LOCUS       RR204355                8013 bp    DNA     circular  SYN 
DEFINITION  Dagla (Myc-DDK-tagged ORF) - Rat diacylglycerol lipase, alpha (Dagla), (10 ug)
ACCESSION   NM_001005886
VERSION     NM_001005886.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4170..4261 
                     /label="Myc-DDK"  
     misc_feature    4291..4876 
                     /label="PolyA signal"  
     misc_feature    4348..4367 
                     /label="Restriction Enzyme"  
     misc_feature    5152..5824 
                     /label="ColE1"  
     misc_feature    6254..7048 
                     /label="Kan/Neo"  
     misc_feature    7083..7385 
                     /label="SV40 ori"  
     misc_feature    7471..7948 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4152..4158 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4152 
                     /label="Dagla"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCGGGATC GTGGTGTTCC GGCGGCGCTG GTCTGTGGGC AGCGATGACC 
     1081 TCGTGCTGCC TGCTATCTTC CTCTTCCTGC TGCACACCAC CTGGTTTGTG ATCCTGTCTG 
     1141 TGGTGCTCTT CGGCCTGGTC TACAACCCGC ACGAGGCCTG CTCCCTGAAC CTGGTGGACC 
     1201 ACGGCCGAGG CTACCTGGGC ATCCTTCTGA GCTGTATGAT TGCTGAGATG GCTATCATTT 
     1261 GGCTGAGCAT GCGTGGGGGT ATCCTCTACA CAGAACCCCG GGACTCCATG CAGTACGTGC 
     1321 TCTACGTGCG CCTGGCCATT TTGGTGATCG AGTTCATCTA CGCTATCGTG GGCATCGTCT 
     1381 GGCTTACACA GTACTACACC TCCTGCAATG ACCTCACTGC TAAGAATGTC ACTCTCGGGA 
     1441 TGGTCGTCTG CAACTGGGTG GTCATCCTCA GCGTCTGCAT CACTGTCCTC TGTGTCTTTG 
     1501 ACCCCACTGG CCGCACCTTC GTCAAGCTGA GAGCCACCAA GAGGAGGCAG CGCAACCTGC 
     1561 GGACTTACAA CCTGCGGCAT CGGTTAGAGG AGGGTCAGGC CACCAGCTGG TCCCGCCGTC 
     1621 TAAAAGTGTT CCTCTGTTGC ACCCGGACAA AGGATTCCCA GTCAGATGCC TATTCAGAAA 
     1681 TTGCCTACCT CTTTGCTGAA TTTTTCCGTG ACCTCGACAT AGTGCCCTCC GACATCATTG 
     1741 CTGGCCTGGT GCTGCTTAGG CAGCGGCAGC GAGCCAAGCG CAATGCGGTG CTGGATGAGG 
     1801 CAAACAATGA CATCTTGGCT TTCCTGTCTG GGATGCCAGT GACCAGAAAC ACCAAGTACC 
     1861 TCGACCTCAA GAACTCGCAC GAGATGCTAC GCTACAAAGA AGTCTGCTAC TACATGCTCT 
     1921 TTGCCCTCGC TGCCTATGGC TGGCCCATGT ACCTGATGAG GAAGCCCACC TGTGGCCTCT 
     1981 GCCAGCTGGC TCGGTCCTGC TCGTGTTGTC TCTGCCCTGC ACGCCCCCGA TTCGCCCCTG 
     2041 GGGTCACCAT TGAGGAAGAC AACTGTTGTG GCTGCAATGC AATTGCCATC CGCCGTCACT 
     2101 TCCTGGATGA GAACATGACT GCTGTAGACA TCGTCTATAC CTCCTGTCAT GATGCGGTCT 
     2161 ATGAAACTCC CTTCTACGTA GCTGTGGACC ATGACAAGAA GAAGGTGGTG ATCAGTATCC 
     2221 GGGGAACACT GTCCCCCAAG GATGCCCTGA CTGACCTGAC AGGAGATGCT GAGCGTCTCC 
     2281 CTGTGGAGGG ACACCGAGGC ACCTGGTTGG GCCACAAGGG CATGGTACTC TCAGCTGAGT 
     2341 ACATCAAGAA GAAGCTGGAG CAGGAGATGG TCCTGTCCCA GGCCTTTGGG CGAGACCTGG 
     2401 GCCGTGGAAC CAAACACTAT GGCCTGATTG TGGTAGGCCA CTCCTTGGGA GCAGGCACAG 
     2461 CCGCCATTCT CTCCTTCCTC CTGCGCCCTC AGTACCCGAC CCTCAAGTGC TTCGCCTACT 
     2521 CCCCTCCAGG AGGCCTGCTG AGTGAGGATG CCATGGAGTA CTCCAAAGAG TTTGTGACTG 
     2581 CTGTGGTTCT GGGCAAAGAC CTTGTTCCCA GGATCGGCCT TTCCCAGCTC GAAGGTTTCC 
     2641 GCCGGCAGCT TCTGGATGTC CTGCAGCGAA GCACCAAGCC CAAATGGCGG ATCATCGTGG 
     2701 GGGCCACCAA GTGTATCCCC AAGTCGGAGC TGCCTGAGGA TCAGGTGGAA GTGACTGCCT 
     2761 TGGCCAGCAC ACGGCTCTGG ACACACCCCA GTGATCTGAC CATCGCCCTT TCAGCCAGCA 
     2821 CCCCACTCTA CCCACCTGGC CGTATCATCC ATGTGGTCCA TAACCACCCT GCTGAACAGT 
     2881 GCTGCTGCTG TGAACAGGAA GAGCCCACAT ACTTTGCCAT CTGGGGTGAC AACAAAGCCT 
     2941 TTAATGAGGT GATCATCTCA CCAGCCATGC TCCATGAGCA CCTGCCCTAC GTGGTCATGG 
     3001 AGGGGCTCAA TAAGGTGCTG GAGAATTACA ACAAGGGAAA GACAGCGCTG CTCTCTGCTG 
     3061 CAAAGGTCAT GGTGAGCCCC ACTGAGGTAG ACCTCACTCC TGAGCTCATC TTCCAGCAAC 
     3121 AGCCGCTGCC CACGGGGCCA CCTCTGCCCA CTGGGCTGGC TCTGGAGCTG CCTGCCACAG 
     3181 AGCATCGCAA CAGCAGTGTC AGGAGCAAGT CTCAGTCTGA GATGAGTCTA GAGGGCTTCT 
     3241 CTGAAGGACG GCTGCTGTCC CCAGTGGCTG CTGCCTCAGC AGCCCGGCAA GACCCTGTAG 
     3301 AGCTGTTGCT GCTGTCCACC CAGGAACGAC TGGCCGCGGA GCTGCAGTCC CGGCGGGCAC 
     3361 CACTGGCCAC TATGGAGAGC CTCTCAGACA CTGAGTCACT GTACAGCTTT GACTCACGCC 
     3421 GTTCCTCAGG CTTCCGCAGC ATCAGAGGCT CACCCAGCCT CCATGCAGTA CTGGAGCGTG 
     3481 ATGAGGGCCA CCTGTTTTAC ATCGACCCAG CCATTCCAGA GGAGAACCCA TCCCTCAGCT 
     3541 CCCGCACAGA GCTGCTGGCA GCTGACAGTC TGTCCAAGCA CTCGCAGGAC ACCCAACCCC 
     3601 TGGAGGCAGC ACTAGGCAGC GGGGGTGTGA CTCCTGAGCG ACCACCAAGT GCAGCCAATG 
     3661 ACGAGGAGGA GGCAGCCGGT GGCAGTGAGG GTGGTGGGGT GGCCCCACGA GGAGAGCTGG 
     3721 CACTGCACAA TGGGCGCCTG GGGGATTCGC CCAGCCCTCA GGTGCTAGAA TTCGCTGAGT 
     3781 TCATTGACAG CCTCTTCAAC CTGGACAGCA AGAGCAGCTC CTTCCAGGAC CTGTACTGCA 
     3841 TGATGGTGCC TGAGAGCCCC ACTAGTGACT ACACAGAGGG CCCCAAGTCC CCCAGCCAAC 
     3901 AGGAAATCTT GCTTCGGGCT CAGTTTGAGC CCAATCTGGT ACCCAAGCCC CCCAGGCTCT 
     3961 TTGCAGGATC TGCCGAACCC TCCTCTGGCA TCTCACTCTC ACCCTCCTTC CCACTCAGCT 
     4021 CCTCAGGGGA GCTCATGGAC CTGACACCCA CGGGCCTCAG CAGCCAGGAG TGTCTGGCCA 
     4081 CAGACAAGAT CCGGACTTCC ACCCCCACAG GCCATGGGGC CAGCCCTACC AAGCAGGATG 
     4141 ACCTGGTCAT CTCAGCACGC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4201 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4261 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4321 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4381 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4441 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4501 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4561 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4621 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4681 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4741 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4801 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4861 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4921 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     4981 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5041 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5101 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5161 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5221 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5281 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5341 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5401 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5461 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5521 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5581 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5641 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5701 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5761 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     5821 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     5881 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     5941 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6001 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6061 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6121 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6181 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6241 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6301 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6361 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6421 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6481 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6541 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6601 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6661 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6721 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6781 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     6841 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     6901 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     6961 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7021 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7081 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7141 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7201 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7261 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7321 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7381 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7441 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7501 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7561 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7621 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7681 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7741 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7801 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     7861 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     7921 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     7981 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//