LOCUS       RR203715                7248 bp    DNA     circular  SYN 
DEFINITION  Atxn1 (Myc-DDK-tagged ORF) - Rat ataxin 1 (Atxn1), (10 ug)
ACCESSION   NM_012726
VERSION     NM_012726.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3405..3496 
                     /label="Myc-DDK"  
     misc_feature    3526..4111 
                     /label="PolyA signal"  
     misc_feature    3583..3602 
                     /label="Restriction Enzyme"  
     misc_feature    4387..5059 
                     /label="ColE1"  
     misc_feature    5489..6283 
                     /label="Kan/Neo"  
     misc_feature    6318..6620 
                     /label="SV40 ori"  
     misc_feature    6706..7183 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3387..3393 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3387 
                     /label="Atxn1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAATCTAAC CAAGAGCGGA GCAATGAATG CCTGCCTCCC AAGAAACGTG 
     1081 AGATCCCCGC CACCAGTCGG CCCTCCGAGG AGAAGGCCAC TGCTCTGCCC AGCGACAACC 
     1141 ACTGCGTGGA GGGTGTGGCA TGGCTCCCCA GCACCCCTGG CAGCCGCGGC CACGGGGGTG 
     1201 GGCGGCACGG GCCAGCAGGG ACTTCCGGGG AACATGGTTT ACAAGGAATG GGTTTACATA 
     1261 AAGCACTGTC CGCAGGGCTG GATTACTCCC CACCCAGTGC CCCCAGGTCG GTCCCCACAG 
     1321 CCAACACGCT GCCCACCGTG TACCCTCCTC CTCAGTCAGG GACGCCGGTG TCTCCTGTGC 
     1381 AGTACGCCCA CCTATCACAT ACCTTCCAGT TCATTGGGTC CTCCCAGTAT AGTGGGCCTT 
     1441 ACGCGGGCTT TATCCCTTCC CAGCTGATCT CCCCACCAGG CAACCCAGTC ACCAGTGCGG 
     1501 TGGCCTCGGC TGCAGGGGCC ACCACTCCAT CACAGCGCTC CCAGCTGGAG GCATATTCCA 
     1561 CCCTGCTGGC CAACATGGGC AGTCTGAGCC AGGCACCAGG ACACAAGGTT GAGCCCCCTC 
     1621 CGCAGCAGCA CCTCGGCAGG GCTGCGGGAT TAGTCAACCC GGGGTCCCCT CCACCTACCC 
     1681 AGCAGAACCA GTACATTCAC ATTTCCAGCT CTCCGCAGAG CTCCGGGCGG GCAACATCTC 
     1741 CACCCATCCC GGTCCACCTC CATCCCCATC AGACGATGAT CCCGCACACG CTCACCCTGG 
     1801 GGCCTTCATC CCAGGTGGTC GTGCAATACA GTGACGCCGG AGGCCACTTT GTTCCTCGAG 
     1861 AGTCCACCAA AAAAGCAGAA AGCAGCAGGT TGCAGCAGGC TATGCAGGCC AAGGAGGTCC 
     1921 TCAATGGGGA GATGGAGAAA AGCCGGAGGT ATGGGGCGTC ATCTTCTGTG GAGCTGAGCC 
     1981 TGGGGAAGAC GAGCAGCAAG TCAGTGCCTC ACCCCTATGA GTCCAGGCAT GTGGTGGTCC 
     2041 ACCCGAGCCC AGCAGACTAC AGCAGTCGTG ATACCTCCGG GGTCCGTGGA TCTGTGATGG 
     2101 TCCTGCCCAA CAGCAGCACA CCCTCAGCCG ACCTGGAGAC ACAGCAGGCC ACACATCGAG 
     2161 AGGCCTCCCC ATCCACCCTC AATGACAAGA GCGGTTTGCA CCTAGGGAAG CCCGGCCACA 
     2221 GGTCCTACGC GCTGTCCCCG CACACGGTCA TTCAGACCAC ACACAGCGCA TCAGAGCCTC 
     2281 TCCCGGTGGG CCTACCAGCC ACGGCCTTCT ATGCTGGCGC TCAACCTCCT GTCATCGGCT 
     2341 ATCTGAGTAG CCAGCAGCAA GCAATCACCT ATGCTGGTGG TCTGCCCCAG CACCTGGTGA 
     2401 TCCCAGGTAC CCAGCCCCTG CTCATCCCAG TGGGCAGCCC TGACATGGAC ACACCTGGGG 
     2461 CAGCCTCGGC CATAGTGACG TCATCGCCCC AGTTTGCTGC AGTACCTCAC ACGTTTGTCA 
     2521 CCACCGCCCT GCCCAAGAGC GAGAACTTCA ACCCAGAGGC TCTGGTCACC CAGGCAGCCT 
     2581 ACCCAGCCAT GGTGCAGGCC CAGATCCACC TGCCGGTGGT ACAGTCCGTG GCATCCCCTG 
     2641 CCGCGGCATC ACCCACGCTG CCGCCATATT TCATGAAAGG CTCCATCATC CAGCTGGCCA 
     2701 ACGGGGAGCT GAAGAAGGTA GAGGATCTGA AGACAGAGGA TTTCATCCAG AGTGCAGAGA 
     2761 TTAGCAATGA CCTCAAGATC GACTCCAGTA CTGTGGAGAG GATCGAGGAC AGCCACAGCC 
     2821 CCGGTGTGGC GGTGATACAA TTTGCTGTTG GTGAACACCG AGCCCAGGTC AGTGTCGAAG 
     2881 TTTTGGTAGA GTATCCTTTT TTTGTATTTG GACAGGGCTG GTCATCCTGC TGTCCCGAGC 
     2941 GGACCAGCCA GCTCTTTGAT CTGCCGTGTT CCAAACTCTC CGTTGGGGAC GTCTGCATCT 
     3001 CGCTCACCCT CAAGAACCTG AAGAATGGCT CTGTTAAAAA GGGCCAGCCC GTGGACCCTG 
     3061 CCAGTGCCCT GCTGAAGCAC GCAAAGACCG ACAGCCTGGC TGGCAGCAGA CACAGATACG 
     3121 CCGAGCAGGA AAACGGAATC AACCAGGGGA GCGCCCAGGT GCTCTCTGAG AACGGCGAAC 
     3181 TGAAGTTTCC AGAAAAAATT GGATTGCCTG CAGCACCCTT CCTCACCAAA ATAGAACCGA 
     3241 GCAAGCCCAC AGCCACGAGG AAGAGGAGGT GGTCGGCGCC GGAGACCCGT AAACTGGAGA 
     3301 AGTCGGAGGA CGAGCCACCT TTGACTCTTC CCAAGCCTTC GCTCATTCCT CAGGAGGTTA 
     3361 AGATCTGCAT CGAAGGCCGA TCTAACGTGG GCAAGACGCG TACGCGGCCG CTCGAGCAGA 
     3421 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     3481 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     3541 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     3601 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     3661 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     3721 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     3781 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     3841 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     3901 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     3961 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     4021 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4081 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4141 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     4201 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     4261 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     4321 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     4381 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     4441 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     4501 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     4561 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     4621 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     4681 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     4741 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     4801 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     4861 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     4921 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     4981 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     5041 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     5101 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     5161 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     5221 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     5281 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     5341 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     5401 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     5461 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     5521 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     5581 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     5641 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     5701 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     5761 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     5821 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     5881 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     5941 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     6001 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     6061 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     6121 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     6181 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     6241 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     6301 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     6361 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     6421 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     6481 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6541 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     6601 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     6661 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     6721 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     6781 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     6841 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     6901 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     6961 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     7021 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     7081 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     7141 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     7201 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//