LOCUS       RR203565                8325 bp    DNA     circular  SYN 
DEFINITION  Man2a1 (Myc-DDK-tagged ORF) - Rat mannosidase, alpha, class 2A, member 1 (Man2a1), (10 ug)
ACCESSION   NM_012979
VERSION     NM_012979.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4482..4573 
                     /label="Myc-DDK"  
     misc_feature    4603..5188 
                     /label="PolyA signal"  
     misc_feature    4660..4679 
                     /label="Restriction Enzyme"  
     misc_feature    5464..6136 
                     /label="ColE1"  
     misc_feature    6566..7360 
                     /label="Kan/Neo"  
     misc_feature    7395..7697 
                     /label="SV40 ori"  
     misc_feature    7783..8260 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4464..4470 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4464 
                     /label="Man2a1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGTTAAGC CGCCAGTTCA CCGTGTTCGG CAGCGCGATC TTCTGCGTCG 
     1081 TAATCTTCTC ACTCTACCTG ATGCTGGACA GGGGTCACTT GGACTACCCT CGGGGCCCGC 
     1141 GCCAGGAGGG CTCCTTCCCG CAGGGCCAGC TTTCAATATT GCAAGAAAAG ATCGACCATT 
     1201 TGGAGCGTTT GCTAGCGGAG AACAACGAGA TCATCTCGAA TATCAGAGAC TCGGTCATCA 
     1261 ACCTGAGTGA GTCTGTGGAA GATGGCCCAC GAGGTCCAGC AGGCAACGCC AGCCAAGGCT 
     1321 CTGCGCACCT CCACTCTGCA CAGTTGGCCC TACAGGCTGA CCCCAAAGAC TGCTTGTTTG 
     1381 CCTCGCAGAG CGGAAACCAG CACCGGGATG TGCAGATGTT GGATGTTTAC GATCTGATTC 
     1441 CTTTTGATAA TCCAGATGGT GGAGTTTGGA AGCAAGGGTT TGACATTAAG TACGAAGCTG 
     1501 ATGAGTGGGA CCGTGAGCCC CTGCAAGTGT TTGTGGTGCC TCACTCCCAT AATGACCCAG 
     1561 GTTGGTTGAA GACTTTCAAT GACTACTTTA GAGACAAGAC TCAGTATATT TTTAATAACA 
     1621 TGGTCCTAAA GCTGAAAGAA GACTCAAGCA GGAAGTTTAT ATGGTCTGAG ATCTCTTACC 
     1681 TTGCAAAATG GTGGGATATT ATAGATAATC CGAAGAAGGA AGCTGTTAAA AGTTTACTAC 
     1741 AGAATGGTCA GCTTGAGATT GTGACCGGTG GCTGGGTGAT GGCCGATGAA GCCACTACAC 
     1801 ATTATTTTGC CTTAATTGAC CAACTGATTG AAGGGCATCA ATGGCTGGAA AAAAATCTAG 
     1861 GAGTGAAACC TCGATCGGGC TGGGCCATAG ATCCCTTTGG ACATTCACCC ACAATGACTT 
     1921 ACCTTCTAAA ACGTGCTGGA TTTTCACACA TGCTCATCCA GAGAGTCCAT TATTCGGTGA 
     1981 AAAAACACTT CTCTTTGCAA AAAACGCTGG AATTTTTCTG GAGACAGAAT TGGGATCTTG 
     2041 GATCCACCAC AGACATTTTG TGCCACATGA TGCCCTTCTA TAGCTATGAC ATCCCCCACA 
     2101 CCTGTGGACC CGATCCCAAA ATATGCTGCC AGTTTGATTT TAAGCGGCTT CCTGGAGGCA 
     2161 GATACGGTTG TCCCTGGGGA GTTCCCCCAG AAGCAATATC TCCTGGAAAT GTCCAAAGCA 
     2221 GGGCTCAGAT GCTACTGGAT CAGTACCGGA AAAAGTCAAA ACTTTTCCGC ACCAAAGTTC 
     2281 TCCTGGCTCC GCTCGGAGAC GATTTTCGGT TCAGTGAATA CACAGAGTGG GACCTGCAGT 
     2341 ACAGGAACTA TGAGCAGTTA TTCAGTTACA TGAACTCTCA GCCCCATCTT AAAGTGAAGA 
     2401 TCCAGTTTGG AACTTTGTCA GATTATTTCG ATGCATTGGA GAAGTCTGTG GCAGCTGAGA 
     2461 AGAAGGGTGG CCAGTCTGTG TTCCCCGCCC TGAGTGGAGA CTTCTTCACC TACGCTGACC 
     2521 GAGACGACCA TTACTGGAGT GGCTACTTCA CATCCAGACC TTTCTACAAA CGAATGGACA 
     2581 GGATAATGGA ATCTCGTTTA AGGACTGCTG AAATTCTTTA CCATTTGGCC TTGAAACAAG 
     2641 CTCAGAAATA CAAGATAAAT AAATTTCTTT CATCACCTCA TTATACAACA CTGACAGAAG 
     2701 CCAGAAGGAA CTTGGGACTG TTTCAGCATC ATGATGCCAT CACGGGAACC GCGAAAGACT 
     2761 GGGTGGTCGT GGACTATGGT ACCAGACTCT TTCAGTCATT AAATTCTCTG GAGAAGATAA 
     2821 TCGGAGATTC TGCATTTCTT CTCATTTTAA AGGACAAAAA GCTGTACCAG TCAGATCCTT 
     2881 CCAAAGCCTT CTTAGAGATG GACACGAAGC AAAGTTCACA AGATTCTCTG CCCAAAAAAA 
     2941 ATATAATACA ACTGAGTGCA CAGGAGCCGA GGTACCTCGT GGTCTACAAT CCCTTCGAAC 
     3001 AAGAACGGCA CTCGGTGGTG TCCGTCCGTG TGAACTCAGC CACGGTGAAA GTGCTGTCTG 
     3061 ATCTGGGGAA AGCCGTGGAG GTTCAAGTCA GCGCAGTCTG GAAGGATATG AGAACGACTT 
     3121 CACAAGCAGC CTATGAGGTC GCTTTTCTAG CTCATCTGCC ACCGCTGGGA CTGAAAGTGT 
     3181 ACAAGATCTT AGAATCCCAG AGTTCAAGTT CACATCTGGC TGATTATTTC CTATATAACA 
     3241 ATGACGGGCA AGCAGAAAGT GGAATATTCC ACATGAAGAA CATGGTAGAT TCAGGAGATG 
     3301 CCATCACCAT AGAGAATTCC TTCCTCACAC TTGGGTTTGA CCGATCTGGG CTGATGGAGA 
     3361 AAGTGAGAAG GAAAGAAGAC AATAAACAGC AGGAACTGAA GGTGCAGTTC CTGTGGTACG 
     3421 GAACCACCAA CAAAAGGGAC AAGAGCGGTG CCTACCTCTT CCTGCCTGAT GGGCAGGGCC 
     3481 AGCCCTATGT TTCCCTGAGA ACACCCTTTG TCAGAGTGAC ACGTGGAAGG ATCTACTCAG 
     3541 ACGTGACCTG CTTCCTTGAA CACGTTACTC ACAAGGTCCG GCTGTACCAC ATACAGGGAA 
     3601 TAGAAGGCCA GTCCATGGAG GTTTCTAATA TCGTGGACAT CAGGAGTGTG CATAACCGTG 
     3661 AGATTGTAAT GAGAATTTCC TCCAAAATAA ACAACCAAAA TAGATATTAT ACCGACCTAA 
     3721 ATGGATATCA GATTCAACCT AGAAGGACAA TGGCCAAACT GCCTCTTCAG GCCAATGTGT 
     3781 ACCCGATGAG CACAATGGCC TACATCCAGG ATGCTGCACA TCGGCTCACA CTGCTTTCCG 
     3841 CTCAGTCTTT AGGCGCTTCC AGTATGGCCT CTGGTCAAAT TGAAGTCTTC ATGGACCGAA 
     3901 GACTCATGCA GGACGATAAC CGTGGCCTTG GGCAAGGTGT ACACGATAAT AAGATTACAG 
     3961 CCAATTTGTT TCGAATCCTG CTAGAGAAGA GAAATGGCAT GAACATGGAA GAGGACAAGA 
     4021 AGAGCCCTGT CAGCTACCCT TCCCTCCTCA GCCATATGAC CTCGGCCTTC CTCAACCATC 
     4081 CCTTCCTCCC CATGGTACTA AGTGGCCAGC TCCCCTCCCC TGCCATTGAG CTGCTGAGTG 
     4141 AATTTCGTCT GCTGCAGTCC TCTCTCCCTT GTGATATCCA TCTGGTTAAT CTACGGACAA 
     4201 TACAGTCAAA GGTGGGCAAA GGTTACTCAG ATGAGGCAGC CTTGATCCTC CACAGGAAGG 
     4261 TGTTTGATTG CCAGTTGTCC AGCAGAGCCA TGGGGCTGCC CTGTTCCACG ACTCAGGGAA 
     4321 AGATGTCAAT TCCAAAACTT TTCAACAACT TTGCTGTGGA GAGTTTCATC CCTTCCTCGC 
     4381 TGTCCTTGAT GCACTCGCCT CCGGACGCCC AGAACACGAG TGAAGTCAGC CTGAGTCCCA 
     4441 TGGAGATTAG CACGTCCCGA ATCCGCTTGA GGACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4501 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4561 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4621 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4681 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4741 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4801 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4861 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4921 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4981 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5041 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5101 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5161 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5221 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5281 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5341 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5401 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5461 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5521 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5581 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5641 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5701 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5761 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5821 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5881 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     5941 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6001 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6061 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6121 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6181 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6241 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6301 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6361 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6421 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6481 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6541 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6601 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6661 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6721 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6781 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6841 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6901 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     6961 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7021 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7081 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7141 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7201 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7261 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7321 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7381 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7441 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7501 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7561 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7621 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7681 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7741 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7801 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7861 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7921 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     7981 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8041 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8101 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8161 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8221 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8281 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
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