LOCUS       RR203289                8022 bp    DNA     circular  SYN 
DEFINITION  St18 (Myc-DDK-tagged ORF) - Rat suppression of tumorigenicity 18 (St18), (10 ug)
ACCESSION   NM_153310
VERSION     NM_153310.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4177..4268 
                     /label="Myc-DDK"  
     misc_feature    4298..4883 
                     /label="PolyA signal"  
     misc_feature    4355..4374 
                     /label="Restriction Enzyme"  
     misc_feature    5159..5831 
                     /label="ColE1"  
     misc_feature    6261..7055 
                     /label="Kan/Neo"  
     misc_feature    7090..7392 
                     /label="SV40 ori"  
     misc_feature    7478..7955 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4152..4159 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4152 
                     /label="St18"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATGCAGAG GTTGAAGATA AAGCGTTGCA TACTCTCTCA AAAGGAACCG 
     1081 AAGTTCCAAT GGATTCTCTG ATCCCAGAGC TCAGGGTTGC ATATGATTGC TCTATGGCGA 
     1141 AGAAGAGGAG AGCTGAAGAG CAGGCATTGG GAGTGCCAGT TAACAAGAGG AAATCTCTGT 
     1201 TAATGAAACC CCGGCACTAC AGCCCAGACA TGGACTGCAA GGAAAACCCA GACAACAGGA 
     1261 ATGAGGATGA TGGCCTAGAA ACGAATGACC ACTCCACTGC AGATGAAATC GTGGTAAAAC 
     1321 CCATGGATAA GACTCTTCAT TTACCTGCAC AAGAAAGCTC ACTCCCCAAG GAAGACCAAT 
     1381 ACGCGTGTTA TCCAGAGCTC ATGGTCAAAT CTCTGATGCA TTTAGGTAAA TTTGAAGAAA 
     1441 GTGAGTCTGT GCAGACCGTA GGTGAGAACT TAAATGGCAA CGGCATCCAG TCTCTAAAAG 
     1501 CAGAGTGTGA TGAAGCAAAT GAATGCTTTA TGGTTCATTC TGATGATGGA AGAGACAAAG 
     1561 TCCACCATTC CCAGCCACCT TTCTGCTCTT CCGGTGACAG TGAGAGTGAC TCTGACAATA 
     1621 CAGAGAATGG GTGGGGCAGT GGCTCCAACT CTTCAGAAGA CACTGACACT CACAAAGGCC 
     1681 CCAAACGCAA GCTGACATAC AATAGAAAGG ACCTGCTGGA AGTCCCTGAG ATAAAAGCAG 
     1741 AAGATGACAA GTTTATCCCT TGCGAGAACA GGTGTGATTC TGACACCAGT GGGAGAGACC 
     1801 CACAGAACTC TCACATGGAA CCCTTGGCTG TGAAAGTCCA GCCTTCCTTC CCAGAGGTTG 
     1861 AAGAGAGTGA GAGCCTGGCC ACAGTAATAG CGGAGTCTGC TGAGGTGGAG AAAGCGAAGG 
     1921 GGAGCCTGAG TTTGCTGGAA CAGGCTATTG CCCTACAGGC TGAACGGGGT TCCGTCTTCC 
     1981 ATCACACCTA CAAAGAGCTG GACCGATTTC TCCTGGACCA CCTGGCAAGG CAACGGAGGC 
     2041 AACCCAAGGT TACTGATGCC AGTGGAAGGC AAATCTTTAA TAATAAACAT TCACCAAGGC 
     2101 CTGAAAGGAG GGAGGCTAAG TGCCCCATTC CTGGCTGTGA CGGCACAGGG CACGTAACGG 
     2161 GGCTCTACCC CCACCACCGC AGCCTTTCTG GGTGCCCCCA CAAAGTGCGA GTTCCTCTGG 
     2221 AAATTCTTGC CATGCATGAG AACGTACTCA AGTGTCCCAC CCCAGGATGC ACAGGAAGGG 
     2281 GACATGTGAA CAGCAATCGC AACACTCACA GAAGTCTTTC TGGTTGTCCA ATTGCTGCAG 
     2341 CTGAAAAGTT GGCAATGACC CAGGACAAAA GTCAACTTGA TTCTTCCCAG ACTGGGCAGG 
     2401 GTCCTGAGCA GGCTCACAGG GTGAATTTAG TGAAGCAAAT TGAATTTAAC TTCCGCTCAC 
     2461 AAGCCATCAC CTCTCCAAGA GCCTCTGCAT CAAAAGAACA AGAGAAGTTT GGCAAAGTAC 
     2521 CATTTGATTA TGCCAGTTTT GATGCACAAG TATTTGGTAA ACGTCCGCTC CTACAAACAG 
     2581 GACAAGGACA AAAGGCACCA CCATTTCCTG AATCAAAGCA TTTTTCAAAT CCAGTGAAAT 
     2641 TTTCTAATGG GCTGCCTAGT GCTGGCGCCC ACACACAGAG CACAGTCCGT GCCAGCTCTT 
     2701 ATGGCCATGG TCAGTACAGT GAAGACACCC ACATAGCAGC AGCTGCTGCT ATCCTGAACC 
     2761 TTTCCACCCG CTGCAGGGAA GCCACAGACA TCCTCTCCAA CAAACCGCAA AGCCTGCGTG 
     2821 CCAAGGGAGC TGAGATAGAA GTGGATGAAA ACGGCACGTT GGACTTAAGC ATGAAAAAAC 
     2881 ATCGAATCCT CGACAAGTCT ATACCCCCGA CTTCCTCACA CACTACAATT GCAACCCCAT 
     2941 CCTCATCCCC ATTCAAAGCA AGCAGCCTTC TGGTCAATGC TGCCTTCTAT CAGGCTCTCT 
     3001 GTGACCAAGA AGGCTGGAAT GTGCCTATCA ACTATAGCAA ATCCCACGGG AAGACAGAGG 
     3061 AGGAGAAAGA GAAAGACCCT GTGAACTCCC TAGAAAATTT AGAGGAAAAA AAGTTTGCTG 
     3121 GAGAGGCCTC TATTCCAAGT CCAAAGCCCA AGCTCCACAC ACGAGATCTC AAGAAAGAAT 
     3181 TAATCACCTG CCCCACGCCA GGGTGTGATG GAAGCGGCCA TGTCACAGGC AACTATGCAT 
     3241 CTCACCGCAG TGTCTCTGGA TGTCCTCTAG CAGATAAGAC TCTGAAGTCC CTCATGGCTG 
     3301 CCAACTCTCA AGAGCTTAAG TGTCCAACCC CGGGTTGTGA TGGTTCTGGT CACGTGACTG 
     3361 GAAACTATGC CTCCCATAGA AGCTTGTCTG GTTGCCCTCG TGCAAGGAAA GGTGGCATCA 
     3421 AAATGACCCC AACAAAGGAA GAAAAAGAAG ACTCTGAGCT TAGATGTCCT GTAATAGGAT 
     3481 GTGACGGCCA AGGTCACATA TCAGGTAAAT ACACATCACA CCGCACAGCT TCTGGCTGTC 
     3541 CTCTGGCTGC CAAGAGACAG AAGGAGAATC CCCTCAATGG GACCCCTCTC TCATGGAAAC 
     3601 TGAACAAGCA AGAACTTCCC CACTGTCCTT TGCCAGGATG CAATGGTCTG GGTCATGTAA 
     3661 ACAACGTTTT TGTCACCCAC AGGAGCTTAT CCGGATGCCC TCTTAATGCA CAAGCTATCA 
     3721 AAAAAGTCAA GGTCTCTGAG GAACTAATGA CCATCAAGCT CAAAGCAACT GGGGGTATTG 
     3781 AGGGTGATGA AGAAATTAGG CATCTGGATG AAGAAATAAA GGAACTGAAT GAATCCAACC 
     3841 TTAAAATTGA AGCAGATATG ATGAAACTTC AGACCCAGAT AACATCTATG GAGAGCAACT 
     3901 TGAAGACCAT AGAGGAGGAG AACAAGCTTA TAGAGCAGAG CAACGAGAGT CTACTGAAGG 
     3961 AGCTGGCAGG GCTTAGCCAG GCTCTCATCT CCAGTCTTGC TGACATCCAG CTTCCACAGA 
     4021 TGGGTCCTAT CAATGAGCAG AATTTTGAAG CATATGTAAA TACTCTCACA GACATGTACA 
     4081 GCAATCTGGA ACGGGACTAT TCCCCAGAAT GCAAAGCTCT ACTGGAAAGC ATCAAGCAGG 
     4141 CAGTGAAAGG TATCCATGTG AGCGGACCGA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4201 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4261 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4321 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4381 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4441 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4501 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4561 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4621 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4681 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     4741 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     4801 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     4861 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     4921 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     4981 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5041 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5101 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5161 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5221 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5281 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5341 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5401 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5461 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5521 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5581 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     5641 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     5701 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     5761 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     5821 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     5881 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     5941 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6001 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6061 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6121 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6181 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6241 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6301 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6361 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6421 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6481 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6541 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6601 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     6661 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     6721 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     6781 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     6841 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     6901 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     6961 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7021 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7081 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7141 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7201 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7261 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7321 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7381 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7441 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7501 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7561 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7621 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     7681 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     7741 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     7801 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     7861 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     7921 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     7981 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//