LOCUS       RR203451                7851 bp    DNA     circular  SYN 
DEFINITION  Bnc1 (Myc-DDK-tagged ORF) - Rat basonuclin 1 (Bnc1), (10 ug)
ACCESSION   NM_001108916
VERSION     NM_001108916.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4008..4099 
                     /label="Myc-DDK"  
     misc_feature    4129..4714 
                     /label="PolyA signal"  
     misc_feature    4186..4205 
                     /label="Restriction Enzyme"  
     misc_feature    4990..5662 
                     /label="ColE1"  
     misc_feature    6092..6886 
                     /label="Kan/Neo"  
     misc_feature    6921..7223 
                     /label="SV40 ori"  
     misc_feature    7309..7786 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3990..3996 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3990 
                     /label="Bnc1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGGCGGTCG CCGAGCCGGG GCGGACGCGG AGCGGCCCGG GCAGGGTACG 
     1081 CGCGCCGGGA AGGCCGGCCC CGGAGCGGCC GCAGGATGGC TGAGGCTATC GGCTGTACTC 
     1141 TGAACTGCAG TTGCCAAAGC TTCAAACCTG GGAAGATAAA CCACCGCCAG TGTGAACAGT 
     1201 GCAGGCATGG ATGGGTGGCT CATGCTCTAA GTAAGCTGAG GATTCCCCCC GTGTATCCGA 
     1261 CAAGCCAAGT GGAAATTGTC CAGTCCAATG TAGTGTTTGA TATTAGCAGC CTTATGCTCT 
     1321 ATGGAACCCA AGCTATCCCT GTTCGCCTGA AAATCCTACT GGACCGGCTC TTCAGTGTGC 
     1381 TGAAGCAAGA TGAGGTTCTA CAGATCCTCC ATGCCCTGGA CTGGACACTT CAGGATTACA 
     1441 TCCGTGGATA TGTGTTACAG GATGCCTCAG GGAAGGTGTT GGATCACTGG AGCATCATGA 
     1501 CCAGTGAGGA GGAAGTGGCC ACCTTGCAGC AGTTCCTTCG TTTTGGAGAG ACCAAGTCTA 
     1561 TAGTTGAGCT CATGGCAATC CAAGAGAAAG AAGAGCAGTC TATCGTCATT CCACCTGCCA 
     1621 CAGCAAACGT AGACATCAGG GCTTTCATCG AGAGCTGCGG CCACAGGAGT GCCAACCTCC 
     1681 CCACTCCGGT GGACAAAGGA AACCCCAGCA GCATGCATCC TTTTGAGAAC CTTATAAGCA 
     1741 ACATGACGTT CATGTTGCCT TTCCAGTTCT TCAACCCTCT GCCTCCTGCT CTGATAGGGT 
     1801 CGCTGCCTGA ACAGTACGTG CTGGAGCAGG GTCAGGACCA AAGCCGGGAC CCCAAGCAGG 
     1861 ACCTTCATGG GCCCTTTTCT GACAGTGGCT TCTTAACTTC CACCCCGTTT CAGGTTGAAA 
     1921 AGGAACAGTG TCTAAGCTGT CCAGACGCTG TCACCCAGAA GGAAGACAGT GCCCACTTAA 
     1981 GTGACTCCAG CTCCTACAGC ATTGCCAGTA AGCTCGAAAG GACACAGCTG TCCCCTGAGG 
     2041 CCAAAATGAA GCCTGAGAGG AACAGCCTGA GTGCTAAGAA GGGCCGGGTG TTCTGCACCG 
     2101 CATGCGAGAA GACATTCTAT GACAAAGGCA CTCTCAAGAT CCACTACAAC GCCGTCCACC 
     2161 TGAAGATCAA GCATAAGTGC ACTATCGAAG GGTGTAACAT GGTGTTCAGC TCCCTGAGGA 
     2221 GCCGGAACCG ACACAGCGCC AACCCCAACC CTCGCCTGCA CATGCCAATG AACAGAAACA 
     2281 ACCGAGACAA AGATCTCAGG AACAGCCTGA ACCTGGCAAG CTCCGAGACC TACAAGCGCC 
     2341 CAGGTTTCAC AGTGATGTCT CCAGACTGTG GGCCCCTCCC TGGCTACACT GGTTCAGTGG 
     2401 AGGACTCCAA AGGCCAACCA GCTTTCTCAA GCATTGGGCA AAATGGTGTG CTTTTCCCCA 
     2461 ACCTAAAGAC TGTCCAGCCA GTCCTTCCTT TCTACCGCAG TCCAGCTACT CCCGCGGAAC 
     2521 TGGCAAACAC ACCCGGCATG CTGCCTTCTC TCCCTCTGAT CTCCTCCTCA ATCCCAGAAC 
     2581 AGCTGGTTTC CACAGACGTG CCATTTGATG CTCTTCCCAA GAAGAAGTCC CGGAAGTCCA 
     2641 GTATGCCCAT CAAGATAGAG AAAGAAGCTG TGGAAATAGC TGATGAGAAG AGACACAGTC 
     2701 TCAGCTCAGA TGATGACGTG CCCCTGCAGG TAGTCAGTGA AGATGAGCCA GAGGACAGCA 
     2761 GTCCTCAGTC AGACAGAGTG CCTGAGGAGC AGCGTACACA GCTAGGCTTA GAGGAGCCTC 
     2821 TTCCACAGGG AGAAAGAGCC TGCCATCTTG AATCTGTGAT TGAGCCGCAT GGAGCTATCA 
     2881 GCAGAACCCT TGAGCAGACC ACGCTCACTG AGAGGGAGTC TGAACAGAAG GTCGCACTGA 
     2941 GTTCAGTGAT GCCAAGAGAG GTGGAGCATG ATGGTCACGA GCGACATTTC ACCCCTGGCC 
     3001 TGGGGCCCCA AATCCCTTTT CCTGACTATA TGGAATTGCA GCAGCGCCTG CTGGCTGGGG 
     3061 GACTCTTTGG TGCTTTGTCC AACAGGGGGA TGGCTTTTCC TTTTCTGGAA GAGTCTAAAG 
     3121 AACTGGGACA CCTGAGTGAA CATGCACTAG TGAGGCAGAA GGAAGAAACT CGCTTTCAGT 
     3181 GTGACATTTG CAAGAAGACC TTTAAAAACG CTTGCAGCAT GAAGACACAC GAGAAGAGTA 
     3241 CACATGCCAG AGAGACACAT GCGTGCACGG TGGACGGCTG CGGGGCTGCT TTCCTTTCCA 
     3301 GGAGGAGCAG AGACAGACAC AGCTCGAACC TAAGCCTCCA CCAGAAAGTA TTGAATGAAG 
     3361 AAGCGTTGGA GACCAGTGAG GACCATTTTC GTGCAGCTTA CCTTCTTCAA GATGTGGCCA 
     3421 AGGAGGCCTA TCAGGATGTG GCTTTCACAC CACAAGCCTC ACAGACATCT GTCATCTTCA 
     3481 AGGGAACAAG CGGCATGGGC AGTCTGGTTT ACCCAATATC CCAAGTTCAC AGTGCCAGCC 
     3541 TGGAGAGCTA CAACTCTGGG CCCCCTAGCG AGGGCACCAT CCTGGATTTG AGCACTACCT 
     3601 CAAGTATGAA GTCAGAGAGC AGCAGTCATT CCTCCTGGGA CTCTGACGGG GTGAGCGAGG 
     3661 AAGGTACCGC CCTTATGGAG GACAGTGATG GCAACTGTGA AGGACAGAGC CTTGTCTCTG 
     3721 CAGAAGATGA GTACCCCATC TGTGTCCTGA TGGAGAAGGC CGACCAGAGC CTTGCTAGTC 
     3781 TGCCCTCTGG TTTGCCAATA ACCTGTCATC TCTGCCAAAA GATATACAGT AACAAAGGGA 
     3841 CCTTCAGAGC CCATTACAAA ACCGTGCACC TCCGCCAGCT CCATAAGTGC AAAGTACCAG 
     3901 GCTGCAACAC GATGTTTTCA TCTGTCCGCA GTAGAAACAG ACACAGTCAG AATCCCAACC 
     3961 TGCACAAGAG CCTGGCTTCA TCTCCAGGCC ACCTCCAGAC GCGTACGCGG CCGCTCGAGC 
     4021 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4081 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4141 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4201 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4261 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4321 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4381 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4441 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4501 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4561 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     4621 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     4681 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     4741 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     4801 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     4861 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     4921 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     4981 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5041 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5101 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5161 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5221 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5281 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5341 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5401 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     5461 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     5521 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     5581 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     5641 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     5701 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     5761 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     5821 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     5881 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     5941 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6001 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6061 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6121 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6181 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6241 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6301 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6361 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6421 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6481 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     6541 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     6601 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     6661 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     6721 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     6781 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     6841 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     6901 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     6961 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7021 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7081 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7141 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7201 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7261 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7321 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7381 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7441 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7501 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     7561 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     7621 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     7681 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     7741 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     7801 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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