LOCUS       RR203407                8379 bp    DNA     circular  SYN 
DEFINITION  Tnks2 (Myc-DDK-tagged ORF) - Rat tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase 2 (Tnks2), (10 ug)
ACCESSION   NM_001107607
VERSION     NM_001107607.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4536..4627 
                     /label="Myc-DDK"  
     misc_feature    4657..5242 
                     /label="PolyA signal"  
     misc_feature    4714..4733 
                     /label="Restriction Enzyme"  
     misc_feature    5518..6190 
                     /label="ColE1"  
     misc_feature    6620..7414 
                     /label="Kan/Neo"  
     misc_feature    7449..7751 
                     /label="SV40 ori"  
     misc_feature    7837..8314 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4518..4524 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4518 
                     /label="Tnks2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTGGCCGC CGCTGCGCCG GCGGGGGCGC GGCCTGCTCG AGCGCGGTGG 
     1081 CGGAGGCCGT AGAGCCGTCC GCCCGCGAAC TGTTCGAGGC TTGCCGCAAC GGGGACGTAG 
     1141 AGCGAGTGAA GAGGCTGGTG ACGCCCGAGA AGGTGAACAG CCGCGACACG GCGGGCAGGA 
     1201 AGTCCACCCC GCTCCACTTC GCCGCAGGTT TTGGTCGGAA GGATGTAGTT GAGTATCTGC 
     1261 TTCAGAATGG TGCAAATGTG CAAGCCCGTG ATGACGGAGG TCTTATTCCT CTTCATAACG 
     1321 CATGCTCTTT TGGTCATGCT GAAGTAGTCA ATCTTCTTTT GCAACATGGT GCAGACCCAA 
     1381 ATGCCCGGGA TAATTGGAAT TATACTCCTC TCCATGAAGC TGCAATTAAA GGAAAAATTG 
     1441 ATGTTTGCAT TGTGCTTTTA CAGCATGGAG CTGAGCCAAC CATCCGAAAT ACAGATGGAA 
     1501 GGACAGCATT GGATTTAGCA GACCCATCCG CCAAAGCTGT TCTGACCGGT GACTATAAGA 
     1561 AAGATGAACT CTTGGAAAGT GCCAGGAGTG GCAATGAGGA GAAAATGATG GCTCTGCTTA 
     1621 CACCACTGAA TGTCAACTGC CATGCAAGTG ATGGCAGAAA GTCAACTCCA TTGCATTTGG 
     1681 CAGCAGGATA CAACAGAGTA AAAATTGTCC AACTGTTACT GCAACATGGA GCAGATGTCC 
     1741 ATGCTAAGGA TAAAGGTGAT CTGGTACCAT TACACAACGC CTGCTCCTAC GGTCATTATG 
     1801 AAGTAACTGA ACTTCTGGTC AAGCATGGTG CCTGTGTAAA TGCAATGGAT TTGTGGCAAT 
     1861 TTACTCCTCT TCATGAGGCA GCTTCTAAGA ACAGGGTTGA AGTGTGTTCT CTTCTCCTAA 
     1921 GTTATGGGGC AGATCCAACC CTGCTGAACT GTCACAATAA GAGTGCTATA GACCTGGCCC 
     1981 CCACAGCACA GCTGAAGGAA AGATTATCGT ATGAGTTTAA AGGCCATTCA TTGCTGCAAG 
     2041 CTGCACGAGA AGCTGATGTT ACAAGAATCA AAAAACATCT TTCTCTGGAA ATGGTGAATT 
     2101 TCAAACATCC TCAAACACAT GAAACAGCAC TGCATTGTGC TGCTGCGTCT CCATATCCTA 
     2161 AAAGAAAGCA AATATGCGAA CTGCTGCTAA GAAAGGGAGC AAACACCAAC GAAAAGACGA 
     2221 AAGAATTCTT GACTCCTCTG CACGTGGCTT CTGAGAATGC TCACAATGAT GTTGTTGAAG 
     2281 TAGTGGTGAA ACACGAAGCA AAGGTTAATG CTTTGGACAG TCTTGGACAG ACATCCTTAC 
     2341 ACAGAGCTGC ACACTGTGGT CATCTGCAGA CTTGCCGCCT TCTCCTGAGC TATGGGTGCG 
     2401 ATCCAAACAT CATCTCCCTT CAGGGTCTCA CTGCCTTGCA AATGGGAAAT GAGAACGTGC 
     2461 AGCAGCTGCT TCAAGAGGGC GTCTCACTAG GTCACTCAGA GGCAGACAGA CAGTTGCTGG 
     2521 AGGCTGCAAA GGCTGGTGAT GTAGAAACTG TAAAGAAACT CTGTACTGTT CAGAGTGTCA 
     2581 ACTGCAGAGA CATTGAAGGA CGGCAGTCAA CCCCACTCCA TTTTGCAGCT GGGTACAACA 
     2641 GAGTGTCTGT GGTAGAGTAT CTCCTGCAGC ACGGAGCTGA CGTACATGCT AAAGACAAAG 
     2701 GAGGTCTTGT TCCTTTGCAC AATGCATGTT CTTACGGACA CTATGAAGTT GCAGAACTTC 
     2761 TTGTCAAGCA TGGAGCAGTA GTTAATGTAG CTGACTTATG GAAGTTCACA CCTCTACATG 
     2821 AAGCTGCAGC AAAAGGAAAA TATGAAATAT GCAAACTTCT GCTCCAGCAT GGTGCAGACC 
     2881 CTACAAAGAA AAATAGAGAT GGTAATACTC CTCTGGATCT CGTTAAAGAT GGAGACACAG 
     2941 ATATTCAAGA TCTACTTAGA GGAGATGCAG CCTTACTAGA TGCTGCCAAG AAGGGTTGTT 
     3001 TAGCCAGAGT GAAGAAATTA TCTTCACCTG ATAATGTAAA TTGCCGTGAT ACCCAAGGTC 
     3061 GACATTCTAC ACCTTTGCAT TTGGCAGCTG GTTATAATAA TTTGGAAGTT GCTGAATATT 
     3121 TGTTGCAACA TGGAGCTGAT GTGAATGCCC AGGACAAAGG AGGACTCATT CCATTACATA 
     3181 ATGCAGCATC ATATGGACAT GTAGACGTAG CAGCTTTACT GATAAAGTAC AATGCGTGTG 
     3241 TCAATGCCAC GGACAAATGG GCTTTCACAC CGTTACATGA AGCAGCCCAA AAGGGACGGA 
     3301 CACAGCTTTG TGCTTTATTG TTGGCCCATG GAGCTGATCC TACTCTTAAA AATCAAGAAG 
     3361 GACAAACACC TTTAGACTTA GTTTCAGCAG ATGATGTCAG CGCTCTCTTG ACAGCAGCCA 
     3421 TGCCCCCCTC TGCTCTGCCT ACGTGCTATA AACCTCAAGT GCTCAGTGGC GTGAGGAGCC 
     3481 CCGGAGCCAC TGCAGACGCC CTGTCTTCAG GTCCGTCCAG TCCATCCAGC CTTTCTGCAG 
     3541 CCAGCAGCCT CGACAACCTC GCTGGCAGTT TTTCTGAACT GTCTGCAGTA GTTAGCTCAA 
     3601 GTACAACGGA AGGTGCTACC GGTTTGGAAA GAAAAGAGGA TTCAGGAATA GATTTTAGTA 
     3661 TAACTCAGTT CATAAGGAAC CTTGGACTTG AGCATTTAAT GGACATATTT GAGCGAGAAC 
     3721 AGATCACCTT GGATGTACTG GTTGAGATGG GCCACAAAGA GCTGAAAGAG ATTGGAATCA 
     3781 GTGCGTATGG ACATCGACAC AGGCTCATTA AAGGGGTCGA AAGACTGATC TCTGGACAAC 
     3841 AAGGTCTTAA TCCATATTTA ACTCTAAACA ACTCTGGTAG TGGAACAATT CTCATAGATC 
     3901 TGTCTCCTGA TGATAAAGAA TTTCAGTCTG TGGAAGAAGA GATGCAAAGT ACTGTTCGAG 
     3961 AGCACAGGGA TGGAGGTCAC GCAGGCGGCA TCTTCAACAG ATACAGTATT CTCAAGATTC 
     4021 AGAAGGTTTG TAACAAGAAA TTGTGGGAAA GATATACACA CCGGAGAAAA GAAGTTTCTG 
     4081 AAGAAAACCA CAACCATGCA AATGAAAGAA TGTTATTTCA TGGGTCTCCC TTTGTGAATG 
     4141 CGATAATCCA TAAGGGCTTT GATGAGAGGC ACGCCTACAT AGGGGGCATG TTTGGAGCTG 
     4201 GGATCTATTT TGCTGAGAAC TCTTCCAAAA GCAATCAGTA TGTGTATGGG ATTGGAGGTG 
     4261 GCACTGGATG TCCAGTTCAC AAAGACAGAT CGTGTTACAT TTGTCACAGG CAGCTGCTCT 
     4321 TTTGTCGCGT GACCTTGGGA AAGTCTTTCC TGCAGTTCAG TGCAATGAAA ATGGCGCACT 
     4381 CTCCTCCCGG CCATCACTCA GTCACTGGCC GGCCCAGTGT GAATGGCCTA GCTTTAGCTG 
     4441 AATATGTTAT TTACAGAGGA GAGCAGGCTT ACCCTGAATA TCTAATTACT TACCAGATTG 
     4501 TAAGGCCTGA AGGTATGGGT GATGGCACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4561 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4621 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4681 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4741 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4801 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4861 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4921 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4981 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     5041 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5101 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5161 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5221 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5281 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5341 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5401 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5461 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5521 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5581 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5641 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5701 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5761 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5821 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5881 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     5941 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     6001 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     6061 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6121 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6181 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6241 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6301 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6361 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6421 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6481 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6541 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6601 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6661 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6721 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6781 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6841 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6901 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6961 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     7021 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     7081 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7141 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7201 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7261 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7321 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7381 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7441 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7501 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7561 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7621 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7681 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7741 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7801 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7861 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7921 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7981 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     8041 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     8101 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8161 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8221 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8281 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8341 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//