LOCUS       RR203199                8403 bp    DNA     circular  SYN 
DEFINITION  Polr2b (Myc-DDK-tagged ORF) - Rat polymerase (RNA) II (DNA directed) polypeptide B, 140kDa (Polr2b), (10 ug)
ACCESSION   NM_001106002
VERSION     NM_001106002.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4560..4651 
                     /label="Myc-DDK"  
     misc_feature    4681..5266 
                     /label="PolyA signal"  
     misc_feature    4738..4757 
                     /label="Restriction Enzyme"  
     misc_feature    5542..6214 
                     /label="ColE1"  
     misc_feature    6644..7438 
                     /label="Kan/Neo"  
     misc_feature    7473..7775 
                     /label="SV40 ori"  
     misc_feature    7861..8338 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4542..4548 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4542 
                     /label="Polr2b"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTATGACGCC GACGAGGATA TGCAATATGA TGAAGATGAT GATGAAATCA 
     1081 CTCCAGATTT GTGGCAGGAA GCATGCTGGA TCGTGATCAG TTCTTATTTT GATGAAAAAG 
     1141 GCTTGGTCAG ACAGCAGCTG GATTCCTTTG ACGAGTTTAT TCAGATGTCT GTTCAAAGAA 
     1201 TTGTGGAAGA TGCACCACCG ATTGACCTGC AGGCTGAAGC CCAGCACGCT AGTGGGGAGG 
     1261 TTGAAGAGCC GCCAAGATAT TTACTGAAGT TTGAACAAAT TTACCTCTCC AAACCTACTC 
     1321 ATTGGGAAAG AGATGGTGCC CCTTCACCAA TGATGCCAAA TGAAGCCAGG TTAAGAAATC 
     1381 TCACGTACTC CGCCCCGCTT TACGTGGACA TAACGAAGAC GGTCATTAAA GAGGGTGAAG 
     1441 AGCAGCTGCA GACTCAGCAC CAGAAAACCT TCATCGGGAA AATCCCCATT ATGCTGCGCT 
     1501 CCACGTATTG CTTGTTGAAT GGCTTAACAG ACCGTGACTT GTGTGAGCTG AATGAGTGTC 
     1561 CTTTGGATCC TGGTGGCTAT TTTATTATTA ATGGATCAGA AAAGGTTCTG ATAGCTCAAG 
     1621 AGAAAATGGC AACAAACACA GTCTATGTGT TTGCCAAAAA GGATTCCAAG TATGCCTATA 
     1681 CAGGAGAATG CCGCTCATGC CTAGAGAATT CTTCCCGACC CACCAGTACT ATATGGGTTA 
     1741 GCATGCTGGC CAGAGGAGGA CAGGGTGCAA AGAAGAGTGC AATTGGCCAG CGCATCGTGG 
     1801 CGACATTACC ATATATCAAG CAAGAAGTTC CCATCATAAT TGTGTTCAGA GCATTAGGCT 
     1861 TTGTGTCTGA TAGAGATATT TTAGAGCACA TCATTTATGA CTTCGAAGAC CCAGAGATGA 
     1921 TGGAAATGGT TAAACCATCT CTCGATGAAG CATTTGTCAT CCAAGAGCAG AATGTTGCCT 
     1981 TAAACTTCAT TGGTTCAAGA GGGGCGAAGC CTGGTGTTAC TAAGGAGAAA AGAATTAAAT 
     2041 ATGCAAAAGA AGTCTTACAG AAAGAGATGC TTCCGCATGT CGGTGTCAGT GATTTCTGTG 
     2101 AGACCAAAAA GGCCTATTTC TTGGGGTACA TGGTTCATAG GTTACTTCTA GCAGCCTTGG 
     2161 GTAGAAGAGA ACTAGATGAC AGAGATCACT ATGGCAACAA GAGATTGGAT CTTGCTGGGC 
     2221 CTCTGCTTGC GTTCTTATTT AGAGGCATGT TTAAGAATTT GCTGAAAGAA GTACGGATCT 
     2281 ATGCACAGAA GTTTATTGAC CGCGGAAAGG ACTTTAACCT AGAGTTGGCA ATTAAGACAA 
     2341 GGATCATATC CGATGGGCTA AAATACTCTT TAGCCACTGG GAACTGGGGT GACCAGAAGA 
     2401 AAGCTCACCA AGCCAGAGCT GGAGTGTCGC AGGTACTGAA CCGCCTGACT TTTGCGTCTA 
     2461 CTCTTTCTCA CCTGCGTCGT TTAAACTCTC CTATTGGCAG AGATGGCAAG CTAGCAAAGC 
     2521 CGAGACAGTT GCATAATACA TTGTGGGGGA TGGTGTGTCC TGCTGAAACT CCAGAGGGCC 
     2581 ATGCTGTAGG ACTTGTGAAG AATTTAGCCC TGATGGCTTA TATTTCAGTT GGATCTCAAC 
     2641 CTTCTCCAAT TTTGGAATTT TTGGAAGAAT GGAGCATGGA AAACTTAGAG GAAATTTCCC 
     2701 CTGCAGCTAT TGCCGATGCA ACCAAGATTT TTGTTAATGG CTGCTGGGTT GGAATCCATA 
     2761 AAGACCCGGA ACAACTCATG AACACACTCA GGAAATTACG GCGTCAGATG GACATCATTG 
     2821 TATCCGAAGT TTCTATGATC AGAGATATCC GCGAGCGTGA GATTCGGATC TATACAGACG 
     2881 CAGGCCGTAT TTGTAGACCA CTTCTAATTG TGGAAAAGCA AAAGCTTCTT TTGAAGAAGA 
     2941 GACACATTGA CCAGTTGAAA GAGAGAGAAT ATAACAACTA CAGTTGGCAG GATCTGGTAG 
     3001 CCAGTGGGGT AGTAGAGTAT ATTGATACCC TGGAAGAAGA AACCGTGATG CTTGCAATGA 
     3061 CTCCAGATGA TTTGCAGGAG AAAGAAGTAG CTTATTGCTC CACCTATACC CACTGTGAGA 
     3121 TTCACCCATC AATGATTCTT GGCGTCTGTG CATCTATTAT TCCTTTTCCT GATCATAACC 
     3181 AGTCCCCTAG AAACACGTAC CAGTCTGCTA TGGGCAAGCA GGCTATGGGA GTTTATATCA 
     3241 CCAACTTCCA CGTTCGGATG GACACCTTGG CGCACGTTCT CTACTATCCT CAGAAGCCCC 
     3301 TTGTGACTAC ACGGTCTATG GAGTATCTCC GGTTTAGAGA ACTGCCAGCA GGAATCAACT 
     3361 CAATTGTGGC CATTGCATCA TACACGGGCT ATAATCAAGA AGACTCTGTT ATCATGAATC 
     3421 GTTCAGCTGT GGATCGAGGC TTTTTCAGGT CTGTTTTCTA CCGATCATAC AAAGAACAAG 
     3481 AGTCTAAAAA AGGATTTGAT CAAGAAGAAG TGTTCGAGAA GCCTACACGT GAAACATGTC 
     3541 AGGGCATGAG GCATGCCATT TATGACAAGT TGGACGATGA TGGCTTGATA GCGCCAGGGG 
     3601 TCCGAGTGTC GGGAGACGAT GTTATTATAG GCAAAACGGT CACCCTCCCT GAGAACGAGG 
     3661 ATGAGCTGGA GAGCACCAAC AGGCGCTACA CCAAGAGGGA CTGCAGCACC TTCCTCAGAA 
     3721 CCAGTGAGAC AGGCATTGTG GACCAGGTCA TGGTCACTCT CAACCAGGAA GGATATAAAT 
     3781 TTTGTAAAAT AAGGGTACGC TCTGTTAGAA TTCCGCAGAT CGGAGACAAA TTTGCTAGCC 
     3841 GACATGGTCA GAAGGGTACT TGTGGTATTC AGTATAGACA AGAGGACATG CCGTTCACTT 
     3901 GTGAAGGCAT AACTCCTGAC ATCATCATAA ACCCCCACGC CATCCCCTCT CGGATGACGA 
     3961 TTGGTCACTT GATTGAATGT CTCCAAGGGA AGGTCTCTGC GAACAAGGGT GAAATTGGTG 
     4021 ATGCTACTCC GTTTAATGAT GCTGTCAATG TGCAGAAGAT TTCTAATCTT TTATCTGACT 
     4081 ACGGCTACCA TCTCAGAGGA AATGAGGTGC TCTACAATGG ATTCACTGGT CGCAAAATCA 
     4141 CATCGCAGAT TTTTATTGGC CCCACTTACT ACCAGCGTTT GAAGCATATG GTGGATGACA 
     4201 AAATTCATTC TCGTGCTCGG GGGCCCATTC AGATTCTTAA CAGACAGCCC ATGGAGGGCA 
     4261 GATCGCGTGA TGGTGGCCTG CGCTTTGGAG AAATGGAGCG AGACTGTCAG ATCGCACATG 
     4321 GTGCAGCACA GTTCTTAAGA GAAAGGCTGT TTGAAGCATC AGATCCGTAT CAGGTTCACG 
     4381 TTTGTAATCT TTGTGGAATA ATGGCAATTG CTAACACCAG GACTCACACA TACGAATGCA 
     4441 GGGGATGCCG CAATAAGACC CAGATCTCCT TGGTGCGAAT GCCGTATGCG TGCAAGCTGT 
     4501 TGTTCCAGGA GCTCATGTCC ATGAGCATCG CACCACGGAT GATGAGTGTT ACGCGTACGC 
     4561 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4621 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4681 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4741 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4801 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4861 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4921 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4981 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5041 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5101 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5161 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5221 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5281 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5341 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5401 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5461 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5521 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5581 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5641 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5701 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5761 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5821 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5881 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     5941 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     6001 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6061 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6121 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6181 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6241 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6301 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6361 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6421 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6481 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6541 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6601 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6661 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6721 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6781 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6841 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6901 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6961 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     7021 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7081 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7141 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7201 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7261 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7321 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7381 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7441 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7501 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7561 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7621 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7681 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7741 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7801 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7861 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7921 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7981 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     8041 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     8101 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8161 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8221 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8281 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8341 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8401 TTT
//