LOCUS       RR203611                7884 bp    DNA     circular  SYN 
DEFINITION  Taok1 (Myc-DDK-tagged ORF) - Rat TAO kinase 1 (Taok1), (10 ug)
ACCESSION   NM_173327
VERSION     NM_173327.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4041..4132 
                     /label="Myc-DDK"  
     misc_feature    4162..4747 
                     /label="PolyA signal"  
     misc_feature    4219..4238 
                     /label="Restriction Enzyme"  
     misc_feature    5023..5695 
                     /label="ColE1"  
     misc_feature    6125..6919 
                     /label="Kan/Neo"  
     misc_feature    6954..7256 
                     /label="SV40 ori"  
     misc_feature    7342..7819 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4023..4029 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4023 
                     /label="Taok1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCATCAACT AACAGAGCAG GCAGTCTAAA GGACCCTGAA ATCGCAGAGC 
     1081 TCTTCTTCAA AGAAGATCCG GAAAAACTCT TCACAGATCT CAGAGAAATC GGCCATGGGA 
     1141 GCTTTGGAGC AGTTTATTTT GCACGAGATG TGCGTACTAA TGAAGTGGTG GCCATCAAGA 
     1201 AAATGTCTTA TAGTGGAAAG CAGTCTACTG AGAAATGGCA GGATATTATT AAGGAAGTCA 
     1261 AGTTTCTACA AAGAATAAAA CATCCCAACA GTATAGAATA CAAAGGCTGC TATTTACGTG 
     1321 AACACACAGC ATGGCTTGTA ATGGAATATT GTTTAGGATC TGCTTCGGAT TTACTAGAAG 
     1381 TTCATAAAAA GCCATTACAA GAAGTGGAAA TAGCAGCAAT TACACATGGT GCTCTCCAGG 
     1441 GATTAGCTTA TTTACATTCT CATACCATGA TCCATAGAGA TATCAAAGCA GGAAATATCC 
     1501 TTCTGACAGA ACCAGGCCAA GTGAAACTTG CTGACTTTGG ATCTGCTTCC ATGGCCTCCC 
     1561 CTGCCAATTC TTTTGTGGGA ACACCATATT GGATGGCCCC AGAAGTAATT TTAGCCATGG 
     1621 ATGAAGGACA ATATGATGGC AAAGTTGATG TATGGTCTCT TGGAATAACA TGTATTGAAT 
     1681 TAGCCGAGAG GAAGCCTCCT TTATTTAATA TGAATGCAAT GAGTGCCTTA TATCACATAG 
     1741 CCCAAAATGA ATCCCCTACA CTACAGTCTA ATGAATGGTC TGATTATTTT CGAAACTTTG 
     1801 TAGATTCTTG CCTCCAGAAA ATCCCTCAAG ATCGCCCTAC ATCAGAGGAA CTTTTAAAGC 
     1861 ACATGTTTGT TCTTCGAGAG CGCCCTGAAA CAGTGTTAAT AGATCTTATT CAAAGGACAA 
     1921 AGGATGCAGT AAGAGAGCTG GACAATCTAC AATATCGAAA GATGAAGAAA CTCCTTTTCC 
     1981 AGGAGGCACA TAATGGACCA GCAGTAGAAG CACAGGAAGA AGAGGAGGAG CAAGATCATG 
     2041 GTGGTGGCCG GACAGGAACA GTAAATAGTG TTGGAAGCAA TCAGTCTATC CCCAGTATGT 
     2101 CTATCAGTGC CAGTAGCCAA AGCAGCAGTG TTAATAGTCT TCCAGATGCA TCGGATGACA 
     2161 AGAGTGAGCT AGACATGATG GAGGGAGACC ATACAGTGAT GTCTAACAGT TCTGTCATCC 
     2221 ACTTAAAACC TGAGGAGGAA AATTACCAAG AAGAAGGAGA TCCTAGAACA AGAGCATCAG 
     2281 CTCCACAGTC TCCACCTCAA GTGTCTCGTC ACAAATCACA TTATCGTAAT AGAGAACACT 
     2341 TTGCAACTAT ACGAACAGCA TCACTGGTTA CAAGACAGAT GCAAGAACAT GAGCAGGACT 
     2401 CTGAACTTAG AGAACAGATG TCTGGTTATA AGCGGATGAG GCGACAGCAT CAGAAGCAGC 
     2461 TGATGACTCT GGAAAATAAA CTGAAGGCAG AAATGGACGA ACATCGGCTC AGATTAGACA 
     2521 AAGATCTTGA AACTCAGCGC AACAATTTCG CTGCAGAAAT GGAGAAACTT ATTAAGAAAC 
     2581 ACCAAGCTTC TATGGAAAAA GAGGCTAAAG TGATGGCCAA CGAGGAGAAA AAATTCCAAC 
     2641 AACACATTCA GGCTCAACAG AAGAAAGAAC TGAATAGCTT TTTGGAGTCT CAAAAAAGAG 
     2701 AATATAAACT TCGAAAAGAG CAGCTTAAGG AGGAGCTGAA TGAAAACCAG AGCACACCTA 
     2761 AAAAAGAAAA GCAGGAATGG CTTTCAAAGC AGAAGGAGAA TATTCAACAT TTTCAGGCAG 
     2821 AAGAAGAAGC TAATCTTCTT CGACGTCAAA GGCAGTATCT AGAGCTAGAA TGTCGTCGCT 
     2881 TCAAAAGAAG AATGTTACTT GGTCGGCATA ACTTGGAACA GGACCTTGTC AGGGAGGAGT 
     2941 TAAACAAAAG GCAGACTCAG AAGGACTTAG AACATGCAAT GTTACTGCGA CAGCATGAAT 
     3001 CCATGCAAGA ACTGGAGTTT CGCCACCTCA ACACTATTCA GAAGATGCGC TGTGAGTTGA 
     3061 TCAGACTGCA ACATCAAACT GAGCTTACTA ACCAGCTGGA ATACAATAAG AGAAGGGAAC 
     3121 GGGAACTAAG ACGGAAACAT GTCATGGAAG TTCGACAGCA GCCTAAGAGT TTGAAGTCTA 
     3181 AAGAACTCCA AATAAAAAAG CAGTTTCAGG ATACCTGCAA AATTCAAACC AGACAGTACA 
     3241 AAGCATTAAG GAATCACCTA CTGGAGACTA CACCAAAGAG TGAGCACAAA GCTGTTCTGA 
     3301 AAAGACTCAA GGAGGAACAG ACTCGGAAGT TAGCCATCTT GGCTGAGCAG TATGATCATA 
     3361 GCATTAATGA AATGCTCTCC ACACAAGCTC TGCGTTTGGA TGAAGCACAG GAAGCAGAAT 
     3421 GCCAGGTTTT GAAGATGCAG CTACAGCAGG AACTGGAGCT GTTGAATGCA TATCAGAGCA 
     3481 AAATCAAGAT GCAGGCTGAG GCCCAACATG ATCGAGAGCT TCGAGAGCTG GAACAAAGGG 
     3541 TCTCCCTTCG GAGAGCACTC TTAGAACAGA AGATTGAAGA AGAGATGTTG GCTTTGCAGA 
     3601 ATGAACGCAC AGAACGAATA CGTAGCCTGC TCGAGCGCCA GGCCAGAGAA ATTGAAGCTT 
     3661 TTGACTCTGA AAGCATGAGA TTAGGTTTTA GTAACATGGT CCTTTCTAAT CTCTCCCCTG 
     3721 AGGCATTCAG CCACAGCTAC CCAGGAGCTT CTAGCTGGTC TCACAATCCT ACTGGGGGTT 
     3781 CAGGACCTCA CTGGGGTCAT CCCATGGGTG GCACACCACA AGCTTGGGGT CATCCGATGC 
     3841 AAGGCGGACC CCAACCATGG GGTCACCCCT CAGGGCCAAT GCAAGGGGTA CCTCGAGGTA 
     3901 GCAGTATAGG AGTCCGCAAT AGCCCCCAGG CTCTGAGGCG GACAGCTTCT GGGGGACGGA 
     3961 CGGAACAGGG CATGAGCAGA AGCACGAGTG TCACTTCACA AATATCCAAT GGGTCACACA 
     4021 TGTCTTACAC AACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4081 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4141 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4201 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4261 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4321 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4381 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4441 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4501 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4561 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4621 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4681 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4741 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4801 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     4861 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     4921 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     4981 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5041 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5101 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5161 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5221 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5281 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5341 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5401 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5461 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5521 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5581 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     5641 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     5701 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     5761 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     5821 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     5881 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     5941 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6001 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6061 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6121 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6181 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6241 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6301 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6361 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6421 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6481 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6541 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6601 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6661 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     6721 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     6781 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     6841 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     6901 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     6961 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7021 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7081 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7141 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7201 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7261 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7321 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7381 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7441 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7501 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7561 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7621 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7681 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     7741 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     7801 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     7861 TAACAAAAAT TTAACGCGAA TTTT
//