LOCUS       RR203360                7989 bp    DNA     circular  SYN 
DEFINITION  Itga4 (Myc-DDK-tagged ORF) - Rat integrin alpha 4 (Itga4), (10 ug)
ACCESSION   NM_001107737
VERSION     NM_001107737.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4146..4237 
                     /label="Myc-DDK"  
     misc_feature    4267..4852 
                     /label="PolyA signal"  
     misc_feature    4324..4343 
                     /label="Restriction Enzyme"  
     misc_feature    5128..5800 
                     /label="ColE1"  
     misc_feature    6230..7024 
                     /label="Kan/Neo"  
     misc_feature    7059..7361 
                     /label="SV40 ori"  
     misc_feature    7447..7924 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4128..4134 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4128 
                     /label="Itga4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTGCGGAA GCGATGTGCA GACCGAGGCC CCGAGGGGTC GCCCTCCAGG 
     1081 AAGCGGTGAT GCTGTTGCTG TACTTCGGGG TACACTTCAG GGTACCAACC GGGCACTCCT 
     1141 ACAACCTGGA CCCGGAGAGT GCACTGCTGT ACCAGGGCCC CTCCGGCACG CTGTTTGGCT 
     1201 ACTCGGTGGT GCTGCACAGC CACGGGACGA AGCGCTGGCT CATTGTGGGG GCGCCCACTG 
     1261 CCAGCTGGCT CGCCAATGCC TCGGTGGTCA ATCCTGGGGC GATTTACAGA TGCAGGATCG 
     1321 GAAAGAATCC AAACCAGATC TGCGAACAGC TCCAGCTGGG AAGCCCCAGT GGAGAACCCT 
     1381 GTGGGAAGAC GTGCTTGGAA GAGAGAGATA ATCAGTGGCT GGGAGTGACA ATTTCCAGAC 
     1441 AGCCAGGAGA AAATGGATCT ATCGTGACTT GTGGGCACAG GTGGAAAAAT ATATTTTACA 
     1501 TGAAGAACAA TAATAAACTC CCCACTGGTG TTTGCTATGG CATGCCTTCT GATTTGCGGA 
     1561 CAGAACTAAG TAAAAGGATG GCCCCCTGTT ACCAAGATTT TGTGAGAAAA TTTGGAGAAA 
     1621 ATTTTGCATC GTGTCAAGCT GGAATATCTA GTTTTTACAC ACAGGATTTG ATTGTGATGG 
     1681 GGGCCCCGGG ATCATCGTAC TGGACCGGCT CCGTCTTTGT CTACAACATA ACTACGAACA 
     1741 AATACAAGGC ATTTGTAGAC AGACAGAATC AAGTAAAATT TGGAAGCTAC TTAGGCTACT 
     1801 CAGTAGGAGC TGGTCATTTC CGAAATCCAC ATACTACCGA AGTCGTGGGA GGAGCCCCTC 
     1861 AACACGAACA GATAGGAAAA GCATATATAT TCAGCATTGA TGAAAGCGAA CTGACCATCG 
     1921 TATATGAAAT GAAAGGTAAA AAGCTTGGCT CTTACTTTGG AGCTTCTGTC TGTGCGGTGG 
     1981 ACCTCAATGC AGATGGCTTC TCAGATCTCC TTGTTGGAGC GCCTATGCAG AGCACCATCA 
     2041 GGGAGGAAGG AAGAGTGTTC GTGTACATCA ACTCTGGCAT GGGAGCTGTG ATGGTTGAAA 
     2101 TGGAAAGAGT GCTCGTCGGA AGTGACAAAT ATGCTGCCAG GTTTGGGGAA TCCATGGCAA 
     2161 ATCTTGGCGA CATTGACAAT GATGGCTTTG AAGATATTGC TATTGGCGCA CCACAAGAAG 
     2221 ATGACTTGCG AGGCGCTGTC TACATTTACA ATGGCCGAGT AGATGGCATC TCCTCTACAT 
     2281 ACTCACAGAG AATCGAAGGA CGTCAAATCA GCCAGTCACT AAGGATGTTT GGACAGTCTA 
     2341 TCTCAGGACA AATTGATGCA GACAACAATG GATATGTTGA TGTAGCGGTT GGTGCATTTC 
     2401 AGTCTGATTC TGCAGTGTTG CTAAGGACAC GGCCTGTAGT GATTGTTGAA GCATCTTTAA 
     2461 GCCATCCTGA GTCAGTAAAT AGAACAAAAT TTGACTGTAC TGAAAATGGA CTTCTGTCTG 
     2521 TGTGCGTGGA TCTTAAACTG TGTTTCTCAT ATAAGGGCAA AGAGGTCCCA GGCTACATCG 
     2581 TTTTGTTTTA CAATGTGAGC TTGGATGTGC ACAGGAAGGC CGAGTCTCCG TCAAGATTTT 
     2641 ATTTCTTCTC CAATGGGACT TCTGACATGA TTACAAGAAG CATACGAGTT TCAAGCAGTG 
     2701 GAGAGAAATG TAGGACACAC CAGGCATTCA TGCGGAAAGA CGTGCGAGAC ATCCTCACTC 
     2761 CCATTCAGGT TGAGGCCACA TACCACCTTG GGCATCATGT GATCACCAAA CGAAACACAG 
     2821 AGGAATTCCC ACCCCTCCAG CCGATCCTTC AGCAGAAGAA AGAAAAAGAC ATTATTAGAA 
     2881 AAACGATAAA CTTCGCAAGG TTTTGTGCCT ATGAAAATTG TTCTGCTGAT CTGCAGGTTT 
     2941 CTGCAAAAGT TGGGTTTTTG AAGCCATATG AAAATAAAAC CTATCTTGCT GTTGGGAGCA 
     3001 TGAAGACCAT AATGCTAAAT GTGTCCTTGT TCAATGCTGG AGATGATGCT TATGAAACTA 
     3061 CTCTGAATGT CCAACTCCCC ACAGGCCTTT ATTTCATTAA GATCTTAGAC CTGGAAGAGA 
     3121 AACAAATAAA CTGCGACGTG ACAGAGAGCT CAGGCGTAGT TAAGCTTGCC TGCAGCCTCG 
     3181 GTTACATATA CGTGGATCGT CTCTCAAGGA TAGACATTAG CTTTCTCCTG GATGTGAGCT 
     3241 CACTCAGCAG GGCCCATGAG GACCTCAGCA TCAACGTGCA TGCCTCCTGT GAAAATGAGG 
     3301 GTAAATTGGA CCAAGTGAAA GACAACAGAG TGACCTTAGT GATACCTCTA AGGTATGAAG 
     3361 TTATGCTGGC TGTTCACGGG TTTGTGACCC CAACTTCGTT TGTGTATGGA TCCAGTGAAG 
     3421 AAAACGAGCC AGAAATGTGC ATGGCAGAGA AATTGAACCT CACTTTCCAT GTTATAAACA 
     3481 CTGGCATTAG CATGGCTCCA AATGTTAGTG TGAAAATAAT GGTACCAAAT TCTTTTCTCC 
     3541 CTCGAAATGA TAAGTTGTTC AACGTTTTGG ATGTCCAGGC AACTATGGGG CAGTGCCAAT 
     3601 TTGAACACTA TGGACGAGAG TGTGCATTAG CACAGCAAAA AGGCATAGCG GGGACCTTGA 
     3661 CCGATTTGGT CAAATTTCTA TCAAAGACTG ATAAGAGACT CCTGTATTGC ATGAAAGCTG 
     3721 ATCGACACTG TTTAGATTTC TTATGTAATT TCGGGAAAAT GGAAAGTGGG AAGGAAGCCA 
     3781 GCGTTCACAT TCAGCTGGAA GGCAGGCCAT CCGTCTTGGA AATGGATGAG ACCTCATCGC 
     3841 TCAAATTTGA AATAAAAGCA ACAGCTTTTC CAGAGCCACA CCCAAAAGTT ATTGAGCTCA 
     3901 ATAAGGATGA GAACATTGCC CATGTTTACT TGGAAGGGCT CCATCATCAA AGACCCAAAC 
     3961 GGCATTTCAC CATCGTGATT GTCACCATCA GCTTGCTCCT TGGACTTATT GTACTTTTAT 
     4021 TAATTTCATG TGTTATGTGG AAGGCTGGAT TTTTTAAAAG GCAGTACAAA TCTATCCTAC 
     4081 AAGAAGAAAA CAGGAGAGAC AGCTGGAGTT ATGTCAACAG CAAAAGCAAT GATGACACGC 
     4141 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4201 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4261 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4321 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4381 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4441 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4501 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4561 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4621 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4681 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     4741 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     4801 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     4861 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     4921 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     4981 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5041 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5101 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5161 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5221 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5281 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5341 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5401 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5461 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     5521 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     5581 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     5641 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     5701 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     5761 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     5821 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     5881 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     5941 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6001 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6061 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6121 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6181 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6241 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6301 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6361 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6421 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6481 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     6541 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     6601 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     6661 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     6721 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     6781 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     6841 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     6901 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     6961 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7021 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7081 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7141 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7201 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7261 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7321 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7381 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7441 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7501 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     7561 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     7621 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     7681 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     7741 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     7801 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     7861 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     7921 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     7981 GCGAATTTT
//