LOCUS       RR204217                7359 bp    DNA     circular  SYN 
DEFINITION  Kif18b (Myc-DDK-tagged ORF) - Rat kinesin family member 18B (Kif18b), (10 ug)
ACCESSION   NM_001039019
VERSION     NM_001039019.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3516..3607 
                     /label="Myc-DDK"  
     misc_feature    3637..4222 
                     /label="PolyA signal"  
     misc_feature    3694..3713 
                     /label="Restriction Enzyme"  
     misc_feature    4498..5170 
                     /label="ColE1"  
     misc_feature    5600..6394 
                     /label="Kan/Neo"  
     misc_feature    6429..6731 
                     /label="SV40 ori"  
     misc_feature    6817..7294 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3498..3504 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3498 
                     /label="Kif18b"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGATGGCT GTGGAAGACA GCGTGGTTCG GGTGGTCGTG CGGGTGAGGC 
     1081 CTCCCACCCC TAAGGAACTA GAGAGTCAGC GTCGGCCTGT GATTCAGGTG GTAGATGAGC 
     1141 GGATGCTGGT GTTTGACCCC GAGGAGTGTG ATGGGGGATT TCCTGGCCTA AAATGGAGTG 
     1201 GCTCCCTCAG TGGCCCCAAG AAGAAGGGCA AAGACCTGAC ATTTGTCTTT GACCGGGTCT 
     1261 TCAGTGAGGT GGCCACTCAA CAGGATGTGT TCCAGCACAC TACACACAAC ATCCTTGACA 
     1321 GCTTTCTCCA GGGTTACAAC TGCTCAGTGT TTGCCTATGG AGCCACTGGG GCTGGGAAGA 
     1381 CACACACCAT GCTGGGAAGG GAGGGAGACC CTGGCATCAT GTACCTGACC ACCATGGAAC 
     1441 TGTACCGGCG TCTTGAAGCC TGCCAGGAAG AAAAGCAGTT CGAGGTGCTC ATCAGTTACC 
     1501 TGGAGGTGTA TAACGAGCAG ATCTATGACC TCCTGGAGCC CAAAGGACCC CTCACCATCC 
     1561 GAGAGGACCC TGATAAGGGC GTTGTGGTAC CGGGACTTTC TTTCCACCAG CCAGCCTCAG 
     1621 CCCAACAGCT ACTCGAGATG CTGACCAGAG GCAACTGCAG CCGCACACAG CACCCCACAG 
     1681 ATGCCAACGC CACATCTTCC CGGTCTCACG CCATTTTCCA GATCTTTGTG AAGCAGCAGG 
     1741 ACCGGGTTCC AGGACTGACC CAGGCCCTTC GAGTAGCCAA GATGAGCTTG ATTGACCTGG 
     1801 CTGGCTCAGA GCGGGCATCT AGCACCCATG CCAAAGGAGA GCGTCTGCGG GAGGGTGCCA 
     1861 ACATCAACCG CTCTCTGTTA GCACTCATTA ATGTCCTCAA TGCCCTGGCT GATGCAAAGG 
     1921 GCCGCAAGTC TCATGTACCC TACCGAGACA GCAAACTGAC CCGTCTGCTC AAGGACTCCA 
     1981 TTGGGGGCAA CTGCCGCACT GTGATGATTG CAGCGGTCAG CCCTTCCAGC CTGACCTATG 
     2041 AGGATACTTA CAACACCCTC AAATACGCTG ACCGGGCCAA GGAGATCAGA CTCACGCTGA 
     2101 AGAGCAACGT GATCAGCCTG GACCATCACA TCAGTCAGTA TGCCACCATC TGCCAGCAGC 
     2161 TCCAGGCTGA GGTGGCTGCT CTGAGAGAGA AGCTCCAGAC ATATGAGGCT GGAGCCCAGG 
     2221 CCCTGCAACA GCGTTCTCCA CAGCCCCCCA AGTTGAGCAT ACCGCAAAAC CTTTCTAGCT 
     2281 CCCCGTTACA GCCTGGGCAC TCCAGCCAGT CCTGCGCCCC AGAGTTCCAT GCAAAGTATG 
     2341 ACGCTCCTCA AGAGGAGAGC CTGGGAACAG ACTCTCAGGG ACAGGGAACT GTGGAAGAAA 
     2401 ATGCCCCAGA GCAGGAGCAG CCTCCCCAGG ACAAACAGTT TCCAACCCAG ATGCCAGAGC 
     2461 CGAACCTGCC AGGGTCTCCT TGTCCAACTG TGCAGGCAAA GCAAGGCATG AACCAACACT 
     2521 CACTACAGAG ACTGGATGCA GAGCACTCTA AACAGTTGGC CCTCCGGGTG CTACGCCTAG 
     2581 CCCAGCGACA GTACTCCCTG CTGCAGGCGG CCAATCTCCT GACTCCTGAC ATGATCTCAG 
     2641 AGTTTGAGAC CCTGCAGCAG CTGGTGCTTG AGGAGAGCAT GGAGCACGGG GCTGAGAGCC 
     2701 CCAGGTCTCC TGGTCTGGCC AGGGGGATCC CGCTAGCTCA GGAGCTGTGT TCAGAGTCTA 
     2761 AGTCTTCACG GTACTGTGGT CCAGTGACCC GGACCATGGC AAAGCAACTG AGTGGCCTCA 
     2821 CACACACTCT GGGGATCCCA CTGGCACCTG ACTGCACCTC AGATAAGACG TCCCAAAAGC 
     2881 CTACCAAGGA GAAGAAAAGG AAACTGAACC TCGAAGAGCC AGGCAGCCTA CCAGCCCCCA 
     2941 GTGTGGAGAT GAAGCGCCAA CGCCAGTCCT TCCTCCCCTG CCTAAGGAGG GGCTCCCTGC 
     3001 CTAAGATCCA GCCATCCTCT GAGCCCAGAA CTCCCAAAGG AGAGAGGGCC TCTTCTCCCT 
     3061 CCCCCTCCTC CCGAGTCTGC CCAGCCACAG TCATTAAAAG CCGGGTACCC CTGGGCCCTT 
     3121 CTGCCTTACA GAACTGTTCC ACTCCTCTGA CCCTACCCAC TCGTGACCTC AATACCACCT 
     3181 TTAATGTCTC TGAGGAATCC CCCTCCAAAC CCAGCTTCCC CGAACCTATT GACTGGGAGA 
     3241 AAGTTTCCCC GGAGCTGAAC GGCACAGACC AGCCGTTCCT CCCCAGTGCA CCTGTCTTCA 
     3301 TATTCACCAC GAAAGGCCGC AAGCCTTCTC TTCCTACAAC CACTGCCAGC AAGAAGAGGC 
     3361 GCACTGTGAG GCCCTCAGTC TCCCGAGGCC GCAGCTGCAT TGCCCGCCTC CCCAGTAGCA 
     3421 CCTTGAAGAA GCCAGACCGA CCCTTCACAG TCCCAGATCC ACCCTTGAGC CTCCACTGCC 
     3481 TTGATGACCA TCAAGTCATA AGGGACACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     3541 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     3601 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     3661 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     3721 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     3781 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     3841 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     3901 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     3961 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4021 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     4081 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     4141 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     4201 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     4261 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     4321 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     4381 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     4441 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     4501 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     4561 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     4621 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     4681 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     4741 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     4801 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     4861 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     4921 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     4981 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     5041 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     5101 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     5161 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     5221 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     5281 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     5341 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     5401 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     5461 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     5521 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     5581 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     5641 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     5701 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     5761 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     5821 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     5881 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     5941 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     6001 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     6061 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     6121 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     6181 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     6241 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     6301 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     6361 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     6421 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     6481 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     6541 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     6601 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     6661 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     6721 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     6781 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     6841 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     6901 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     6961 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     7021 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     7081 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     7141 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     7201 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     7261 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     7321 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//