LOCUS       RR204350L3                9449 bp    DNA     circular  SYN 
DEFINITION  Itgb1 (Myc-DDK-tagged ORF) - Rat integrin beta 1 (fibronectin receptor beta) (Itgb1), (10 ug)
ACCESSION   NM_017022
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7740..7920 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    8648..9307 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    7968..8587 
                     /label="ColE1"  
     misc_feature    5780..5803 
                     /label="DDK"  
     misc_feature    6615..6635 
                     /label="LR50"  
     misc_feature    5732..5803 
                     /label="MYC"  
     misc_feature    5813..5878 
                     /label="P2A"  
     misc_feature    5879..6478 
                     /label="Puro"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    3221..3237 
                     /label="V2"  
     misc_feature    6624..7215 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5714..5720 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5714 
                     /label="Itgb1"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GAATTTGCAA CTGGTTTTCT GGATTGGATT 
     3361 GATCAGCTTG ATTTGTTCTG TATTTGGCCA AACAGATAAA AATAGATGTT TAAAAGCAAA 
     3421 TGCCAAATCT TGTGGAGAAT GTATACAAGC AGGGCCAAAT TGTGGGTGGT GTACAAATAC 
     3481 GACATTTTTA CAAGAAGGAA TGCCTACTTC TGCACGATGT GATGATTTAG AAGCTTTGAA 
     3541 AAAGAAGGGT TGCCAACCAA GTGACATAGA GAATCCCAGA GGCTCCCAAA CTATAAAGAA 
     3601 GAATAAAAAC GTCACCAATC GCAGCAAAGG GATGGCAGAG AAGCTCCGGC CAGAAGACAT 
     3661 TACTCAGATC CAACCACAAC AGCTGCTTCT AAAGTTGCGA TCAGGAGAAC CACAGAAGTT 
     3721 TACATTAAAA TTCAAGAGGG CTGAAGACTA CCCTATTGAT CTGTACTACC TTATGGATCT 
     3781 CTCCTACTCT ATGAAAGATG ATCTGGAGAA TGTGAAGAGT CTTGGGACGG ATCTGATGAA 
     3841 TGAAATGAGG AGGATTACTT CAGACTTCCG CATTGGCTTT GGCTCATTTG TGGAGAAAAC 
     3901 TGTGATGCCA TACATTAGTA CAACACCAGC AAAGCTAAGA AATCCTTGTA CGAGTGAACA 
     3961 AAACTGTACC AGCCCATTTA GCTACAAAAA CGTGCTTAGT CTTACTGACA GAGGAGAGTT 
     4021 TTTCAATGAA CTTGTTGGTC AGCAGCGCAT ATCTGGAAAC TTGGACTCTC CAGAAGGTGG 
     4081 CTTTGATGCA ATCATGCAGG TTGCAGTTTG TGGGTCGCTG ATTGGCTGGA GGAATGTAAC 
     4141 ACGACTGCTG GTGTTTTCCA CGGATGCTGG GTTTCACTTT GCTGGAGATG GGAAACTTGG 
     4201 TGGTATTGTT TTACCCAATG ATGGCCAGTG TCACCTGGAA AATAATGTGT ATACAATGAG 
     4261 CCATTACTAT GATTATCCTT CAATTGCTCA CCTTGTTCAG AAACTAAGTG AAAATAATAT 
     4321 TCAGACGATT TTTGCAGTCA CTGAAGAGTT CCAGCCTGTT TACAAGGAAT TGAAGAATTT 
     4381 GATTCCTAAG TCAGCAGTGG GCACACTGTC TGGAAACTCT AGTAATGTGA TACAGCTGAT 
     4441 TATCGATGCC TACAATTCCC TTTCTTCAGA AGTCATTCTG GAAAACAGCA AATTGCCAGA 
     4501 TGGAGTAACA ATAAATTACA AGTCCTACTG CAAGAATGGA GTGAATGGGA CAGGAGAAAA 
     4561 TGGACGAAAG TGCTCTAACA TTTCCATTGG AGATGAGGTT CAATTTGAAA TTAGCATAAC 
     4621 TGCTAATAAA TGTCCAAACA AGGAGTCTGA AACAATTAAA ATTAAACCTC TGGGCTTCAC 
     4681 AGAAGAAGTA GAGGTGGTCC TTCAGTTCAT CTGTAAGTGT AATTGTCAAA GCCATGGCAT 
     4741 CCCAGCAAGT CCCAAGTGCC ATGAGGGAAA TGGGACATTT GAGTGTGGAG CCTGCAGGTG 
     4801 CAATGAGGGT CGTGTTGGGA GACACTGTGA ATGTAGCACA GATGAAGTGA ACAGTGAAGA 
     4861 CATGGATGCT TACTGCAGAA AAGAGAACAG CTCAGAGATC TGCAGTAACA ATGGAGAGTG 
     4921 TGTCTGTGGA CAGTGTGTGT GCAGGAAGAG AGAGAATACA AATGAAATTT ACTCCGGCAA 
     4981 ATTCTGCGAG TGTGATAACT TCAACTGCGA TAGGTCCAAC GGCTTAATCT GTGGAGGAAA 
     5041 TGGTGTGTGC AGGTGTCGTG TCTGTGAATG CTATCCCAAC TACACTGGCA GTGCATGTGA 
     5101 CTGTTCCTTG GACACTGTTC CATGCGTAGC GACAAATGGT CAGATCTGCA ATGGCCGGGG 
     5161 CATCTGTGAA TGTGGTGCTT GTAAGTGCAC AGATCCCAAG TTCCAAGGGC CAACCTGTGA 
     5221 GACCTGTCAG ACCTGCCTTG GTGTCTGTGC AGAGCATAAA GAATGTGTTC AGTGCAGAGC 
     5281 CTTCAATAAA GGAGAAAAAA AAGACACGTG TGCACAGGAG TGCTCCCATT TCAACCTCAC 
     5341 TAAAGTGGAA AGCAGGGAGA AGTTGCCCCA GCCTGTGCAG GTGGACCCTG TGACCCACTG 
     5401 CAAGGAGAAG GACATTGATG ACTGCTGGTT CTATTTCACC TACTCAGTGA ACAGCAACGG 
     5461 TGAAGCTCAC GTGCATGTTG TGGAGACTCC AGACTGTCCT ACTGGTCCCG ACATCATCCC 
     5521 AATTGTAGCA GGCGTGGTTG CCGGAATTGT TCTTATTGGC CTTGCCTTGC TGCTGATTTG 
     5581 GAAACTTTTA ATGATAATTC ATGACAGAAG GGAATTTGCT AAATTTGAAA AGGAGAAAAT 
     5641 GAATGCCAAG TGGGACACGG GTGAAAATCC TATTTACAAG AGTGCCGTGA CAACTGTGGT 
     5701 CAATCCGAAG TATGAGGGAA AAACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA 
     5761 AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTGGGT 
     5821 AGGAAGCGGA GCTACTAACT TCAGCCTGCT GAAGCAGGCT GGAGACGTGG AGGAGAACCC 
     5881 TGGACCTATG ACCGAGTACA AGCCCACGGT GCGCCTCGCC ACCCGCGACG ACGTCCCCAG 
     5941 GGCCGTACGC ACCCTCGCCG CCGCGTTCGC CGACTACCCC GCCACGCGCC ACACCGTCGA 
     6001 TCCGGATCGC CACATCGAGC GGGTCACCGA GCTGCAAGAA CTCTTCCTCA CGCGCGTCGG 
     6061 GCTCGACATC GGCAAGGTGT GGGTCGCGGA CGACGGCGCC GCGGTGGCGG TCTGGACCAC 
     6121 GCCGGAGAGC GTCGAAGCGG GGGCGGTGTT CGCCGAGATC GGCCCGCGCA TGGCCGAGTT 
     6181 GAGCGGTTCC CGGCTGGCCG CGCAGCAACA GATGGAAGGC CTCCTGGCGC CGCACCGGCC 
     6241 CAAGGAGCCC GCGTGGTTCC TGGCCACCGT CGGCGTCTCG CCCGACCACC AGGGCAAGGG 
     6301 TCTGGGCAGC GCCGTCGTGC TCCCCGGAGT GGAGGCGGCC GAGCGCGCCG GGGTGCCCGC 
     6361 CTTCCTGGAG ACCTCCGCGC CCCGCAACCT CCCCTTCTAC GAGCGGCTCG GCTTCACCGT 
     6421 CACCGCCGAC GTCGAGGTGC CCGAAGGACC GCGCACCTGG TGCATGACCC GCAAGCCCGG 
     6481 TGCCTGATGT ACACCCAAAC GGCCGGCCGC GGTCTGTACA AGTAGGATTC GTCGAGGGAC 
     6541 CTAATAACTT CGTATAGCAT ACATTATACG AAGTTATACA TGTTTAAGGG TTCCGGTTCC 
     6601 ACTAGGTACA ATTCGATATC AAGCTTATCG ATAATCAACC TCTGGATTAC AAAATTTGTG 
     6661 AAAGATTGAC TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT 
     6721 TAATGCCTTT GTATCATGCT ATTGCTTCCC GTATGGCTTT CATTTTCTCC TCCTTGTATA 
     6781 AATCCTGGTT GCTGTCTCTT TATGAGGAGT TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG 
     6841 TGTGCACTGT GTTTGCTGAC GCAACCCCCA CTGGTTGGGG CATTGCCACC ACCTGTCAGC 
     6901 TCCTTTCCGG GACTTTCGCT TTCCCCCTCC CTATTGCCAC GGCGGAACTC ATCGCCGCCT 
     6961 GCCTTGCCCG CTGCTGGACA GGGGCTCGGC TGTTGGGCAC TGACAATTCC GTGGTGTTGT 
     7021 CGGGGAAATC ATCGTCCTTT CCTTGGCTGC TCGCCTGTGT TGCCACCTGG ATTCTGCGCG 
     7081 GGACGTCCTT CTGCTACGTC CCTTCGGCCC TCAATCCAGC GGACCTTCCT TCCCGCGGCC 
     7141 TGCTGCCGGC TCTGCGGCCT CTTCCGCGTC TTCGCCTTCG CCCTCAGACG AGTCGGATCT 
     7201 CCCTTTGGGC CGCCTCCCCG CATCGATACC GTCGACCTCG ATCGAGACCT AGAAAAACAT 
     7261 GGAGCAATCA CAAGTAGCAA TACAGCAGCT ACCAATGCTG ATTGTGCCTG GCTAGAAGCA 
     7321 CAAGAGGAGG AGGAGGTGGG TTTTCCAGTC ACACCTCAGG TACCTTTAAG ACCAATGACT 
     7381 TACAAGGCAG CTGTAGATCT TAGCCACTTT TTAAAAGAAA AGGGGGGACT GGAAGGGCTA 
     7441 ATTCACTCCC AACGAAGACA AGATATCCTT GATCTGTGGA TCTACCACAC ACAAGGCTAC 
     7501 TTCCCTGATT GGCAGAACTA CACACCAGGG CCAGGGATCA GATATCCACT GACCTTTGGA 
     7561 TGGTGCTACA AGCTAGTACC AGTTGAGCAA GAGAAGGTAG AAGAAGCCAA TGAAGGAGAG 
     7621 AACACCCGCT TGTTACACCC TGTGAGCCTG CATGGGATGG ATGACCCGGA GAGAGAAGTA 
     7681 TTAGAGTGGA GGTTTGACAG CCGCCTAGCA TTTCATCACA TGGCCCGAGA GCTGCATCCG 
     7741 GACTGTACTG GGTCTCTCTG GTTAGACCAG ATCTGAGCCT GGGAGCTCTC TGGCTAACTA 
     7801 GGGAACCCAC TGCTTAAGCC TCAATAAAGC TTGCCTTGAG TGCTTCAAGT AGTGTGTGCC 
     7861 CGTCTGTTGT GTGACTCTGG TAACTAGAGA TCCCTCAGAC CCTTTTAGTC AGTGTGGAAA 
     7921 ATCTCTAGCA GCATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG 
     7981 TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA 
     8041 AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC 
     8101 TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC 
     8161 CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG 
     8221 GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC 
     8281 TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA 
     8341 GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG 
     8401 AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG 
     8461 AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT 
     8521 GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA 
     8581 GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA 
     8641 GGGATTTTGG TCATGATTAC GCCCCGCCCT GCCACTCATC GCAGTACTGT TGTAATTCAT 
     8701 TAAGCATTCT GCCGACATGG AAGCCATCAC AAACGGCATG ATGAACCTGA ATCGCCAGCG 
     8761 GCATCAGCAC CTTGTCGCCT TGCGTATAAT ATTTGCCCAT GGTGAAAACG GGGGCGAAGA 
     8821 AGTTGTCCAT ATTGGCCACG TTTAAATCAA AACTGGTGAA ACTCACCCAG GGATTGGCTG 
     8881 AGACGAAAAA CATATTCTCA ATAAACCCTT TAGGGAAATA GGCCAGGTTT TCACCGTAAC 
     8941 ACGCCACATC TTGCGAATAT ATGTGTAGAA ACTGCCGGAA ATCGTCGTGG TATTCACTCC 
     9001 AGAGCGATGA AAACGTTTCA GTTTGCTCAT GGAAAACGGT GTAACAAGGG TGAACACTAT 
     9061 CCCATATCAC CAGCTCACCG TCTTTCATTG CCATACGGAA CTCCGGATGA GCATTCATCA 
     9121 GGCGGGCAAG AATGTGAATA AAGGCCGGAT AAAACTTGTG CTTATTTTTC TTTACGGTCT 
     9181 TTAAAAAGGC CGTAATATCC AGCTGAACGG TCTGGTTATA GGTACATTGA GCAACTGACT 
     9241 GAAATGCCTC AAAATGTTCT TTACGATGCC ATTGGGATAT ATCAACGGTG GTATATCCAG 
     9301 TGATTTTTTT CTCCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT 
     9361 GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC 
     9421 GCACATTTCC CCGAAAAGTG CCACCTGAC
//