LOCUS       RR204042                8874 bp    DNA     circular  SYN 
DEFINITION  Npc1l1 (Myc-DDK-tagged ORF) - Rat NPC1 (Niemann-Pick disease, type C1, gene)-like 1 (Npc1l1), (10 ug)
ACCESSION   NM_001002025
VERSION     NM_001002025.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5031..5122 
                     /label="Myc-DDK"  
     misc_feature    5152..5737 
                     /label="PolyA signal"  
     misc_feature    5209..5228 
                     /label="Restriction Enzyme"  
     misc_feature    6013..6685 
                     /label="ColE1"  
     misc_feature    7115..7909 
                     /label="Kan/Neo"  
     misc_feature    7944..8246 
                     /label="SV40 ori"  
     misc_feature    8332..8809 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5013..5019 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5013 
                     /label="Npc1l1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAGCTGCC TGGCTGGGAT GGCTGCTCTG GGCCCTGCTC CTGAGCGCGG 
     1081 CCCAGGGTGA GCTATACACA CCCAAACACG AAGCTGGGGT CTGCACCTTT TACGAAGAGT 
     1141 GCGGGAAAAA CCCAGAGCTC TCTGGAGGCC TCACGTCACT ATCCAATGTA TCCTGCCTGT 
     1201 CTAACACCCC GGCCCGCCAC GTCACGGGTG AACACCTGGC TCTTCTCCAG CGCATCTGTC 
     1261 CCCGCCTGTA CAACGGCCCC AATACCACTT TTGCCTGTTG CTCTACCAAG CAGCTGCTGT 
     1321 CCTTAGAAAG CAGCATGTCC ATCACCAAGG CCCTTCTCAC GCGCTGCCCG GCCTGCTCTG 
     1381 ACAATTTTGT GAGCTTACAC TGCCACAACA CTTGCAGCCC TGACCAGAGC CTCTTCATCA 
     1441 ACGTCACCCG GGTGGTTGAG CGGGGCGCTG GAGAGCCTCC TGCCGTGGTG GCCTATGAGG 
     1501 CCTTTTATCA GCGCAGCTTT GCTGAGAAGG CCTATGAGTC CTGCAGCCAG GTGCGCATCC 
     1561 CTGCGGCCGC TTCCTTGGCC GTGGGCAGCA TGTGTGGAGT GTATGGCTCC GCCCTCTGCA 
     1621 ATGCTCAGCG CTGGCTCAAC TTCCAAGGAG ACACAGGGAA TGGCCTGGCT CCGCTGGATA 
     1681 TCACCTTCCA CCTCTTGGAG CCTGGCCAGG CCCTACCGGA TGGGATCCAG CCACTGAATG 
     1741 GGAAGATCGC ACCCTGCAAC GAGTCTCAGG GTGATGACTC AGCAGTCTGC TCCTGCCAGG 
     1801 ACTGTGCGGC GTCCTGCCCT GTCATCCCTC CGCCCGAGGC CTTGCGCCCT TCCTTCTACA 
     1861 TGGGTCGCAT GCCAGGCTGG CTGGCCCTCA TCATCATCTT CACTGCTGTC TTTGTGTTGC 
     1921 TCTCTGCAGT CCTTGTGCGT CTCCGAGTGG TTTCCAACAG GAACAAGAAC AAGGCAGAAG 
     1981 GCCCCCAGGA AGCCCCCAAA CTCCCTCATA AGCACAAACT CTCACCCCAT ACCATCCTGG 
     2041 GCCGGTTCTT CCAGAACTGG GGCACAAGGG TGGCCTCGTG GCCACTCACC GTCTTAGCAC 
     2101 TGTCCTTCAT CGTTGTGATA GCCTTAGCAG CAGGCCTGAC CTTTATTGAA CTCACCACAG 
     2161 ACCCTGTGGA ACTGTGGTCG GCCCCCAAGA GCCAGGCCCG GAAAGAGAAG TCTTTCCATG 
     2221 ATGAGCATTT CGGCCCCTTC TTTCGAACCA ACCAGATTTT CGTGACAGCT CGGAACAGGT 
     2281 CCAGCTACAA GTACGACTCC CTACTGCTAG GGTCCAAGAA CTTCAGTGGG ATCCTGTCCC 
     2341 TGGACTTCCT GCTGGAGCTG CTGGAGCTTC AGGAGAGGCT TCGACACCTG CAAGTGTGGT 
     2401 CCCCTGAGGC AGAGCGCAAC ATCTCCCTCC AGGACATCTG CTATGCCCCC CTCAACCCAT 
     2461 ATAACACCAG CCTCTCCGAC TGCTGTGTCA ACAGCCTCCT TCAGTACTTC CAGAACAACC 
     2521 GCACCCTCCT GATGCTCACG GCCAACCAGA CTCTGAATGG CCAGACCTCC CTGGTGGACT 
     2581 GGAAGGACCA TTTCCTCTAC TGTGCAAATG CCCCTCTCAC GTTCAAAGAT GGCACGTCTC 
     2641 TGGCCCTGAG CTGCATGGCT GACTACGGGG CTCCTGTCTT CCCCTTCCTT GCTGTTGGGG 
     2701 GATACCAAGG CACGGACTAT TCCGAGGCAG AAGCGCTGAT CATAACCTTC TCTCTCAATA 
     2761 ACTACCCCGC TGATGATCCC CGCATGGCCC AGGCCAAGCT CTGGGAGGAG GCTTTCTTGA 
     2821 AGGAAATGGA ATCCTTCCAG AGGAACACAA GTGACAAGTT CCAGGTTGCG TTCTCAGCTG 
     2881 AGCGCTCTCT GGAGGATGAG ATCAACCGCA CCACCATCCA GGACCTGCCT GTCTTTGCCG 
     2941 TCAGCTACAT TATCGTCTTC CTGTACATCT CCCTGGCCCT GGGCAGCTAC TCCAGATGCA 
     3001 GCCGAGTAGC GGTGGAGTCC AAGGCTACTC TGGGCCTAGG TGGGGTGATT GTTGTGCTGG 
     3061 GAGCAGTTCT GGCTGCCATG GGCTTCTACT CCTACCTGGG TGTCCCCTCT TCTCTGGTTA 
     3121 TCATCCAAGT GGTACCTTTC CTGGTGCTAG CTGTGGGAGC TGACAACATC TTCATCTTTG 
     3181 TTCTTGAGTA CCAGAGGCTA CCTAGGATGC CTGGGGAACA GCGAGAGGCT CACATTGGCC 
     3241 GCACCCTGGG CAGTGTGGCC CCCAGCATGC TGCTGTGCAG CCTCTCTGAG GCCATCTGCT 
     3301 TCTTTCTAGG GGCCCTGACC CCCATGCCAG CTGTGAGGAC CTTCGCCTTG ACCTCTGGCT 
     3361 TAGCAATTAT CCTCGACTTC CTGCTCCAGA TGACTGCCTT TGTGGCCCTG CTCTCCCTGG 
     3421 ATAGCAAGAG GCAGGAGGCC TCTCGCCCGG ATGTCTTATG CTGCTTTTCA ACCCGGAAGC 
     3481 TGCCCCCACC TAAAGAAAAA GAAGGCCTCT TACTCCGCTT CTTCCGCAAG ATATACGCTC 
     3541 CTTTCCTGCT GCACAGATTC ATCCGCCCTG TTGTGATGCT GCTGTTTCTG ACCCTGTTTG 
     3601 GAGCAAATCT CTACTTAATG TGCAACATCA ACGTGGGGCT AGACCAGGAG CTGGCTCTGC 
     3661 CCAAGGACTC GTACTTGATA GACTACTTCC TCTTTCTGAA CCGATACCTT GAAGTGGGGC 
     3721 CTCCAGTGTA CTTTGTCACC ACCTCGGGCT TCAACTTCTC CAGCGAGGCA GGCATGAACG 
     3781 CCACTTGCTC TAGCGCAGGC TGTAAGAGCT TCTCCCTAAC CCAGAAAATC CAGTATGCCA 
     3841 GTGAATTCCC TGACCAGTCT TACGTGGCTA TTGCTGCATC CTCCTGGGTA GATGACTTCA 
     3901 TCGACTGGCT GACCCCGTCC TCCTCCTGCT GTCGCCTTTA TATACGTGGC CCCCATAAGG 
     3961 ATGAGTTCTG TCCCTCAACG GATACTTCCT TCAACTGCTT AAAAAACTGC ATGAACCGCA 
     4021 CTCTGGGTCC TGTGAGGCCC ACAGCGGAAC AGTTTCATAA GTACCTGCCC TGGTTCCTGA 
     4081 ATGATCCGCC CAATATCAGA TGTCCCAAAG GGGGTCTAGC AGCGTATAGA ACGTCTGTGA 
     4141 ATTTGAGCTC AGATGGCCAG GTTATAGCCT CCCAGTTCAT GGCCTACCAC AAGCCCTTAA 
     4201 GGAACTCACA GGACTTCACA GAAGCTCTCC GGGCGTCCCG GTTGCTAGCA GCCAACATCA 
     4261 CAGCTGACCT ACGGAAGGTG CCTGGGACAG ATCCAAACTT TGAGGTCTTC CCTTACACGA 
     4321 TCTCCAACGT GTTCTACCAG CAATACCTGA CGGTCCTTCC TGAGGGAATC TTCACCCTTG 
     4381 CTCTTTGCTT TGTGCCCACC TTTGTTGTCT GCTACCTCCT ACTGGGCCTG GACATGTGCT 
     4441 CAGGGATCCT CAACCTACTC TCCATCATTA TGATTCTCGT GGACACCATT GGCCTCATGG 
     4501 CTGTGTGGGG TATCAGCTAT AATGCGGTAT CCCTCATCAA CCTTGTCACG GCAGTGGGCA 
     4561 TGTCTGTGGA GTTTGTGTCC CACATCACTC GGTCCTTTGC TGTAAGCACC AAGCCTACCC 
     4621 GGCTGGAGAG GGCTAAAGAT GCTACTGTCT TCATGGGCAG TGCGGTGTTT GCTGGAGTGG 
     4681 CCATGACCAA CTTCCCAGGC ATCCTCATCT TGGGCTTTGC CCAAGCCCAG CTTATTCAGA 
     4741 TCTTCTTCTT CCGCCTCAAC CTTCTGATCA CCTTGCTGGG TCTGCTGCAT GGCCTGGTCT 
     4801 TCCTGCCGGT TGTCCTCAGC TATCTGGGAC CAGATGTTAA CCAAGCTCTG GTACAGGAGG 
     4861 AGAAACTAGC CAGCGAGGCA GCAGTGGCCC CAGAGCCTTC TTGCCCACAG TACCCCTCCC 
     4921 CTGCTGATGC GGATGCCAAT GTTAACTACG GCTTTGCCCC AGAACTTGCC CACGGAGCTA 
     4981 ATGCTGCTAG AAGCTCTTTG CCCAAAAGTG ACCAAAAGTT CACGCGTACG CGGCCGCTCG 
     5041 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     5101 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     5161 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     5221 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     5281 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5341 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5401 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5461 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5521 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5581 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5641 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5701 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5761 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5821 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5881 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5941 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     6001 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     6061 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     6121 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     6181 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     6241 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6301 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6361 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6421 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6481 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6541 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6601 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6661 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6721 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6781 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6841 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6901 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6961 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     7021 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     7081 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     7141 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     7201 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     7261 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7321 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7381 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7441 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7501 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7561 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7621 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7681 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7741 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7801 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7861 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7921 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7981 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     8041 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     8101 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8161 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     8221 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     8281 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8341 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8401 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8461 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8521 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8581 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8641 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8701 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8761 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8821 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//