LOCUS       RR203904                8343 bp    DNA     circular  SYN 
DEFINITION  Itgam (Myc-DDK-tagged ORF) - Rat integrin alpha M (Itgam), (10 ug)
ACCESSION   NM_012711
VERSION     NM_012711.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4498..4589 
                     /label="Myc-DDK"  
     misc_feature    4619..5204 
                     /label="PolyA signal"  
     misc_feature    4676..4695 
                     /label="Restriction Enzyme"  
     misc_feature    5480..6152 
                     /label="ColE1"  
     misc_feature    6582..7376 
                     /label="Kan/Neo"  
     misc_feature    7411..7713 
                     /label="SV40 ori"  
     misc_feature    7799..8276 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4473..4480 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4473 
                     /label="Itgam"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACTCTTAAA GTTCTTCTGG CGACAGTCCT AACCTTGTGT CATGGCTTCA 
     1081 ATTTGGACAC TGAAAATCCC ATGACCTTCC AAGAGAATGC AAGTGGCTTC GGGCAGAGTG 
     1141 TGATCCAGCT TGGTGAAACC CGAGTGGTTG TTGCAGCCCC CCAGGAGGTA AAGGCGGTTA 
     1201 ACCAGACAGG TGCCCTCTAC CAGTGTGACT ACAGCACAAA CCGGTGCGAC CCCATTCCCC 
     1261 TGCAAGTACC TCCAGAGGCT GTGAATATGT CCTTGGGCCT GTCCCTGGCT GCTACTACCG 
     1321 TCCCTCCACA GCTGCTGGCT TGTGGCCCCA CAGTACACCA GAACTGCAAG GAGAACACTT 
     1381 ACGTCAATGG ATTGTGCTAC CTATTCGGCT CCAACCTGCT GAGGAAGCCC CAGCAGTTCC 
     1441 CAGAGGCTCT CAGAGGATGT CCTCAGCAGG AGAGCAACAT TGCCTTCTTG ATTGACGGCT 
     1501 CCGGTAGCAT CAACACGATC GACTTTCAGA AGATGAAGGA ATTTGTCTCA ACTGTGATGG 
     1561 ACCAGTTCCA AAAGTCTAAA ACCTTGTTCT CTTTGATGCA GTACTCTGAT GAATTCCGGA 
     1621 CTCACTTCAC CTTCAATGAT TTCAAGAGAA ACCCTGACCC AAAATCACAT GTGAGACCCA 
     1681 TAAGGCAGCT GAATGGAAGG ACAAAAACTG CCTCAGGGAT CCGTAAAGTA GTGAGAGAAC 
     1741 TGTTTCAGAA AATCAATGGG GCCCGGGACA ATGCCGCGAA GATCCTAGTT GTCATCACAG 
     1801 ACGGAGAAAA GTTTGGTGAC CCCCTAAATT ATGAGGATGT CATTCCTGAG GCAGAGGAAG 
     1861 CAGGGATCAT TCGCTACGTT ATTGGGGTGG GAAACGCCTT CCACAAACCA CAGTCCCGCA 
     1921 GAGAGCTTGA CACCATCGCG TCTAAGCCAG CTGGTGATCA CGTGTTCCAA GTGGACAACT 
     1981 TTGAAGCTCT GAATACCATT CGGAACCAGC TCCAGGAAAA GATCTTTGCA ATTGAGGGCA 
     2041 CCCAGACAGG AAGTACCAGC TCCTTTGAGC ATGAGATGTC TCAAGAAGGC TTTAGCGCTG 
     2101 CCATTACCTC CAACGGTCCC TTGCTGGGCT CCGTGGGGAG CTTTGACTGG GCAGGTGGAG 
     2161 CCTTCCTGTA TCCATCAAAG GATAAAGCCA GTTTCATCAA CACAACCAGA ATAGATTCAG 
     2221 ACATGAACGA TGCTTACTTG GGTTATGCTT CTGCAGTCAT CTCGAGGAAC CGCGTCCAAA 
     2281 GCTTGGTTTT AGGGGCACCT CGATATCAGC ATATCGGCCT GGTGGTGATG TTCAAGCAGA 
     2341 ATTTCGGTGC CTGGGAGCCC CACACTGATA TCAAGGGCAG CCAGATCGGC TCTTATTTTG 
     2401 GGGCCTCCCT CTGTTCTGTG GACATGGACG CTGATGGCAA TACCAACTTG ATCCTCATTG 
     2461 GGGCCCCTCA TCACTATGAG AAGACCCGAG GGGGCCAGGT GTCAGTGTGC CCCTTGCCTA 
     2521 GGGGGAGAGC ACGGTGGCAG TGTGAAGCTA TTCTCCATGG CGATCAGGGC CATCCATGGG 
     2581 GTCGCTTTGG GGCAGCATTG ACAGTGCTGG GAGATGTGAA TGGAGACAAA CTGACAGATG 
     2641 TGGCCATTGG GGCCCCAGGA GAGCAGGAGA ATCAGGGTGC TGTCTACATT TTTCATGGAG 
     2701 CATCAGTAGC CAGCATCAGT ACCCCTCACA GCCAGCGGAT CGCAGGCGCC CGCTTCTCCC 
     2761 CCGGGCTCCA GTACTTTGGG CAGTCTCTGA GTGGGGGCAA GGATCTCACA ATGGACGGCC 
     2821 TGATGGACTT GGCTGTGGGG GCGCAGGGGC GTCTTCTGCT GATCAGAGCC CAGCCTGTGC 
     2881 TGAGACTGGA GGCAACCATG GAATTCAGCC CCAAGAATCT AGCAAGGAGT GTGTTTGCGT 
     2941 GTCAAGAAGA AGTAGTCAAA AACAAGGATG CTGGGGAGGT CAGAGTCTGC CTCCAAGTCC 
     3001 GCAAGAACAC CAAGGACAGG CTGCGAGAAG GAGACATCCA GAGCACTGTC ACTTATGACC 
     3061 TGGCTTTAGA CCCCGGCCGC TCAGTTGTCC GAGCCTTCTT TGATGAAACA AAGAACGGCA 
     3121 TACTCAGGCG CATCCGGGTC TTTGGATTGA CACAGAAATG TGAAACCCTG AAGCTAATTT 
     3181 TACCGGACTG TGTGGACAAC TCAGTGAGTC CCATCATCCT GCGCCTTAAT TACACACTGG 
     3241 TTGGGGAGCC CTTGCGGTCC TCTAGGGACC TTCGGCCAGT TCTGGCTATG GAGGCTCAGA 
     3301 GGATCTTCAC AGCTATGTTT CCCTTTGAGA AGAATTGTGG CAATGACACC ATTTGCCAGG 
     3361 ATGATCTCAG CATCACCGTG AGTTCCACAC GCGTGAACAC TTTGGTGGTG GGAGACTCCC 
     3421 GGGACTTTGA CGTGAGTGTG ACTCTGAGAA ATGATGGCGA GGATTCCTAT GGGACCAAGG 
     3481 TTACCTGCTA TTACCCATCT GGCTTGTCTT ACCGGAAGGT GTCAGCAAGC CAGAACCAGT 
     3541 TCTCCAAGAA GCCTTGGCGT GTGATAGCTG AGCCCAGTTC TTCTGAAGGG CAAGGGGTTC 
     3601 TGAAGAGCAC CATCTGGGAC ATAAACCATC CCATCTTTCC CGCTAATTCT GAGGTCACGT 
     3661 TTAATGTCAC ATTTGATGTG GACTCTGATG CCTCCCTTGG GAACAAACTG CTCCTCAAGG 
     3721 TCGTTGTGAC CAGTGAGAAC AACGTGTCCA GGACCGACAA AACTGAGTCT CAGTTGGAGT 
     3781 TGCCTGTGAA GTACGCCATC TACATGGTCG TCACCAGTGG TGAGAGTTCT ATCAAATATC 
     3841 TCAACTTCAC AGCTTCAGAG ATGACCAGTA AGGTCATACA GCATCAGTAC CAGTTCAACA 
     3901 ACCTGGGCCA GAGGAGCTTC CCTGTCAGCG TGGTCTTCTG GATCCCTGTC CAGATCAACA 
     3961 AGGTGACCAT ATGGGATCCT CCCCAGGTCA CCTTCTCCCA GAACCTCTCA AGTGTCTGTC 
     4021 GCACTGAGCA GAAATCCCCC TCTCACTCCA AGTTCCAGGA TGAGCTTGAA AGGACCCCAG 
     4081 TGCTGAACTG CTCTGTTGCA GTCTGCAAGA GAATCCAGTG TGACATCCCT TCCTTCAACA 
     4141 GTAAAGAAAT ATTCAACGTC ACCCTCCAGG GCAATCTGCT ATTTGACTGG TACATCGAGA 
     4201 CTTCTCATGA CCACCTCCTG CTTGTGAGCA CTGCCGAGAT CCTCTTTAAC GACTCCGCAT 
     4261 TTGCCCTACT TCCGGGGCAG GAGACGTTTG TGAAGGCTCA GACAGAGACC AAAGTGGAGC 
     4321 CATATACAGT TCACAATCCT GTACCACTCA TTGTGGGCAG CTCCGTCGGA GGCCTGGTGC 
     4381 TCTTGGCTCT CATCACTGCT GGCCTATACA AGCTTGGCTT TTTCAAACGA CAGTACAAGG 
     4441 ACATGATGAA TGAAGCCGGT GGCCAAGATG GCCCACCTCA GAGCGGACCG ACGCGTACGC 
     4501 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4561 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4621 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4681 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4741 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4801 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4861 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4921 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4981 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5041 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5101 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5161 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5221 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5281 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5341 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5401 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5461 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5521 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5581 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5641 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5701 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5761 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5821 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     5881 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     5941 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6001 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6061 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6121 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6181 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6241 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6301 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6361 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6421 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6481 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6541 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6601 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6661 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6721 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6781 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6841 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6901 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     6961 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7021 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7081 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7141 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7201 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7261 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7321 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7381 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7441 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7501 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7561 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7621 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7681 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7741 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7801 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7861 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7921 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     7981 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     8041 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8101 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8161 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8221 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8281 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8341 TTT
//