LOCUS       RR204164                6984 bp    DNA     circular  SYN 
DEFINITION  Acss2 (Myc-DDK-tagged ORF) - Rat acyl-CoA synthetase short-chain family member 2 (Acss2), (10 ug)
ACCESSION   NM_001107793
VERSION     NM_001107793.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3141..3232 
                     /label="Myc-DDK"  
     misc_feature    3262..3847 
                     /label="PolyA signal"  
     misc_feature    3319..3338 
                     /label="Restriction Enzyme"  
     misc_feature    4123..4795 
                     /label="ColE1"  
     misc_feature    5225..6019 
                     /label="Kan/Neo"  
     misc_feature    6054..6356 
                     /label="SV40 ori"  
     misc_feature    6442..6919 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3123..3129 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3123 
                     /label="Acss2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGCTTCCC GAGGAGCGGC GCAAGAGCGG CAGCGGGAGC CGGGCTCGCG 
     1081 AGGAGACCGG CGCCGAGGGC CGGGTGCGGG GTTGGTCCCC GCCGCCGGAG GTCAGACGCT 
     1141 CGGCGCACGT CCCCTCTCTG CAGCGCTACC GCGAGCTGCA CCGGCGTTCT GTGGAGGAGC 
     1201 CACGGGAGTT TTGGGGAAAC ATTGCCAAGG AATTTTACTG GAAAACCCCA TGCCCTGGCC 
     1261 CATTCCTCCA GTACAACTTT GATGTGACTA AAGGGAAAAT ATTCACTGAG TGGATGAAAG 
     1321 GAGCAACCAC AAACATCTGC TACAATGTGC TGGATAGAAA TGTCCATGAG AAAAAACTTG 
     1381 GCGACAAAGT TGCTTTTTAC TGGGAGGGCA ATGAGCCAGG GGAGACCACC AAGATCACAT 
     1441 ACCGTGAACT CCTGGTCCAG GTGTGTCAGT TCAGCAATGT TCTCCGTAAA CAGGGTGTTC 
     1501 AGAAGGGTGA CCGAGTGGCC ATCTACATGC CTATGATCTT GGAGCTTGTG GTGGCTATGC 
     1561 TGGCATGTGC TCGCCTTGGA GCTTTGCACT CCATTGTGTT TGCAGGCTTC TCTGCAGAGT 
     1621 CTCTCTGTGA ACGGATCTTG GATTCCAGCT GCTGCCTGCT CATTACTACA GATGCCTTCT 
     1681 ACAGGGGGGA AAAACTTGTG AACCTGAAGG AGCTGGCTGA TGAGTCCTTG GAGAAGTGCC 
     1741 GAGAGAAGGG TTTCCCAGTG AGATGCTGCA TTGTGGTCAA ACATCTGGGG CGGGCAGAGC 
     1801 TGGGCATGAA TGACTCCCCC AGCCAGTCCC CACCCGTTAA GAGGCCATGT CCAGATGTCC 
     1861 AGATCTCCTG GAATGAAGGG GTTGACTTGT GGTGGCATGA ACTCATGCAG CAGGCAGGAG 
     1921 ATGAGTTTGA GCCTGAGTGG TGTGATGCCG AGGACCCTCT CTTCATCTTG TATACCAGTG 
     1981 GCTCCACAGG CAAACCCAAG GGTGTGGTGC ACACAGTTGG AGGCTACATG CTCTATGTGG 
     2041 CTACAACTTT CAAGTATGTG TTTGATTTCC ATCCGGAGGA TGTATTCTGG TGCACAGCAG 
     2101 ACATTGGCTG GATCACTGGT CATTCCTATG TCACCTATGG GCCGCTGGCG AATGGTGCCA 
     2161 CCAGTGTTTT GTTTGAGGGG ATCCCCACAT ACCCAGATGA AGGCCGCTTG TGGAGCATTG 
     2221 TGGACAAATA CAAGGTGACC AAGTTCTACA CGGCACCAAC AGCAATCCGG ATGCTCATGA 
     2281 AGTTTGGAGA TGATCCTGTC ACCAAGCATA GCCGGGCATC CTTGCAGGTG CTGGGCACAG 
     2341 TAGGTGAACC CATCAACCCT GAAGCCTGGC TATGGTACCA TCGGGTAGTA GGTTCCCAGC 
     2401 GTTGCCCCAT TGTAGACACC TTCTGGCAAA CAGAAACAGG TGGCCATATG CTGACCCCTC 
     2461 TCCCTGGTGC CACACCCATG AAACCAGGTT CTGCTTCTTT CCCGTTCTTT GGTGTTGCGC 
     2521 CTGCAATCCT GAACGAGTCG GGGGAAGAAC TGGAAGGGGA AGCTGAAGGT TATCTGGTGT 
     2581 TCAAGCAGCC CTGGCCAGGG ATCATGCGCA CAGTTTATGG GAACCACACA CGTTTCGAGA 
     2641 CCACCTACTT TAAGAAGTTC CCTGGCTACT ACGTGACTGG AGATGGCTGC CGACGGGATC 
     2701 AGGACGGCTA TTACTGGATC ACTGGCAGGA TTGATGACAT GCTCAATGTG TCTGGACATC 
     2761 TTCTGAGTAC AGCAGAGGTG GAATCAGCAC TTGTGGAACA TGAGGCTGTT GCAGAGGCAG 
     2821 CTGTGGTGGG CCACCCTCAT CCTGTGAAGG GTGAATGCCT CTACTGCTTT GTTACCTTGT 
     2881 GTGACGGCCA TACCTTCAGC CCCACCCTCA CTGAGGAACT CAAGAAGCAG ATTAGAGAAA 
     2941 AGATTGGCCC CATTGCCACA CCAGACTACA TCCAGAATGC ACCCGGCTTG CCTAAAACAC 
     3001 GCTCAGGAAA AATCATGAGG AGAGTACTCC GGAAGATTGC TCAGAATGAC CACGACCTGG 
     3061 GGGATACCTC TACAGTGGCT GACCCATCTG TCATCAACCA TCTCTTCAGT CACCGCTGCC 
     3121 TGACCACACA GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     3181 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     3241 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     3301 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     3361 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     3421 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     3481 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     3541 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     3601 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     3661 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     3721 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     3781 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     3841 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     3901 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     3961 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     4021 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     4081 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     4141 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     4201 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     4261 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     4321 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     4381 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     4441 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     4501 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     4561 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     4621 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     4681 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     4741 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     4801 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     4861 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     4921 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     4981 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     5041 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     5101 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     5161 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     5221 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     5281 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     5341 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     5401 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     5461 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     5521 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     5581 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     5641 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     5701 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     5761 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     5821 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     5881 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     5941 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     6001 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     6061 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     6121 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     6181 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     6241 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6301 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6361 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     6421 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     6481 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     6541 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     6601 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     6661 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     6721 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     6781 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     6841 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     6901 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     6961 TAACAAAAAT TTAACGCGAA TTTT
//