LOCUS       RR203879                6927 bp    DNA     circular  SYN 
DEFINITION  Plk2 (Myc-DDK-tagged ORF) - Rat polo-like kinase 2 (Drosophila) (Plk2), (10 ug)
ACCESSION   NM_031821
VERSION     NM_031821.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3084..3175 
                     /label="Myc-DDK"  
     misc_feature    3205..3790 
                     /label="PolyA signal"  
     misc_feature    3262..3281 
                     /label="Restriction Enzyme"  
     misc_feature    4066..4738 
                     /label="ColE1"  
     misc_feature    5168..5962 
                     /label="Kan/Neo"  
     misc_feature    5997..6299 
                     /label="SV40 ori"  
     misc_feature    6385..6862 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3066..3072 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3066 
                     /label="Plk2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCTCCTG CGGACTATCA CCTACCAGCC GGCCGCCGGC ACCAAGATGT 
     1081 GCGAGCAGGC GCTGGGCAAA GCTTGCGGCG GGGACTCAAA GAAGAAGCGA CCGCAGCAGC 
     1141 CTTCTGAAGA CGGGCAGTCC CAAGCCCAGG TGACCCCGGC GGCCCCACAC CACCATCATC 
     1201 ACCATTCTCA CTCCGGACCC GAGATCTCGC GGATTATAGT CGACCCCACG ACGGGGAAGC 
     1261 GCTACTGCCG GGGCAAAGTG CTGGGCAAGG GTGGATTTGC AAAGTGTTAC GAAATGACAG 
     1321 ATCTGACAAA CAACAAAGTC TACGCTGCAA AAATTATCCC TCACAGCAGA GTAGCTAAAC 
     1381 CTCATCAAAG GGAAAAGATC GACAAAGAAA TTGAGCTTCA CAGAATCCTG CACCATAAGC 
     1441 ATGTAGTGCA GTTTTACCAC TACTTTGAAG ACAAAGAAAA CATTTACATC CTCTTGGAGT 
     1501 ACTGCAGTAG AAGGTCCATG GCTCATATCT TGAAAGCAAG AAAGGTGTTG ACAGAGCCAG 
     1561 AAGTCCGATA CTACCTCAGG CAGATTGTGT CGGGACTCAA GTATCTTCAC GAACAAGAAA 
     1621 TCTTGCATAG GGATCTCAAG CTAGGGAACT TTTTTATTAA TGAGGCCATG GAGCTGAAGG 
     1681 TTGGAGACTT CGGCTTGGCT GCCAGACTGG AACCTTTGGA ACACAGAAGG AGAACAATAT 
     1741 GTGGAACCCC AAATTATCTC TCCCCTGAAG TCCTCAACAA ACAAGGACAT GGCTGTGAAT 
     1801 CAGACATCTG GGCCTTAGGC TGTGTAATGT ATACAATGCT GCTAGGAAGA CCTCCATTCG 
     1861 AAACAACAAA TCTGAAAGAA ACGTACAGGT GCATAAGAGA AGCAAGATAT ACAATGCCGT 
     1921 CTTCATTACT GGCCCCCGCC AAGCACTTGA TAGCCAGCAT GCTGTCCAAA AACCCAGAGG 
     1981 ACCGCCCCAG TTTGGATGAC ATCATTCGGC ATGACTTCTT TCTGCAGGGC TTCACTCCAG 
     2041 ACAGACTCTC TTCTAGCTGT TGCCACACAG TTCCAGACTT CCACTTGTCA AGCCCAGCTA 
     2101 AGAATTTCTT TAAGAAAGCT GCTGCCGCTC TTTTTGGTGG CAAAAAGGAC AAAGCAAGAT 
     2161 ATAATGACAC ACACAATAAA GTGTCTAAGG AAGATGAAGA CATCTACAAG CTTCGGCATG 
     2221 ATTTGAAGAA AACATCGATA ACCCAGCAAC CCAGCAAACA CAGAACAGAT GAGGAGCTCC 
     2281 AGCCTCCTCC AACTACCTTT GCCAAATCTG GGACATCCGC AGTGGAAAAC AAACAGCAGA 
     2341 TTGGAGATGC AATCCGGATG ATAGTTAGAG GGACGCTGGG CAGCTGCAGC AGCAGCAGTG 
     2401 AATGCCTTGA GGATAGCACC ATGGGAAGTG TTGCAGACAC AGTGGCAAGA GTCCTTCGAG 
     2461 GGTGTCTAGA AAACATGCCT GAAGCTGACT GTATTCCCAA AGAGCAGTTG AGCACGTCCT 
     2521 TTCAGTGGGT CACCAAATGG GTCGACTACT CCAACAAATA CGGCTTTGGA TACCAGCTCT 
     2581 CAGACCACAC TGTTGGAGTC CTTTTCAACA ACGGCGCTCA CATGAGCCTC CTTCCAGACA 
     2641 AAAAGACAGT TCACTATTAT GCGGAACTTG GCCAATGCTC TGTTTTCCCA GCAACAGATG 
     2701 CCCCCGAACA ATTTATTAGT CAAGTGACGG TGCTGAAATA CTTTTCCCAC TACATGGAGG 
     2761 AGAACCTCAT GGATGGTGGC GATCTCCCTA GTGTTACTGA CATTCGAAGA CCTCGGCTCT 
     2821 ACCTCCTTCA GTGGTTAAAG TCTGATAAAG CCTTAATGAT GCTCTTCAAT GACGGCACAT 
     2881 TTCAGGTGAA TTTCTACCAC GATCATACAA AGATAATTAT CTGTAACCAA AATGAAGAAT 
     2941 ACCTTCTCAC ATACATCAAC GAAGACAGGA TATCTACAAC TTTCAGACTG ACGACCCTGT 
     3001 TGATGTCTGG CTGTTCGCTA GAATTGAAAC ATCGAATGGA ATACGCCCTG AACATGCTCT 
     3061 TGCAGAGATG TAACACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     3121 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     3181 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     3241 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     3301 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     3361 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     3421 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     3481 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     3541 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     3601 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     3661 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     3721 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     3781 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     3841 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     3901 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     3961 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     4021 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     4081 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     4141 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     4201 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     4261 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     4321 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     4381 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     4441 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     4501 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     4561 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     4621 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     4681 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     4741 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     4801 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     4861 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     4921 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     4981 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     5041 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     5101 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     5161 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     5221 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     5281 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     5341 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     5401 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     5461 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     5521 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     5581 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     5641 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     5701 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     5761 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     5821 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     5881 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     5941 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     6001 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     6061 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     6121 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     6181 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     6241 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     6301 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     6361 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     6421 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     6481 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     6541 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     6601 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     6661 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     6721 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     6781 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     6841 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     6901 ATTTAACAAA AATTTAACGC GAATTTT
//