LOCUS       RR203965                6493 bp    DNA     circular  SYN 
DEFINITION  Lmbrd1 (Myc-DDK-tagged ORF) - Rat LMBR1 domain containing 1 (Lmbrd1), (10 ug)
ACCESSION   NM_139189
VERSION     NM_139189.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2650..2741 
                     /label="Myc-DDK"  
     misc_feature    2771..3356 
                     /label="PolyA signal"  
     misc_feature    2828..2847 
                     /label="Restriction Enzyme"  
     misc_feature    3632..4304 
                     /label="ColE1"  
     misc_feature    4734..5528 
                     /label="Kan/Neo"  
     misc_feature    5563..5865 
                     /label="SV40 ori"  
     misc_feature    5951..6428 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2632..2638 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2632 
                     /label="Lmbrd1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCGGCG GCTGCCGATC TGGTGATCGG CTGGTGCATC TTCGGCCTCT 
     1081 TGCTCCTGGC TATTTTGGCC TTTTGCTGGG TATACGTTCG TAAATACCAG AGCCAACGGG 
     1141 AAAGTGAGGT CGTCTCCACT GTGACAGCCA TTTTTTCACT GGCTGTCGCG CTTATCACAT 
     1201 CAGCGCTCCT GCCAGTGGAT ATATTTTTGG TTTCTTACAT GAAAAATCAA AATGGCACAT 
     1261 TCAAGGACTG GGCTAATGCC AATGTCACTG TACAGATTGA GAACACAGTT CTGTATGGCT 
     1321 ACTATACTCT GTACTCCGTC ATCCTGTTCT GTGTGTTCTT CTGGATCCCC TTTGTCTACT 
     1381 TCTACTATGA GGAGAAGGAC GACGACGACA CCAGCAAGTG CACCCAAGTT AAAACGGCGC 
     1441 TCAAGTACAC ACTGGGATTT GTTGTGATCT GCGCGCTGCT GCTCCTTGTT GGTGCCTTTG 
     1501 TGCCGCTGAA TCTGCCCAAC AACAACAATT CTACGGAGTG GGAAAAGGTG AAGCTCCTGT 
     1561 TTGAAGATCT CGGAACTGGT CAAGGTCTGG CCGCGCTGTC CTTCTCCATC AGCTCTCTGA 
     1621 CCTTGATTGG GATGCTGGCG GCCATAACAT ACACAGCGTA TGGCATGTCG GCATTACCTT 
     1681 TAAACCTGAT AAAAGGCACT AGGAGCACTG CCTACGAACG CCTGGAGAAC ACTGAGGACA 
     1741 TCGAGGAGGT GGAGCAGCAC ATCCAGACGA TTCGATCAAA GAGCAAAGAT GGCCGACCTT 
     1801 TGTCCGCGAG GGATAGACGT ACCTTAAAGC AATGTGAAGA AAGATTAAGA ACACTTAGGA 
     1861 AAAGAGAACG GCACTTAGAA TTCATTGAAA ACAGCTGGTG GACAAAATTC TGTGGTGCCC 
     1921 TGCGCCCCCT GAAGATTATT TGGGGAATAT TTTTCATCCT GGTTGCATTG CTGTTTGTAA 
     1981 TTTCTCTCTT CCTGTCAAAT TTAGATAAAG CTCTTCACTC AGCGGGAATA GATTCGGGTT 
     2041 TCATCGTCTT TGGAACTAAC CTGAGCAATC CATTGAACAT GCTTTTGCCT TTGTTACAAA 
     2101 CCGTGTTCCC TCTTGATTAT ATTCTTATAA CAATTATTAT TATGTACTTT ATTTTTACTT 
     2161 CGATGGCAGG AATTCGAAAT ATTGGCATTT GGTTCTTTTG GATTAGACTA TATAAAATCA 
     2221 GAAGAGGACG AACCAGGCCT CAGGCGCTCT TATTTCTCTG CATGATTCTT CTGCTGATTG 
     2281 TCCTCCACAC CAGCTACATG ATCTATAGTC TTGCTCCTCA GTACGTGATG TACGGAAGCC 
     2341 AGAATTACTT AATAGAGAGC AATATAACTT CTGATGCCCA TAAAGGCAAC TCAACGCTTG 
     2401 CTGTGCCAAA GAGATGTGAT GCAGATGCCC CAAAAGATCA GTGTACTGTC ACCCGAACAT 
     2461 ACGTATTCCT CCACAAGTTC TGGTTCTTCA GCGCCGCATA CTACTTTGGT AACTGGGCCT 
     2521 TTCTGGTGGT GTTCTTGATC GGATTGATTG TATCCTGCTG TAAAGGGAAG AAGTCAGTCA 
     2581 TAGAAGGAGT GGATGAAGAT TCAGACTTAA GCGATGACGA ACCGTCCGCC TACTCTGCAA 
     2641 ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT 
     2701 ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT 
     2761 TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT 
     2821 GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT 
     2881 GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG 
     2941 GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC 
     3001 AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC 
     3061 TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT 
     3121 TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA 
     3181 GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC 
     3241 CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA 
     3301 TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT 
     3361 CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG 
     3421 CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG 
     3481 GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC 
     3541 TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC 
     3601 AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC 
     3661 TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT 
     3721 CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG 
     3781 TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT 
     3841 CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG 
     3901 CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT 
     3961 GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC 
     4021 CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA 
     4081 GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG 
     4141 ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA 
     4201 TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA 
     4261 GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC 
     4321 CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG 
     4381 AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC 
     4441 CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC 
     4501 TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA 
     4561 GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC 
     4621 AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT 
     4681 CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC 
     4741 CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC 
     4801 GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC 
     4861 ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT 
     4921 GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT 
     4981 CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG 
     5041 CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG 
     5101 AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC 
     5161 AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG 
     5221 GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC 
     5281 TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG 
     5341 CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG 
     5401 AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG 
     5461 TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA 
     5521 TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA 
     5581 AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG 
     5641 CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC 
     5701 TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT 
     5761 TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG 
     5821 TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT 
     5881 TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT 
     5941 TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA 
     6001 ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC 
     6061 TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC 
     6121 CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC 
     6181 CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT 
     6241 TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG 
     6301 GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG 
     6361 TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT 
     6421 ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT 
     6481 TAACGCGAAT TTT
//