LOCUS       RR204131                8433 bp    DNA     circular  SYN 
DEFINITION  Atn1 (Myc-DDK-tagged ORF) - Rat atrophin 1 (Atn1), (10 ug)
ACCESSION   NM_017228
VERSION     NM_017228.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4590..4681 
                     /label="Myc-DDK"  
     misc_feature    4711..5296 
                     /label="PolyA signal"  
     misc_feature    4768..4787 
                     /label="Restriction Enzyme"  
     misc_feature    5572..6244 
                     /label="ColE1"  
     misc_feature    6674..7468 
                     /label="Kan/Neo"  
     misc_feature    7503..7805 
                     /label="SV40 ori"  
     misc_feature    7891..8368 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4572..4578 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4572 
                     /label="Atn1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGACACGA CAGAATAAAG ACTCGATGTC AATGAGGAGT GGACGGAAGA 
     1081 AAGAGGCCCC CGGGCCCCGG GAAGAGCTGA GATCGAGGGG CCGGGCCTCC CCTGGAGGGG 
     1141 TCAGCACGTC CAGCAGTGAT GGCAAAGCCG AGAAATCCAG GCAGACAGCC AAGAAGGCCC 
     1201 GAGTAGAGGA GACCTCTACC CCAAAAGCCA ACAAGCAGGG CCGGAGCGAG GAGATCTCAG 
     1261 AGAGTGAAAG CGAAGAGACC AGTGCGCCCA AAAAGACCAA GACCGAGGAG CTTCCGCGCC 
     1321 CACAGTCTCC CTCGGATCTG GACAGCTTAG ATGGCCGCAG CATCAACGAT GACGGCAGCA 
     1381 GTGACCCTAG AGATATAGAC CAGGACAACC GAAGCACATC CCCCAGCATC TACAGCCCTG 
     1441 GCAGTGTGGA AAATGACTCG GATTCATCCT CTGGCCTGTC CCAGGGCCCG GCTCGCCCCT 
     1501 ACCACCCACC TCCACTCTTT CCTCCTTCCC CTCCACCACC AGACAGCATT CCCCGACAGC 
     1561 CAGAGTCTGG CTTTGAACCG CACCCTTCTG TGCCGCCTAC TGGATATCAT GCTCCAATGG 
     1621 AGCCCCCCAC ATCTCGGTTA TTCCAGGGCC CACCTCCTGG AGCTCCTCCC CCACACCCAC 
     1681 AGCTCTACCC TGGGAGTGCT GGTGGAGGTG TTTTATCTGG ACCCCCCATG GGTCCCAAAG 
     1741 GGGGAGCAGC CGCCTCCTCA GTGGGTCCCC CTAGTGGGGG CAAGCAGCAC CCCCCACCCA 
     1801 CTACCCCAAT TCCAATATCA AGCTCTGGGG CCAGTGGCGC TCCTCCAGCA AAGCCCCCCA 
     1861 ATACTCCAGT GGGTGCTGGG AACTTACCTT CTGCTCCACC ACCAGCTACT TTCCCCCACG 
     1921 TGACACCAAA CCTGCCTCCT CCACCTGCCC TGAGACCCCT CAACAATGCC TCAGCCTCTC 
     1981 CTCCTGGCAT GGGGGCTCAG CCAATCCCTG GGCATCTGCC CTCTCCCCAT GCCATGGGGC 
     2041 AGGGTATGAG TGGACTTCCT CCTGGCCCAG AGAAGGGCCC AACCCTGGCC CCCTCTCCCC 
     2101 ACCCTTTACC CCCGGCTTCT TCCTCCGCTC CTGGGCCCCC AATGCGGTAC CCGTATTCAT 
     2161 CCTGCTCCAG TAGCTCTGTT GCAGCGTCTT CTAGTTCCTC CGCCGCCACC TCCCAGTACC 
     2221 CAGCTTCCCA GACCCTGCCC AGTTACCCTC ACTCCTTCCC CCCACCAACA AGTATGTCTG 
     2281 TCTCTAATCA GCCACCCAAG TACACCCAGC CTTCTCTCCC ATCCCAGGCT GTGTGGAGCC 
     2341 AGGGCCCACC TCCTCCTCCT CCTTATGGCC GCCTCTTGCC CAACAACAAC ACTCATCCGG 
     2401 GCCCTTTCCC TCCTACCGGG GGTCAATCCA CAGCCCACCC GCCAGCCCCT GCACATCACC 
     2461 ATCACCAGCA GCAGCAGCAG CCACAGCCAC AGCCACAGCC ACAGCCGCAA CAACATCATC 
     2521 ATGGAAACTC TGGGCCCCCT CCACCTGGGG CATATCCTCA CCCCCTAGAG AGCAGTAACT 
     2581 CCCACCATGC ACACCCTTAC AACATGTCAC CCTCCCTGGG GTCTTTGAGG CCCTACCCAC 
     2641 CAGGGCCAGC ACACCTGCCT CCGTCTCATG GCCAGGTGTC TTATAGCCAA GCAGGTCCCA 
     2701 ATGGTCCCCC GGTTTCTTCT TCTTCCAACT CTTCCGGGTC TTCGTCTCAA GCCGCCTATT 
     2761 CATGCTCACA CCCTTCTTCA TCCCAGGGCC CCCAAGGGGC ATCCTACCCC TTCCCACCAG 
     2821 TTCCTCCAAT CACCACCTCC TCAGCTACTC TTTCCACTGT CATCGCCACC GTGGCTTCCT 
     2881 CGCCAGCAGG TTATAAGACA GCCTCACCAC CTGGCCCCCC TCAGTACAGC AAGAGAGCCC 
     2941 CATCCCCAGG GTCCTACAAG ACAGCCACCC CGCCTGGATA CAAGCCAGGG TCACCACCGT 
     3001 CCTTCAGAAC AGGGACCCCA CCTGGCTATC GAGGCACCTC TCCGCCAGCA GGCCCAGGGA 
     3061 CCTTCAAGCC AGGTTCACCC ACCGTGGGGC CGGGGCCCCT ACCACCCGCG GGGCCTTCAA 
     3121 GTTTGTCATC TCTGCCTCCA CCACCCGCGG CCCCGACTAC AGGGCCGCCC CTGACTGCCA 
     3181 CGCAGATCAA ACAGGAACCA GCTGAAGAGT ATGAAACCCC CGAGAGTCCG GTGCCTCCGG 
     3241 CTCGCAGCCC CTCGCCCCCT CCTAAGGTGG TGGACGTGCC TAGCCATGCC AGCCAGTCAG 
     3301 CCAGGTTCAA TAAGCACCTG GACCGTGGCT TCAACTCGTG CGCGCGCAGC GACCTGTACT 
     3361 TTGTGCCGCT GGAGGGATCC AAGCTGGCCA AGAAGCGCGC GGACCTGGTG GAGAAAGTGC 
     3421 GGCGCGAGGC CGAGCAGCGC GCGCGCGAAG AAAAAGAGCG CGAGCGCGAG CGGGAACGTG 
     3481 AGAAGGAGCG CGAGCGCGAG AAAGAGCGCG AGCTGGAGCG CAGTGTGAAA TTGGCCCAGG 
     3541 AGGGCCGTGC TCCAGTGGAG TGCCCGTCCC TGGGTCCAGT GCCCCATCGG CCTCCCTTTG 
     3601 AACCTGGCAG CGCTGTGGCT ACCGTGCCCC CTTACCTGGG TCCTGATACT CCAGCCCTGC 
     3661 GCACTCTCAG TGAATACGCC CGACCTCATG TCATGTCTCC TGGCAATCGC AACCACCCAT 
     3721 TCTATGTGCC CTTGGGGGCA GTGGACCCGG GGCTTCTGGG TTACAATGTC CCAGCCCTGT 
     3781 ACAGCAGCGA CCCAGCTGCC CGAGAACGGG AGCGGGAAGC CCGTGAACGT GACCTCCGTG 
     3841 ACCGGCTCAA GCCTGGCTTT GAGGTGAAAC CTAGTGAGCT GGAACCCCTA CATGGGGTCC 
     3901 CCGGGCCAGG CCTGGATCCC TTCCCCCGAC ACGGGGGCCT GGCTCTACAA CCTGGGCCAC 
     3961 CTGGCCTGCA TCCTTTCCCT TTTCATCCGA GCCTGGGGCC CCTGGAACGA GAACGGCTAG 
     4021 CGCTGGCAGC TGGGCCAGCC CTGCGTCCTG ACATGTCTTA TGCTGAGCGG TTGGCAGCTG 
     4081 AAAGGCAGCA TGCAGAAAGG GTGGCAGCCC TGGGCAATGA CCCACTAGCC CGGCTGCAGA 
     4141 TGCTCAACGT GACTCCCCAT CATCACCAGC ACTCCCACAT CCACTCTCAC CTACACCTGC 
     4201 ACCAGCAGGA TGCCATCCAT GCGGCCTCCG CCTCGGTGCA CCCTCTCATT GACCCCCTGG 
     4261 CCTCAGGGTC TCACCTTACC CGGATCCCCT ACCCAGCTGG GACCCTCCCT AATCCCCTTC 
     4321 TTCCTCACCC TCTGCACGAG AACGAAGTTC TTCGTCACCA GCTCTTTGCT GCCCCTTACC 
     4381 GGGACCTACC GGCCTCTCTA TCTGCGCCAA TGTCAGCCGC CCATCAGCTG CAGGCCATGC 
     4441 ACGCACAGTC AGCCGAGCTG CAGCGCTTGG CACTGGAACA GCAACAGTGG CTGCACGCTC 
     4501 ATCACCCATT GCACAGCGTG CCGCTGCCTG CCCAGGAGGA CTACTACAGT CACCTGAAGA 
     4561 AGGAGAGTGA CAAGCCGCTG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4621 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4681 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4741 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4801 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4861 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4921 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4981 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5041 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5101 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5161 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5221 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5281 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5341 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5401 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5461 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5521 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5581 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5641 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5701 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5761 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5821 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5881 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5941 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6001 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6061 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6121 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6181 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6241 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6301 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6361 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6421 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6481 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6541 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6601 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6661 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6721 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6781 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6841 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6901 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6961 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7021 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7081 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7141 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7201 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7261 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7321 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7381 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7441 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7501 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7561 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7621 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7681 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7741 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7801 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7861 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7921 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7981 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     8041 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8101 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8161 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8221 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8281 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8341 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8401 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//