LOCUS       RR204036                8097 bp    DNA     circular  SYN 
DEFINITION  Gucy2c (Myc-DDK-tagged ORF) - Rat guanylate cyclase 2C (Gucy2c), (10 ug)
ACCESSION   NM_013170
VERSION     NM_013170.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4254..4345 
                     /label="Myc-DDK"  
     misc_feature    4375..4960 
                     /label="PolyA signal"  
     misc_feature    4432..4451 
                     /label="Restriction Enzyme"  
     misc_feature    5236..5908 
                     /label="ColE1"  
     misc_feature    6338..7132 
                     /label="Kan/Neo"  
     misc_feature    7167..7469 
                     /label="SV40 ori"  
     misc_feature    7555..8032 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4236..4242 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4236 
                     /label="Gucy2c"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACGTCACTC CTGGGCTTGG CTGTGCGGTT ACTGCTCTTC CAACCCACGC 
     1081 TGATGTTCTG GGCCTCCCAG GTGAGGCAGA AGTGCCACAA TGGCACCTAC GAGATCAGTG 
     1141 TCCTGATGAT GGATAACTCA GCCTACAAAG AACCTTTGCA AAACTTGAGG GATGCTGTGG 
     1201 AGGAAGGACT GGACATCGTG CGAAAGCGCC TGCGCGAAGC CGAACTAAAT GTGACTGTGA 
     1261 ACGCAACCTT CATCTACTCC GATGGTCTGA TCCATAAGTC AGGTGACTGC CGGAGCAGTA 
     1321 CCTGTGAAGG CCTTGACCTC CTCAGGGAAA TTACAAGAGA TCGTAAGATG GGCTGTGTCC 
     1381 TCATGGGGCC CTCGTGCACG TATTCCACCT TCCAGATGTA CCTTGACACA GAGTTGAACT 
     1441 ATCCCATGAT TTCCGCTGGA AGTTTTGGAT TGTCCTGTGA CTATAAGGAA ACCCTAACCA 
     1501 GGATCCTGCC TCCAGCCAGG AAGCTGATGT ACTTCTTGGT CGATTTCTGG AAAGTCAACA 
     1561 ATGCACCTTT CAAAACCTTT TCCTGGAACT CTTCATATGT TTACAAGAAC GGATCGGAGC 
     1621 CTGAAGATTG TTTCTGGTAC CTCAACGCTC TAGAGGCTGG GGTGTCCTAT TTTTCTGAGG 
     1681 TGCTCAGCTT CAAGGATGTA TTGAGACGCA GTGAACAGTT CCAGGAAATC CTAATGGGCC 
     1741 GTAACAGGAA GAGCAATGTG ATTGTTATGT GTGGCACGCC AGAAACCTTC TACAATGTGA 
     1801 AAGGTGACCT CAAAGTGGCT GACGACACTG TTGTCATCCT GGTAGATCTG TTCAGTAACC 
     1861 ATTACTTTGA GGATGACACC AGAGCTCCTG AGTATATGGA CAATGTCCTC GTCCTGACAC 
     1921 TGCCTCCTGA AAAGTTCATC GCGAACGCCT CTGTCTCTGG GAGGTTTCCA TCGGAAAGAA 
     1981 GCGACTTTTC TCTCGCTTAC TTGGAGGGGA CCTTGCTGTT TGGACACATG CTGCAGACGT 
     2041 TTCTTGAAAA TGGAGAATCT GTCACCACGC CCAAGTTCGC TCGTGCGTTC AGGAATCTCA 
     2101 CTTTTCAAGG CTTAGAGGGG CCCGTGACTC TGGATGACAG TGGGGACATT GACAACATTA 
     2161 TGTGTCTTCT GTATGTGTCT CTGGATACCA GGAAATACAA GGTTCTTATG GCGTATGACA 
     2221 CCCATAAAAA CCAAACGATC CCTGTGGCTA CGAGCCCCAA CTTCATCTGG AAGAACCACA 
     2281 GACTCCCTAA TGACGTTCCT GGGCTGGGCC CTCAAATCCT GATGATTGCC GTCTTCACGC 
     2341 TCACGGGGAT TGTGGTCGTT CTGCTGCTGA TTGCCCTCCT TGTGCTCAGA AAATACAGAA 
     2401 GAGATCATGA ACTTCGACAG AAGAAATGGT CCCACATCCC TTCTGAAAAT ATCTTTCCTC 
     2461 TGGAGACCAA CGAGACCAAC CATGTCAGCC TGAAGATTGA CGATGACAGG AGGCGGGATA 
     2521 CAATCCAGAG AGTGCGACAG TGCAAATACG ACAAGAAGAA AGTGATCCTG AAAGACCTCA 
     2581 AGCACTGTGA TGGTAACTTC AGTGAGAAGC AGAAGATAGA ACTGAACAAG CTACTGCAGT 
     2641 CAGACTACTA CAACCTGACC AAGTTCTACG GCACCGTGAA GCTAGACACC AGGATCTTTG 
     2701 GGGTGGTCGA GTACTGCGAG AGGGGGTCCC TCCGGGAAGT GTTAAATGAC ACGATTTCCT 
     2761 ACCCTGATGG CACGTTCATG GATTGGGAGT TTAAGATCTC TGTCTTAAAT GACATTGCTA 
     2821 AGGGGATGTC CTATCTGCAC TCCAGTAAGA TTGAAGTCCA CGGGCGTCTG AAGTCCACCA 
     2881 ACTGCGTGGT GGACAGTCGC ATGGTGGTGA AGATCACTGA TTTTGGGTGC AATTCCATCC 
     2941 TGCCTCCAAA GAAAGACCTG TGGACTGCCC CCGAGCACCT GCGCCAAGCT ACTATCTCTC 
     3001 AGAAAGGAGA CGTGTACAGC TTCAGCATCA TTGCCCAGGA GATCATCCTC CGCAAGGAAA 
     3061 CTTTCTACAC GCTGAGCTGC CGGGATCAGA ATGAGAAGAT TTTCAGAGTG GAAAATTCCT 
     3121 ATGGGACGAA ACCCTTCCGC CCAGATCTCT TCCTGGAAAC CGCAGATGAG AAGGAGCTGG 
     3181 AGGTCTATCT ATTGGTCAAA AGCTGTTGGG AGGAGGATCC AGAAAAGAGA CCAGATTTCA 
     3241 AGAAAATCGA GAGCACACTA GCCAAGATAT TTGGCCTTTT TCATGACCAA AAAAATGAAT 
     3301 CTTACATGGA CACCTTGATC CGACGTCTAC AGCTGTATTC TCGGAACCTG GAGCACCTGG 
     3361 TGGAGGAAAG GACTCAGCTG TACAAGGCCG AGAGGGACAG GGCTGACCAC CTTAACTTTA 
     3421 TGCTGCTCCC ACGGCTGGTG GTAAAGTCCC TGAAGGAGAA AGGCATCGTG GAGCCAGAGC 
     3481 TGTACGAAGA AGTCACAATC TATTTCAGTG ACATTGTCGG TTTCACGACC ATCTGCAAGT 
     3541 ACAGCACGCC CATGGAGGTG GTGGACATGC TGAATGACAT CTACAAGAGT TTTGACCAGA 
     3601 TTGTGGATCA CCACGACGTC TACAAGGTAG AAACCATCGG CGATGCCTAC GTGGTGGCCA 
     3661 GCGGCCTGCC TATGAGAAAC GGCAACCGGC ATGCAGTGGA CATTTCCAAG ATGGCCTTGG 
     3721 ACATCCTCAG CTTCATGGGG ACCTTTGAGC TGGAGCATCT CCCCGGCCTC CCCGTGTGGA 
     3781 TTCGCATTGG GGTTCATTCT GGCCCCTGTG CTGCTGGTGT GGTGGGGATC AAGATGCCTC 
     3841 GTTATTGCCT GTTTGGAGAC ACTGTCAACA CTGCCTCCAG GATGGAGTCC ACCGGCCTTC 
     3901 CCTTAAGGAT TCACATGAGC AGCTCCACCA TTGCCATCCT GAGGAGAACG GATTGCCAGT 
     3961 TCCTGTACGA AGTGAGGGGA GAAACGTACT TAAAGGGAAG AGGGACCGAG ACCACATACT 
     4021 GGCTGACTGG GATGAAGGAC CAAGAGTACA ACCTGCCAAC CCCACCAACA GTGGAGAACC 
     4081 AACAGCGTCT GCAAACTGAG TTCTCAGACA TGATCGTTAG TGCCTTACAG AAAAGACAGG 
     4141 CCTCGGGCGT GAAGAGCCGG AGGCCCACTC GGGTGGCCAG CTACAAGAAA GGCTTTTTGG 
     4201 AATACATGCA GCTGAACAAC TCAGACCACG ATAGCACCTA TTTTACGCGT ACGCGGCCGC 
     4261 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4321 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4381 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4441 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4501 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4561 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4621 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4681 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4741 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4801 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4861 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     4921 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     4981 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5041 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5101 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5161 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5221 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5281 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5341 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5401 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5461 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5521 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5581 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5641 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5701 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5761 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     5821 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     5881 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     5941 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6001 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6061 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6121 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6181 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6241 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6301 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6361 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6421 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6481 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6541 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6601 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6661 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6721 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6781 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     6841 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     6901 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     6961 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7021 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7081 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7141 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7201 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7261 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7321 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7381 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7441 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7501 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7561 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7621 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7681 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7741 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7801 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     7861 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     7921 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     7981 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8041 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
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