LOCUS       RR203572                8874 bp    DNA     circular  SYN 
DEFINITION  Ikbkap (Myc-DDK-tagged ORF) - Rat inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase complex-associated protein (Ikbkap), (10 ug)
ACCESSION   NM_080899
VERSION     NM_080899.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5031..5122 
                     /label="Myc-DDK"  
     misc_feature    5152..5737 
                     /label="PolyA signal"  
     misc_feature    5209..5228 
                     /label="Restriction Enzyme"  
     misc_feature    6013..6685 
                     /label="ColE1"  
     misc_feature    7115..7909 
                     /label="Kan/Neo"  
     misc_feature    7944..8246 
                     /label="SV40 ori"  
     misc_feature    8332..8809 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5013..5019 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5013 
                     /label="Ikbkap"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGAAATCTG AAGTTGCATC AGACCCTGGA ATTTAGGGAC ATTCAGGCAC 
     1081 CAGGGAAACC TCAGTGTTTC TGTCTGCGAG CTGAACAGGG GACAGTTCTG ATTGGCTCAG 
     1141 AACGTGGACT GACAGAAGTA GATCCTGTGA AAAAAGAAGT GAAGACTGAA ATTTCTTTGG 
     1201 TGGCAGAAGG TTTTCTTCCA GAGGATGGAA GTGGCTGCAT TGTGGGTATT CAGGACTTGC 
     1261 TGGATCAAGA ATCTGTGTGT GTGGCCACTG CCTCTGGGGA CGTCATAGTC TGCAATGTCA 
     1321 GCACACAGCA GCTGGAATGT GTTGGGAATG TGGCCAGTGG TATCTCTGTC ATGAGCTGGA 
     1381 GTCCTGATCA AGAACTGCTG CTGCTTGCCA CAGCTCAACA GACCCTGATT ATGATGACAA 
     1441 GAGACTATGA AGTAATCACA GAGCAGCAGA TCCACCAGGA TGATTTTGGA GAAGGCAAGT 
     1501 TTATCACCGT TGGATGGGGC AGTAAGGACA CGCAGTTCCA CGGCTCAGAA GGCAGGCCAA 
     1561 TAACCTTTCC GGTGCAAATG CATGAGTCTG CCTTGTCCTG GGATGACCAC AGACCACAAA 
     1621 TTACCTGGCG TGGGGATGGG CAATTTTTTG CTGTGAGTGT TGTTTGCTCA CAAACAGGGG 
     1681 CTCGGAAAAT CCGAGTATGG AACCGAGAGT TTGCCTTGCA GTCAACCAGT GAGTCTGTGC 
     1741 CAGGGCTGGG GCCCTCCCTG GCTTGGAAAC CCTCAGGCAG TCTGATTGCA TCTACTCAGG 
     1801 ATAAACCCAA CCAGCAAGAC GTTGTGTTTT TTGAGAAAAA TGGACTTCTT CATGGGTATT 
     1861 TTACACTTCC TTTCTTGAAA GATGAAGTTA AGGTAAATGA CTTGCTGTGG AATGCAGATT 
     1921 CCTCGGTGCT TGCTGTGTGG CTAGAAGACC TCCCGAAGGA AGGCAGTTCC ACTCTGAAAA 
     1981 GCTATGTCCA GCTCTGGACC GTGGGGAACT ATCACTGGTA TCTCAAGCAA AGCCTGCCCT 
     2041 TCAGCACCAC GGGGAAGAAC CAGATCGTGT CTCTGCTGTG GGACCCAGTG ACGCCGGGCC 
     2101 GACTGCATGT TCTCTGTCAG GGCTGGCGGT ATCTATGCTG TGACTGGCAC TGGACCACTG 
     2161 ATCGGAGCTC TGGAAATAGT GCCGATGACC TGGCAAATGT GGCTGTCATT GATGGAAACA 
     2221 AGGTGTTGGT AACAGTCTTC CAGCGGACTG TGACCCCACC TCCCATGTGT ACCTACCGGC 
     2281 TGCTGATCCC ACATCCAGTG AATCAAGTCA TGTCCTCTGC TCACCTTGGA AATGACCTTG 
     2341 CTGTCCTTGA CGCCAGTAAC CAGATTTCTG TCTACAAGTG TGATGATAAA CCAGACATGG 
     2401 ATTCCACAGT GAAACTAGGA GCTGTGGGTG GAACTGGATT TAAAGTTCCT CTTAGAACAC 
     2461 CTCATCTGGA AAAAAGATAC AGGATTCAGT TTGGGAACAA GGAAGAGGAG GAGGATGTAA 
     2521 GCCCACTGCA ATTTCGATTT CTCACCTGGA TTGAAGGTGA CGCTTTCCTG GCCATAAGCC 
     2581 ACAGTCACTC CAGCCCACAG TCCATCATTC ACCATCTGAC TATGGCCGGT TCTGAGGGGG 
     2641 ATGAGGAACA AGGACAGCTG AATGTCAGTT CATCTGTGAC AGTGGATGGA GTTGTCATTG 
     2701 GTTTATGTTG CTGTTCTAAG ACCAAGTCAT CAGCAGTACA GCTGGCTGAC GGCCAGGTGC 
     2761 TCAAGTACCT TTGGGAGTCG CCTTCTTCGG CTGTGGAGCC CTGGAAGAAC TCTGAAGGGC 
     2821 GTCCTGTCCG ATTTGCTCGT CCATGCACCC AGATGGAAGC AGCCGCCATT GGAGGGGAGG 
     2881 AATGTGTCCT TGGTCTGACC GACAGGTGTC GCTTTTTCAT CAACGACACT GAGGTTGCAT 
     2941 CAAATATCAC GTCATTTGCA GTGTGTGATG ACTTTCTACT GGTGACAACC CATTCCCACA 
     3001 CCTGCCAGTG TTTCTCTCTA AGTGGTGCCT CACTTAAAAT GCTGCAGGCA GGTCTGTGTG 
     3061 GCAGTCAGGT GCCCAGTGGA GAGATTCTGA GGAAGGTGGA GAGGGGTTCC CGGATCGTCA 
     3121 CAGTCGTCCC CCAGGACACA AAGCTTATAC TCCAGATGCC GAGGGGGAAC TTGGAGGTTG 
     3181 TTCATCATCG GGCCTTGGTC TTGGCTCAGA TTCGGAAGTG GCTGGACAAA CTTATGTTTA 
     3241 AAGAAGCGTT TGAATGCATG AGGAAACTGA GAATTAACCT CAATCTGATT CATGATCATA 
     3301 ACCCCAAGGT GTTTCTTGAA AATGTGGAAA CCTTCATAAA ACAGATTGAT TCTGTAAATC 
     3361 ATCTAAACTT GTTTTTCACA GAACTAAAGG AAGAAGATGT CACAAAGACC ATGTACCCTC 
     3421 CCCCGGTTAC CAAGAGTGTC CAGGTGTCCA CAAATCCTGA TGGAAAGAAA GTTGACCTCA 
     3481 TCTGTGACGC TATGAGAGTG GCCATGGAGA CCATCAATCC TCGCAAGTTC TGCCTGTCCA 
     3541 TACTCACATC TCATGTGAAG AAGACGACTC CAGAACTGGA CATTGTGCTG CAGAAGGTAC 
     3601 ACGAACTTCA AGGAAAAATT CCCTTTGTTC CTGAGAGTGT GAGTGCAGAG GAGGCTTTGA 
     3661 AATACCTGCT GCTCCTGGTA GATGTTAATG AATTATTTAA TCATTCTCTT GGGACCTATG 
     3721 ACTTTGATTT GGTCCTCATG GTAGCTGAGA AGTCACAAAA GGATCCTAAG GAATATCTTC 
     3781 CATTTCTTAA TACACTTAAG AAAATGGAAA CTAATTACCA ACGGTTCACC ATAGACAAGT 
     3841 ACTTGAAACG ATATGAAAAG GCCCTTGGAC ACCTCAGCAA GTGTGGACCT GAGTATTTCA 
     3901 CAGAATGCTT AAATTTGATA AAAGATAAAA ACTTGTATAA AGAAGCTCTG AAGTTATATC 
     3961 GGCCAGACTC ACCACAGTAC CAGGCGGTCA GTGTTGCTTA TGGCGAGCAC CTGGTGCAGG 
     4021 AGCTCCTGTA CGAGCCAGCA GGGCTTGTGT TTGCCCGCTG TGGGGCCCAT GAGAAGGCTC 
     4081 TGGAAGCGTT TTTGGCTTGT GGCAGCTGGC AGCAAGCCCT TTGTATGGCT GCCCAGCTTC 
     4141 AGATGGCCAA AGACAAGGTG GCTGGCCTTG CTAGAACTCT GGCAGGAAAG CTGGTCGAGC 
     4201 AGAGGAAACA CAGTGAGGCA GCAACAGTCC TGGAACAGTA CGCCCTGGAT TATGAAGAAG 
     4261 CCGTGCTCTT GCTGCTAGAA GGATCTGCCT GGGAAGAAGC TCTGAGGCTG GTATACAAGT 
     4321 ATGACAGAGT GGATATTATA GAAACCAGCG TAAAGCCTTC CATTTTAGAA GCCCAAAAAA 
     4381 ATTACATGGA CTTTCTGGAC TCTCAGACAG CCACATTCAT TCGCCATAAA AACCGCTTGA 
     4441 AGGTGGTTCG AGAGCTCAAG AGCCAACGAC CACGGGTGCA TGTGGATCAC GAGGTGGCCC 
     4501 ATGGCCGAGA GACGGACCTC TTCTCGGAAA CCAGCAGTAT TAGGAGTGGC AGCGAGATGA 
     4561 GTGGCAGATA CTCCCACAGT AACTCCAGGA TATCTGCGAG GTCATCTAAA AACCGCCGCA 
     4621 AGGCAGAACG GAAGAAGCAT AGCCTCAAAG AAGGGAGCCC CCTGGAGGGC CTGGCACTTC 
     4681 TGGAGGCGCT GAGTGAGGTG GTACAGAGCA TTGAGAAGCT GAAAGATGAA GTGCACGCTA 
     4741 TTTTAAAGGT GCTCTTTCTC TTTGAGTTCG AAGAGCAAGC AAGGGAGTTA CAGAGGGCCT 
     4801 TTGAGAGTAC TCTGCAGCTG ATGGGGACGG CACTTCCAGA CATCTGGACT CTCACCGGCC 
     4861 AACAGAGTTC ATCCACACCT GTCCTAGGTC CCAGTTCCAC TGTGAATAGT ATCATGGCTT 
     4921 CTTACCAGCA GCAGAAGACT TGTGTCCCTG TTCTCGATGC TTGGGCTTGT TTGCCACCGA 
     4981 AGATCGACCA GAGAAGCCAG TGGAAGCTGA GCCTGCTGGA AACGCGTACG CGGCCGCTCG 
     5041 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     5101 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     5161 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     5221 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     5281 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5341 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5401 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5461 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5521 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5581 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5641 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5701 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5761 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5821 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5881 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5941 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     6001 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     6061 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     6121 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     6181 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     6241 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6301 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6361 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6421 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6481 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6541 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6601 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6661 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6721 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6781 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6841 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6901 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6961 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     7021 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     7081 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     7141 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     7201 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     7261 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7321 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7381 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7441 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7501 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7561 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7621 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7681 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7741 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7801 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7861 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7921 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7981 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     8041 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     8101 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8161 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     8221 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     8281 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8341 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8401 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8461 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8521 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8581 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8641 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8701 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8761 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8821 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//