LOCUS       RR203228                7548 bp    DNA     circular  SYN 
DEFINITION  Aco1 (Myc-DDK-tagged ORF) - Rat aconitase 1, soluble (Aco1), (10 ug)
ACCESSION   NM_017321
VERSION     NM_017321.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3705..3796 
                     /label="Myc-DDK"  
     misc_feature    3826..4411 
                     /label="PolyA signal"  
     misc_feature    3883..3902 
                     /label="Restriction Enzyme"  
     misc_feature    4687..5359 
                     /label="ColE1"  
     misc_feature    5789..6583 
                     /label="Kan/Neo"  
     misc_feature    6618..6920 
                     /label="SV40 ori"  
     misc_feature    7006..7483 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3687..3693 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3687 
                     /label="Aco1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGAATCCA TTTGCGCACC TTGCCGAGCC CTTGGACCCT GCACAGCCAG 
     1081 GAAAGAAATT CTTCAACTTG AATAAATTGG AGGACTCAAG ATATGGACGC TTACCGTTCT 
     1141 CTATCAGAGT TCTCCTGGAG GCCGCCGTTC GGAACTGTGA CGAGTTTTTG GTGAAGAAGA 
     1201 ATGACATTGA GAATATCCTG AACTGGAGTA TCATGCAGCA TAAGAGCATA GAAGTGCCGT 
     1261 TTAAGCCAGC CCGAGTCATA CTGCAGGACT TTACGGGCGT GCCTGCTGTG GTTGATTTTG 
     1321 CAGCAATGCG CGATGCTGTG AAGAAGTTGG GAGGAAATCC AGAGAAAATA AACCCTGTCT 
     1381 GCCCCGCTGA CCTTGTAATC GATCATTCCA TCCAGGTTCA TTTCAACAGA AGGGCAGACA 
     1441 GTTTGCAGAA GAATCAAGAC CTGGAATTTG AAAGGAATAG AGAACGATTT GAATTTTTAA 
     1501 AGTGGGGTTC CCAGGCCTTT TGCAACATGA GGATTATTCC TCCTGGCTCA GGAATTATTC 
     1561 ACCAAGTGAA TCTGGAGTAT TTGGCAAGAG TAGTGTTTGA TCAGGATGGA TGTTACTACC 
     1621 CAGATAGCCT CGTGGGCACA GATTCTCACA CCACCATGAT TGATGGTCTG GGAGTTCTTG 
     1681 GTTGGGGTGT AGGTGGTATT GAAGCAGAAG CTGTCATGCT GGGTCAGCCA ATCAGCATGG 
     1741 TGCTTCCCCA GGTGATTGGC TACAAGCTGA TGGGGAAGCC TCACCCTCTG GTAACCTCCA 
     1801 CGGACATTGT GCTCACCATT ACCAAGCACC TCCGACAAGT TGGGGTCGTG GGCAAATTCG 
     1861 TGGAGTTTTT CGGGCCAGGA GTGGCCCAGC TGTCCATTGC TGACCGAGCT ACGATTGCCA 
     1921 ACATGTGCCC AGAGTACGGC GCGACGGCAG CCTTCTTCCC GGTCGACGAC GTTAGCATCG 
     1981 CGTACCTGGT GCAGACAGGT CGTGAGGAAG ACAAAGTAAA GCACATTAAA AGGTATCTTC 
     2041 AGGCTGTAGG CATGTTTCGA GACTTCAGTG ACTCCTCTCA AGACCCAGAC TTCACTCAGG 
     2101 TCGTGGAGTT AGATTTGAAA ACAGTTGTGC CTTGCTGCAG TGGACCTAAA AGACCTCAGG 
     2161 ACAAAGTTGC TGTGTCTGAG ATTGAAAAGG ACTTTGAAAG CTGCCTTGGA GCCAAGCAAG 
     2221 GATTTAAAGG TTTTCAAGTT GCTCCAGACC ATCACAATGA CCACAAGACG TTTATCTATA 
     2281 ACGACAGTGA ATTCACTCTT GCTCATGGCT CCGTGGTGAT CGCCGCCATC ACTAGCTGCA 
     2341 CAAACACCAG CAATCCGTCC GTGATGTTAG GCGCAGGATT GTTAGCAAAG AAAGCCGTAG 
     2401 AGGCTGGCCT GAATGTGAAG CCTTACGTCA AAACCAGCCT GTCTCCTGGG AGTGGAGTGG 
     2461 TCACCTACTA CCTTCGAGAG AGTGGAGTCA TGCCTTACCT GTCCCAGTTA GGGTTTGACG 
     2521 TGGTGGGCTA CGGCTGCATG ACCTGCATCG GCAACAGTGG ACCCCTCCCC GAACCTGTGG 
     2581 TGGAGGCTAT CACCCAGGGA GACCTTGTGG CTGTTGGGGT ACTGTCTGGA AACAGGAATT 
     2641 TTGAAGGACG AGTCCATCCT AACACCCGGG CCAACTACTT AGCATCTCCC CCACTAGTAA 
     2701 TAGCATATGC AATTGCAGGC ACCGTCAGGA TCGACTTCGA GAAAGAGCCT TTGGGAGTGA 
     2761 ACGCACAGGG CCAACAAGTG TTTCTGAAGG ATATCTGGCC AACTCGAGAT GAGATCCAGG 
     2821 AGGTGGAGCG GAAGTATGTC ATCCCCGGCA TGTTCAAGGA GGTCTATCAG AAGATAGAGA 
     2881 CTGTAAACAA AAGCTGGAAT GCCTTAGCAG CCCCTTCAGA GAAGCTGTAT GCGTGGAACC 
     2941 CCAAGTCTAC TTATATCAAG TCACCGCCAT TCTTTGAAAG CTTGACTTTA GATCTCCAGC 
     3001 CCCCCAAGTC TATAGTGGAT GCCTATGTGC TACTAAATCT AGGAGATTCC GTAACAACGG 
     3061 ACCATATCTC TCCAGCGGGG AACATTGCAA GAAACAGCCC TGCCGCTCGC TACTTGACGA 
     3121 ACAGAGGCCT GACGCCACGA GATTTCAACT CCTACGGCTC CCGCCGGGGT AACGACGCCA 
     3181 TCATGGCACG GGGGACATTT GCCAACATTC GCTTGCTGAA CAAGTTTCTG AACAAGCAGG 
     3241 CCCCTCAGAC TGTCCACCTT CCTTCAGGAG AAACCCTCGA TGTGTTCGAT GCCGCTGAGC 
     3301 GGTACCAGCA GGCTGGACTT CCCCTGATTG TTCTGGCTGG CAAAGAGTAC GGTTCAGGCA 
     3361 GCTCCCGAGA CTGGGCAGCC AAAGGTCCTT TCCTGCTGGG AATCAAAGCT GTCCTGGCAG 
     3421 AGAGCTACGA GCGCACTCAC TGCAGCAACC TGGTTGGCAT GGGGGTGATC CCCCTTGAGT 
     3481 ATCTCCCCGG CGAAACTGCA GACTCTCTGG GACTCACGGG TCGGGAAAGG TACACGATCC 
     3541 ACATTCCCGA ACACCTTAAG CCCCGCATGA AGGTTCAGAT AAAGCTGGAC ACCGGGAAGA 
     3601 CCTTCCAGGC CGTGATGAGG TTCGACACCG ACGTGGAGCT CACTTACTTC CACAATGGAG 
     3661 GCATCCTGAA CTACATGATC CGAAAGATGG CCCAGACGCG TACGCGGCCG CTCGAGCAGA 
     3721 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     3781 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     3841 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     3901 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     3961 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4021 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4081 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4141 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4201 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4261 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     4321 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4381 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4441 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     4501 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     4561 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     4621 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     4681 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     4741 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     4801 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     4861 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     4921 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     4981 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5041 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     5101 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     5161 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     5221 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     5281 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     5341 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     5401 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     5461 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     5521 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     5581 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     5641 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     5701 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     5761 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     5821 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     5881 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     5941 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6001 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6061 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     6121 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     6181 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     6241 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     6301 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     6361 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     6421 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     6481 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     6541 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     6601 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     6661 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     6721 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     6781 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6841 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     6901 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     6961 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7021 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7081 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     7141 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     7201 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     7261 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     7321 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     7381 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     7441 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     7501 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//