LOCUS       RR204586                8277 bp    DNA     circular  SYN 
DEFINITION  Jak2 (Myc-DDK-tagged ORF) - Rat Janus kinase 2 (Jak2), (10 ug)
ACCESSION   NM_031514
VERSION     NM_031514.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4434..4525 
                     /label="Myc-DDK"  
     misc_feature    4555..5140 
                     /label="PolyA signal"  
     misc_feature    4612..4631 
                     /label="Restriction Enzyme"  
     misc_feature    5416..6088 
                     /label="ColE1"  
     misc_feature    6518..7312 
                     /label="Kan/Neo"  
     misc_feature    7347..7649 
                     /label="SV40 ori"  
     misc_feature    7735..8212 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4416..4422 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4416 
                     /label="Jak2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGAATGGCT TGCCTTACAA TGACAGAAAT GGAGGGAACC TCCACATCTC 
     1081 CTGCACATCA GAATGGTGAT ATTCCTGGAA ATGCTAACTC TGTGAAGCAG ACAGAGCCAG 
     1141 TCCTTCAAGT GTACCTGTAC CATTCTCTTG GGCAAGCTGA AGGAGACTAT CTGAAGTTTC 
     1201 CAAATGGAGA GTATGTTGCC GAAGAAATTT GTGTGGCTGC TTCTAAAGCA TGTGGTATTA 
     1261 CGCCTGTGTA TCATAATATG TTTGCTTTAA TGAGTGAAAC AGAAAGGATC TGGTATCCAC 
     1321 CCAATCATGT CTTCCACATA GATGAGTCAA CCAGGCATAA TATACTCTAC AGAATAAGGT 
     1381 TTTACTTTCC TCATTGGTAC TGCAGTGGCA GCAACAGAAC CTATAGGTAC GGAGTATCTC 
     1441 GTGGGGCTGA AGCTCCTCTC CTTGACGACT TTGTCATGTC TTACCTTTTT GCTCAGTGGC 
     1501 GGCATGATTT TGTTCATGGG TGGATAAAAG TACCTGTGAC TCACGAAACT CAGGAAGAAT 
     1561 GTCTTGGCAT GGCAGTGTTA GACATGATGA GAATAGCTAA GGAGAAGGAC CAGACTCCCC 
     1621 TGGCTGTCTA TAACTCCATC AGCTACAAGA CGTTCTTACC GAAGTGCGTG CGAGCGAAGA 
     1681 TCCAGGACTA TCACATTTTA ACTCGGAAGC GGATAAGGTA CAGATTCCGC AGGTTCATTC 
     1741 AGCAGTTCAG TCAGTGCAAA GCCACTGCCA GGAACCTAAA ACTTAAGTAT CTCATAAACC 
     1801 TGGAGACCCT GCAGTCTGCC TTCTACACAG AACAGTTTGA AGTAAAAGAA TCTGCAAGAG 
     1861 GTCCTTCAGG TGAGGAGATT TTTGCAACCA TTATAATAAC TGGAAACGGT GGAATTCAGT 
     1921 GGTCAAGAGG GAAACATAAG GAAAGTGAGA CACTGACAGA ACAGGATTTA CAGTTGTATT 
     1981 GTGATTTTCC TGATATTATT GATGTCAGTA TTAAGCAAGC AAATCAGGAA TGCTCAACTG 
     2041 AAAGTAGAGT TGTGACCGTC CACAAGCAGG ACGGGAAGGT CTTGGAAATA GAACTTAGCT 
     2101 CATTAAAAGA AGCCTTGTCA TTTGTGTCGT TAATTGACGG GTATTACAGA CTAACTGCGG 
     2161 ATGCACACCA TTACCTCTGT AAAGAGGTGG CTCCTCCAGC CGTGCTTGAA AACATACACA 
     2221 GCAACTGTCA CGGCCCAATT TCAATGGATT TTGCCATCAG CAAACTAAAG AAGGCAGGAA 
     2281 ACCAGACTGG ACTGTATGTA CTTCGATGTA GCCCTAAGGA CTTCAACAAA TACTTCCTGA 
     2341 CCTTTGCCGT TGAGCGAGAA AATGTTATTG AATATAAACA CTGTTTGATT ACAAAGAATG 
     2401 AGAATGGAGA GTACAACCTC AGTGGGACTA AGAGGAACTT CAGTAGTCTT AAGGACCTTT 
     2461 TGAATTGCTA CCAGATGGAA ACTGTGCGCT CAGACAGTAT CATCTTCCAG TTCACCAAAT 
     2521 GCTGTCCTCC AAAGCCGAAA GATAAATCAA ACCTTCTTGT CTTCAGAACA AATGGTGTTT 
     2581 CTGATGTTCA GCTCTCACCA ACATTACAGA GGCATAATAA TGTGAATCAA ATGGTGTTTC 
     2641 ACAAAATCAG GAATGAAGAT TTGATATTTA ATGAAAGCCT TGGCCAAGGC ACTTTTACAA 
     2701 AAATATTTAA AGGTGTAAGA AGAGAAGTTG GAGATTATGG TCAGCTGCAC GAAACCGAAG 
     2761 TTCTTTTGAA AGTCCTAGAT AAAGCACATA GAAACTATTC AGAGTCTTTC TTTGAAGCAG 
     2821 CAAGCATGAT GAGTCAGCTT TCTCACAAGC ATTTGGTTTT GAATTATGGA GTATGTGTCT 
     2881 GTGGAGAGGA GAACATTTTG GTTCAAGAGT TTGTAAAATT TGGATCCCTG GATACATACC 
     2941 TGAAAAAAAA CAAAAATTCC ATAAATATAC TATGGAAACT TGGAGTGGCC AAACAGTTGG 
     3001 CATGGGCCAT GCACTTCCTC GAAGAAAAAT CCCTTATTCA TGGGAATGTG TGTGCCAAAA 
     3061 ATATCCTGCT TATCAGAGAA GAAGACAGGA AAACAGGGAA TCCACCTTTC ATCAAACTTA 
     3121 GTGATCCTGG GATTAGCATC ACAGTTCTAC CAAAGGACAT TCTTCAGGAG AGAATACCAT 
     3181 GGGTACCACC TGAGTGCATT GAAAACCCTA AAAATCTAAC TTTGGCAACA GACAAGTGGA 
     3241 GCTTTGGCAC CACTCTGTGG GAGATCTGCA GTGGAGGAGA CAAGCCTCTG AGTGCTCTGG 
     3301 ACTCTCAAAG AAAGCTGCAG TTCTATGAAG ATAAGCACCA GCTCCCAGCA CCCAAGTGGA 
     3361 CAGAGTTGGC AAACCTTATA AATACTTGCA TGGATTATGA GCCAGACTTC AGGCCTGCCT 
     3421 TCAGAGCTGT CATCCGTGAT CTGAACAGCC TGTTTACTCC AGATTATGAA CTGCTGACAG 
     3481 AAAATGACAT GCTACCCAAC ATGAGAATAG GTGCCCTAGG ATTTTCTGGT GCTTTTGAAG 
     3541 ACAGGGACCC TACACAGTTT GAAGAGAGAC ACTTGAAATT TCTACAGCAA CTTGGCAAGG 
     3601 GTAACTTTGG GAGTGTGGAG ATGTGCCGCT ATGACCCGCT GCAGGACAAC ACTGGAGAGG 
     3661 TGGTAGCTGT TAAGAAGCTC CAGCACAGCA CGGAAGAGCA TCTCCGAGAC TTTGAAAGGG 
     3721 AAATTGAAAT CCTGAAATCC TTGCAGCATG ACAACATCGT GAAGTACAAG GGAGTGTGCT 
     3781 ACAGTGCTGG TCGGCGCAAC CTAAGATTAA TTATGGAATA TTTGCCGTAT GGAAGTTTAC 
     3841 GAGACTATCT CCAAAAACAT AAAGAACGGA TAGATCACAA AAAACTTCTG CAGTATACAT 
     3901 CCCAGATATG CAAGGGCATG GAGTATCTTG GTACAAAAAG GTATATCCAC CGGGATCTGG 
     3961 CAACAAGGAA TATATTGGTA GAAAATGAGA ACAGAGTTAA AATAGGAGAT TTTGGATTAA 
     4021 CCAAAGTCTT GCCACAGGAC AAAGAATACT ACAAAGTAAA GGAACCAGGT GAAAGTCCCA 
     4081 TATTCTGGTA TGCTCCCGAA TCCTTGACAG AGAGCAAGTT TTCTGTGGCC TCAGATGTGT 
     4141 GGAGCTTTGG TGTGGTTCTG TACGAACTTT TCACGTATAT CGAGAAGAGC AAAAGCCCAC 
     4201 CTGTGGAATT TATGCGAATG ATCGGCAATG ATAAACAAGG GCAGATGATC GTATTCCATT 
     4261 TGATAGAGCT ACTGAAGAAC AACGGAAGAC TGCCGAGACC AGAAGGGTGC CCAGACGAGA 
     4321 TTTATGTGAT CATGACAGAA TGCTGGAACA ACAATGTCAA CCAACGTCCC TCTTTCAGGG 
     4381 ACCTTTCCCT TCGGGTGGAT CAAATCCGGG ACAGTATGGC TGCAACGCGT ACGCGGCCGC 
     4441 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4501 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4561 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4621 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4681 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4741 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4801 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4861 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4921 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4981 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5041 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5101 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5161 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5221 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5281 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5341 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5401 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5461 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5521 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5581 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5641 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5701 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5761 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5821 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5881 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5941 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6001 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6061 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6121 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6181 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6241 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6301 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6361 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6421 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6481 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6541 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6601 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6661 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6721 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6781 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6841 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6901 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6961 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7021 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7081 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7141 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7201 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7261 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7321 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7381 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7441 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7501 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7561 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7621 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7681 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7741 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7801 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7861 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7921 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7981 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8041 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8101 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8161 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8221 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//