LOCUS       RR203270                8604 bp    DNA     circular  SYN 
DEFINITION  Sbno1 (Myc-DDK-tagged ORF) - Rat strawberry notch homolog 1 (Drosophila) (Sbno1), (10 ug)
ACCESSION   NM_001107138
VERSION     NM_001107138.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4761..4852 
                     /label="Myc-DDK"  
     misc_feature    4882..5467 
                     /label="PolyA signal"  
     misc_feature    4939..4958 
                     /label="Restriction Enzyme"  
     misc_feature    5743..6415 
                     /label="ColE1"  
     misc_feature    6845..7639 
                     /label="Kan/Neo"  
     misc_feature    7674..7976 
                     /label="SV40 ori"  
     misc_feature    8062..8539 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4743..4749 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4743 
                     /label="Sbno1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGGAACCA GGGCAAGATT TACTACTTGC GGCCTTGAGC GAGAGTGGGA 
     1081 TTAGTCCGAA TGACCTCTTT GATGTTGATG GTGGAGATGC TGGACTTGCC ACCCCTACTC 
     1141 CCCCTTCAGT TCAACAGTCG GTGCCACTTA GTGCATTAGA ACTAGGTTTG GAGACAGAAG 
     1201 CAGCAATTCC TGTTAAACAA GAACCCGAGA TGATGTCTAC TCCGGCACTG TTAAATGTGA 
     1261 GGCAGCAGCC TCCATCCACT ACAACATTTG TGCTGAATCA AATCAATCAA CTTCCAACCT 
     1321 TGGGATCTAC AATTGTAATG ACTAAAACAC CACCTGCAAC AACGAATAGG CAAACCATCA 
     1381 CTTTGACTAA GTTCATCCAG ACCACTGCAA ACACACGCCC TTCAGTTTCA GCACCAGCAG 
     1441 TACGGAATGC CATCCCCCCT GCTCCCGCAA AAGACCAAGT TCAGCTAAAG GATTTACTCA 
     1501 AAAATAATAG TTTAAATGAA CTCATGAAAC TGAAGCCACC TGCCAATATT GCTCAGCCAG 
     1561 TTGCAACTGC GGCTACTGAT GTCAGCAATG GCGCAGTTAA AAAGGAGTCT TCTAATAAAG 
     1621 AAGTTGCAAG GGTATGGATA AATGACATGA AGATGAGAAG CTTTTCCCCC ACTATGAAGG 
     1681 TTCCTGTTGT GAGAGAAGAA GATGAACCAG AAGAAGAAGA TGAGGAAGAA ATGGGTCATG 
     1741 CAGAAACCTA TGCAGAGTAT ATGCCAATAA AATTAAAAAT TGGCCTGCGT CACCCTGATG 
     1801 CGGTGGTGGA GACAAGCTCA CTGTCCAGCG TCACTCCTCC TGATGTTTGG TACAAAACGT 
     1861 CCATCTCTGA GGAGACCATC GATAATGGCT GGCTCTCTGC ATTGCAGCTT GAGGCAGTCA 
     1921 CCTATGCGGC CCAGCAACAT GAAACATTCC TTCCTAATGG AGACCGTGCT GGCTTCTTAA 
     1981 TAGGCGATGG TGCTGGTGTG GGCAAAGGCC GGACGATAGC AGGGATCATA TATGAAAACT 
     2041 ATTTGTTGAG TAGAAAAAGA GCATTGTGGT TTAGTGTATC AAATGACTTA AAATATGATG 
     2101 CTGAAAGAGA TTTAAGGGAT ATTGGAGCAA AAAACATTTT GGTCCATTCA TTAAATAAGT 
     2161 TTAAATATGG AAAGATTTCT TCTAAACACA ATGGGAGTGT GAAAAAGGGT GTTATTTTTG 
     2221 CTACCTATTC TTCCCTTATT GGTGAAAGCC AGTCTGGAGG TAAATATAAG ACCAGGTTAA 
     2281 AACAGCTTCT GCACTGGTGT GGTGACGACT TCGATGGAGT GATAGTGTTT GATGAATGTC 
     2341 ACAAAGCAAA AAACTTATGT CCTGTTGGTT CTTCAAAGCC AACCAAGACA GGCTTGGCTG 
     2401 TTTTAGAACT TCAAAACAAA TTGCCAAAAG CCAGAGTCGT GTATGCCAGT GCCACCGGTG 
     2461 CTTCTGAACC ACGCAACATG GCCTATATGA ACCGTCTTGG AATATGGGGT GAGGGAACTC 
     2521 CATTCAGAGA ATTCAGTGAT TTTATCCAAG CTGTGGAACG CAGAGGTGTT GGTGCCATGG 
     2581 AAATTGTTGC TATGGATATG AAGCTTAGAG GAATGTACAT TGCACGACAG CTGAGCTTTA 
     2641 CTGGAGTGAC CTTCAAAATT GAGGAAGTTC TTCTTTCTCA AAGCTACGTT AAAATGTATA 
     2701 ACAAAGCAGT CAAACTGTGG GTTATCGCTA GAGAGCGATT TCAGCAAGCT GCAGATTTGA 
     2761 TTGATGCTGA GCAGAGAATG AAGAAATCTA TGTGGGGTCA GTTCTGGTCT GCTCACCAGA 
     2821 GGTTTTTTAA GTACCTGTGT ATAGCGTCCA AAGTGAAGAG GGTGGTCCAG CTAGCCCGGG 
     2881 AAGAAATCAA GAATGGGAAG TGTGTTGTAA TTGGTCTGCA GTCCACAGGA GAAGCCAGAA 
     2941 CTTTAGAGGC CTTGGAAGAG GGTGGAGGAG AACTAAATGA TTTTGTTTCA ACTGCCAAAG 
     3001 GGGTATTGCA GTCACTGATT GAAAAACACT TCCCTGCTCC AGACAGGAAG AAACTCTATA 
     3061 GTTTGCTAGG AATCGACTTG ACAGCCCCAA GTAACAACAG TTCACCAAGA GATAGTCCTT 
     3121 GTAAAGAGAA TAAAATAAAG AAGCGGAAAG GTGAAGAAAT TACTCGAGAA GCCAAAAAAG 
     3181 CACGAAAAAT TGGTGGCCTT ACTGGAAGCA GCTCTGACGA CAGTGGGAGT GAATCTGATG 
     3241 TCTCTGATAA TGAAGAAAGC GACTATGAGA GCTCTAAAAA CATGAGCTCT GGTGATGACG 
     3301 ATGATTTCAA TCCCTTCAGA GATGAGTCCA GCGAAGACAA CGAGGACGAT CCATGGTTAA 
     3361 TTAGGAAAGA TCATAAGAAA AGCAAAGATA AAAAAAAGAA GAAAAGTATC GATCCAGATT 
     3421 CCATTCAAAG TGCCTTGTTA GCATCTGGTC TTGGATCAAA GCGGCCCAGT TTCTCCTCAG 
     3481 CACCAGTCAT CTCACCTGCT TCCAACAGTG CACCAGCCAA CAGTAACAGC AACAGTAACA 
     3541 GCAGCCTCAT AACGAGCCAA GATGCTGTTG AAAGGGCACA GCAGATGAAG AAAGACCTCC 
     3601 TTGATAAGCT GGAAAAGTTA GCTGAGGACC TCCCGCCTAA TACCCTGGAT GAGCTTATCG 
     3661 ATGAGCTCGG TGGTCCTGAA AATGTGGCTG AGATGACTGG CCGCAAGGGG CGGGTGGTGA 
     3721 GCAATGATGA TGGAAGCATA TCCTACGAGT CAAGGTCTGA ACTTGATGTG CCTGTGGAGA 
     3781 TTCTGAACAT CACGGAGAAG CAGAGGTTCA TGGATGGAGA CAAGAATATT GCTATTATCT 
     3841 CAGAAGCTGC CAGCTCAGGT ATTTCATTAC AAGCAGATCG GAGAGCTAAG AATCAAAGGA 
     3901 GAAGAGTTCA TATGACTTTA GAGTTACCCT GGAGTGCTGA TCGGGCCATT CAGCAGTTCG 
     3961 GAAGAACACA CAGGTCAAAC CAAGTGACTG CTCCAGAGTA TGTTTTTCTG ATTTCGGAGC 
     4021 TGGCTGGAGA ACAGAGGTTT GCGTCTATTG TTGCTAAGAG ACTTGAGAGT CTGGGGGCAC 
     4081 TTACACATGG CGACAGAAGA GCAACAGAAT CTAGAGATCT CAGCAGGTTC AACTTTGACA 
     4141 ACAAGTATGG AAGAAATGCT TTAGAAATTG TTATGAAGTC CATTGTAAAC TTGGATTCTC 
     4201 CTATGGTATC ACCACCTCCA GACTACCCTG GAGAATTCTT TAAAGATGTT CGGCAAGGAC 
     4261 TGATAGGAGT GGGCTTGATT AATGTAGAAG ATCGCTCAGG AATTCTTACT CTTGATAAAG 
     4321 ACTATAATAA TATAGGAAAA TTCTTAAACA GAATTCTGGG CATGGAGGTG CATCAGCAGA 
     4381 ATGCGTTGTT TCAGTATTTT GCAGACACAC TCACTGCAGT TGTTCAGAAT GCCAAGAAGA 
     4441 ATGGGAGATA CGACATGGGA ATCCTAGATC TGGGTTCTGG AGATGAAAAG GTGAGGAAGA 
     4501 GTGATGTCAA GAAGTTTCTG ACTCCAGGAT ACTCAACCTC TGGCCATGTA GAGTTATATA 
     4561 CAATTAGTGT GGAGAGGGGA ATGTCCTGGG ATGAAGCAAC CAAGATTTGG GCCGAGCTGA 
     4621 CAGGACCCGA CGATGGCTTT TACTTATCGT TGCAATGTTC GTTTTGGAGG AGAATAGGCC 
     4681 TTAGGAAGGC CTATGTTAAC TTGACTGCTG TTGACTGTGC CGCTGGCGTG GCAATTGCAA 
     4741 AAAAGCAAGC TACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4801 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4861 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4921 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4981 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     5041 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     5101 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     5161 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5221 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5281 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5341 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5401 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5461 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5521 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5581 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5641 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5701 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5761 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5821 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5881 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5941 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     6001 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     6061 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     6121 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6181 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6241 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6301 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6361 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6421 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6481 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6541 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6601 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6661 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6721 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6781 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6841 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6901 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6961 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     7021 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     7081 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     7141 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7201 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7261 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7321 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7381 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7441 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7501 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7561 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7621 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7681 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7741 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7801 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7861 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7921 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7981 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     8041 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     8101 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     8161 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8221 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8281 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8341 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8401 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8461 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8521 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8581 TAACAAAAAT TTAACGCGAA TTTT
//