LOCUS       RR204145                8340 bp    DNA     circular  SYN 
DEFINITION  Ciita (Myc-DDK-tagged ORF) - Rat class II transactivator (Ciita), (10 ug)
ACCESSION   NM_053529
VERSION     NM_053529.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4497..4588 
                     /label="Myc-DDK"  
     misc_feature    4618..5203 
                     /label="PolyA signal"  
     misc_feature    4675..4694 
                     /label="Restriction Enzyme"  
     misc_feature    5479..6151 
                     /label="ColE1"  
     misc_feature    6581..7375 
                     /label="Kan/Neo"  
     misc_feature    7410..7712 
                     /label="SV40 ori"  
     misc_feature    7798..8275 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4479..4485 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4479 
                     /label="Ciita"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAACCACTTC CAGGCCATCC TGGCCCAGGT GCAGACACTG CTGTCCAGTC 
     1081 AGCAGCCCAG GCAGGTGCGG GCCCTCCTGG ATGGCTTGCT GGAAGAAGAG CTGCTCTCAC 
     1141 GGGAATACCA TTGTGCCCTG CTTCATGAGC CTGATGGTGA GGCTCTGGCC CGGAAGATTT 
     1201 CCCTCACCTT GCTGGAGAAA GGGGACCTGG ACTTGGCGTT TGTGAGCTGG GTTTGCAACA 
     1261 GGCTGCAGGT CCCTGAGGTA GAGAGGGGCC CCAACTGCAG GGACCATGGA GGCCATACTC 
     1321 TGTGTGCCAC CATGGAGCTG GGGTCTCCAG AGGGCAGCTA CCTGGAGCTC CTTAACAGTG 
     1381 ATGCTGACCC CCTACACCTC TACCACCTCT ATGACCAGAT GGACCTTGCT GGGGAGGAAG 
     1441 AGATCGAACT CAACTCAGAG ACAGACATGG ACACCATCAA CTGTGACCAG TTCAGCAAGC 
     1501 TGTTGCAGGA TATGGAAATG GATGAAGAGA CCCGGGAGGC CTATGCCAAT ATCGCGGAAC 
     1561 TGGATCAGTA CGTGTTCCAG GATACCCAGC TGGAGGGCCT GAGCAAGGAC CTCTTCATAC 
     1621 AGCACATTGG ACCAGAGGAA GGCTTTGGTG AGAACGTAGA GGGCCCTGCA GAATCAGGAG 
     1681 AGAAGCCTCA GAAGAGACGC TTCCCAGAAG AGCATGCTAT GGACTCAAAG CACAGGAAAC 
     1741 TAGTGCCTCC CTCTAGGACC TCACTGAACT ATTTGGATCT CCCCGCTGGG CATATCCAGA 
     1801 TCTTCACCAC TCTGCCCCAG GGACTCTGGC AAATCTCAGG GGTTGGCACA GGGCTCTCCA 
     1861 GTGTCCTAGT CTACCACGGT GAGATGCCCC AGGTCAACCA ATTACTCTCT TCAAGCAGCC 
     1921 CCACTGTCCC CAGTCTCCCC GAGTCCCCAG ACCGGCCTGG CTCCACCAGC CCCTTCGCGC 
     1981 CATCCACAGC TGACCTGCCC GGCATGCCTG AACCTGCTCT GACCTCCCGT GTAAATGAGA 
     2041 CAGAGGACAC AACACCCCCC TCCCCATGCC AAGAGGATCC CGAGCCTTCC ATCAAGCTTC 
     2101 CAAAATGGCC AGAGTCCGTG GAGCGGTTCC AGCACTCCCT ACAGGACAAG TACCAAGCAC 
     2161 TGCCCGGGAG CCCAAGTGGT CCTCTGGTAG CCATGGAGCT GGTGCGGGCC AGGTTGGAAA 
     2221 GAGGTAGCAA CAAGAGTCAG GAAAGGGAGC TGGCCACTCC TGACTGGACA GAGCGCCAGC 
     2281 TAGCCCATGG CGGTCTGGCC GAGGTACTTC GGGTTGTCGG CGACTCCAGG AGATCACGAG 
     2341 AGACACAGGT GGTCGCTGTG CTGGGCAAGG CTGGTCAGGG CAAGAGCCAC TGGGCCAGGA 
     2401 CAGTGAGCCA CATTTGGGCA CAAGGCCAGT TACCACAATA TGACTTTGTC TTCTACGTCC 
     2461 CCTGTCAATG CTTGGATCGC CCTGGGGACA CCTACCACCT GCGGGATCTG CTCTGTCCCC 
     2521 CGAACCTGCA GCCACTGGCC ATGGATGACG AGGTCATTGA TCATATTGTG AGGCAGCCGG 
     2581 ACCGTGTTCT GCTCATCCTA GATGCTTTTG AGGAGCTAGA GGCCCAAGAC AGCCTCCTGC 
     2641 ATGGACCCTG TGGACCTCTG TCCCCAGAGC CCTGCTCCCT CCGGGGACTG CTGGCTGGGC 
     2701 TCTTCCAGCG TAAGCTGCTG CGAGGCTGCA CCCTGCTCCT CACAGCCCGG CCCCGGGGCC 
     2761 GCCTGGCTCA GAGCCTGAGC AAGGCAGATG CCATCTTCGA GGTGCCCAGC TTCTCTACGA 
     2821 AACAGGCCCA GATCTATATG AGGCACTACT TTGAGAACTC AGGGACAGCA GGGAGTCACG 
     2881 ACAAGGCCCT GGGCCTCCTG GAGGGCCAGC CTTTTCTCCT CACCCACAGT CACAGCCCTG 
     2941 TTCTGTGCAG TGCCGTGTGT CAGCTCTCCA AGGCCCTGCT GGATCAGGGC GCAGAGGCCC 
     3001 AGCTACCTTG CACACTCACA GGACTCTATG TTGGCCTGCT AGGCCCTGCA GCTCAGAACA 
     3061 GTCCTCCTGG AGCCTTAGTT GAGTTGGCCA AGCTAGCCTG GGAGCTGGGA CGGAGGCACC 
     3121 AAAGCACCTT GCAGGAAACC CAGTTTCCAT CCGTGGAAGT GAAGACCTGG GCTGTGGCCC 
     3181 AAGGCTTGAT GCAGCAGACC ACAGAGGCCC AACTGGCTTT CTCCAGTTTT CTGCTACAGT 
     3241 GCTTCCTGGG TGCTGTGTGG CTGGCACAGT GCAATGAGAT GAAAGACAAG GAGCTGCCAC 
     3301 AGTACCTGGC CTTGACCCCG AGGAAGAAGC GACCCTATGA CAACTGGCTG GAGGGCGTAC 
     3361 CACGCTTTCT GGCTGGCTTA GTTTTCCAGC CTCGAGCCCA CTGCCTGGGA GCTCTGGTGG 
     3421 AGCCTGCAAT GGCTGCAGTG GCAGATAGGA AACGGAAGGT TCTTACCAGG TACCTGAAGC 
     3481 GCTTGAAGCT GGGGACACTT CGGGCCGGGA GGTTGCTGGA GCTGCTACAC TGTGCCCACG 
     3541 AAACACAGGA ACCTGGTATT TGGAAGCATG TTTCACACCA GCTCCCAGGG CACCTCTCCT 
     3601 TCCTGGGCAC ACGGCTCACA GCCCCAGATG TGTATGTTCT GGGCAGGGCC TTGGAGACAG 
     3661 CCAGCCAGGA CTTCTCCTTG GACCTTCGTC AGACTGGCAT CGAGCCCTCC AGACTGGGAG 
     3721 ACCTCGTGGG ACTCAGCTGT GTCACCAGTT TCAGGGCCTC CTTGAGTGAC ACAATGGCAT 
     3781 TATGGGAGTC CCTACAGCAT CAGGGAGAAA CCCAGCTACT CCAGGCGGCA GAGGAGAAGT 
     3841 TCACCATTGA GCCATTTAAG GCCAAATCCC CAAAGGATGT GGAAGACCTG GACAGCCTTG 
     3901 TGCAGACCCA GAGGCTGAGA AACCCCTCAG CGGATGCAGC CAAGGATCTT CCTGCCATCC 
     3961 GGGACCTTAA GAAGCTAGAG TTTGCATTGG GCCCCGTCTT GGGCCCGCAG GCTTTCCCCA 
     4021 CACTGGCCAA GATCCTTCCA GCATTCTCTT CCCTGCAGCA CCTGGACCTG GACTCACTTA 
     4081 GCGAGAACAA GATCGGGGAC AAGGGTGTGT CGAAGCTCTC AGCCACCTTC CCTCAGCTGA 
     4141 AGGCCCTGGA GACGCTCAAC CTGTCCCAGA ATAGCATCAC CGATGTGGGA GCTTGCAAGC 
     4201 TTGCAGAAGC CCTGCCGGCC CTGGCCAAGT CCCTCCTAAG GCTGAGCTTG TACAACAACT 
     4261 GCATCTGTGA CGAAGGGGCC AAGAGCCTGG CACGAGTGCT TCCAGACATG GTGTCCCTCC 
     4321 GGGTGATGGA TGTCCAGTTC AACAAGTTCA CGGCTGTCGG GGCCCAGCAG CTGACCTCCA 
     4381 GCCTTCAGAA GTGCCCTCAG GTGGAGACAC TGGCAATGTG GACACCCACT ATCCCCTTTG 
     4441 GGGTCCAGGA ACACCTGCAG CAGCTGGATG CCCGGATTAG CCTGAGAACG CGTACGCGGC 
     4501 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4561 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4621 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4681 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4741 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4801 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4861 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4921 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4981 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5041 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5101 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5161 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5221 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5281 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5341 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5401 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5461 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5521 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5581 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5641 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5701 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5761 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5821 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5881 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5941 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6001 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6061 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6121 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6181 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6241 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6301 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6361 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6421 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6481 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6541 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6601 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6661 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6721 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6781 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6841 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6901 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6961 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7021 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7081 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7141 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7201 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7261 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7321 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7381 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7441 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7501 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7561 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7621 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7681 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7741 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7801 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7861 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7921 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7981 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     8041 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8101 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8161 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8221 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8281 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//