LOCUS       RR203292                8388 bp    DNA     circular  SYN 
DEFINITION  Itgae (Myc-DDK-tagged ORF) - Rat integrin, alpha E, epithelial-associated (Itgae), (10 ug)
ACCESSION   NM_031768
VERSION     NM_031768.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4545..4636 
                     /label="Myc-DDK"  
     misc_feature    4666..5251 
                     /label="PolyA signal"  
     misc_feature    4723..4742 
                     /label="Restriction Enzyme"  
     misc_feature    5527..6199 
                     /label="ColE1"  
     misc_feature    6629..7423 
                     /label="Kan/Neo"  
     misc_feature    7458..7760 
                     /label="SV40 ori"  
     misc_feature    7846..8323 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4527..4533 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4527 
                     /label="Itgae"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGTGGTTC TTCCATGCCC TTCTCTGCGT GGCCAGCCTG AAGCCCTTGG 
     1081 GAGCCTTCAA TGTGGATGTG GACTGGTCCT GGGTCACAGC ACTCCAGCCA GGTGCGCCTT 
     1141 CCGTGCTCAA CTCCCTTCTG CATCAAGACC CCAGCAACAA CCAGACCTGC CTCCTGGTAG 
     1201 CCAGACGCAG CTCCAACAGG AATTCAGCAG TCCTGTATCG ATGTGCTACA TCCATCATTC 
     1261 CAGATGAAAT TGGTTGCCAG CCAGTAGAGC ACATCCTTAT GCCAAAGGGC AGGTACCAGG 
     1321 GAGTGACATT GGTCAGGAAC CACAATGGTG TTTTGGTATG CATTCAGGTA CAGTCCCGGA 
     1381 AGCCCCGCAG CCTTAACTCG GAGCTCACCG GTGCCTGCAG CCTACTTACC CCCAACTTGG 
     1441 ACCTCCAGGC GCAAGCCTAC TTCTCTGACC TTGAAGGAGT CCTGGATCTG GGTGCATCTG 
     1501 TGAACTCTGG TGACTACTAC GGAAGCAAAG GAGGCAGCAC AGGGGAGGAG ACGAAATCAG 
     1561 CCAGGAGACT CCGACGACAG GCAGTGGAGG AGGAAGACGA GGAGGAAGCT GGCACCGAAA 
     1621 TCGCTATTGT TCTGGATGGC TCAGGAAGCA TTGAACCCTC AGACTTCCAG AAGGCCAAAG 
     1681 ATTTCATCTC TACGATGATG AGGAACTTCT ATGAAAAGTG TTTTGAGTGC AACTTTGCCC 
     1741 TGGTGCAGTA TGGGGGCGTG ATCCAGACTG AGTTTGATCT TCTAGACAGC CGGGACATCA 
     1801 ACGCCTCCCT TGCCAAAGTT CAGAGCATCG TTCAAGTGAA GGAAGTCACT AAGACCGCCT 
     1861 CAGCCATGCA GCATGTCCTA GATAATATCT TCATTCCAAG TCGTGGCTCC AGAAAGAAAG 
     1921 CATTGAAGGT TATGGTGGTG CTTACTGATG GTGACATATT TAGGGACCCT CTCAACCTTA 
     1981 CGACTGTCAT CAGTTCCTCA AAGATGCAGG GTGTTGTGCG CTTTGCCATT GGGGTAGGCA 
     2041 ATGCATTCGA GAATAATAAC ACTTACAGAG AACTGAAGCT GATTGCCTCA GACCCTAAAG 
     2101 CGGCCCACAC TTTCAAGGTG ACCAACTACT CGGCTCTGGA TGGGCTGCTG AGCAAGCTAC 
     2161 AGCAGAGGAT CATCCACATG GAAGGCACAG TTGGAGACAC CCTTCAATAT CAACTCGCAC 
     2221 AGACTGGCTT CAGTGCTCAG ATCCTGGACA AGGGGCAGGT GTTGCTTGGC ACTGTGGGGG 
     2281 CCTTTAACTG GTCTGGGGGC GCGCTGCTCT ACAACACACA GAATGGTCGG GGCCGCTTCC 
     2341 TAAACCAGAC AGCCAAGGAG GACTTCAGGG CTGCACAGTA CAGCTACCTC GGTTATTCAG 
     2401 TTGCAGCACT ACACAAGGCC CATGGTGTCT CCTACGTGGC AGGGGCTCCA AGGCACAAGC 
     2461 TCCGTGGGGC TGTGTTTGAG CTCCAGAAGG AGGACGGAGA GGAAACCTTC ATGCGACGGA 
     2521 TAGAAGGAGA GCAGATGGGG TCCTATTTTG GCTCTGTGCT GTGCCCTGTG GATATCAACA 
     2581 TGGATGGAAT CACAGATTTT CTGCTGGTGG CAGCTCCATT TTACCACATC CGTGGAGAAG 
     2641 AAGGCAGAGT CTATGTGTAC CGCGTGCACG AGCAGGATGC TCCCTTCTCC TTGGTATACA 
     2701 CACTGAGTGG GTACCCTGGG CTCACCAGTA GCCGCTTCGG CTTTGCCATG GCAGCTGTGG 
     2761 GGGATATCAA TCAAGATAAA TTCACAGATG TGGCCATTGG GGCCCCTCTG GAGGGCTTTG 
     2821 GGGCAGGCGA TGGGGCAAGC TATGGCAGCG TGTACATCTA CAATGGACAC TCAGGTGGCC 
     2881 TGCATGCCAG TCCCTCCCAG CAGATCAGAG CCTCCTCTGT GGCCTTGGGA CTCTACTACT 
     2941 TTGGCATGTC TGTGTCTGGT GGTTTAGATT TCAGTGGCGA TGACCTTGCC GACATCACAG 
     3001 TTGGCTCTCA GGACGTGGCA GTTGTGCTCC GCTCACGACC TGTGGTGGAC CTGACTGTGT 
     3061 CTATGACCTT TACCCCTGAT GCGCTGCCCA TGGCCTTCAA GGACAAGATG GATGTAGAAC 
     3121 TGTGCTTCAA AGTTGACTCT TCAGCTGTAC CTTCTGAACC AGGCCTTAGA GGGATGTCAC 
     3181 TAAACTTTAC CGTGGATGTG GATGTGACCA AGCAGAAGCA GAGGCTGCAG TGTGCAGACC 
     3241 GGAGTTGTTG TCAAAGCTGC CTTATGAAGT GGAGCGGAGG ATCCTCTCTG TGTGAACACT 
     3301 TCGGGCTCAT CTCCACAGAG GAGGAGCTTT GTGAGGATGA CTGCTTCTCC AACATCACCA 
     3361 TCAAGGTCAG TTATGAGTTC CAGACATCCG AGGAAAGGAG GAACCACCCC AACCCCATCC 
     3421 TGGACCACTA CAAGGAACCC TCTGCCATCT TCCAGCTGCC CTATGAGAAG GACTGCAAGA 
     3481 ACAAAGTGTT CTGCATCGCT GAGATCCAGC TGACCACCGC CATCTCTCAG CAAGACCTGG 
     3541 TGGTGGGTAT CACAAAGGAG GTGACCATGA ACATCAGCCT GACTAACTCT GGGGAAGATT 
     3601 CTTACATGAC AAACATGGCT CTGAATTATC CCAGAAACTT ACAGTTTAAG AAGATACAAA 
     3661 AGCCACTCTC TCCAGATATT CAGTGTGATG ACCCCAAGCC AGTCGCTTCT GTCCTGGTCA 
     3721 TGAACTGCAA GATTGGTCAC CCCATACTCA AGAGATCATC TGTAAATGTC TCAGTGACTT 
     3781 GGCAGCTAGA GGAGAGTATC TTTCCAAATA GGACAGCAGA TATCACTGTG ACCATCTCCA 
     3841 ATTCCAATGA GAAGTCTTTG GCCAGAGAGA CCCACAGCCT TCAATTCAGG CATGCCTTCA 
     3901 TTGCAGTTCT TTCCAGACCG TCAGTGATGT ACATGAACAC GAGCCAAAGC TCTTCTGACC 
     3961 ACAAAGAATT CTTCTTCAAC GTGCACGGGG AGAATCACTT TGGAGCTGTA TTCCAGTTGC 
     4021 AAATTTGTGT CCCCATCACA TTACGAGATT TACAGATTAT AAGAGTGAAG CATCTGACAA 
     4081 AGACTCAGGC CCACACTGAG TGCACCCAGA GTCAAGAGCC CACGTGTGGA AGCGATCCTG 
     4141 TTCAGAATGT GGAAGAATGG CATTCAGTGA TCTGCGCTAT CACATCTAAT AAAGAAAATG 
     4201 TAACCGTGGC CGCAGAGATC TCCATGGGCC ACACTAAGCA GTTACTAAGA GATATAAGTG 
     4261 AACTGCAGAT CCTTGGTGAA ATATCTTTCA ACAAATCTCT CTATGAGGGC CTGAATGCAG 
     4321 AGAACCACAG GACAAAGATC ACAGTCATCT TCCTGAAGGA GGAGAAGCCA CACTCCCTGC 
     4381 CTCTAATCAT TGGCAGCAGT ATCGGAGGCC TCCTGGTCCT GGTCGTGATT ATAGCCATTC 
     4441 TGTTCAAGTG TGGCTTTTTC AAAAGAAAAT ATAAACAGCT GAACTTGGAG AGCGTAAGGA 
     4501 AGGCACAGCT GAAGTCTGAC AGTCTGCTCG AAGATACGCG TACGCGGCCG CTCGAGCAGA 
     4561 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4621 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4681 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4741 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4801 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4861 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4921 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4981 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     5041 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     5101 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5161 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5221 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5281 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     5341 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5401 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5461 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5521 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5581 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5641 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5701 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     5761 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     5821 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5881 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     5941 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     6001 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     6061 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     6121 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     6181 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     6241 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     6301 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6361 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6421 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6481 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6541 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6601 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6661 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6721 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     6781 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6841 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6901 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     6961 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     7021 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     7081 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     7141 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     7201 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     7261 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     7321 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7381 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7441 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7501 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7561 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7621 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7681 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7741 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     7801 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7861 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7921 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     7981 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     8041 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     8101 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     8161 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     8221 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     8281 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     8341 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//