LOCUS       RR203182                8271 bp    DNA     circular  SYN 
DEFINITION  Trpa1 (Myc-DDK-tagged ORF) - Rat transient receptor potential cation channel, subfamily A, member 1 (Trpa1), (10 ug)
ACCESSION   NM_207608
VERSION     NM_207608.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4428..4519 
                     /label="Myc-DDK"  
     misc_feature    4549..5134 
                     /label="PolyA signal"  
     misc_feature    4606..4625 
                     /label="Restriction Enzyme"  
     misc_feature    5410..6082 
                     /label="ColE1"  
     misc_feature    6512..7306 
                     /label="Kan/Neo"  
     misc_feature    7341..7643 
                     /label="SV40 ori"  
     misc_feature    7729..8206 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4410..4416 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4410 
                     /label="Trpa1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGCGCAGC TTGAGGAGGG TTCTGCGCCC CGAGGAAAGA AAGGAGGTCC 
     1081 AGGGCGTCGT CTATCGCGGC GTGGGGAAAG ACATGGACTG CTCCAAGGAA TCCTTTAAGG 
     1141 TGGACATTGA AGGAGATATG TGCAGATTAG AAGCCTTCAT CAAGAACCGA AGAAAACTAA 
     1201 GCAAGTACGA GGATGAAAAT CTCTGTCTTC TGCATCACGC CGCAGCCGAA GGTCAAGTTG 
     1261 AACTGATGCA ATTGATCATC AATGGCTCTT CCTGTGAAGC GCTGAATGTA ATGGATGATT 
     1321 ATGGAAATAC CCCACTACAT TGGGCTGCAG AAAAAAATCA AGTTGAAAGT GTGAAGTTTC 
     1381 TTCTCAGCCA AGGAGCAAAT CCAAACCTCC GAAATAGAAA CATGATGGCA CCCCTTCACA 
     1441 TAGCTGTACA GGGCATGTAC AACGAAGTGA TCAAGGTCTT GACCGAGCAC AAGGCCACTA 
     1501 ACATCAATTT AGAAGGAGAG AATGGGAACA CAGCTTTGAT GTCCACGTGT GCCAAAGACA 
     1561 ACAGTGAAGC TTTGCAAATT TTGTTAGAAA AAGGAGCTAA GCTGTGTAAA TCAAATAAAT 
     1621 GGGGAGACTA CCCTGTGCAC CAGGCAGCAT TTTCAGGTGC CAAAAGATGC ATGGAATTAA 
     1681 TCTTAGCATA TGGTGAAAAG ACCGGCTATA GCCGGGAGGC TCACATTAAC TTTGTGAATC 
     1741 ATAAGAAAGC CAGCCCTCTC CACCTCGCAG TTCAGAGCGG CGACTTGGAC ATGATTAAGA 
     1801 TGTGCCTGGA CAGCGGTGCA CACATCGACA TGATGGAGAA TGCCAAATGC ATGGCCCTCC 
     1861 ATTTTGCTGC AACCCAGGGA GCCACTGACA TCGTTAAACT CATGATCTCA TCCTATACTG 
     1921 GAAGCAGCGA TATCGTGAAT GCAGTCGATG GCAATCAGGA GACCCTGCTT CACAGAGCCT 
     1981 CATTATTTGA TCATCATGAC CTGGCAGACT ACCTAATTTC AGTGGGAGCA GACATCAACA 
     2041 GCACTGATTC TGAAGGACGC TCTCCACTTA TTTTAGCAAC TGCTTCTGCA TCCTGGAATA 
     2101 TTGTGAATTT GCTCCTCTCT AAAGGTGCCA AAGTAGACAT AAAAGATCAT CTTGGGCGTA 
     2161 ACTTTTTACA TTTGACTGTG CAGCAGCCTT ATGGGCTAAG GAATTTGCGG CCTGAGTTTT 
     2221 TGCAGATGCA ACACATCAAG GAGCTGGTGA TGGATGAGGA CAATGATGGA TGTACGCCTC 
     2281 TCCATTATGC TTGTAGGCAG GGGGCCCCTG TCTCTGTAAA TAACCTCCTC AGGTTCAATG 
     2341 TGTCCGTTCA TTCCAAAAGC AAAGATAAGA AGTCTCCCCT GCACTTTGCC GCCAGCTATG 
     2401 GGCGCATCAA TACATGTCAG AGGCTTCTGC AAGACATCAG CGACACAAGG CTTTTGAATG 
     2461 AAGGGGATCT CCATGGAATG ACCCCTCTCC ATCTGGCAGC AAAGAATGGA CATGATAAAG 
     2521 TCGTTCAACT TCTTCTCAAG AAAGGGGCCT TGTTTCTTAG TGACCACAAT GGCTGGACTG 
     2581 CTTTGCATCA TGCGTCCATG GGTGGCTACA CTCAGACCAT GAAGGTCATT CTTGACACTA 
     2641 ACTTGAAGTG CACAGACCGG CTAGATGAAG AAGGGAACAC AGCACTTCAC TTTGCAGCAC 
     2701 GGGAAGGCCA TGCAAAGGCT GTTGCGATGC TTTTGAGCTA CAATGCTGAC ATCCTCCTGA 
     2761 ACAAGAAGCA AGCTTCCTTT CTGCATATTG CACTGCACAA TAAGCGCAAG GAAGTGGTTC 
     2821 TCACAACCAT CAGGAGTAAA AGATGGGATG AGTGTCTTCA AGTTTTTACT CATGATTCTC 
     2881 CAAGCAATCG CTGTCCAATC ATGGAAATGG TGGAATACCT CCCCGAGTGC ATGAAAGTTC 
     2941 TTCTAGATTT CTGCATGATA CCTTCCACAG AAGACAAGTC CTGCCAAGAC TACCATATTG 
     3001 AGTATAATTT CAAGTATCTC CAATGCCCAT TATCCATGAC CAAAAAAGTA ACCCCCACCC 
     3061 AGGATGTGAT CTATGAGCCT CTTACAATCC TCAATGTCAT GGTCCAACAT AACCGCATAG 
     3121 AGCTCCTCAA CCACCCTGTG TGTAGGGAAT ACTTACTTAT GAAATGGTGT GCCTATGGCT 
     3181 TCAGAGCTCA TATGATGAAC CTAGGATCTT ATTGTCTTGG TCTCATACCC ATGACCCTTC 
     3241 TTGTTGTCAA AATACAGCCT GGAATGGCCT TCAATTCTAC TGGAATAATC AATGAAACTA 
     3301 TTAGTACTCA TGAGGAAAGA ATAAACACTC TGAATTCGTT TCCATTAAAA ATATGTATGA 
     3361 TTCTAGTTTT TTTATCAAGT ATATTTGGAT ATTGCAAAGA AGTGGTCCAA ATTTTCCAAC 
     3421 AGAAAAGGAA CTACTTTCTG GACTACAACA ATGCTCTGGA GTGGGTCATC TACACCACCA 
     3481 GTATGATCTT CGTGTTGCCC TTATTCCTCG ACATCCCCGC GTATATGCAG TGGCAATGTG 
     3541 GAGCGATAGC AATATTCTTC TACTGGATGA ACTTCCTACT ATATCTTCAA AGGTTTGAGA 
     3601 ACTGTGGCAT TTTCATTGTT ATGTTGGAGG TGATTTTTAA AACATTGCTG AGATCGACGG 
     3661 GAGTGTTTAT CTTCCTGCTA TTGGCTTTTG GCCTCAGCTT TTACGTCCTC CTGAATTTCC 
     3721 AAGATGCCTT CAGCACCCCG TTGCTTTCCT TAATCCAGAC GTTCAGTATG ATGCTGGGAG 
     3781 ACATCAATTA CCGAGATGCT TTCCTAGAAC CGTTGTTCAG GAATGAGTTG GCATACCCGG 
     3841 TCCTCACCTT TGGGCAGCTT ATTGCCTTCA CAATGTTCGT CCCAATTGTT CTCATGAACC 
     3901 TACTGATTGG TTTGGCAGTT GGGGACATTG CTGAGGTCCA GAAGCATGCA TCATTAAAGA 
     3961 GGATTGCTAT GCAGGTGGAA CTGCATACCA ACTTAGAAAA AAAGCTACCA TTCTGGTACT 
     4021 TGCGCAAAGT GGATCAGAGG TCCACCATCG TGTATCCGAA TAGACCCAGG CACGGCAGGA 
     4081 TGCTGCGGTT TTTTCATTAC TTTCTTAGTA TGCAAGAAAC ACGACAAGAA GCACCAAACA 
     4141 TTGACACATG TTTGGAAATG GAAATACTGA AACAGAAATA CCGGCTGAAG GACCTCACTT 
     4201 CTCTTTTGGA AAAGCAGCAC GAGCTCATCA AACTCATCAT CCAAAAGATG GAGATCATCT 
     4261 CGGAGACAGA AGATGAAGAT AACCATTGCT CTTTCCAAGA CAGGTTCAAA AAGGAACGGC 
     4321 TAGAACAAAT GCATAGCAAA TGGAATTTTG TCTTAAACGC AGTTAAGACT AAAACACATT 
     4381 GTTCTATTAG CCACCCAGAC ATCAGCCACC CAGACATCAC GCGTACGCGG CCGCTCGAGC 
     4441 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4501 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4561 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4621 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4681 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4741 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4801 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4861 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4921 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4981 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5041 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5101 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5161 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5221 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5281 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5341 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5401 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5461 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5521 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5581 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5641 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5701 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5761 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5821 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     5881 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     5941 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6001 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6061 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6121 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6181 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6241 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6301 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6361 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6421 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6481 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6541 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6601 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6661 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6721 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6781 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6841 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6901 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     6961 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7021 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7081 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7141 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7201 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7261 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7321 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7381 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7441 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7501 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7561 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7621 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7681 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7741 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7801 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7861 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7921 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     7981 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8041 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8101 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8161 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8221 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//