LOCUS       RR203738                8949 bp    DNA     circular  SYN 
DEFINITION  Tnr (Myc-DDK-tagged ORF) - Rat tenascin R (Tnr), (10 ug)
ACCESSION   NM_013045
VERSION     NM_013045.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5106..5197 
                     /label="Myc-DDK"  
     misc_feature    5227..5812 
                     /label="PolyA signal"  
     misc_feature    5284..5303 
                     /label="Restriction Enzyme"  
     misc_feature    6088..6760 
                     /label="ColE1"  
     misc_feature    7190..7984 
                     /label="Kan/Neo"  
     misc_feature    8019..8321 
                     /label="SV40 ori"  
     misc_feature    8407..8884 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5088..5094 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5088 
                     /label="Tnr"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGATCGAA GGGGAAACAG TAGTCTTGAA GAACATGCTC ATTGGTGTAA 
     1081 ACCTGATCCT GTTGGGATCC ATGCTCAAGC CTTCTGAATG CCGGCTGGAA GTGACTACAG 
     1141 AAAGGGTCCA GAGACAAACT GTGGAGGAGG AAGGAGGGGC TTCCAGCTAC AACACATCCA 
     1201 GCAAGGAACA GCCTATGGTC TTTAACCATG TGTATAACAT CAACGTGCCA CTCGAAAGCC 
     1261 TCTGCTCCTC GGGGCTGGAG GCCTCAGCTG AGCAGGACGT GAGTGCCGAA GATGATACTC 
     1321 TGGCAGAATA CACAGGCCAA ACCTCAGACC ATGAAAGCCA GGTCACCTTC ACCCACAAGA 
     1381 TCAACCTCCC CAAAAAAGCC TGCCCATGTG CAAGCTCTGC CCAGGTACTG CAGGAACTGT 
     1441 TGAGCCGAAT CGAGATGCTG GAGAGGGAGG TGTCAGTGCT GCGAGACCAG TGCAACACCA 
     1501 ACTGCTGTCA GGAAAGTGCT GCCACAGGAC AACTGGACTA TGTCCCTCAC TGCAGCGGCC 
     1561 ATGGCAACTT TAGCTTCGAG TCCTGTGGCT GCATCTGCAA TGAAGGCTGG TTTGGCAAGA 
     1621 ACTGCTCAGA GCCCTACTGC CCATTGGGCT GCTCCAGTCG GGGTGTATGT GTCGATGGCC 
     1681 AGTGCATTTG TGACAGTGAA TACAGCGGAG ATGACTGTTC AGAGCTCCGG TGCCCAACAG 
     1741 ACTGCAGTTC CCGAGGGCTC TGTGTGGATG GGGAATGTGT CTGTGAAGAG CCCTACACAG 
     1801 GCGAGGACTG CAGGGAGCTG CGTTGCCCTG GGGACTGTTC AGGGAAGGGG CAATGTGCCA 
     1861 ATGGTACCTG CCTGTGCCAA GAGGGCTATG CTGGTGAGGA CTGCAGTCAG CGACGGTGTC 
     1921 TGAATGCTTG CAGTGGGCGA GGTCACTGCC AGGAGGGGCT CTGCATCTGT GAGGAGGGCT 
     1981 ACCAGGGCCC TGACTGCTCA GCAGTTACCC CTCCAGAGGA CTTGCGAGTG GCTGGTATCA 
     2041 GCGACAGGTC CATTGAGCTG GAATGGGACG GGCCGATGGC AGTGACGGAA TATGTGATCT 
     2101 CTTACCAGCC GAGCCTGGGG GGCCTTCAGC TCCAGCAGCG GGTGCCTGGA GATTGGAGTG 
     2161 GTGTCACCAT CACGGAGCTG GAGCCAGGTC TCACCTACAA CATCAGCGTC TACGCTGTCA 
     2221 TTAGCAACAT CCTCAGCCTT CCCATCACTG CCAAGGTGGC CACTCATCTT TCTACTCCTC 
     2281 AAGGGCTACA GTTCAAGACG ATCACAGAGA CCACCGTGGA AGTGCAGTGG GAGCCCTTCT 
     2341 CTTTCTCCTT CGATGGGTGG GAGATCAGCT TCACTCCAAA GAACAATGAA GGAGGGGTGA 
     2401 TAGCTCAGCT CCCCAGCGAT GTTACGTCCT TTAACCAAAC GGGACTGAAA CCTGGGGAGG 
     2461 AGTACATTGT GAATGTTGTA GCACTAAAGG AACAAGCCCG GGGCCCTCCG ACCTCTGCCA 
     2521 GCGTCTCCAC TGTCATTGAC GGGCCCACAC AGATCCTGGT TCGAGATGTC TCTGATACTG 
     2581 TGGCCTTTGT GGAATGGACC CCACCTCGAG CCAAAGTTGA TTTCATTCTA TTAAAATATG 
     2641 GCTTGGTGGG TGGCGAAGGT GGGAAGACTA CCTTCCGGCT GCAGCCTCCC CTTAGCCAGT 
     2701 ACTCAGTGCA AGCCCTTAGA CCTGGCTCCC GCTACGAGGT GTCCATCAGC GCAGTCCGGG 
     2761 GGACCAATGA AAGTGATGCC TCAAGCACCC AGTTTACAAC AGAAATTGAT GCTCCCAAGA 
     2821 ATTTGCGAGT GGGTTCCCGG ACAGCAACTA GCCTTGACCT CGAATGGGAT AACAGCGAGG 
     2881 CTGAAGCTCA GGAGTACAAG GTTGTGTACA GCACCCTAGC CGGGGAGCAG TACCATGAAG 
     2941 TGCTGGTACC CAAAGGCATT GGTCCAACTA CCAAGACTAC CCTCACAGAT CTGGTTCCAG 
     3001 GCACAGAATA TGGAGTTGGA ATATCTGCGG TAATGAACTC GAAACAAAGC ATTCCCGCTA 
     3061 CCATGAATGC CAGGACTGAG CTTGACAGTC CCCGAGACCT CATGGTAACA GCTTCCTCAG 
     3121 AGACCTCTAT CTCTCTCATC TGGACGAAGG CCAGTGGTCC TATTGATCAC TATCGAATTA 
     3181 CTTTTACTCC ATCTTCTGGG ATCTCCTCAG AAGTCACTGT GCCTAGGGAT AGGACTTCAT 
     3241 ATACACTGAC AGATCTAGAG CCTGGAGCAG AATACATCAT CTCCATTACT GCTGAGAGGG 
     3301 GTCGGCAGCA GAGCCTGGAG TCTACTGTGG ATGCCTTCAC AGGCTTCCGC CCTATCTCCC 
     3361 ACTTGCACTT TTCTCATGTG ACCTCCTCCA GTGTCAACAT CACCTGGAGT GACCCATCTC 
     3421 CCCCAGCAGA CAGACTCATT CTGAACTACA GCCCCAGGGA TGAAGAGGAG GAGATGATGG 
     3481 AGGTTCTCTT GGATGCCACC AAGAGGCACG CCGTCCTAAT GGGTCTACAG CCAGCCACTG 
     3541 AATATATAGT GAATCTCGTA GCTGTCCATG GGACAGTAAC CTCTGAACCC ATAGTGGGTT 
     3601 CTATCACTAC AGGAATTGAT CCTCCCAAAA ACATCACAAT TAGCAACGTG ACTAAGGACT 
     3661 CCCTGACAGT TTCCTGGAGC CCTCCTGTTG CGCCTTTTGA TTACTACGAG TATCCTATCG 
     3721 ACCACCCAAG TGGACGGCTG GACAGCTCTG TCGTGCCCAA CACTGTGACA GAGTTCACAA 
     3781 TCACCAGGCT CTATCCAGCT AGTCAATATG AAATAAGCCT CAACAGTGTA CGCGGGAGGG 
     3841 AGGAGAGTGA ACGCATCTGC ACCCTGGTGC ACACAGCCAT GGATAGCCCC ATGGATCTAA 
     3901 TTGCTACCAA CATCACGCCA ACAGAAGCCC TGCTCCAGTG GAAGGCACCC ATGGGTGAAG 
     3961 TGGAAAATTA TGTCATTGTC CTCACCCACT TTGCCATGGC TGGAGAGACC ATCCTGGTTG 
     4021 ATGGGGTCAG TGAAGAATTC CAGCTTGTAG ACCTGCTTCC TAGGACCCAC TACACTGTCA 
     4081 CCATGTATGC TACCAGTGGA CCTCTCGTGA GTGGCACCAT CGCCACCAAC TTCTCCACCC 
     4141 TCCTGGACCC TCCCGCCAAC CTGACAGCCA GTGAAGTCAC CAGGCAAAGC GCACTGATCT 
     4201 CCTGGCAGCC ACCCAGAGCT GCGATTGAAA ACTATGTCTT GACATACAAG TCCACCGATG 
     4261 GAAGCCGCAA GGAGCTGATT GTGGATGCTG AGGACACCTG GATCCGACTG GAGGGCCTGT 
     4321 CGGAGAACAC AGACTACACA GTGCTCCTGC AGGCAGCTCA GGAGGCCACA AGGAGCAGCC 
     4381 TCACCTCTAC TATCTTTACC ACAGGGGGCC GGGTGTTCTC TCATCCTCAA GACTGTGCCC 
     4441 AGCACTTGAT GAATGGAGAC ACTCTGAGTG GAGTTTACAC CATCTTCCTC AATGGGGAGT 
     4501 TAAGCCACAA GTTGCAAGTA TACTGCGATA TGACCACAGA TGGGGGCGGC TGGATTGTTT 
     4561 TCCAGAGACG GCAAAATGGC CAAACTGATT TTTTCCGGAA ATGGGCAGAT TACCGTGTTG 
     4621 GCTTTGGGAA TCTGGAGGAT GAGTTTTGGC TAGGGCTAGA CAACTACCAC AGGATAACAG 
     4681 CCCAGGGCCG CTATGAGCTG CGTGTGGATA TGCGGGATGG ACAGGAGGCC GTCTTTGCCT 
     4741 ACTATGACAA GTTCGCTGTG GAGGACAGCA GAAGCCTGTA CAAGCTCCGT ATAGGAGGCT 
     4801 ACAATGGCAC TGCAGGAGAC TCCCTTAGCT ATCACCAGGG ACGTCCTTTC TCCACTGAGG 
     4861 ACAGAGACAA TGATGTTGCA GTCACCAACT GTGCCATGTC ATACAAGGGT GCTTGGTGGT 
     4921 ATAAGAACTG CCACCGGACC AACCTCAACG GGAAGTATGG GGAGTCCAGG CACAGTCAGG 
     4981 GGATCAACTG GTACCATTGG AAAGGCCATG AATTCTCCAT CCCCTTTGTA GAAATGAAGA 
     5041 TGAGGCCCTA CATCCATCGT CTCACAGCCG GGAGGAAACG GCGAGCCTTG AAATTCACGC 
     5101 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     5161 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     5221 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     5281 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     5341 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5401 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5461 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5521 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5581 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5641 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5701 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5761 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5821 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5881 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5941 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     6001 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     6061 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     6121 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     6181 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     6241 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     6301 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6361 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6421 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6481 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6541 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6601 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6661 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6721 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6781 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6841 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6901 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6961 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     7021 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     7081 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     7141 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     7201 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     7261 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     7321 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7381 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7441 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7501 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7561 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7621 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7681 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7741 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7801 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7861 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7921 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7981 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     8041 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     8101 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     8161 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     8221 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     8281 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8341 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8401 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8461 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8521 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8581 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8641 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8701 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8761 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8821 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8881 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8941 GCGAATTTT
//