LOCUS       RR203624                7791 bp    DNA     circular  SYN 
DEFINITION  Dspp (Myc-DDK-tagged ORF) - Rat dentin sialophosphoprotein (Dspp), (10 ug)
ACCESSION   NM_012790
VERSION     NM_012790.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3948..4039 
                     /label="Myc-DDK"  
     misc_feature    4069..4654 
                     /label="PolyA signal"  
     misc_feature    4126..4145 
                     /label="Restriction Enzyme"  
     misc_feature    4930..5602 
                     /label="ColE1"  
     misc_feature    6032..6826 
                     /label="Kan/Neo"  
     misc_feature    6861..7163 
                     /label="SV40 ori"  
     misc_feature    7249..7726 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3930..3936 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3930 
                     /label="Dspp"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAAACGAAG ATAATTATAT ATATTTGCAT TTGGGCAACC GCATGGGCCA 
     1081 TTCCGGTCCC TCAGTTAGTG CCGCTGGAGA GAGACATTGT TGAAAAATCT GCCGACGTAC 
     1141 CCTTTCTAGC ACATCCAGGA ACCGCAGCAC AGAATGAGTT GCATATCAAC AACGCCACTA 
     1201 ACGACGATTC CCCAAAGGGC AGTGAGCTAG GAAGGCAGGT ACACTCTAAT GGGGGTTACG 
     1261 AAAGAGACAG GAATGGCTCC GAGTCGATAG CCGTAGGAGG GAAGAGTTCT CCTACTCAGC 
     1321 CCATTTTAGC AAACGCACAG GGGAACAGTG CTAAAGAACG CGAGGACGTA GAAACATACG 
     1381 GTCACGATGG GATACACGCA GGAGGAGAGA ACAGCACAGC AAACGGCATC AGGGGCCAAG 
     1441 TAGGCATCGC TGAAAATGCC GAGGAAGCAA AGGAGAGTAA GGTACACGGG CAGCCTCATC 
     1501 AGGATACAAA AACTGGCCTT GCCAGTGACA CGAGCCAGAA TGGAGACGCC ACCCTTGTCC 
     1561 AGGAAAATGA GCCTCAGGTA GCCGGAAGCA AGAATAGCAC AAACCATGAG GTTGGAACAC 
     1621 ACGGGAGTGG AGTTGCTGCA CAGGAAACGA CACCGCAGAG AGAAGGGGAG GGGAGTGAGA 
     1681 ACCAGGGGGC TGAGGTGACA CCAAGCATTG GGGAAGGTGC TGGTTTGGAT AATACTGAAG 
     1741 GGAGTCCTAG CGGGAACGGG ATAGAGGAGG ATGAAGACAC GGGTTCTGGT GATGGTGTGG 
     1801 GTGCAGACGC AGGAGATGGA AGGGAGAGTC ACGATGGCAC GGAGGGCCAC GAGGGCCAGT 
     1861 CTAGTGGGGG AAACAATGAC AACAGAGGTC AGGGTTCAGT TAGTACTGAA GACGATGACT 
     1921 CTAAAGAACA AGAAGGCTCC CCAAATGGAC GTGGTGGGGA CAACACCTCC AGCAGTGAAG 
     1981 AAACCGGTAT TGAAGAAGGC GATGGTACCC AAACAACACA GGACAACCAG AATCTCAGTC 
     2041 CCACAGAGGG TGGGATTATC AGCCAGGCAG AAGCATGTCC TTCTGGGCAG AGCCAAAATC 
     2101 AGGGATTAGA AACCGAAGGT TCCAGCACGG GCAACAAAAG TAGTATTACC AAAGAATCTG 
     2161 GGAAGCTCAG TGGAAGTAAA GACAGCAACG GACACCATGG AATGGAGCTG GACAAAAGGA 
     2221 ATAGCCCAAA GCAAGGGGAG TCTGACAAGC CCCAAGGGGC TGCCGAGAAG TCAGACACCC 
     2281 ATAACAACAT GGGACACAGC AGGATAGGCA GCAGCAGCAA TAGTGACGGG CATGACAGTT 
     2341 ATGATTTTGA CGATGAGTCC ATGCAAGGAG ACGATCCCAA CAGCAGTGAC GAGTCTAACG 
     2401 GAAGTGACGG AAGTGATGAT GCTAACTCCG AAAGCGCCAT TGAGAATGGC AACCATGGAG 
     2461 ATGCTTCTTA CACCTCTGAT GAATCAAGCG ATAATGGCAG CGACAGTGAC TCACATGCTG 
     2521 GAGAGGATGA CAGCAGTGAT GACACATCTG ATACCGATGA CAGTGACAGT AACGGTGATG 
     2581 ATGACAGTGA GAGTAAGGAC AAGGACGAAT CAGACAACAG TAACCACGAC AATGACAGTG 
     2641 ACAGTGAGAG CAAATCAGAC AGCAGCGACA GTGACAGTGA CAGCAGTGAC AGCAGTGACA 
     2701 GCAGTGACAG TAGCGACAGC AGCGAAAGCA GTGACAGTAG CGACAGTAGC GACAGCAGTG 
     2761 ACAGCAGTGA CAGCAGTGAC AGTAGCGACA GCAGTGACAG CAGTGACAGC AGCGACAGTG 
     2821 ACAGCAGCGA CAGTGACAGC AGTGACAGTA GTGACAGCAG TGACAGTAGT GACAGCAGTG 
     2881 ACAGTAGTGA CAGCAGTGAC AGCAGCGACA GTAGTGACAG TAGTGACAGC AGCAACAGTG 
     2941 ACAGCAGTGA CAGTAGCGAC AGCAGTGACA GTAGCGACAG CAGTGACAGT AGTGACAGCA 
     3001 GTGACAGCAG CGACAGCAGT GACAGCAGTG ACAGCAGTGA CAGCAGCGAA AGCAGTGACA 
     3061 GCAGTGACAG TAGCGACAGC AGCGACAGTG ACAGCAGTGA CAGCAGTGAC AGTAGTGACA 
     3121 GCAGTGACAG CAGTGATAGC AGCGACAGTA GTGATAGCAG TGACAGCAGT GACAGCAGTG 
     3181 ACAGCAGCGA CAGTGACAGC AGCGACAGTG ACAGCAGTGA CAGTAGTGAC AGCAGTGACA 
     3241 GTAGTGACAG CAGTGACAGT AGTGACAGCA GTGACAGCAG CGACAGTAGT GACAGTAGTG 
     3301 ACAGCAGCAA CAGTGACAGC AGTGACAGTA GCGACAGCAG TGACAGTAGC GACAGCAGTG 
     3361 ACAGTAGTGA CAGCAGTGAC AGCAGCGACA GCAGTGACAG CAGTGACAGC AGTGACAGCA 
     3421 GCGACAGTAG TGATAGCAGT GACAGCAGTG ACAGCAGTGA CAGCAGTGAC AGTAGTGACA 
     3481 GCAGTGACAG CAGTGATAGC AGTGACAGCA GTGACAGTAG CGACAGCAGC GATAGCAACA 
     3541 GTAGTGACAG CAGTGACAGC AGTGACAGTA GTGACAGCAG CGACAGCAGT GACAGTAGTG 
     3601 ACAGTAGTGA CAGCAGTGAC AGCAGTGACA GCAGTGACAG CAGCAACAGC AGCGACAGCA 
     3661 GTGACAGCAG TGACAGCAGC GATGGTGACA GCAGTGACGG TGACAGCAGC GATAGTGACA 
     3721 GCAGTGATAG TGACAGTAGT AACAGCAGTG ATAGTGACAG CAGTGACAGT AGTGACAGCA 
     3781 GCAGCAGTGA CAGCAGCGAT AGTGATAGTG ACAGCAAGGA CAGCACTTCT GACAGCAGTG 
     3841 ATGACAACAG CAAGTCTGGT AATGGCAACA GTGACAGCGA CAGTGACAGC GACAGTGACA 
     3901 GCGAAGGCAG TGACAGTAAC CACTCAACCA GTGATGATAC GCGTACGCGG CCGCTCGAGC 
     3961 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4021 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4081 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4141 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4201 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4261 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4321 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4381 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4441 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4501 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     4561 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     4621 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     4681 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     4741 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     4801 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     4861 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     4921 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     4981 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5041 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5101 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5161 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5221 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5281 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5341 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     5401 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     5461 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     5521 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     5581 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     5641 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     5701 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     5761 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     5821 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     5881 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     5941 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6001 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6061 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6121 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6181 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6241 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6301 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6361 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6421 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     6481 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     6541 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     6601 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     6661 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     6721 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     6781 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     6841 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     6901 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     6961 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7021 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7081 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7141 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7201 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7261 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7321 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7381 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7441 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     7501 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     7561 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     7621 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     7681 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     7741 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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