LOCUS       RR204616                8466 bp    DNA     circular  SYN 
DEFINITION  Kcnh7 (Myc-DDK-tagged ORF) - Rat potassium voltage-gated channel, subfamily H (eag-related), member 7 (Kcnh7), (10 ug)
ACCESSION   NM_131912
VERSION     NM_131912.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4623..4714 
                     /label="Myc-DDK"  
     misc_feature    4744..5329 
                     /label="PolyA signal"  
     misc_feature    4801..4820 
                     /label="Restriction Enzyme"  
     misc_feature    5605..6277 
                     /label="ColE1"  
     misc_feature    6707..7501 
                     /label="Kan/Neo"  
     misc_feature    7536..7838 
                     /label="SV40 ori"  
     misc_feature    7924..8401 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4605..4611 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4605 
                     /label="Kcnh7"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTGTTCGC AGGGGGCATG TGGCACCGCA AAACACCTTC CTGGGGACCA 
     1081 TCATACGGAA ATTTGAAGGG CAGAATAAAA AATTTATCAT TGCAAATGCC AGAGTGCAGA 
     1141 ACTGTGCCAT CATCTACTGC AATGATGGCT TCTGTGAGAT GACAGGTTTC TCCAGGCCAG 
     1201 ATGTCATGCA GAAGCCATGC ACCTGCGACT TTCTCCATGG GCCTGAGACC AAGAGGCATG 
     1261 ATATTGCCCA GATTGCCCAG GCGCTGTTGG GGTCAGAGGA GAGGAAAGTG GAGGTCACCT 
     1321 ACTATCACAA GAATGGTTCC ACTTTTATTT GTAACACTCA CATAATCCCA GTAAAGAACC 
     1381 AAGAGGGTGT GGCTATGATG TTCATCATTA ACTTTGAGTA TGTGACAGAT GAAGACAATG 
     1441 CTGCCTCCCC AGAGAGAGTC AACCCAATAT TGCCAGTCAA ATCTGTAAAT CGGAAACTTT 
     1501 TTGGGTTCAA ATTTCCTGGT CTGAGAGTTC TAACATACAG AAAGCAGTCC TTGCCGCAGG 
     1561 AAGACCCGGA CGTGGTAGTC ATTGATTCTT CTAAGCACAG CGATGACTCT GTGGCTATGA 
     1621 AGCACTTTAA GTCTCCCACG AAAGAAAGCT GCAGTCCCTC TGAAGCAGAT GATACGAAGG 
     1681 CCTTGATACA GCCTAGCCAG TGTTCTCCCT TAGTGAACAT ATCAGGACCT CTGGACCATT 
     1741 CCTCTCCCAA AAGGCAATGG GACCGCCTCT ACCCTGACAT GCTGCAGTCA AGTTCCCAAC 
     1801 TAACACACTC CAGGTCAAGG GAGAGCCTCT GTAGCATACG GAGAGCATCT TCAGTTCATG 
     1861 ATATAGAAGG GTTCAATGTC CACCCCAAGA ACATATTTAG AGATCGACAT GCCAGTGAAG 
     1921 ACAATGGTCG AAATGTCAAA GGACCTTTCA ATCATATCAA GTCAAGCCTG TTGGGATCCA 
     1981 CATCAGATTC AAACCTCAAC AAATACAGCA CCATTAACAA GATCCCGCAA CTCACTCTGA 
     2041 ACTTTTCAGA TGTCAAAACT GAGAAAAAGA ATACTTCCCC GCCTTCTTCA GACAAAACTA 
     2101 TTATTGCACC CAAGGTTAAA GAGAGGACAC ACAACGTGAC AGAGAAGGTA ACTCAGGTTC 
     2161 TTTCTTTGGG AGCAGATGTC TTGCCAGAAT ACAAGCTGCA GACGCCACGC ATCAACAAAT 
     2221 TTACAATATT GCACTACAGC CCTTTCAAAG CAGTGTGGGA TTGGCTCATT TTACTCCTGG 
     2281 TCATTTATAC TGCTATATTC ACCCCCTACT CGGCAGCCTT TCTCCTCAAC GACAGAGAGG 
     2341 AGCAGAAAAG ACGAGAATGT GGCTATTCTT GTAGCCCTTT GAATGTGGTA GACTTGATTG 
     2401 TGGATATTAT GTTTATTATA GACATCCTAA TAAACTTCAG AACAACCTAT GTAAATCAGA 
     2461 ACGAAGAAGT GGTAAGTGAT CCTGCCAAAA TAGCAGTACA CTACTTCAAA GGCTGGTTCC 
     2521 TGATTGACAT GGTCGCAGCC ATCCCTTTTG ACTTGCTGAT TTTCGGATCA GGCTCTGATG 
     2581 AGACAACAAC ACTAATTGGT CTTTTGAAGA CTGCACGTCT CCTGCGTCTT GTGCGTGTAG 
     2641 CCAGGAAACT GGACCGATAC TCAGAATATG GTGCCGCTGT TCTAATGCTC TTGATGTGCA 
     2701 TATTTGCCCT GATTGCCCAC TGGCTGGCTT GCATCTGGTA TGCGATTGGG AATGTAGAGA 
     2761 GGCCCTATCT GACTGACAAA ATTGGATGGT TGGATTCCTT AGGAACACAA ATTGGGAAAC 
     2821 GTTACAATGA CAGTGACTCG AGTTCTGGAC CGTCCATTAA AGACAAATAC GTCACGGCAC 
     2881 TTTACTTTAC CTTCAGCAGT CTAACCAGTG TAGGATTTGG AAATGTGTCT CCTAACACCA 
     2941 ATTCGGAGAA AATCTTTTCC ATTTGTGTCA TGTTGATTGG CTCTCTAATG TATGCGAGCA 
     3001 TTTTTGGGAA TGTTTCTGCA ATTATTCAAA GACTGTACTC GGGAACTGCC AGGTACCACA 
     3061 TGCAGATGCT GAGAGTGAAA GAGTTCATTC GCTTCCACCA AATCCCCAAC CCTCTGAGGC 
     3121 AACGGCTTGA GGAGTATTTC CAGCACGCAT GGACGTACAC TAACGGCATT GACATGAACA 
     3181 TGGTCTTAAA GGGATTTCCG GAATGTTTAC AAGCTGACAT TTGCCTGCAT CTAAACCAGA 
     3241 CTTTGCTCCA AAACTGCAAA GCCTTTCGAG GAGCAAGTAA AGGTTGCCTC AGAGCTCTGG 
     3301 CAATGAAGTT CAAAACCACC CATGCCCCTC CAGGAGACAC CCTGGTTCAC TGTGGGGACG 
     3361 TCCTAACTGC ACTGTACTTC TTATCCAGAG GCTCCATTGA AATCCTCAAG GATGACATAG 
     3421 TGGTAGCTAT TCTAGGAAAA AATGATATCT TTGGAGAAAT GGTTCATCTT TACGCCAAGC 
     3481 CTGGCAAATC TAATGCAGAT GTGAGAGCGC TCACGTATTG TGACCTGCAT AAGATTCAGC 
     3541 GAGAAGACTT ATTAGAGGTC TTGGATATGT ATCCGGAATT TTCTGATCAC TTTCTGACAA 
     3601 ATCTAGAACT GACTTTCAAC CTGAGACACG AAAGTGCAAA GTCCCAATCC ATAAATGATT 
     3661 CTGAAGGGGA CACCTGTAAG CTTCGAAGAA GGAGATTGTC CTTTGAAAGC GAAGGCGACA 
     3721 AAGATTTTAG CAAAGAAAAC AGTGCAAATG ACGCTGACGA TTCCACAGAT ACAATAAGAC 
     3781 GTTATCAGAG TTCCAAGAAG CACTTCGAAG AGAAGAAAAG CAGATCGTCT TCCTTTATCT 
     3841 CCTCCATCGA TGATGAGCAA AAGCCACTCT TCCTGGGAAC AGTAGATTCC ACTCCAAGAA 
     3901 TGGTGAAAGC AAGCAGGCAC CATGGTGAAG AAGCAGCGCC CCCCTCAGGA AGAATTCACA 
     3961 CAGATAAAAG GAGTCACTCC TGCAAAGATA TCACTGACAC ACACAGCTGG GAAAGAGAGC 
     4021 ATGCCCGGGC TCAGCCTGAA GAATGCAGTC CCTCCGGACT TCAGAGAGCT GCCTGGGGCA 
     4081 TCTCTGAGAC CGAAAGCGAC CTCACCTATG GGGAAGTGGA GCAGAGGTTA GATTTGCTTC 
     4141 AAGAGCAACT TAACAGACTT GAATCCCAAA TGACAACGGA CATCCAGGCC ATCTTACAGC 
     4201 TCCTGCAGAA ACAAACCACC GTAGTCCCTC CAGCCTACAG CATGGTGACC GCAGGAGCAG 
     4261 AGTACCAGAG GCCCATCCTC CGTCTGCTGA GAACCAGTCA CCCTAGAGCA TCCATTAAGA 
     4321 CAGACCGGAG CTTCAGCCCC TCCTCACAAT GTCCTGAATT TCTCGACCTT GAAAAGTCCA 
     4381 AACTCAAATC CAAAGAATCA CTGTCAAGCG GAAAGCGCCT GAACACGGCT TCAGAAGACA 
     4441 ACTTGACTTC ACTTTTAAAA CAAGACAGTG ATGCATCGTC AGAGCTTGAC CCGCGGCAAA 
     4501 GAAAATCTTA CCTTCATCCC ATCCGGCATC CGTCTCTGCC AGACTCCTCC CTAAGCACTG 
     4561 TAGGGATCTT GGGTCTCCAT AGGCATGTTT CTGATCCTGG TCTTCCGGGA AAGACGCGTA 
     4621 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4681 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4741 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4801 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4861 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4921 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4981 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5041 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5101 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5161 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5221 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5281 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5341 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5401 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5461 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5521 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5581 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5641 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5701 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5761 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5821 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5881 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     5941 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6001 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6061 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6121 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6181 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6241 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6301 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6361 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6421 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6481 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6541 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6601 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6661 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6721 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6781 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6841 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6901 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     6961 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7021 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7081 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7141 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7201 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7261 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7321 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7381 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7441 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7501 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7561 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7621 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7681 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7741 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7801 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7861 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7921 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     7981 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     8041 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8101 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8161 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8221 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8281 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8341 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8401 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8461 AATTTT
//