LOCUS       RR203604                8541 bp    DNA     circular  SYN 
DEFINITION  Sf3b3 (Myc-DDK-tagged ORF) - Rat splicing factor 3b, subunit 3 (Sf3b3), (10 ug)
ACCESSION   NM_001106187
VERSION     NM_001106187.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4696..4787 
                     /label="Myc-DDK"  
     misc_feature    4817..5402 
                     /label="PolyA signal"  
     misc_feature    4874..4893 
                     /label="Restriction Enzyme"  
     misc_feature    5678..6350 
                     /label="ColE1"  
     misc_feature    6780..7574 
                     /label="Kan/Neo"  
     misc_feature    7609..7911 
                     /label="SV40 ori"  
     misc_feature    7997..8474 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4671..4678 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4671 
                     /label="Sf3b3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTTCTGTAC AACTTGACCT TACAGCGGGC CACTGGCATC AGCTTTGCCA 
     1081 TCCATGGCAA CTTCTCTGGA ACCAAACAAC AGGAAATTGT CGTTTCCCGT GGGAAGATTT 
     1141 TGGAGTTACT TCGCCCAGAT CCTAACACTG GCAAAGTGCA TACCCTTCTC ACTGTTGAAG 
     1201 TTTTTGGTGT TATCCGGTCA CTTATGGCAT TTAGGCTAAC AGGTGGCACC AAAGACTACA 
     1261 TTGTAGTTGG CAGTGACTCA GGCCGAATTG TTATCTTGGA ATACCAGCCA TCAAAGAATA 
     1321 TGTTTGAGAA AATTCACCAA GAAACTTTCG GCAAAAGTGG ATGTCGCCGT ATTGTTCCTG 
     1381 GCCAGTTCTT AGCCGTGGAT CCCAAAGGAA GAGCTGTTAT GATTAGTGCG ATTGAGAAGC 
     1441 AGAAATTGGT GTATATCTTG AACAGAGACG CTGCAGCCCG ACTTACCATT TCATCTCCCC 
     1501 TGGAGGCCCA CAAAGCAAAC ACTCTAGTGT ACCATGTGGT TGGAGTAGAT GTGGGATTTG 
     1561 AAAATCCTAT GTTTGCTTGT CTGGAAATGG ATTATGAGGA AGCAGACAAT GATCCAACAG 
     1621 GGGAAGCAGC AGCCAATACC CAGCAGACAC TTACTTTCTA TGAATTGGAC CTTGGTTTAA 
     1681 ATCACGTGGT TCGGAAATAC AGTGAACCGT TGGAGGAACA TGGCAACTTC CTTATTACAG 
     1741 TTCCTGGAGG GTCAGATGGT CCAAGTGGAG TGCTGATCTG CTCTGAGAAT TATATCACCT 
     1801 ACAAGAACTT TGGTGACCAG CCAGACATCC GATGTCCAAT TCCCAGGAGA CGGAATGACC 
     1861 TGGATGACCC TGAAAGAGGA ATGATTTTTG TTTGCTCTGC CACCCATAAG ACCAAGTCCA 
     1921 TGTTCTTCTT TTTGGCCCAA ACGGAGCAGG GAGATATCTT TAAGATCACT TTGGAGACAG 
     1981 ATGAAGATAT GGTAACTGAG ATCCGCCTCA AGTATTTTGA TACTGTTCCT GTTGCTGCTG 
     2041 CTATGTGTGT GCTTAAAACA GGCTTCCTTT TTGTGGCATC AGAATTTGGA AATCATTACT 
     2101 TGTATCAAAT TGCCCATCTT GGAGATGATG ATGAAGAGCC TGAGTTTTCA TCTGCCATGC 
     2161 CTTTAGAAGA AGGAGACACA TTCTTCTTTC AGCCGAGACC ACTCAAAAAT CTCGTGCTGG 
     2221 TGGATGAACT GGACAGCCTC TCTCCTATTC TCTTTTGCCA GATAGCTGAT TTGGCCAATG 
     2281 AAGACACTCC ACAGCTGTAT GTAGCCTGTG GTAGGGGACC CCGGTCGTCT CTGAGAGTCT 
     2341 TAAGACATGG ACTTGAGGTG TCGGAAATGG CTGTTTCTGA GCTCCCTGGT AACCCCAATG 
     2401 CTGTCTGGAC TGTCCGTCGG CACATTGAAG ATGAGTTTGA TGCCTACATC ATTGTGTCTT 
     2461 TCGTGAATGC CACGCTGGTG CTGTCCATCG GAGAGACTGT GGAAGAAGTG ACAGACTCCG 
     2521 GGTTCTTGGG TACCACTCCA ACCTTGTCCT GTTCCTTATT GGGAGATGAC GCGTTGGTGC 
     2581 AGGTGTATCC AGATGGCATT CGACACATCC GAGCAGACAA GAGAGTCAAT GAGTGGAAGA 
     2641 CTCCTGGCAA GAAGACAATT GTGAAGTGTG CAGTGAACCA GCGACAAGTG GTGATCGCCT 
     2701 TGACTGGGGG AGAGCTGGTC TATTTCGAGA TGGATCCTTC AGGACAGTTG AATGAGTACA 
     2761 CGGAGCGGAA AGAAATGTCA GCAGATGTGG TGTGCATGAG TCTGGCCAAC GTACCCCCTG 
     2821 GAGAACAGCG GTCTCGGTTC CTGGCTGTGG GGCTGGTGGA CAACACTGTC AGAATCATCT 
     2881 CCCTTGACCC ATCAGACTGT TTGCAGCCTC TGAGTATGCA GGCTCTCCCA GCCCAGCCTG 
     2941 AGTCTCTGTG TATTGTGGAA ATGGGTGGGA CCGAGAAACA AGATGAGCTG GGTGAGAGGG 
     3001 GCTCTATCGG CTTCCTGTAC CTGAACATTG GACTCCAGAA CGGTGTGCTG CTGAGGACTG 
     3061 TCCTAGACCC TGTTACTGGG GACCTGTCTG ACACCCGCAC TAGGTATCTG GGGTCCCGTC 
     3121 CTGTGAAGCT CTTTCGGGTT CGGATGCAAG GCCAGGAGGC TGTTCTGGCT ATGTCTAGTC 
     3181 GCTCATGGCT GAGCTACTCT TACCAGTCTC GATTCCATCT CACCCCTCTG TCTTATGAGA 
     3241 CCCTGGAATT TGCATCGGGC TTTGCCTCTG AACAGTGTCC TGAAGGCATC GTTGCCATCT 
     3301 CCACCAACAC TTTGAGGATT TTGGCACTAG AGAAGCTTGG CGCTGTCTTC AATCAAGTGG 
     3361 CCTTTCCTTT GCAGTACACA CCCAGGAAGT TTGTCATCCA CCCTGAGAGT AACAACCTTA 
     3421 TCATCATAGA GACGGATCAC AATGCCTACA CTGAGGCTAC AAAAGCTCAG AGAAAGCAGC 
     3481 AGATGGCTGA GGAAATGGTA GAAGCAGCAG GGGAGGATGA ACGGGAGCTG GCTGCAGAGA 
     3541 TGGCAGCCGC GTTCCTCAAT GAAAACCTCC CAGAATCCAT TTTCGGAGCT CCCAAGGCTG 
     3601 GCAATGGGCA GTGGGCCTCT GTGATTCGGG TAATGAACCC TATCCAGGGG AATACACTAG 
     3661 ACCTTGTCCA GCTGGAGCAG AATGAGGCAG CCTTTAGTGT GGCAGTGTGT AGGTTCTCCA 
     3721 ACACTGGTGA AGACTGGTAT GTGCTGGTTG GTGTTGCTAA GGACTTGATC CTGAGCCCTC 
     3781 GGTCTGTGGC AGGGGGCTTT GTGTATACAT ACAAGCTTGT GAACAATGGG GAAAAGTTGG 
     3841 AGTTTTTGCA CAAGACTCCA GTGGAAGAAG TCCCTGCTGC CATTGCCCCA TTCCAGGGGA 
     3901 GGGTACTGAT TGGTGTGGGG AAACTTTTAC GAGTCTATGA CCTGGGGAAG AAGAAGTTAC 
     3961 TTCGAAAGTG TGAGAATAAG CATATTGCCA ACTACATATC AGGGATCCAG ACAATTGGTC 
     4021 ACAGGGTAAT CGTGTCTGAT GTCCAGGAAA GTTTCATCTG GGTTCGCTAC AAGCGTAATG 
     4081 AGAACCAGCT CATTATCTTT GCTGATGACA CCTATCCCAG ATGGGTCACC ACTGCCAGCC 
     4141 TTCTCGACTA TGATACTGTG GCTGGGGCAG ACAAGTTTGG CAACATTTGT GTGGTGAGGC 
     4201 TCCCACCTAA CACCAACGAT GAGGTGGATG AAGATCCCAC TGGAAACAAA GCCCTGTGGG 
     4261 ACCGGGGCTT GCTCAATGGG GCTTCACAGA AGGCAGAGGT CATCATGAAC TACCACGTTG 
     4321 GGGAGACAGT GCTGTCGTTG CAGAAGACCA CACTGATCCC TGGAGGCTCA GAATCACTTG 
     4381 TCTATACCAC TTTATCTGGA GGAATTGGCA TCCTTGTCCC TTTCACGTCT CATGAGGACC 
     4441 ACGACTTTTT CCAGCATGTT GAGATGCACC TGCGGTCTGA GCATCCTCCT CTCTGTGGAC 
     4501 GGGACCACCT CAGCTTTCGT TCTTATTATT TCCCTGTTAA GAATGTGATT GATGGAGACC 
     4561 TTTGTGAGCA GTTCAACTCC ATGGAGCCCA ACAAGCAGAA AAATGTCTCT GAAGAGCTGG 
     4621 ACCGAACCCC ACCTGAAGTG TCCAAGAAGC TTGAGGATAT TCGGACCCGC TATGCTTTCA 
     4681 GCGGACCGAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4741 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4801 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4861 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4921 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4981 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     5041 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5101 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5161 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5221 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5281 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5341 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5401 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5461 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5521 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5581 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5641 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5701 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5761 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5821 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5881 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5941 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     6001 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6061 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6121 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6181 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6241 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6301 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6361 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6421 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6481 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6541 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6601 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6661 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6721 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6781 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6841 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6901 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6961 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     7021 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7081 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7141 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7201 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7261 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7321 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7381 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7441 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7501 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7561 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7621 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7681 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7741 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7801 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7861 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7921 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7981 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     8041 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8101 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8161 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8221 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8281 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8341 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8401 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8461 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8521 CAAAAATTTA ACGCGAATTT T
//