LOCUS       RR203938                8538 bp    DNA     circular  SYN 
DEFINITION  Jag1 (Myc-DDK-tagged ORF) - Rat jagged 1 (Jag1), (10 ug)
ACCESSION   NM_019147
VERSION     NM_019147.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4695..4786 
                     /label="Myc-DDK"  
     misc_feature    4816..5401 
                     /label="PolyA signal"  
     misc_feature    4873..4892 
                     /label="Restriction Enzyme"  
     misc_feature    5677..6349 
                     /label="ColE1"  
     misc_feature    6779..7573 
                     /label="Kan/Neo"  
     misc_feature    7608..7910 
                     /label="SV40 ori"  
     misc_feature    7996..8473 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4677..4683 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4677 
                     /label="Jag1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGGTCCCCA CGGACGCGCG GCCGGCCGGG GCGCCCCTTG AGCCTTCTGC 
     1081 TCGCCCTGCT CTGTGCCCTG CGAGCCAAGG TGTGCGGGGC CTCGGGTCAA TTTGAGCTGG 
     1141 AGATCCTGTC CATGCAGAAC GTGAACGGAG AGTTACAGAA TGGGAACTGT TGCGGCGGAG 
     1201 CCCGGAACCC TGGCGACCGC AAGTGCACCC GCGACGAGTG TGACACGTAC TTCAAAGTGT 
     1261 GCCTCAAGGA GTATCAGTCC CGCGTCACGG CCGGGGGACC CTGCAGCTTC GGCTCAGGGT 
     1321 CTACGCCTGT CATCGGGGGC AATACCTTCA ATCTCAAGGC CAGCCGCGGC AACGACCGTA 
     1381 ATCGCATCGT ACTGCCTTTC AGTTTCGCCT GGCCGAGGTC CTACACTTTG CTGGTGGAGG 
     1441 CATGGGATTC CAGTAATGAC ACTATTCAAC CTGATAGCAT AATTGAAAAG GCTTCTCACT 
     1501 CAGGCATGAT AAACCCCAGC CGGCAGTGGC AGACACTGAA ACAGAACACA GGGATTGCCC 
     1561 ACTTTGAGTA TCAGATCCGA GTGACCTGTG ATGACCACTA CTATGGCTTT GGCTGCAACA 
     1621 AGTTCTGTCG GCCCAGAGAT GACTTCTTTG GACATTATGC CTGCGACCAG AATGGCAACA 
     1681 AAACCTGCAT GGAAGGCTGG ATGGGTCCTG AGTGTAACAA AGCAATATGC CGACAGGGCT 
     1741 GCAGTCCCAA GCATGGCTCC TGCAAACTCC CAGGTGACTG CAGGTGCCAG TATGGTTGGC 
     1801 AGGGCCTGTA CTGTGATAAG TGCATCCCGC ACCCAGGATG TGTCCACGGC ACCTGCAATG 
     1861 AACCCTGGCA GTGCCTCTGT GAAACCAACT GGGGTGGACA GCTCTGTGAC AAAGATCTGA 
     1921 ATTACTGTGG GACTCATCAG CCCTGTCTCA ACCGGGGAAC ATGTAGCAAC ACTGGCCCTG 
     1981 ACAAATACCA GTGTTCCTGC CCCGAGGGCT ACTCAGGACC GAACTGTGAA ATTGCTGAGC 
     2041 ATGCCTGCCT CTCTGATCCC TGCCACAACA GAGGCAGCTG CAAGGAGACC TCCTCGGGCT 
     2101 TTGAGTGTGA GTGTTCTCCA GGCTGGACTG GACCCACGTG TTCCACAAAC ATCGATGACT 
     2161 GTTCTCCAAA TAACTGTTCC CATGGAGGCA CCTGCCAGGA TCTAGTGAAT GGATTCAAGT 
     2221 GCGTGTGCCC ACCCCAGTGG ACTGGCAAGA CTTGTCAGTT AGATGCAAAT GAGTGCGAGG 
     2281 CCAAGCCTTG TGTAAATGCC AGATCCTGTA AGAATCTGAT TGCCAGCTAC TACTGTGATT 
     2341 GTCTTCCTGG CTGGATGGGT CAGAATTGTG ACATAAATAT CAATGACTGC CTTGGCCAGT 
     2401 GTCAGAATGA TGCCTCCTGT CGGGATTTGG TTAATGGTTA TCGCTGTATC TGTCCACCTG 
     2461 GCTATGCGGG CGATCACTGT GAAAGAGACA TCGATGAATG TGCTAGCAAC CCCTGCTTGA 
     2521 ATGGGGGTCA CTGTCAGAAT GAAATTAACA GATTCCAGTG TCTGTGTCCC ACTGGTTTCT 
     2581 CTGGAAACCT CTGTCAGCTG GACATCGATT ACTGCGAGCC CAACCCTTGC CAGAATGGCG 
     2641 CCCAATGCTA CAATCGTGCC AGTGACTATT TCTGCAAGTG CCCTGAGGAC TACGAGGGCA 
     2701 AGAACTGCTC ACACCTGAAA GACCACTGCC GCACCACTCC CTGTGAAGTG ATTGACAGCT 
     2761 GCACCGTGGC CATGGCCTCC AACGATACTC CTGAAGGGGT GCGGTATATC TCTTCCAACG 
     2821 TCTGTGGTCC CCATGGGAAG TGCAAGAGCG AGTCAGGAGG CAAATTCACC TGTGACTGTA 
     2881 ACAAAGGCTT CACCGGCACC TACTGCCATG AAAATATCAA CGACTGTGAG GGCAACCCCT 
     2941 GTACGAACGG TGGCACGTGC ATCGACGGCG TTAACTCCTA CAAGTGTATC TGTAGTGACG 
     3001 GCTGGGAGGG CGCGCACTGT GAGAACAACA TAAATGACTG TAGCCAGAAC CCTTGCCACT 
     3061 ATGGGGGTAC ATGTCGAGAC CTGGTCAATG ACTTTTACTG TGACTGCAAA AATGGGTGGA 
     3121 AAGGGAAAAC CTGCCACTCG CGTGACAGCC AGTGTGACGA AGCCACGTGT AATAACGGTG 
     3181 GTACCTGCTA TGATGAAGTG GATACGTTTA AGTGCATGTG TCCTGGTGGC TGGGAAGGAA 
     3241 CAACCTGTAA TATAGCTAGA AACAGTAGCT GCCTGCCGAA CCCCTGTCAT AATGGAGGCA 
     3301 CCTGCGTGGT CAATGGAGAC TCCTTCACCT GTGTCTGCAA AGAAGGCTGG GAGGGGCCGA 
     3361 TTTGTACTCA AAATACCAAC GACTGCAGTC CTCACCCTTG TTACAACAGC GGAACCTGTG 
     3421 TGGATGGAGA TAACTGGTAC CGGTGCGAAT GTGCCCCTGG TTTTGCTGGG CCAGACTGCA 
     3481 GGATAAACAT CAATGAGTGT CAGTCTTCCC CTTGTGCCTT TGGGGCCACC TGTGTGGATG 
     3541 AGATCAATGG CTACCAGTGT ATCTGCCCTC CAGGACATAG TGGTGCCAAG TGCCATGAAG 
     3601 TTTCAGGGAG ATCTTGCATC ACCATGGGGA GAGTGATACT TGATGGGGCC AAGTGGGATG 
     3661 ACGACTGTAA CACCTGCCAG TGCCTGAATG GACGGGTGGC CTGCTCCAAG GTCTGGTGTG 
     3721 GCCCGAGACC TTGCCGGCTC CACAAAGGGC ACGGCGAGTG TCCCAATGGG CAGAGCTGCA 
     3781 TCCCAGTCCT GGATGACCAG TGTTTCGTGC GCCCCTGCAC TGGTGCTGGC GAGTGTCGGT 
     3841 CCTCCAGCCT CCAGCCAGTG AAGACCAAGT GTACATCGGA CTCTTATTAC CAGGACAATT 
     3901 GTGCGAACAT CACTTTCACC TTTAACAAGG AGATGATGTC TCCAGGTCTC ACCACTGAGC 
     3961 ACATTTGCAG CGAATTGAGG AATCTGAATA TTCTGAAGAA TGTTTCTGCT GAGTATTCAA 
     4021 TCTACATAGC CTGTGAGCCT TCCTTATCAG CAAACAATGA AATACACGTG GCCATTTCTG 
     4081 CCGAAGACAT CCGGGATGAT GGAAACCCTG TCAAGGAAAT TACTGATAAA ATAATAGATC 
     4141 TCGTTAGTAA ACGGGATGGG AACAGCTCAC TTATCGCTGC TGTTGCAGAA GTCAGAGTTC 
     4201 AGAGGCGCCC TCTGAAAAAC AGAACAGATT TCCTGGTTCC TCTGCTGAGC TCTGTCTTAA 
     4261 CAGTGGCTTG GGTCTGTTGC TTGGTGACAG CCTTCTACTG GTGTGTGCGG AAGCGGCGGC 
     4321 GGAAGCCCAG CAGCCACACC CACTCTGCCC CTGAGGACAA CACCACCAAC AATGTGCGCG 
     4381 AACAGCTGAA CCAAATCAAA AACCCCATCG AGAAACACGG AGCCAACACA GTCCCCATCA 
     4441 AGGATTATGA GAACAAAAAC TCCAAAATGT CAAAAATCAG GACACACAAC TCGGAAGTGG 
     4501 AAGAGGATGA TATGGATAAG CACCAGCAGA AAGTCCGGTT TGCCAAACAG CCCGTGTACA 
     4561 CGCTGGTAGA CAGAGAGGAG AAGGTCCCCC AGCGCACGCC GACAAAACAC CCGAACTGGA 
     4621 CAAACAAACA GGACAACAGA GACTTGGAAA GTGCCCAGAG CTTGAACCGG ATGGAGTACA 
     4681 TCGTAACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4741 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4801 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4861 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4921 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4981 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     5041 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     5101 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     5161 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5221 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5281 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5341 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5401 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5461 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5521 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5581 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5641 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5701 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5761 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5821 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5881 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5941 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     6001 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     6061 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6121 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6181 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6241 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6301 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6361 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6421 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6481 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6541 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6601 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6661 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6721 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6781 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6841 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6901 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6961 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     7021 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     7081 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7141 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7201 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7261 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7321 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7381 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7441 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7501 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7561 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7621 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7681 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7741 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7801 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7861 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7921 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7981 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     8041 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     8101 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     8161 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8221 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8281 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8341 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8401 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8461 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8521 AAATTTAACG CGAATTTT
//