LOCUS       RR203710                8529 bp    DNA     circular  SYN 
DEFINITION  Mamdc4 (Myc-DDK-tagged ORF) - Rat MAM domain containing 4 (Mamdc4), (10 ug)
ACCESSION   NM_145768
VERSION     NM_145768.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4686..4777 
                     /label="Myc-DDK"  
     misc_feature    4807..5392 
                     /label="PolyA signal"  
     misc_feature    4864..4883 
                     /label="Restriction Enzyme"  
     misc_feature    5668..6340 
                     /label="ColE1"  
     misc_feature    6770..7564 
                     /label="Kan/Neo"  
     misc_feature    7599..7901 
                     /label="SV40 ori"  
     misc_feature    7987..8464 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4668..4674 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4668 
                     /label="Mamdc4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTGCCTGCCT AGCTGCCTCC TCTCAATCTG GGTCCTATTT ATGGCTGCAC 
     1081 AGTCTCTAGG CAAGACCTGG GTCCCTGACC ACTGCAGAAG CCCCACCGAA GCTACGTGCA 
     1141 ACTTTGTGTG TGACTGTGGA GACTGTTCAG ATGAGGCTCA GTGTGGTTTC CACGGTGCCT 
     1201 CAACTACCCC AAACACCCCC TTCACCTGCA ACTTTGAGCA GGATCCCTGT GGCTGGCAGG 
     1261 ACATCAGCAC CTCGGGCTAT CGATGGCTTC GAGACCGGGC AGGGGCAGGG CTGGATAGTT 
     1321 CTGGGCCCCA TTCTGACCAT ACACGTGGCA CTGACTTGGG CTGGTACATG GCTGTGGGAA 
     1381 CCCATAGTGG GAAGGAGCCA TCTACTCGGA CTTTGCGCTC TCCTGTCATG CGTGAGGCAG 
     1441 CCCCCACCTG TGAACTGAGG CTCTGGTACC ACACTGACTC TAGAGATGTC GCTGAGCTGC 
     1501 GGCTGGATCT GACCCATGGT ATGGAGACAC TGACTCTATG GCAGAGCTCG GGACCTTGGG 
     1561 GTCCCTGGCC TGGCAGGGAA CTAGCAGTGA ACACTGGCCG AATCCAGGGT GACTTCAAAG 
     1621 TGACATTTTC TGCCACTCGA AATGCTACAC ACAGGGGCGC TGTGGCCTTG GATGACATGG 
     1681 AGTTCTGGGA CTGTGGTCTG CCCATCCCTC AGGCCAGGTG TCCCCTGGGG CACCACCACT 
     1741 GCCAGAACAA AGCTTGTGTG GAGCCCCACC AGCTCTGTGA CGGTGAAGAC AACTGTGGAG 
     1801 ACAGTTCTGA TGAAGACCCG CTCATCTGCA GCCACCACAT GGCCACCGAC TTTGAGACAG 
     1861 GCCTAGGACC ATGGACCCAA TTAGAGGGCT GGACACGGAA CTTCAGTGCT GGCAGCATGG 
     1921 TGAGCCCTGC CTGGCCCCAC CGAGATCACA GCCGGAATAG TGCATATGGC TTCTTTCTCG 
     1981 TCTCTGTGGC CAAGCCTGGC ACCACTGCTG TCCTCTACAG CCCTGAGTTC CAAGGCTCAG 
     2041 TCTCCTACAA CTGCTCGTTC ACCTTCTATT ACTACCTGCA TGGGTCTGAG GCCAACCAAT 
     2101 TCCAGCTCTT TGTGCAGGCA CAGGGGCTCA ACACCACCCA GCCTCCTGTC CTACTCCGAA 
     2161 GCCGCCATGG AGAGTTGGGG ACAGCCTGGG TCAGAGACCG GGTTAACATT CAAAGTGCCC 
     2221 ATCCATTTCG GATCCTTCTT GCTGGGGAGA CTGGTCCAGG AGGCTTCGTG GGCCTAGACG 
     2281 ACCTTATCAT GTCCAACCAC TGCATACTTG TTCCAGGTAT GTCCACCCTG CAGTCATCAC 
     2341 TTTCTGGACC TGTGCCATTG GCCCTCTATC CCCAGACATC CATCAAGCGA ACCTGTGACG 
     2401 CAGGACATCT CTCCTGTGAC GAGCTGTGTG TGCCCCCGGA GCAGCTGTGT GATTTCCAGC 
     2461 AGCACTGTGC CGAAGGCGAG GATGAAGAGA AGTGTGGCAC CACGGACTTT GAGTCTGCCT 
     2521 CTGCTGGGGG CTGGGAGGAC ATCAGTATAG GAAAGCTACA GTGGCAACGG GCTGAAGCCC 
     2581 AGGAGAGCGG GAAGCCTGCC AGGGATACCA ACCGGAATGC ACCTGGGCAC TTCCTATCTC 
     2641 TGCGGAAGGC CTGGGGGCAG CTACGATCTG AGGCCCGGGC TCTCACACCT ACTCTGGGCC 
     2701 CCTCTGGCCC TCACTGTGAA CTCCACATGA CTTACTATTT TCACAGTCAT CCCCAAGGCT 
     2761 TCTTGGCACT GGCTGTGGTG GAGAATGGCT TCCGGGAGCT GCTGTGGCAG GCTCCAAGCA 
     2821 GCAGCAGTGG GGGCTGGACA CTACAGAAGA TTCTTCTTGG GGCACGGCGC TGGCCATTCC 
     2881 AGCTGGAGTT CGTCAGTCTG GTGGACTTGG ATGGCCCTGG CCAACAGGGG GCTGGGGTGG 
     2941 ATAATGTGAC CCTGAGAGAC TGCAACCCCA TGGTGACCAC TGAGAGTGAC CAAGAGGTCT 
     3001 CCTGTAACTT TGAACGGGAC TCCTGCAGCT GGCACACAGG CCACCTCACA GATGCCCACT 
     3061 GGCACCGTGT AAAAAGCCAC GGCTCTCAGT ATGACCACAC CACCGGCCAA GGTTTTTTCA 
     3121 TGTTCCTGGA TCCCATGGAC CCACCTGCTC GAGGCCAAGG TGCCCTGTTG CTCACAAGAC 
     3181 CCCAGGTGCC AGTTGTCCCC AAGGAATGTC TCAGCTTCTG GTACCATCTG CATGGGCCCC 
     3241 AGATTGGGAC ACTGTGCTTG GCCATGAGGA GGGAGGGGGA GGAAGACACA CTACTGTGGT 
     3301 CCCGGTCAGG CACCCACGGC AACCGCTGGC ACCAGGCTTG GGTCACTCTT CACCACCAGC 
     3361 TGCAGCCCAG CACCAAGTAC CAGTTGCTGT TTGAAGGCCT CCGGGATGGG TACCATGGCA 
     3421 CAATGGGCCT GGATGACATG GCTGTGCGGC CTGGCCCCTG CTGGGCCGCC AAGAGATGTT 
     3481 CCTTTGAAGA CTCTGACTGT GGCTTCTCCC CAGGGGACTG GGGGCTGTGG ACACGCCAGA 
     3541 ATAATGCCTC AGGCCTTGGT CCTTGGGGTC CTTGGATAGA CCATACCACA GGGACAGCCC 
     3601 AGGGGCACTA CATGGTGGTG GACACCAGCC CAAATTTACT GCCCAAAGGT CATGTGGCCT 
     3661 CTCTGACTTC AGAGGAGCAC CCGCCCCTGT CCCGGCCTGC CTGCCTGTCC TTCTGGTACC 
     3721 ACCTGAGCTT CCACAATCCA GGCACCTTGA GGGTCTTCGT GGAAGAGAGC ACAAGGCGCC 
     3781 AAGAGCTCAG CATCAGTGGC CATGGTGGAT TTGCGTGGCG CCTAGGCAGC GTGAATGTAC 
     3841 AGGCTGAGCA GGCCTGGAAG GTAGTGTTTG AGGCTATGGC TTCTGGCGTG GAACATTCCT 
     3901 ACATGGCTCT GGATGACATT TCCCTCCAAG ATGGGCCCTG TGCTCAGCCA GGGTCCTGTG 
     3961 ACTTTGAATC TGGCCTGTGT GGGTGGAGCC ACCTGCCTTG GCCTGGTCTG GGTGGGTACA 
     4021 GCTGGGACTG GAGCAGTGGA GCCACTCCAT CCCGGTACCC CCGGCCTTCA GTGGACCACA 
     4081 CTGTGGGCAC AGAAGCAGGC CACTTCGCTT TCTTTGAAAC CAGCGTGCTA GGTCCTGGGG 
     4141 GCCAGGCAGC CTGGCTCGGC AGTGAGCCAC TGCCAGCAAC TGCAGTCTCC TGCCTCCACT 
     4201 TCTGGTACTA CATGGGCTTT CCTGCGCATT TCTACAAGGG TGAGTTGAGA GTGCTCCTGA 
     4261 GCAGTACCCA AGGACAGCTA GCTGTGTGGC ACCGGGGTGG GCACTTGCGG GACCAGTGGC 
     4321 TACAAGTCCA GATTGAAGTG TCCAGCTCTG AGGAGTTCCA GATTGTTTTT GAAGCCACTC 
     4381 TGGGTGGCCA GCCGGCTCTA GGGCCCATTG CCCTTGATGA TGTGGAGTAC TTGGCTGGAC 
     4441 AACACTGCAA GCAACCTACA CCTAGCCAGG GTAGAGTGGC AGCACCCGTG TCTGTGCCGG 
     4501 TTGCAGTCGG AGGAGCCCTC CTCCTCTTCC TGTTGCTCCT GGGCCTTGGA GGTTGGCACT 
     4561 GGCTGCAGAA GCAGCACCTC CCCTGCCAAA GTACAGATGC AGCAGCCTCT GGCTTTGACA 
     4621 ATATCCTCTT CAATGCGGAT CAAGTTACCC TCCCAGAATC AATCACCAGT AACCCAACGC 
     4681 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4741 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4801 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4861 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4921 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4981 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5041 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5101 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5161 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5221 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5281 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5341 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5401 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5461 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5521 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5581 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5641 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5701 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5761 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5821 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5881 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5941 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6001 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6061 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6121 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6181 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6241 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6301 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6361 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6421 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6481 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6541 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6601 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6661 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6721 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6781 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6841 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6901 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6961 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7021 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7081 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7141 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7201 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7261 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7321 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7381 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7441 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7501 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7561 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7621 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7681 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7741 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7801 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7861 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7921 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7981 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8041 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8101 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8161 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8221 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8281 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8341 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8401 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8461 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8521 GCGAATTTT
//