LOCUS       RR204194                7455 bp    DNA     circular  SYN 
DEFINITION  Usp5 (Myc-DDK-tagged ORF) - Rat ubiquitin specific peptidase 5 (isopeptidase T) (Usp5), (10 ug)
ACCESSION   NM_001106619
VERSION     NM_001106619.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3612..3703 
                     /label="Myc-DDK"  
     misc_feature    3733..4318 
                     /label="PolyA signal"  
     misc_feature    3790..3809 
                     /label="Restriction Enzyme"  
     misc_feature    4594..5266 
                     /label="ColE1"  
     misc_feature    5696..6490 
                     /label="Kan/Neo"  
     misc_feature    6525..6827 
                     /label="SV40 ori"  
     misc_feature    6913..7390 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3594..3600 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3594 
                     /label="Usp5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGAGCTG AGTGAGGAGG CGCTGCTGTC AGTGTTACCG ACGATCCGTG 
     1081 TCCCCAAGGC GGGAGACCGG GTCCACAAAG ACGAGTGTGC TTTCTCTTTC GACACCCCGG 
     1141 AATCTGAGGG TGGCCTTTAT ATCTGCATGA ACACATTCCT GGGATTTGGG AAACAGTATG 
     1201 TGGAGAGACA TTTCAACAAG ACAGGCCAGC GTGTCTACCT GCACCTTCGG AGGACCCGGC 
     1261 GACCGAAAGA AGAGGACTCC AGCGCAGGCA CTGGAGACCC ACCCCGGAAG AAGCCCACCC 
     1321 GGCTGGCCAT TGGTGTTGAA GGTGGGTTTG ACCTCACCGA GGACAAGTTT GAATATGATG 
     1381 AGGACGTGAA GATTGTCATT TTGCCAGATT ACCTGGAGAT CGCTCGGGAT GGGTTGGGGG 
     1441 GGCTTCCCGA CATTGTCAGA GATCGGGTGA CCAGTGCAGT GGAAGCCCTA CTTTCGGCTG 
     1501 ATTCAGCCTC TCGGAAGCAG GAGGTACAGG CTTGGGATGG GGAAGTCCGT CAGGTGTCTA 
     1561 AGCATGCCTT CAACCTCAAG CAGTTGGACA ACCCCGCTCG GATCCCTCCC TGTGGCTGGA 
     1621 GGTGCTCCAA GTGTGACATG AGAGAGAACC TGTGGCTCAA CCTGACAGAT GGCTCCATCC 
     1681 TGTGCGGCCG GCGCTACTTT GATGGCAGCG GGGGCAACAA CCATGCTGTG GAGCACTACA 
     1741 GGGAGACTGG CTACCCCTTA GCTGTTAAGC TGGGCACCAT CACACCCGAT GGAGCTGATG 
     1801 TGTACTCTTA CGACGAGGAT GACATGGTCC TGGACCCTAG CCTGGCAGAG CACCTGTCTC 
     1861 ACTTTGGCAT CGACATGCTG AAGATGCAGA AGACGGACAA AACAATGACT GAGCTGGAGA 
     1921 TAGACATGAA CCAGCGGATT GGTGAGTGGG AGCTGATCCA GGAGTCAGGC GTGCCACTCA 
     1981 AGCCTCTCTT CGGGCCTGGG TACACAGGCA TCCGGAACCT GGGTAACAGC TGCTACCTCA 
     2041 ACTCCGTGGT CCAGGTGCTC TTCAGCATCC CTGACTTTCA GAGGAAGTAT GTGGATAAAT 
     2101 TGGAGAAGAT CTTCCAGAAT GCCCCAACGG ACCCTACCCA AGACTTCAGC ACCCAGGTGG 
     2161 CGAAGCTGGG GCACGGCCTT CTCTCTGGAG AATATTCTAA GCCAGCACTG GAGTCAGGTG 
     2221 ATGGGGAGCA GGTACCAGAA CAAAAGGAAG TTCAAGATGG CATTGCCCCT CGGATGTTCA 
     2281 AGGCCCTTAT TGGCAAGGGC CACCCTGAGT TCTCCACTAA CCGGCAGCAG GATGCTCAGG 
     2341 AATTCTTCCT ACATCTTATC AACATGGTGG AGAGGAATTG CCGGAGTTCT GAAAATCCAA 
     2401 ATGAAGTGTT CCGCTTCTTG GTGGAAGAGA AGATCAAGTG CCTGGCCACA GAGAAGGTTA 
     2461 AGTACACTCA GCGAGTTGAC TACATCATGC AGTTGCCTGT GCCCATGGAT GCAGCCCTTA 
     2521 ACAAAGAGGA GCTTCTAGAG TATGAAGAGA AGAAGCGGCA AGCAGAAGAG GAGAAAGTAG 
     2581 CACTGCCAGA GCTGGTTCGG GCCCAGGTGC CCTTCAGCTC CTGCCTGGAA GCCTATGGGG 
     2641 CCCCCGAGCA GGTGGATGAC TTCTGGAGTA CAGCTCTACA GGCGAAGTCG GTTGCTGTCA 
     2701 AGACTACACG GTTTGCCTCC TTCCCTGACT ACCTGGTCAT CCAGATCAAG AAGTTCACCT 
     2761 TTGGCTTAGA CTGGGTGCCC AAGAAGCTGG ATGTGTCCAT TGAGATGCCA GAGGAGCTAG 
     2821 ATATTTCCCA GCTGAGGGGC ACAGGGCTAC AGCCCGGAGA GGAGGAGCTG CCTGACATTG 
     2881 CCCCACCCCT GGTCACTCCG GATGAGCCCA AAGGTAGCCT TGGTTTCTAT GGCAACGAAG 
     2941 ACGAAGACTC CTTCTGCTCC CCTCACTTCT CCTCTCCAAC ATCGCCCATG CTGGACGAGT 
     3001 CCGTCGTCAT ACAGTTGGTG GAGATGGGCT TCCCTATGGA CGCCTGCCGG AAAGCCGTGT 
     3061 ACTATACAGG GAACAGTGGT GCTGAAGCAG CCATGAACTG GGTCATGTCA CACATGGATG 
     3121 ACCCAGATTT TGCCAATCCC CTCATCCTGC CTGGGTCCAG TGGGCCTGGC TCCACAAGTG 
     3181 CAGCAGCCGA TCCCCCACCA GAGGACTGTG TGGCCACCAT TGTCTCCATG GGCTTCTCAC 
     3241 GGGACCAGGC CCTGAAAGCT CTACGGGCCA CGAACAATAG CTTAGAGCGG GCTGTAGACT 
     3301 GGATCTTCAG TCACATTGAT GACCTAGATG CAGAAGCTGC TATGGACATC TCAGAGGGAC 
     3361 GCTCAGCTGC TGACTCCATC TCTGAGTCTG TACCAGTGGG ACCTAAAGTC CGGGATGGTC 
     3421 CTGGAAAGTA TCAGCTCTTT GCCTTCATTA GTCACATGGG CACGTCTACT ATGTGTGGTC 
     3481 ACTATGTCTG CCATATCAAG AAGGAAGGCA GATGGGTGAT CTACAATGAC CAGAAAGTGT 
     3541 GTGCCTCGGA AAAGCCACCC AAGGACCTGG GCTACATCTA CTTCTACCAG AGAGTGGTCA 
     3601 GCACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     3661 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     3721 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     3781 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     3841 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     3901 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     3961 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4021 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4081 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4141 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4201 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     4261 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     4321 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     4381 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     4441 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     4501 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     4561 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     4621 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     4681 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     4741 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     4801 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     4861 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     4921 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     4981 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     5041 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     5101 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     5161 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     5221 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     5281 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     5341 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     5401 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     5461 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     5521 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     5581 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     5641 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     5701 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     5761 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     5821 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     5881 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     5941 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6001 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     6061 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     6121 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     6181 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     6241 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     6301 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     6361 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     6421 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     6481 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     6541 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     6601 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     6661 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     6721 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     6781 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     6841 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     6901 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     6961 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7021 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     7081 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     7141 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     7201 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     7261 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     7321 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     7381 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     7441 TTTAACGCGA ATTTT
//