     /label="Myc-DDK"  
     misc_feature    3334..3919 
                     /label="PolyA signal"  
     misc_feature    3391..3410 
                     /label="Restriction Enzyme"  
     misc_feature    4195..4867 
                     /label="ColE1"  
     misc_feature    5297..6091 
                     /label="Kan/Neo"  
     misc_feature    6126..6428 
                     /label="SV40 ori"  
     misc_feature    6514..6991 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3195..3201 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3195 
                     /label="Brd3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCACTACT ACAGCGGCTG CCCCCACGGG GATCCCGGCA GTCCCGGGCC 
     1081 CCGTGAACCC TCCCCCACCT GAGGTCTCCA ACCCCAGCAA GCCCGGCCGA AAGACTAACC 
     1141 AGCTGCAGTA CATGCAGAAC GTAGTGGTGA AGACACTCTG GAAACATCAG TTTGCCTGGC 
     1201 CTTTCTACCA GCCTGTGGAT GCAATCAAGC TGAACCTGCC TGATTATCAT AAAATAATAA 
     1261 AAAACCCAAT GGACATGGGG ACTATCAAGA AGAGACTAGA AAATAATTAT TATTGGAGTG 
     1321 CCAGTGAGTG TATGCAGGAC TTCAACACCA TGTTTACAAA CTGTTATATT TATAATAAGC 
     1381 CCACAGATGA CATAGTGCTA ATGGCCCAGG CCTTAGAGAA AATCTTTCTG CAGAAAGTGG 
     1441 CCCAGATGCC TCAGGAGGAA GTTGAACTAT TGCCCCCTGC TCCAAAGGGC AAAGGCCGGA 
     1501 AGCCAGCTGC AGGAGCCCAG AATGCAGGTG CACAACAAAT GGGAGCTGTG TCCTCGGTCT 
     1561 CCCCAGCACC CCCCTTCCAG AACATACCCC CCACCGTATC CCAGACACCC GTCATTGCCG 
     1621 CCACCCCTGT ACCAACCATC ACTGCAAACG TCACGTCAGT TCCAGTCCCC CCACCCGCCG 
     1681 CACCGCCTCC TCCTGCGACA CCCATCGTCC CAGTGGTCCC TCCCACACCG CCTGTAGTCA 
     1741 AGAAAAAGGG CGTGAAGCGG AAAGCAGACA CAACCACACC CACAACGTCC GCCATCACTG 
     1801 CCAGCCGGAG TGAGTCTCCC CCGCCACTTT CAGAGCCCAA GCAAGCCAAG GTAGTAGCCC 
     1861 GAAGAGAGAG CGGGGGTCGC CCCATCAAAC CTCCCAAGAA GGACCTGGAA GATGGTGAGG 
     1921 TCCCACAGCA CGCGGGTAAA AAGGGAAAAC TGTCCGAGCA CCTGCGGCAT TGTGACAGCA 
     1981 TCCTCCGGGA GATGCTGTCC AAGAAGCACG CTGCCTATGC GTGGCCCTTT TACAAGCCAG 
     2041 TGGATGCCGA GGCACTGGAG CTGCATGACT ACCATGACAT AATCAAACAC CCCATGGACC 
     2101 TTAGCACAGT CAAAAGGAAA ATGGACAGCC GTGAGTACCC AGATGCACAG GGCTTCGCTG 
     2161 CCGATATCCG GTTAATGTTC TCCAATTGTT ATAAGTACAA CCCTCCAGAC CACGAAGTGG 
     2221 TAGCCATGGC CAGGAAGCTC CAGGATGTGT TTGAGATGAG GTTTGCCAAG ATGCCCGATG 
     2281 AGCCCATGGA GGTGCCTGCG CTGCCTGCTC CCACAGCCCC CATCGTGAGC AAAGGGGCTG 
     2341 AGAGCAGCCG TAGTAGCGAG GAGAGCTCTT CGGACTCAGG CAGCTCAGAC TCTGAAGAGG 
     2401 AGCGAGCCAC TCGGCTGGCA GAGCTGCAGG AGCAGCTGAA AGCCGTGCAC GAACAGCTGG 
     2461 CCGCCTTGTC TCAGGCCCCA GTGAACAAAC CAAAGAAGAA GAAGGAGAAG AAGGAAAAGG 
     2521 AGAAGAAGAA GAAAGACAAG GAGAAGGAGA AGGAGAAGCA CAAGGCCAAG TCCGAGGAGG 
     2581 AGAAGAAGGC CAAGGCAGCC CCAGCAGCCA AGCAGGCCCA GCAGAAGAAG GCTCCCACCA 
     2641 AGAAGGCCAA TAGCACAACC ACAGCCAGCA GACAGCTCAA GAAGGGCGGC AAGCAGGCAT 
     2701 CTGCATCCTA TGACTCGGAG GAAGAGGAGG AGGGCCTGCC CATGAGCTAC GATGAGAAGC 
     2761 GGCAACTCAG CCTCGACATC AACCGGCTGC CTGGCGAGAA GCTAGGTCGT GTGGTGCACA 
     2821 TCATTCAGTC CCGGGAGCCT TCCCTTCGGG ACTCGAACCC AGACGAGATT GAGATTGACT 
     2881 TTGAGACCCT GAAGCCAACC ACACTTCGGG AACTGGAGAG ATATGTCAAG TCTTGTTTAC 
     2941 AGAAAAAGCA GAGGAAACCA TTGTCAACAA GCGGGAAGAA GCAGGCAGCC AAATCGAAAG 
     3001 AGGAGCTAGC TCAGGAGAAG AAAAAGGAGC TGGAGAAGCG GCTACAGGAT GTCAGCGGAC 
     3061 AACTGAACAG CAAGAAACCC ACTAAGAAAG AGAAGTCCGG CTCAGCTCCC TCAGGAGGCC 
     3121 CATCAAGGCT CAGCAGTAGC AGCTCCTCCG AGTCTGCGAG TAGCAGCTCC AGTGGGTCAA 
     3181 GCTCTGACAG CAGTGACTCA GAGACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     3241 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     3301 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     3361 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     3421 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     3481 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     3541 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     3601 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     3661 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     3721 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     3781 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     3841 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     3901 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     3961 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     4021 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     4081 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     4141 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     4201 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     4261 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     4321 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     4381 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     4441 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     4501 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     4561 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     4621 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     4681 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     4741 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     4801 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     4861 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     4921 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     4981 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     5041 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     5101 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     5161 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     5221 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     5281 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     5341 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     5401 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     5461 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     5521 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     5581 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     5641 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     5701 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     5761 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     5821 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     5881 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     5941 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     6001 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     6061 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     6121 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     6181 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     6241 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     6301 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6361 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6421 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     6481 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     6541 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     6601 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     6661 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     6721 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     6781 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     6841 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     6901 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     6961 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     7021 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//