LOCUS       RR203190                5882 bp    DNA     circular  SYN 
DEFINITION  Tacstd2 (Myc-DDK-tagged ORF) - Rat tumor-associated calcium signal transducer 2 (Tacstd2), (10 ug)
ACCESSION   NM_001009540
VERSION     NM_001009540.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2037..2128 
                     /label="Myc-DDK"  
     misc_feature    2158..2743 
                     /label="PolyA signal"  
     misc_feature    2215..2234 
                     /label="Restriction Enzyme"  
     misc_feature    3019..3691 
                     /label="ColE1"  
     misc_feature    4121..4915 
                     /label="Kan/Neo"  
     misc_feature    4950..5252 
                     /label="SV40 ori"  
     misc_feature    5338..5815 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2012..2019 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2012 
                     /label="Tacstd2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGAGGGGC TTGGATCTAG CACCGCTGCT ACTGCTACTG CTGGCGATGG 
     1081 TGGCCGGCTT TTGCACGGCC CAGATCAACT GCACATGCCC TACCAACAAG ATGACCGTCT 
     1141 GCAACTCTAA TGGCCCAGGT GGGGTCTGCC AATGTCGGGC AATCGGCTCG CAGGTGTTGG 
     1201 TCGACTGCTC CACGCTAACT TCCAAGTGCC TGCTACTCAA GGCGCGCATG AGCGCCCGAA 
     1261 AGAGCAGCCG CAGACTGGTG AATCCGAGCG AGCACGCGAT CTTGGACAAC GATGGCCTCT 
     1321 ACGACCCGGA GTGTGACGAC AAGGGCCGCT TCAAGGCGCG CCAGTGCAAC CAGACCTCGG 
     1381 TGTGCTGGTG CGTAAACTCG GTGGGCGTGC GCCGAACGGA CAAGGGAGAC CAGAGCCTGC 
     1441 GCTGCGACGA AGTGGTGCGA ACCCACCACA TCCTCATTGA GTTGCGCCAC CGCCCGACAG 
     1501 ACCGAGCCTT CAACCACTCT GACCTAGACT CCGAGCTGCG GCGGCTCTTC AAGGAACGCT 
     1561 ACAAGCTGCA CCCCAGCTTC CTGGCCGCGG TGCACTACGA AGAGCCCACC ATCCAGATCG 
     1621 AGCTGCAGCA GAACGCGTCA CAGAAGGGCC TGAGAGACGT GGACATCGCT GACGCCGCCT 
     1681 ACTACTTCGA AAGGGACATC AAAGGCGAGT CACTGTTCGT GGGCCGTCGC GGCCTGGACG 
     1741 TGCAGGTGCG TGGGGAGCCC CTGCATGTGG AGCGGACTCT CATCTACTAC CTGGACGAGA 
     1801 AGCCCCCCCA GTTCTCCATG AAGCGCCTCA CCACCGGCCT CATTGCCGTC ATCGCTGTCG 
     1861 TCGCGGTGGC ACTAGTGGCC GGTGTGGTGG TCTTGGTGGT CACCAACCGG AGGAAATCAG 
     1921 GAAAATACAA AAAGGTGGAG CTTAAGGAGC TAGGGGAGAT GAGAAGCGAA CCTAGCTTGA 
     1981 GCTTAAGGAG CTAGGGGAGA TGAGAAGCGA ACCTAGCTTG AGCGGACCGA CGCGTACGCG 
     2041 GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA 
     2101 CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG 
     2161 ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA 
     2221 CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG 
     2281 TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA 
     2341 AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT 
     2401 CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG 
     2461 AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA 
     2521 GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC 
     2581 CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC 
     2641 TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG 
     2701 GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG 
     2761 GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT 
     2821 CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG 
     2881 TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC 
     2941 CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA 
     3001 AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT 
     3061 CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG 
     3121 GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG 
     3181 CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT 
     3241 CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC 
     3301 AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC 
     3361 TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC 
     3421 GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT 
     3481 TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC 
     3541 TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG 
     3601 AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA 
     3661 ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT 
     3721 GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG 
     3781 GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA 
     3841 ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC 
     3901 GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA 
     3961 GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC 
     4021 CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT 
     4081 GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA 
     4141 ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA 
     4201 GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA 
     4261 ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA 
     4321 AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT 
     4381 CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT 
     4441 GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC 
     4501 GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA 
     4561 GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA 
     4621 GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA 
     4681 CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT 
     4741 CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC 
     4801 CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT 
     4861 CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT 
     4921 CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT 
     4981 CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT 
     5041 GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT 
     5101 AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG 
     5161 CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA 
     5221 TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT 
     5281 AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT 
     5341 ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA 
     5401 AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT 
     5461 GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG 
     5521 CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG 
     5581 GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC 
     5641 GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT 
     5701 GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT 
     5761 TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT 
     5821 TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT 
     5881 TT
//