LOCUS       RR203065                8694 bp    DNA     circular  SYN 
DEFINITION  Pfas (Myc-DDK-tagged ORF) - Rat phosphoribosylformylglycinamidine synthase (Pfas), (10 ug)
ACCESSION   NM_001105791
VERSION     NM_001105791.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4851..4942 
                     /label="Myc-DDK"  
     misc_feature    4972..5557 
                     /label="PolyA signal"  
     misc_feature    5029..5048 
                     /label="Restriction Enzyme"  
     misc_feature    5833..6505 
                     /label="ColE1"  
     misc_feature    6935..7729 
                     /label="Kan/Neo"  
     misc_feature    7764..8066 
                     /label="SV40 ori"  
     misc_feature    8152..8629 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4833..4839 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4833 
                     /label="Pfas"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGTTGCTC GGGAGTCCTG GCTGGTCCCT GGCTCCAATG ATTTGTTGCT 
     1081 GGAGGTGGGA CNNGCTGAAT TTCTCTACTC CAGCATCGAC CAACATTGTT TCTGTGTGCC 
     1141 AGGCTGCTGG GCTGAGAGCT GTGGATCGGG TAGAGACCAC CCGGCGCTAC CGGCTTTCGT 
     1201 TTGCCAAGCA GCCTACAGCT GAAATGGAAG CCATTTCTCT GGCTGCCTTG CATGACCGGA 
     1261 TGACAGAGCA GCACTACCCT GACCCCATTC AGAGCTTCTC TCCTCAGAGC ATCCCAGCCC 
     1321 CACTCAAGGA CTCCATCAAC ATCCTAGCCG AGGGTCGGCC TGCCCTGGAG AAGGCCAACC 
     1381 AGGAGCTAGG TTTGGCTCTT GACTCCTGGG ACCTCGATTT CTACACCAAG CGCTTTCAAG 
     1441 AGCTGCAGCG AAACCCCAGT ACAGTGGAGG TCTTTGACTT GGCTCAGTCC AATAGTGAGC 
     1501 ACAGCCGACA TTGGTTCTTC AAGGGCCAGC TCCATGTGGA CGGGAAGAAG CTAGCACGTT 
     1561 CCCTCTTTGA ATCCATCATG AGCACTCAGT CATCCTCCAA CCCTAACAAT GTCCTCAAGT 
     1621 TCTGTGACAA CAGCAGTGCA ATCCAAGGGA AGGAAGTCCG ATTCCTGCGG CCTGAGGACT 
     1681 CTACACGACC AAGCTGCTTC CGGCAACAGC GGGGGCTTAG ACATGTTGTC TTCACAGCAG 
     1741 AGACACATAA CTTCCCCACC GGAGTGGCGC CCTTCAGCGG TGCAACCACA GGCACAGGTG 
     1801 GCCGCATCAG AGATGTCCAG TGTACAGGCC GTGGAGCCCA CGTGGTGGCT GGCACTGCTG 
     1861 GCTATTGCTT TGGGAACCTG CATATCCCAG GTTATAATCT GCCCTGGGAG GATCCAAGCT 
     1921 TCCAGTATCC TGGGAACTTC GCCAGGCCTT TGGAAGTTGC TATTGAGGCC AGTAATGGAG 
     1981 CATCTGACTA TGGCAACAAG TTTGGAGAAC CAGTGCTGGC TGGATTTGCC CGCTCTTTGG 
     2041 GTCTCCAGCT TCCAGATGGT CAGAGACGTG AGTGGATCAA GCCCATCATG TTCAGTGGGG 
     2101 GCATTGGGTC CATGGAAGCC AAGCATGTAG GCAAAGAACC CCCAGAGCCA GGCATGGAGG 
     2161 TTGTGAAGGT CGGGGGTCCC GTCTACAGGA TTGGAGTTGG GGGTGGAGCT GCTTCGTCCG 
     2221 TGCAGGTTCA GGGAGACAAC ACCAGTGACC TGGACTTCGG AGCTGTGCAG CGGGGAGATC 
     2281 CGGAAATGGA GCAGAAGATG AATCGTGTGA TCCGGGCTTG TGTGGAGGCC CCAGGAGGAA 
     2341 ACCCCATATG CAGCCTCCAT GACCAGGGTG CTGGTGGCAA TGGCAATGTC CTAAAGGAGC 
     2401 TGAGTGAGCC AGCGGGAGCT GTCATTTACA CCAGCCGCTT TCAGCTGGGT GATCCAACCT 
     2461 TGAATGCACT GGAAATCTGG GGGGCTGAGT ACCAGGAGTC AAATGCACTT CTTCTGAGGC 
     2521 CCTCTGACCG GGACTTTCTG AGTCGCGCCA GTGCCCGGGA ACGCTGCCCA GCTTGTTTTG 
     2581 TGGGCACAAT CACTGGAGAC AAGAGAATCG TGCTAGTGGA CGACCGCGAA TGCTTGATGG 
     2641 GAAAAAGTGG CCAGGGAGAT GCTCCCCCGA CACCTCCAAC TCCTGTGGAC CTGGACCTTG 
     2701 ACTGGGTTCT GGGAAAGATG CCTCAGAAGG AGTTCTTCCT CCAGAGAAAA CCCCCTGTGC 
     2761 TGCAGCCTTT GGCCTTGCCC CCCGACCTGA GTGTGGGCCA GGCTCTAGAA CGGGTTCTAA 
     2821 GGCTGCCTGC TGTGGCCAGC AAGCGCTACC TCACTAACAA GGTGGATCGC TCTGTGGGTG 
     2881 GCCTTGTTGC CCAACAGCAG TGTGTCGGGC CCCTGCAGAC TCCTCTGGCT GATGTGGCTG 
     2941 TTGTAGCACT GAGTCACCAG GAGCTTGTAG GGGCCGCCAC AGCCCTGGGA GAGCAGCCAG 
     3001 TCAAGAGCCT GCTGGACCCC AAGGCTGCTG CCAGGCTAGC CGTGTCTGAG GCCCTCACCA 
     3061 ACCTGGTATT CGCTCTGGTC ACTGATCTCC GAGATGTGAA GTGCAGTGGG AACTGGATGT 
     3121 GGGCAGCCAA GCTCCCGGGT GAGGGTGCAG GGCTAGCCGA TGCCTGTGAG GCTATGGTGA 
     3181 CAGTGATGGC AGCACTGGGT GTGGCAGTAG ATGGTGGCAA GGACTCCCTC AGCATGGCTG 
     3241 CCCGGGTCGG CACTGAGACC GTGCCAGCTC CTGGGTCACT GGTCATTTCA GCATATGCTG 
     3301 TCTGTCCAGA CATCACAGCC ACTGTGACCC CAGACCTCAA GCATCCTGGA GGAAAAGGTC 
     3361 ATTTGCTCTA TGTGCCTTTG AGCCCTGGGC AGCACCGGCT GGGAGGAACG GCTCTGGCCC 
     3421 AATGTTTCTC TCAGCTCGGG GAACACCCTC CTGATCTAGA CCTTCCCGAG AACCTCGTGC 
     3481 GTGCCTTTCA TATCACCCAG GGGCTGCTGA AAGGTGAGTG GGGCCCTGAT CGCCGTCTTT 
     3541 GCTCAGGCCA TGATGTCAGC GATGGAGGGC TTGTAACCTG CCTTCTAGAG ATGGCCTTTG 
     3601 CTGGGAATTG TGGGATAGAG GTAGATGTAC CTGCCCCTGG GATCCATGCA TTGCCTGTGC 
     3661 TGTTCGCAGA AGAGCCAGGT CTGGTGTTGG AAGTACAGGA GGCAGATGTA GCCGGAGTAC 
     3721 TGCAGAGGTA CCAGAGTGCT GGCCTACACT GTCTGGAACT AGGCCACACT GGCGAGGCTG 
     3781 GACCCCAGGC CATGGTCCGA GTGTCAGTGA ATGGGACTGT GGTTGTAGAG GAGCCTGTTG 
     3841 GGCAGTTGAG AGCCCTCTGG GAGGAAACAA GTTTCCAGCT GGACCTACTG CAGGCAGAGC 
     3901 CACGCTGTGT GACAGAGGAG AAACAGGGCC TGAAGGAACG GATAGGACCT AGCTACTGCC 
     3961 TGCCGCCTAC CTTTCCTGTG GCCTCTGTGC CCTGTAAACC TGGTGGTCCC ATCCCTCGAG 
     4021 TTGCCATCTT GCGTGAAGAG GGCAGCAATG GAGACCGAGA GATGGCTGAT GCTTTTCACT 
     4081 TGGCTGGCTT TGAGGTGTGG GATGTGACCA TGCAGGACCT CTGCTCTGGG GCCGTCAGGC 
     4141 TGGACACGTT CCGTGGTGTG GCCTTTGTGG GTGGCTTCAG CTACGCAGAT GTCCTGGGCT 
     4201 CTGCCAAAGG TTGGGCAGCT GCAGTGACCT TCAATCCACA GGCCAGGGAA GAGCTGGGCC 
     4261 GCTTCCGCAG GCGGCCAGAC ACCTTCAGCC TGGGCGTGTG TAATGGCTGT CAGCTGCTGG 
     4321 CTCTTCTGGG CTGGGTGGGA AGTGATCCCA ATGAAGACCA AGTTGAGCCG GACCACGACT 
     4381 CCCAGCCAGC ACAGCCAGGC CTCCTGCTCC GTCACAATCT CTCTGGGCGG TTTGAGTCCC 
     4441 GATGGGCCAC TGTACGTGTG GAGCCGGGGC CAGCCCTGAT GCTTCGAGGG ATGGAAGGCT 
     4501 CTGTGCTGCC TGTGTGGAGT GCTCATGGGG AAGGTTACAT GGCATTTTCT TCTCCTGAAC 
     4561 TTCAAGCCAA GATCAAGGCC AAGGGCTTGG TTCCTCTGCA CTGGGCAGAT GATGATGGGA 
     4621 ACCCCACAGA ACAGTACCCT TTGAACCCCA ATGGATCTCC AGGGGGCATA GCTGGCATCT 
     4681 GCTCCCCAGA TGGTCGCCAT CTGGCACTCA TGCCACACCC TGAGCGGGCT GTGAGGCTTT 
     4741 GGCAGTGGGC ATGGCGACCC TCTCCCTTTG ATGGTCTTTC CACCTCCCCG TGGCTGCAGC 
     4801 TCTTCATCAA TGCTCGGAAC TGGACCCAGG AGGACAGCTG CACGCGTACG CGGCCGCTCG 
     4861 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4921 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4981 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     5041 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     5101 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5161 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5221 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5281 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5341 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5401 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5461 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5521 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5581 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5641 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5701 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5761 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5821 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5881 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5941 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     6001 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     6061 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6121 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6181 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6241 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6301 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6361 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6421 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6481 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6541 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6601 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6661 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6721 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6781 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6841 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6901 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6961 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     7021 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     7081 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7141 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7201 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7261 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7321 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7381 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7441 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7501 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7561 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7621 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7681 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7741 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7801 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7861 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7921 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7981 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     8041 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     8101 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8161 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8221 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8281 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8341 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8401 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8461 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8521 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8581 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8641 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//