LOCUS       RR203082                8442 bp    DNA     circular  SYN 
DEFINITION  Spag5 (Myc-DDK-tagged ORF) - Rat sperm associated antigen 5 (Spag5), (10 ug)
ACCESSION   NM_001044224
VERSION     NM_001044224.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4599..4690 
                     /label="Myc-DDK"  
     misc_feature    4720..5305 
                     /label="PolyA signal"  
     misc_feature    4777..4796 
                     /label="Restriction Enzyme"  
     misc_feature    5581..6253 
                     /label="ColE1"  
     misc_feature    6683..7477 
                     /label="Kan/Neo"  
     misc_feature    7512..7814 
                     /label="SV40 ori"  
     misc_feature    7900..8377 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4581..4587 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4581 
                     /label="Spag5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTGGAGGGTG AAAACACTGA ATCTCGGCTT GTCGCCTTCG CCCCAATCGG 
     1081 GGAAACCAGC TATGAGAACT CCTCTCCGAG AGCTTAAACT ACAGCCCGAG GCCCTCGCTG 
     1141 ATTCAGGGAA AGGTCCTATG TTCTCCTCGC TGACCCCATA CCTGCGCAGG CTGGATCTGA 
     1201 AGGAAAGGCG CAACAGCTCA TCTCCAGTGA ATTTTGTTAA TGCAGAGAAC TCATCTCCAG 
     1261 TGGATTTTGT CAATACTGAG AACACATCTC CAGTGGATTT TGACAATACT GAGAACAACT 
     1321 TCCTTTCAGA ACAGTTCAGC CATCCTTCAG AGTACATAGA AGCTTGTCAG CGTGAATCAG 
     1381 ATCTAACTCC TGAAAGCAGC TCTCCTTTCA ACACACTGGA GAAGGCAGTG GAAGCAATGG 
     1441 ATGATTTTGT GGCAGAACCA ATAGATGACG GTATAGTTAA GACCATGGTT CTTTTACCCT 
     1501 TTTCCTTAGG GCAGCAGCAA GACCTGATGC TCCAGGCTCA CTTAGATACC ACAGCAGAGA 
     1561 GAAATAAAAG CTCTCTAAAA GAATCTTTGG GGCTAGAAGA CCTGGTGGAA AAGGAGGCGG 
     1621 CACCTTGTGT GGAAGACAGC TTACCAGAAA CTGTTGCTAT CAGGCCTGAG CAACCCATCT 
     1681 CTCAGGACCC TCCATTAAGA GACAGTAACA CTGAAGCTGC ACCTGCGGGC TCAGTTCCTT 
     1741 CTGAAAATGT CTTGGGTTTC ACTGTGGCTT GTCTCTCTCC CTCAACTGTC TTGACCAAAG 
     1801 ATTTCTCTGT CGATCATGTC GATCCTGGGG AGGAAACTGC AGAGAACAGA GTTATACAGG 
     1861 AAGTGGAAAA GAGTTTTCTC GCACTCCCTG AGGAGGCTGG ATTGGGAGAT CAGGCACTTG 
     1921 CTGTAAATGC AGAAGACGTC TCATCCTTAT ACCTGTCATC AAGTCTAGTA GAAATGGGAC 
     1981 CCCAGGAGGC TCCAGGCCCA ACAGCAGAAG ATGCCAGTAG GATTCCTGAC TTGGAGTCAG 
     2041 AGACTTGGAT GTCCCCATTG GCCTGGCTAG AAAAAGGTGT GAACACGTCA GTCATGCTAC 
     2101 AAAGTCTCCG CCAGAGTTTA CCCTTTTCAA AATCTGTGCT TCAGGATGCT GCCGTTGGCA 
     2161 ATACACCCCA CTCCACTTAT TCTGTGGGAA CTTCCTTTAC TCCTCCAGTG CCACTGGAAG 
     2221 TAGGCACCAA GGACAGTACT TCAGAGACAG AGCGCCTCCT CTTGGGCTGC CGTCCTCCAG 
     2281 ATCTGGCAGC CTTGTCCCGG CACGACTTGG AAGAGAACCT GTTGAACGCC CTTGTCTTGC 
     2341 TGGAAGTTCT TTCTCACCAG CTGCAAGCCT GGAAGAGTCA GCTGACTGTC CCGAGCCGGC 
     2401 AAGCTCAAGA CAGCAGCACA CAGACCGACA GCTCTGCAGC TGTGGTCACT AAGACACCTA 
     2461 AACATCTTCA GGACAGCAAG GAGATTCGAC AGGCTCTACT GCAAGCCAGG AATGTCATAC 
     2521 ATTCATGGGG GCTAGTCTCT AGAGACCTGA TGTCCTTGCT TCACCTGTCC CTGACACATG 
     2581 TGCAAGATGA CAGAGTGACA GTGAGTCAGG AGTCTCAGCG TGCAGAGACC CTGGTTTCCT 
     2641 CCTGTTCTCG TGTACTGAAG AAATTGAGGG CAAAGCTCCA GAGCCTCAAA ACAGAATGGG 
     2701 AGGAGGCACG GCACAGGGAG GAAATGGCCC TTAAAGGCAA GGATGCGGCA GAGGCAGTAC 
     2761 TGGAGGCTTT CCGTGCACAC GCTAGCCAGC GCATCAGCCA ACTGGAACAG GGTATCACAT 
     2821 CTGTGCAGGA ATTCAGAGGC CTTCTACAGG AGGCACAGAC CCAACTGATA GGGCTTCACA 
     2881 CAGAGCAAGA AGAGCTGGCT CAGCACACAG TGAGTCTGAC TTCTGCCTTG CAGCAGGACT 
     2941 GGACATCAGT CCAACAGAAT TATGGGACAT GGGCAGCTTT GCTGAGTCGG TCTCGAGAAC 
     3001 TCACCAAGAA ACTCACAGCC AAGAGCCGGC AAGCCCTTCA GGAACGTGAT GCTGCAATTG 
     3061 AGGAAAAGGA TCAGGTTGTG AAGGAAGTGG AACAAGTCTC TGCCCATTTA GAGGACTGTA 
     3121 AAGGCCAAAT AGAACAACTG AAGTTGGAAA ATAGTCGCCT TACTGCAGAT CTCTCGGCTC 
     3181 AGCTGCAGAC TCTGGCTAGC ACAGAGAACG AACTAAAGGA ACTACAGAGT CAGCATTCCC 
     3241 ATTGTGTCCA AGACTTGGCC ATGAAGGATG AACTGCTGTG TCAACTTACC CAGAGCAACA 
     3301 AGGAACAGGC TGCTCAGTGG CAAAAGGAAG AAACGGAACT GAAACACAGA CAAGCAGAAC 
     3361 TGCTGCAGCA GAAGGCTGTG TTGGCTAAGG AGGTACAGGA CCTGAGGGAG ACCATGGAGT 
     3421 TTGTAGATGA AGAGAGTCAA GTTGCTCACC TGGAGCTGGG CCAGATCGAG AGTCAGTTGA 
     3481 AAGCCACACT AGAAGTTCTG CGGGAGCGCA GCCTGCAGTG TGAGACCCTC AAGGACACCG 
     3541 TGGAGAGCCT GAGGGCAGAG CTGGCCAGCA CCGAAGCAAA GCATGAGCAA CAAGCGCTGG 
     3601 AGAAGACGCA CCAGCATTCT AAAGAGCTGT GTCTATTGGC GGAGCAGCTA CAGAGTCTCA 
     3661 CCCTCTTCTT AGAGGCTAAA CTAGAGGAGA ACAAGGCAGA ATCAGACATC ATTCTGCCCA 
     3721 GCACAGGCTG TGCTCCAGCC CAGGAACACC CTCCTTCCAG TGATAGCAGC GTCTCAGAAC 
     3781 AGATCCCGAC AGCAGTGGTA GATGAAGTGC CAGAACCAGC TCCTGTGCCA TTGCTTGGAA 
     3841 GTGTTAAGAG TGCTTTCACC CGCGTAGCCT CAACAGCTCC CTTTCGGCCT ACAGAGACCC 
     3901 CAGCCCTGGA GAAGAGCCTG GCAGAAATGA GTGCTGTGTT ACAGGAGCTT CAACGCCTCT 
     3961 GTGCCCTGCT GCAAGAATCT AAAGAGGAAG CCGTTGGTGT CCTGCAGAGG GAAATCTGTG 
     4021 AACTGCACAC AAGACTACAG GCCCAAGAAG AAGAACATCA GGAAGCCCAA AAGGCAAAGG 
     4081 AAGCAGACAT AGAGAAGTTG AACCAGGCCT TGTGCTTGCG CCACAAGAAT GAGAAGGAAC 
     4141 TCCTGGAAGT GATACAGAAG CAGAACGAAA AGATCCTGGG GCAGATAGAC ACGAGTGGCC 
     4201 AGCTCATAAA CCTCAGGGAG GAGGTGACCC AGCTTACACG TTTACTTCGG CGGGCAGAGA 
     4261 CAGAGACTAA AGTGCTCCAG GAAGCCCTGG AAGGCCAGGT AGATCCCAGC TGTCAGCTGA 
     4321 TGGCTACTAA CTGGATCCAG GAAAAAGTGT TTCTCTCGCA GGAGGTGAAC AAACTGAGGG 
     4381 CTATGTTCCT GGAGGTGAAA AATGAGAAGA AACAGCTGAT GGACAAGTAT CTGAGCCATA 
     4441 GGCACATCCT GGAGGAGAAT CTTCGGCGCT CTGACACAGA GTTAAAGAAA CTTGATGACA 
     4501 CAATTCAGCA TATCTATGAG ACTCTGTTGT CTATCCCAGA GGTAGTGAAG AGTTGCAAGG 
     4561 AGCTACAAGG ATTGCTAGAA TTTCTGAGCA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4621 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4681 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4741 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4801 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4861 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4921 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4981 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     5041 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5101 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5161 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5221 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5281 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5341 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5401 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5461 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5521 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5581 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5641 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5701 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5761 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5821 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5881 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5941 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     6001 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6061 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6121 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6181 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6241 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6301 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6361 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6421 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6481 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6541 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6601 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6661 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6721 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6781 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6841 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6901 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6961 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     7021 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     7081 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7141 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7201 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7261 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7321 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7381 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7441 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7501 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7561 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7621 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7681 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7741 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7801 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7861 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7921 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7981 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     8041 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     8101 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8161 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8221 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8281 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8341 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8401 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//