LOCUS       RR203486                8802 bp    DNA     circular  SYN 
DEFINITION  Cenpj (Myc-DDK-tagged ORF) - Rat centromere protein J (Cenpj), (10 ug)
ACCESSION   NM_001107265
VERSION     NM_001107265.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4959..5050 
                     /label="Myc-DDK"  
     misc_feature    5080..5665 
                     /label="PolyA signal"  
     misc_feature    5137..5156 
                     /label="Restriction Enzyme"  
     misc_feature    5941..6613 
                     /label="ColE1"  
     misc_feature    7043..7837 
                     /label="Kan/Neo"  
     misc_feature    7872..8174 
                     /label="SV40 ori"  
     misc_feature    8260..8737 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4941..4947 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4941 
                     /label="Cenpj"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAAATGTTC CTGATGCCAA CCTCTTCAGA ATTAAACACT GGGCAGAACT 
     1081 TCCTCACCCA GTGGATGACT AGTCCTTCTC GGGCTGGGGT CATATTAAAT CGAGAATTTC 
     1141 CTATTTTGGA TGCCGATGAG AAGCAAGCAG CTACAACTGT TTCTGCCAGC TTTCCCGTGA 
     1201 AAGCCGCCCA TTTTTCAAGT AGCTTCAGTA TTAGTAGTGA AGAGGATTCA TTTCATGAAG 
     1261 AACAGACGCT GGAGGCTGGA AGACCTTATA AACCACGGTC AGAAGACCCG GAAGCTCATC 
     1321 CAGTCTCTGC TTCAGTCAGA AGGGAGCCAA TAGCATCATG TCAGGATGCC CCAGGATGCC 
     1381 AGGAGGGTAA CAGAAATGCC GCTACGCCTG ATCTCAAGAG TGAATTCAAA GAAGTGGCTA 
     1441 ATAAAAACCC ACTTTTTAAA AAACTGGAGC AGCTGAGCCA GAAGCATCGC TCTCCAGGAG 
     1501 ACTCAGCCTC AGGAGAGAGG GCCACCCTGT CACCACCCAG CTACCCAAAT CCAACCCAAG 
     1561 ACAACTCCCG TGCTTCCAGC CTTCTACCAG ATGAACAAAG TAACTTCTGT AGAGCCACTC 
     1621 AACAAGATTC TGTTTTAACT TCAAAAAATG CTTCTGATTT GTTTCATGAG TCACAATATC 
     1681 ATGAAGCACA TGTGAGAAGA AATGATTTAA AGGAGGAAAG CCATAACCAT CCTTCAGGGG 
     1741 AAGGTGCTTT GCCATGCTGG GAAAAGAAGA TGGGGAAGAG CCAGGAAGGG AAGGATGTGA 
     1801 ACTTACAAAG AAGTAGAGAT TCCTCAGGAG TGGTTAATAT TGAAGAGAGG CCAATCAAAG 
     1861 CTGCCATTAG AGAGAAGCAG CAGACATTCG AGGACTACTT AGAGGAGCAG ATCCGACTGG 
     1921 AAGAGCGAGA ACTGAGACAG AAGCAGCTGC AGGAAGCAGA AGGACCATTA CTAACGAAAA 
     1981 TGAAACCCAA GCAGCCATTT TTAAAACGAG GAGAAGGTTT AGCGAGGTTT ACTAATGCTA 
     2041 AATGTAAATT TCAGAAGGGG AAAGAAAGCA AGCTAGCGAG TACCCAGAGC CCGTCAGAGC 
     2101 AGCAGTCTGT GCCTAAAGTA GACAGACAGC ACTTGCAGAG GAAAACCGCG CTCATAAACA 
     2161 GGGACCTGTG TGCAGACACC ACCTCTGTTA AAAAGGACAG TAAAGCTAGG TCCAAGGCTG 
     2221 GCTCGGCCTC ACTCAGGCAG AAACCCAAAG TGACCAAGAC CAATAGGAGG GACAGTCTAT 
     2281 CCCCGCCGGG ACGGAAAGTA CAGACGGGGA AGAAATGTGA TGGACAATTT AGACCCCAGG 
     2341 TCAAATTAGA GAGAAATGTG CCCACCAATA ATAAGGAAAA TGTACCTGCG TACATAAAGC 
     2401 CCTGGGGTGC TGGCTGCAAG GTGTGGAATA AGACACAGGG GAGAGACAGA CTTCCTCTTT 
     2461 CCACAGGGCC GGTTGGCTGT GTGGTCTCCA GGAGTTTGGT ACCTGAGACA GATAGACAGA 
     2521 CAGAATCTTC CCTTGATATT TCTCTTCAGA AAAAATTAGA GAATTGGGAG AGAGAAAAAG 
     2581 AAAAGGAAAA CTTGGAACTA GATGAATTTT TATTTTTGGA ACGGGCTGCT GATGAAATAT 
     2641 CATTTTCTAG TAATTCCTCT TTTGTTCTGA AAATCTTAGA AAGGGACCAA CAGATCTATG 
     2701 AAGGTCACAG GCTGTCTTCC ACCCCTGTCA AAGCTGTGCA GCCCCAGAAG GCACAGCAGG 
     2761 CAGATCCTTG GGGGCAGAGT AACCACAGTG AGGTTCCCCG CCCCAGTGTT GCACATGAGG 
     2821 ATGAGAGTGA GTTTGAGGCC ATGCTCTTGT CTCGGGGTTC AGGGTCACCT GGCTTGAGGG 
     2881 AACTGTCCTG TAAACTTAGT AAGAAAGCCT TCCAAAACAG CACTTCTGAG AACCAGGGCC 
     2941 GATGGGATGC AGGGGATGGT GACGTTGCCA ACAGCGACAG TAGCTCTGAG TCTGAGGAGC 
     3001 ATGACATTAC CATCAAACCG TCCACCGAGG CTGAGGACAG GGTCTTCAGC AACAGAGAGG 
     3061 ATAGTCCACA AGTCTGTGAC GCCAAAGGAC CTTCTCGGGA CACCGGGGCT CAAGAAGATC 
     3121 AGTGGAGAGA CGCAGACCTG GATCTGTCTG ATAAAGAGTG TAGCAGTGAC GAGTCTGTCA 
     3181 CAGTTGAAAG TCTGAGCAGT CAAGTATTGG AGCCGCTGAG ACTGCCCTCC TCTCACGACG 
     3241 GGAGTAAAGT CGACTTCGAT GATGAAAGAT CTTGGGCAGA CCTTGAGGAG AATCCACATG 
     3301 AACATGGTGT TGTTCCCAGA GAGGAAGCCA TGTCTGGTAC TTCCCAGACA CACTGCCACA 
     3361 GTACAAGTGA AGGATGTGTT CTGGACAAAA CAATAAAAAG GAAGATTGCA CCAGTCAAGA 
     3421 AGGGAGAAGA CTTCAGCAAG TCTGACAGGA GCGTAAGTCC TCCTCCATCT GACTTGATGG 
     3481 TAAAATTCTT CCCTTCTTTG AAACCAAAGC CAAAATTAGA TTCACACGTA GAAAATGAAC 
     3541 CCAAGTTAAG CCTGAGTCAA GATCAACCAC CAGGTGACAG TGTTCGATCT CAGGTCTTAA 
     3601 GAGAGAAAGT TATTGAACTG GAATCAGAAA TAGAAAAATT TAAAGCTGAG AACACATCTT 
     3661 TAGCTAAACT TCGTATTGAA CGAGAAAGTG CCTTGGAAAA ACTGAGGAAA GAAATTGCTG 
     3721 ACTTTGAGCA ACAAAAAGCA AGAGAACTGG CTCGAATAGA AGAGTATAAA AAGGAGGAGA 
     3781 CGAGGAAACT ACAAAAGGAG CGCAAAGTCT TCGAGAAGTA CACAGCAGCT GCAAGAACAT 
     3841 TCCCAGATAA AAAGGAGCGG GAAGAAATAC AGGCTTTGAA ACAGCAAATA CAGGAATTAC 
     3901 AGGAAGACTT GAAAAGGAAG GAAACAAAAT GGTCAAGTAC ACACGGTCGC CTCAGAAGCC 
     3961 AGATAGAAAT GCTAGTCAAA GAGAATACAG ACCTCCGAGA AGAAATAAAA GTAATGGAAA 
     4021 GATTCCGACT GGAGGCCTGG AAGAGAGCAG AAGCTATTGA GAACAGTCCC AAGGCCTGCC 
     4081 AGTACATAAT GGCCACAAAA AAGGATGAGT CCATGAACTC ATCAATTCAA TTTCAAAAGA 
     4141 GTCATGCTTC TTTAGGAGTC CAGGGAGAAA AATATAAGAA AAAGTATTTG CCAGCACAAG 
     4201 GAAATTCATC TAGAAGATCC AAGTCTGTGC CGCCTCGTGA TTTAGGCAGC TTGGATAAAG 
     4261 GACAAGCTGC CTTGCCTAGA GAGCCTCTTC AACCAGTGAA CTTCCCAGAC CTTGAATATA 
     4321 AAAACAAAGA GGAAAAAGAG GAAGAAATTC AGGGAGAAAT CAATCATCCT GATGGGAAGG 
     4381 TAGAAAAAGT TTATAAAAAT GGATGCCGGG TTGTTCTGTT TCCCAATGGA ACTCGGAAAG 
     4441 AAGTGAGTGC GGACGGGAAG AGCATCACGG TTACATTCTT CAATGGTGAC GTCAAACAGG 
     4501 TCATGCCAGA TGAGAGAGTG GTCTACTACT ATGCTGCTGC CCAGACCACA CATACCACGT 
     4561 ACCCAGAAGG GCTTGAAGTC TTACATTTCT CAAGCGGGCA AATAGAAAAT ATATTGATAA 
     4621 CAATCATTTT TAAAACAGAA AAGCATTTCC CAGATGGAAG AAAAGAAATC ACATTTCCTG 
     4681 ACCAGACCAT TAAGACTTTA TTTGCTGATG GTCAAGAAGA GAGCATCTTT CCAGATGGCA 
     4741 CAATTGTCCG AGTACAACGT GATGGCAACA AAATCATAGA GTTTAATAAT GGCCAAAGAG 
     4801 AGCTCCACAC TGCCCAGTTC AAGAGACGAG AATACCCCGA TGGGACTGTT AAGACTGTCT 
     4861 ATGCAAATGG TCATCAGGAG ACAAAGTACA CCTCTGGCCG AGTCAGAGTG AAGGACAAAG 
     4921 ATGGTAATGT GCTAATGGAC ACAGAAATGA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4981 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     5041 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     5101 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     5161 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     5221 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     5281 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     5341 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     5401 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5461 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5521 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5581 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5641 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5701 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5761 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5821 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5881 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5941 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     6001 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     6061 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     6121 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     6181 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     6241 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     6301 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     6361 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6421 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6481 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6541 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6601 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6661 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6721 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6781 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6841 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6901 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6961 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     7021 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     7081 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     7141 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     7201 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     7261 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     7321 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     7381 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     7441 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7501 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7561 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7621 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7681 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7741 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7801 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7861 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7921 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7981 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     8041 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8101 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8161 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     8221 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     8281 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     8341 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     8401 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     8461 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8521 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8581 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8641 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8701 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8761 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//