LOCUS       RR203833                8694 bp    DNA     circular  SYN 
DEFINITION  Upf2 (Myc-DDK-tagged ORF) - Rat UPF2 regulator of nonsense transcripts homolog (yeast) (Upf2), (10 ug)
ACCESSION   NM_001108421
VERSION     NM_001108421.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4849..4940 
                     /label="Myc-DDK"  
     misc_feature    4970..5555 
                     /label="PolyA signal"  
     misc_feature    5027..5046 
                     /label="Restriction Enzyme"  
     misc_feature    5831..6503 
                     /label="ColE1"  
     misc_feature    6933..7727 
                     /label="Kan/Neo"  
     misc_feature    7762..8064 
                     /label="SV40 ori"  
     misc_feature    8150..8627 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4824..4831 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4824 
                     /label="Upf2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCAGCTGAG CGGAAAAAGT CAGCAAGTAT GGAGGAAAAA GAATCTTTAC 
     1081 TAAACAACAA GGAAAAAGAC TGCAGTGAGA GGCGGCCAGT GAGCAGCAAG GAGAAATCAA 
     1141 AAGACGATCT CAAGATCACT GCCAAGAAGG AGGTCAGTAA GGTCCCAGAA GACAAAAAGA 
     1201 AGAAACTGGA AGAAGATAAG AGAAAAAAGG AAGACAAGGA ACGTAAGAAA AAAGAGGAAG 
     1261 AGAAGGTGAA GGCAGAGGAA GAGTTGAAGA AGAAGGAAGA GGAAGAAAAG AAAAAACAGG 
     1321 AAGAGGAAGA GAGGAAAAAG CAGGAGGAGC AGGCCAAGCG GCAGCAGGAA GAAGCAGCTG 
     1381 CCCAGCTGAA AGAGAAAGAA GAATCTCTTC AGCTTCATCA GGAGGCGTGG GAACGCCATC 
     1441 AACTACGAAA GGAACTCCGT AGCAAAAACC AAAATGCTCC AGACAACCGA CCAGAGGAAA 
     1501 ATTTCTTTAG CCGCCTAGAT TCGAGCTTAA AGAAAAATAC TGCTTTTGTC AAGAAACTAA 
     1561 AAACAATTAC AGAACAACAG AGAGACTCCT TGTCTCATGA TTTTAATGGC CTGAATTTAA 
     1621 GCAAATATAT TGGGGAAGCC GTAGCTTCCA TTGTGGAAGC AAAATTAAAA ATCTCTGACG 
     1681 TGAACTGTGC CGTGCACCTC TGCTCTCTCT TTCACCAGCG GTACGCTGAC TTTGCACCCT 
     1741 CACTTCTTCA GGTGTGGAAA AAACATTTTG AAGCAAGGAA AGAGGAGAAA ACGCCCAACA 
     1801 TTACCAAGCT AAGAACTGAC TTGCGTTTCA TTGCAGAATT GACAATAGTG GGAATCTTCA 
     1861 CTGACAAGGA AGGTCTTTCC TTAATCTATG AACAGCTAAA AAGTATTATT AATGCTGATC 
     1921 GAGAATCCCA CACTCATGTG TCTGTGGTGA TTAGTTTCTG TCGTCATTGT GGAGATGATA 
     1981 TTGCTGGACT TGTACCGAGG AAAGTAAAGA GTGCTGCAGA GAAGTTTAAT TTGAACTTTC 
     2041 CTCCTAGTGA GATAATTAGC CCAGAGAAGC AGCAGCCTTT CCAGAATCTT TTAAAAGAGT 
     2101 ACTTTACGTC TCTGACCAAA CACCTGAAAA GGGACCACAG GGAGCTTCAG AATACTGAGA 
     2161 GGCAAAACAG GCGTATTCTG CACTCTAAAG GCGAGCTAAG TGAAGACAGG CATAAACAGT 
     2221 ATGAAGAGTT CGCCATGTCT TACCAGAAGC TGCTGGCAAA TTCTCAATCC TTAGCCGACC 
     2281 TTTTGGATGA AAATATGCCA GATCTTCCTC AGGACAAACC GACACCAGAA GAGCATGGAC 
     2341 CTGGAATCGA TATATTTACA CCTGGAAAAC CTGGAGAATA TGACTTAGAA GGTGGCATAT 
     2401 GGGAAGACGA AGACGCTCGG AATTTTTATG AAAACCTCAT CGATTTGAAA GCATTTGTTC 
     2461 CAGCCATCTT GTTTAAAGAC AATGAAAAAA GTCAGAACAA AGATTCTAGC AAAGATGATT 
     2521 CCAAAGAGGC AAAAGAACCA AAGGATAATA AGGAAGCATC AAGTCCCGAT GATTTGGAAC 
     2581 TTGAGTTGGA GAATTTAGAA ATTAATGATG ACACACTGGA GTTAGAAGGT GGAGATGAAG 
     2641 CTGAAGATCT TACAAAGAAA CTCCTTGATG AACAAGAACA AGAAGATGAA GAAGCCAGCA 
     2701 CTGGATCTCA CCTCAAACTC ATAGTGGATG CTTTCCTCCA GCAGCTGCCC AACTGTGTCA 
     2761 ACCGAGACCT GATTGACAAG GCAGCCATGG ACTTTTGCAT GAACATGAAC ACAAAGGCAA 
     2821 ACAGGAAGAA GTTGGTTCGG GCGCTCTTCA TAGTTCCTAG GCAAAGGTTG GATTTGCTCC 
     2881 CATTTTATGC AAGATTGGTT GCTACATTGC ATCCCTGCAT GTCTGATGTA GCAGAGGATC 
     2941 TTTGTTCCAT GCTGAGGGGG GATTTCAGAT TTCATGTTCG AAAAAAGGAC CAGATCAACA 
     3001 TTGAAACAAA GAATAAAACT GTCCGTTTTA TTGGAGAACT AACCAAGTTT AAGATGTTCA 
     3061 CCAAAAATGA TACACTGCAT TGTCTAAAGA TGCTTCTGTC AGACTTTTCT CATCATCATA 
     3121 TTGAAATGGC ATGTACCCTA CTGGAGACCT GTGGACGGTT TCTTTTCCGA TCTCCAGAAT 
     3181 CACACCTGAG GACCAGTGTG CTTTTGGAGC AAATGATGAG AAAGAAGCAA GCAATGCACC 
     3241 TGGACGCGCG CTACGTTACA ATGGTGGAGA ACGCGTATTA CTACTGCAAC CCACCACCAG 
     3301 CCGAGAAGAC AGTGAGAAGG AAGCGGCCTC CTCTTCAGGA GTATGTCCGC AAACTTCTGT 
     3361 ACAAGGATCT CTCTAAGGTC ACCACGGAAA AGGTTTTGAG ACAGATGCGA AAGTTGCCCT 
     3421 GGCAGGACCA AGAAGTTAAA GACTATGTCA TTTGTTGTAT GATAAACATT TGGAATGTGA 
     3481 AATACAATAG TATTCACTGT GTAGCCAACC TCCTAGCAGG ACTAGTGCTT TACCAGGAGG 
     3541 ATGTTGGGAT CCACGTTGTG GACGGAGTAC TTGAAGATAT TCGATTGGGA ATGGAGGTTA 
     3601 ATCAACCTAA ATTTAACCAG AGGCGCATCA GTAGTGCCAA GTTCTTAGGA GAACTTTATA 
     3661 ATTACAGAAT GGTCGAATCG GCTGTTATTT TCAGAACTCT TTATTCTTTT ACTTCATTTG 
     3721 GTGTCAACCC TGACGGGTCT CCAAGTTCCC TGGACCCTCC TGAGCATCTG TTTAGAATTA 
     3781 GACTAGTCTG CACTATTTTG GATACCTGTG GCCAGTACTT TGACAGAGGT TCCAGTAAAA 
     3841 GAAAACTTGA CTGCTTCCTT GTATATTTTC AGCGTTATGT TTGGTGGAAG AAAAGTTTGG 
     3901 AGGTTTGGAC AAAAGACCAT CCATTTCCTA TTGATATAGA TTACATGATC AGTGATACAC 
     3961 TAGAACTGCT AAGACCAAAG ATCAAACTCT GTAATTCTCT GGAAGAATCC ATCAGGCAGG 
     4021 TACAAGACTT GGAACGAGAA TTCTTAATAA AACTAGGCCT AGTAAATGAC AAAGAGTCCA 
     4081 AAGATTCTAT GACAGAAGGG GAAAATCTTG AAGAGGATGA AGAAGAAGAA GAAGGAGGAG 
     4141 CTGAGACAGA GGAACAGTCT GGAAATGAGA GTGAAGTAAA CGAGCCAGAA GAAGAAGAGG 
     4201 GTTCTGATGA TGAAGACGAG GGAGAGGAGG AGGAAGAAGA GAATACAGAT TACCTCACAG 
     4261 ATTCCAACAA GGAGAACGAG ACTGATGAAG AGAATGCTGA GGTGATGATT AAGGGAGGCG 
     4321 GACTGAAGCA TGTTCCTTGT GTAGAAGATG AGGACTTCAT TCAAGCTCTG GACAAAATGA 
     4381 TGCTAGAAAA CCTTCAGCGA AGTGGTGAAT CTGTGAAAGT ACACCAACTT GATGTTGCTA 
     4441 TTCCTTTGCA TCTCAAAAGC CAGCTGAGGA AAGGGCCCCC ACTTGGTGGT GGGGAAGGAG 
     4501 AGACAGAGTC TGCAGACACG ATGCCGTTTG TCATGCTGAC AAGGAAAGGC AATAAACAGC 
     4561 AGTTTAAGAT TCTCAATGTG CCCATGTCAT CCCAACTTGC TGCAAATCAC TGGAACCAGC 
     4621 AACAAGCAGA ACAAGAGGAG AGGATGAGGA TGAAAAAGCT CACCTTAGAC ATCAACGAAC 
     4681 GACAAGAACA AGAAGATTAT CAAGAAATGT TGCAGTCTCT CGCACAGCGC CCAGCTCCAG 
     4741 CGAACACTAA TCGTGAGCGG CGGCCTCGTT ACCAACATCC AAAGGGAGCG CCTAATGCAG 
     4801 ATCTAATCTT TAAGACTGGT GGGAGGAGAC GTAGCGGACC GACGCGTACG CGGCCGCTCG 
     4861 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4921 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4981 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     5041 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     5101 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5161 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5221 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5281 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5341 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5401 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5461 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5521 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5581 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5641 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5701 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5761 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5821 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5881 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5941 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     6001 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     6061 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6121 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6181 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6241 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6301 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6361 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6421 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6481 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6541 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6601 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6661 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6721 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6781 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6841 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6901 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6961 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     7021 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     7081 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7141 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7201 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7261 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7321 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7381 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7441 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7501 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7561 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7621 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7681 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7741 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7801 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7861 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7921 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7981 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     8041 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     8101 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8161 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8221 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8281 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8341 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8401 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8461 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8521 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8581 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8641 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//