LOCUS       RR203867                7109 bp    DNA     circular  SYN 
DEFINITION  Lss (Myc-DDK-tagged ORF) - Rat lanosterol synthase (2,3-oxidosqualene-lanosterol cyclase) (Lss), (10 ug)
ACCESSION   NM_031049
VERSION     NM_031049.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3266..3357 
                     /label="Myc-DDK"  
     misc_feature    3387..3972 
                     /label="PolyA signal"  
     misc_feature    3444..3463 
                     /label="Restriction Enzyme"  
     misc_feature    4248..4920 
                     /label="ColE1"  
     misc_feature    5350..6144 
                     /label="Kan/Neo"  
     misc_feature    6179..6481 
                     /label="SV40 ori"  
     misc_feature    6567..7044 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3248..3254 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3248 
                     /label="Lss"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACCGAGGGC ACGTGTCTGC GGCGTCGTGG GGGACCCTAT AAAACTGAGC 
     1081 CCGCCACCGA TCTCACCCGC TGGCGGCTCC ATAATGAGTT GGGTCGGCAG AGATGGACTT 
     1141 ATTATCAAGC GGAGGAGGAC CCTGGTCGAG AACAGACGGG GCTAGAGGCC CACTCTTTGG 
     1201 GACTGGACAC AACAAGTTAT TTCAAGAACT TACCTAAAGC TCAAACAGCC CATGAGGGGG 
     1261 CCCTGAACGG AGTAACCTTT TATGCCAAGC TGCAGGCTGA GGATGGACAC TGGGCTGGTG 
     1321 ATTATGGTGG TCCACTCTTC CTCTTGCCAG GTCTCCTGAT TACGTGTCAC ATAGCACACA 
     1381 TCCCCCTGCC GGCTGGATAC AGAGAGGAAA TGGTACGGTA CTTGCGCTCA GTGCAGCTTC 
     1441 CCAATGGCGG CTGGGGCTTG CACATTGAGG ACAAGTCCAC GGTGTTTGGG ACTGCCCTGA 
     1501 GCTATGTGTC TCTCAGAATC CTGGGTATTG GACCTGATGA TCCTGACCTT GTGCGTGCTC 
     1561 GGAACATTCT TCACAAAAAA GGTGGTGCAG TGGCCATCCC TTCCTGGGGG AAGTTCTGGC 
     1621 TGGCTGTCCT GAATGTTTAC AGCTGGGAAG GAATCAATAC CCTCTTCCCT GAGATGTGGC 
     1681 TGCTTCCTGA ATGGTTTCCT GCACATCCCT CCACTCTGTG GTGTCACTGC CGGCAGGTCT 
     1741 ATCTGCCCAT GAGCTACTGC TACGCCACTC GGCTAAGTGC CTCAGAGGAC CCACTGGTTC 
     1801 AGAGCCTCCG CCAGGAACTC TATGTGGAGG ATTATGCCAG CATCGATTGG CCAGCACAGA 
     1861 AGAACAACGT GTGCCCCGAT GACATGTACA CGCCACACAG CTGGCTGCTG CACGTGGTAT 
     1921 ATGGACTCCT CAACCTGTAT GAACGTTTCC ACAGTACCAG CCTGCGGAAG TGGGCCATCC 
     1981 AGTTGCTGTA TGAACATGTC GCAGCTGATG ATCGGTTCAC GAAATGCATC AGCATTGGCC 
     2041 CGATCTCAAA AACTGTCAAC ATGCTTATTC GTTGGTCAGT GGACGGACCA TCCTCCCCTG 
     2101 CCTTCCAGGA GCACGTCTCG AGGATCAAAG ATTATCTTTG GCTGGGCCTT GACGGCATGA 
     2161 AAATGCAGGG CACCAATGGA TCACAGACCT GGGACACTTC ATTTGCTGTC CAAGCCCTGC 
     2221 TGGAGGCAGG TGCACACCGA AGACCTGAGT TTTTGCCCTG CCTGCAGAAG GCTCACGAGT 
     2281 TCCTGCGGCT TTCCCAGGTC CCAGACAACA ATCCTGACTA CCAGAAGTAT TATCGCCATA 
     2341 TGCACAAGGG TGGTTTCCCC TTCAGCACAC TGGACTGTGG CTGGATCGTT GCTGACTGCA 
     2401 CGGCCGAGGC TTTGAAGGCT GTGCTGCTCC TGCAGGAGCG GTGTCCCTCA ATCACCGAGC 
     2461 ATGTCCCCCG AGAGCGACTC TACGATGCTG TGGCTGTGTT GTTGAGCATG AGGAATTCCG 
     2521 ATGGAGGGTT TGCTACCTAT GAGACTAAGC GTGGCGGGTA TTTGCTGGAG CTGCTGAACC 
     2581 CCTCAGAGGT CTTTGGAGAC ATCATGATTG ACTACACGTA TGTAGAGTGT ACCTCCGCAG 
     2641 TGATGCAGGC CCTGAGGCAC TTCCGCGAGT ACTTCCCAGA CCACAGGGCT ACAGAGATCA 
     2701 GGGAGACCCT CAATCAAGGC CTGGACTTCT GCCGAAAGAA GCAGAGAGCG GATGGCTCGT 
     2761 GGGAGGGCTC CTGGGGGGTT TGCTTCACCT ATGGCACCTG GTTTGGCTTG GAAGCATTCG 
     2821 CTTGCATGGG ACATATCTAC CAAAATAGGA CTGCTTGTGC AGAAGTAGCT CAGGCCTGCC 
     2881 ACTTCCTCCT GTCGCGGCAG ATGGCGGATG GGGGCTGGGG GGAGGACTTT GAGTCCTGTG 
     2941 AGCAGCGGCG GTACGTGCAG AGTGCCGGGT CCCAGGTCCA TAGTACGTGC TGGGCCCTGC 
     3001 TGGGTTTGAT GGCTGTCAGG CATCCCGACA TCTCTGCTCA GGAGAGAGGA ATCAGATGTC 
     3061 TGCTAGGGAA ACAGCTCCCC AACGGAGACT GGCCTCAGGA GAACATCTCT GGGGTCTTCA 
     3121 ACAAGTCCTG TGCCATCAGC TACACAAATT ACAGAAACAT CTTCCCCATC TGGGCCCTGG 
     3181 GCCGCTTCTC CAGCCTGTAT CCTGACAATA CCCTTGCTGG CCACATTATC CTGACAATAC 
     3241 CCTTGCTGGC CACATTACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA 
     3301 TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG 
     3361 CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG 
     3421 TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT 
     3481 TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG 
     3541 TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG 
     3601 AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC 
     3661 AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC 
     3721 TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT 
     3781 CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG 
     3841 CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG 
     3901 ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT 
     3961 TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA 
     4021 CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA 
     4081 TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT 
     4141 AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA 
     4201 AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT 
     4261 CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG 
     4321 TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC 
     4381 AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC 
     4441 GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA 
     4501 TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT 
     4561 ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC 
     4621 TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA 
     4681 CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA 
     4741 AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA 
     4801 AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT 
     4861 TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG 
     4921 GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG 
     4981 TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA 
     5041 TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG 
     5101 TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC 
     5161 TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG 
     5221 CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG 
     5281 AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT 
     5341 TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT 
     5401 GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA 
     5461 GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA 
     5521 GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC 
     5581 AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG 
     5641 CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA 
     5701 GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG 
     5761 GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT 
     5821 TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA 
     5881 GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG 
     5941 TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT 
     6001 CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG 
     6061 AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG 
     6121 GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT 
     6181 GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC 
     6241 TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC 
     6301 GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT 
     6361 ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG 
     6421 GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT 
     6481 GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG 
     6541 CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA 
     6601 GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC 
     6661 CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG 
     6721 TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT 
     6781 TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC 
     6841 CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG 
     6901 ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT 
     6961 CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG 
     7021 TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC 
     7081 TGATTTAACA AAAATTTAAC GCGAATTTT
//