LOCUS       RR203025                9297 bp    DNA     circular  SYN 
DEFINITION  A2m (Myc-DDK-tagged ORF) - Rat alpha-2-macroglobulin (A2m), (10 ug)
ACCESSION   NM_012488
VERSION     NM_012488.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5454..5545 
                     /label="Myc-DDK"  
     misc_feature    5575..6160 
                     /label="PolyA signal"  
     misc_feature    5632..5651 
                     /label="Restriction Enzyme"  
     misc_feature    6436..7108 
                     /label="ColE1"  
     misc_feature    7538..8332 
                     /label="Kan/Neo"  
     misc_feature    8367..8669 
                     /label="SV40 ori"  
     misc_feature    8755..9232 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5436..5442 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5436 
                     /label="A2m"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGAAGCAC AGGCTCCGGA GCCTGGCCCT GCTGCCACTG CTGCTGCGGC 
     1081 TGCTGCTGCT GCTGCTGCCC ACCGATGCCT CAGCTCCACA AAAACCAATC TACATGGTGA 
     1141 TGGTTCCCTC CCTGCTCCAC GCAGGCACCC CCGAGAAGGC CTGCTTCCTG TTCAGCCATC 
     1201 TAAACGAGAC AGTGGCTGTG AGAGTGTCCT TGGAGTCTGT CCGTGGGAAC CAAAGCCTCT 
     1261 TCACTGACCT TGTAGTTGAC AAGGACCTAT TCCACTGTAC CTCCTTCACC GTCCCACAGT 
     1321 CTTCATCCGA TGAGGTGATG TTTTTCACTG TCCAAGTAAA AGGAGCAACT CATGAGTTCA 
     1381 GGAGGCGGAG CACGGTGCTG GTTAAGAAGA AAGAGAGCCT GGTCTTTGCT CAGACTGACA 
     1441 AGCCCATCTA CAAACCAGGA CAGACAGTGA GATTTCGTGT TGTCTCATTG GACGAAAGTT 
     1501 TCCATCCCCT TAATGAATTG ATTCCTCTAC TGTACATTCA GGATCCCAAA AACAATCGCA 
     1561 TTGCACAATG GCAGAATTTC AATTTAGAGG GTGGCCTCAA ACAGCTGTCC TTCCCCCTCT 
     1621 CCTCAGAGCC CACTCAGGGC TCCTACAAGG TGGTGATACG TACAGAATCA GGCAGGACCG 
     1681 TCGAGCACCC TTTCTCTGTG GAGGAATTCG TGCTTCCCAA GTTCGAAGTG AGAGTGACAG 
     1741 TTCCAGAAAC AATCACCATC CTGGAGGAAG AGATGAATGT GTCCGTGTGT GGAATATACA 
     1801 CCTATGGGAA GCCTGTTCCA GGACGTGTGA CTGTAAACAT TTGCAGAAAG TACAGTAATC 
     1861 CTTCTAACTG CTTCGGCGAA GAGTCCGTGG CTTTCTGTGA GAAACTCAGC CAACAGTTAG 
     1921 ACGGCCGTGG CTGCTTCTCA CAGCTAGTGA AAACCAAGTC CTTCCAGCTA AAGAGACAAG 
     1981 AGTATGAGAT GCAGCTCGAT GTACATGCCA AGATCCAAGA AGAAGGAACA GGTGTGGAAG 
     2041 AAACTGGAAA GGGGCTCACT AAGATCACAA GAACCATAAC CAAACTATCA TTTGTGAACG 
     2101 TGGATTCACA TTTCAGACAA GGAATTCCTT TCGTTGGACA GGTGCTCCTG GTGGATGGGA 
     2161 GAGGCACCCC TATTCCGTAT GAAACGATCT TCATTGGGGC GGATGAAGCA AACCTGTACA 
     2221 TAAATACAAC CACTGATAAG CACGGCCTGG CGAGGTTCTC CATCAACACC GATGACATCA 
     2281 TGGGCACGTC CCTAACTGTC AGGGCCAAAT ACAAGGATAG CAACGCCTGC TATGGATTCA 
     2341 GATGGTTGAC AGAAGAGAAT GTAGAGGCTT GGCACACTGC CTACGCTGTT TTCTCACCAA 
     2401 GCAGAAGCTT CCTGCACCTG GAATCCCTGC CTGATAAACT GCGCTGTGAC CAAACCCTGG 
     2461 AGGTCCAGGC ACATTACATT CTAAATGGCG AGGCCATGCA GGAGCTGAAG GAGCTCGTCT 
     2521 TCTACTATCT GATGATGGCC AAGGGAGGCA TCGTACGGGC GGGGACTCAC GTTCTGCCCC 
     2581 TGAAGCAGGG ACAAATGAGA GGTCACTTTT CCATACTCAT CTCGATGGAG ACAGACCTGG 
     2641 CTCCCGTGGC TCGACTGGTC CTCTATGCCA TCCTACCCAA TGGAGAAGTG GTTGGAGACA 
     2701 CTGCTAAATA TGAGATTGAG AACTGCCTGG CTAACAAGGT GGATTTGGTC TTCCGCCCGA 
     2761 ATAGCGGTCT TCCAGCTACC CGTGCCCTCC TTAGTGTCAT GGCTTCTCCT CAGTCCCTTT 
     2821 GTGGCCTGCG AGCTGTGGAC CAAAGCGTGC TGCTCATGAA ACCTGAGACT GAGCTCTCCG 
     2881 CATCCCTGAT TTATGACCTG CTACCAGTGA AAGACCTCAC TGGCTTCCCT CAGGGTGCGG 
     2941 ATCAACGGGA AGAAGACACT AATGGCTGCG TTAAGCAAAA TGACACTTAC ATTAATGGAA 
     3001 TCCTGTACTC GCCAGTGCAG AATACAAATG AAGAGGACAT GTACGGCTTC CTAAAGGATA 
     3061 TGGGCTTAAA GGTATTTACC AACTCGAACA TCCGTAAACC CAAAGTCTGC GAACGGCTCA 
     3121 GAGACAATAA AGGAATACCA GCTGCGTACC ACCTCGTAAG CCAAAGCCAC ATGGACGCTT 
     3181 TTCTAGAGTC TTCAGAGTCT CCCACAGAGA CTAGGCGAAG CTACTTCCCT GAGACGTGGA 
     3241 TCTGGGACTT GGTGGTGGTG GACTCAGCAG GAGTGGCTGA AGTGGAAGTG ACAGTCCCTG 
     3301 ACACCATCAC TGAATGGAAG GCCGGGGCCT TCTGCCTGTC TAATGACACT GGTCTGGGCC 
     3361 TGTCTCCTGT GGTCCAATTC CAAGCCTTCC AGCCCTTCTT CGTGGAGCTC ACAATGCCCT 
     3421 ACTCCGTGAT CCGTGGAGAA GCCTTCACGC TCAAGGCCAC TGTGCTGAAC TACCTCCCTA 
     3481 CATGCATCCG GGTTGCCGTG CAGCTGGAGG CCTCTCCCGA TTTTCTGGCT GCCCCAGAGG 
     3541 AGAAGGAACA AAGGTCTCAC TGCATCTGTA TGAACCAGCG GCACACCGCG TCCTGGGCAG 
     3601 TGATCCCCAA GTCATTAGGA AATGTGAATT TCACAGTTAG TGCAGAGGCA CTGAACTCTA 
     3661 AGGAGCTGTG TGGGAATGAG GTACCGGTGG TCCCTGAACA GGGCAAAAAA GACACGATCA 
     3721 TCAAGTCCCT GCTGGTTGAA CCCGAAGGTC TAGAGAACGA AGTGACATTT AACAGTCTGC 
     3781 TTTGTCCAAT GGGTGCTGAG GTATCTGAAC TGATAGCCCT GAAGCTGCCA TCAGACGTGG 
     3841 TAGAGGAATC TGCCAGAGCC TCTGTCACAG TTTTGGGAGA TATATTGGGT TCTGCCATGC 
     3901 AGAATACACA GGATCTCCTC AAGATGCCCT ATGGCTGTGG AGAACAGAAC ATGGTTCTAT 
     3961 TTGCTCCTAA TATCTATGTC CTGGACTATC TGAATGAAAC ACAGCAGCTG ACACAGGAGA 
     4021 TCAAGACCAA GGCCATTGCC TATCTCAATA CGGGCTACCA AAGACAATTA AACTACAAGC 
     4081 ACCGGGATGG CTCCTACAGC ACCTTTGGGG ATAAACCTGG CAGGAATCAT GCCAATACCT 
     4141 GGCTCACAGC CTTTGTACTG AAGAGTTTTG CTCAGGCTCG AAAATATATC TTCATCGATG 
     4201 AAGTACACAT CACCCAAGCC CTCTTATGGC TCTCTCAGCA GCAGAAGGAC AATGGTTGTT 
     4261 TCAGGAGCTC CGGGTCACTG CTCAACAATG CCATGAAGGG AGGAGTAGAA GATGAAGTCA 
     4321 CCTTGTCTGC CTACATCACC ATAGCTCTCC TGGAGATGTC TCTTCCTGTC ACTCATCCTG 
     4381 TTGTCCGCAA TGCCCTCTTT TGCCTGGACA CAGCCTGGAA GTCAGCAAGG GGAGGAGCTG 
     4441 GTGGCAGCCA TGTCTACACT AAGGCGCTGT TGGCCTATGC ATTTGCCCTT GCTGGTAACC 
     4501 AGGACACGAA GAAGGAGATC CTGAAATCAC TCGATGAGGA GGCTGTAAAA GAAGAAGATT 
     4561 CTGTCCACTG GACCAGACCT CAGAAACCCA GCGTGTCAGT GGCCCTCTGG TACCAACCCC 
     4621 AGGCTCCCTC GGCTGAGGTA GAGATGACTG CATATGTGCT CCTGGCTTAT CTTACCACTG 
     4681 AGCCAGCTCC AACCCAAGAG GACCTAACGG CTGCCATGCT CATCGTGAAG TGGCTCACAA 
     4741 AGCAGCAGAA TTCCCACGGT GGCTTCTCTT CCACCCAGGA CACTGTAGTG GCTCTCCACG 
     4801 CTTTGTCCAA ATACGGGTCC GCCACTTTCA CAAGAGCTAA GAAAGCTGCA CAGGTGACCA 
     4861 TCCATTCTTC GGGCACATTT TCTACAAAAT TCCAAGTCAA CAACAACAAC CAATTATTAC 
     4921 TCCAGAGAGT CACATTGCCG ACTGTGCCTG GGGATTACAC CGTGAAGGTG ACAGGAGAAG 
     4981 GCTGTGTCTA TCTCCAGACA TCCTTGAAAT ACAGTGTTCT CCCGAGAGAG GAGGAGTTCC 
     5041 CCTTCACTGT GGTGGTGCAG ACTCTGCCTG GGACATGTGA GGATCCCAAA GCTCACACCA 
     5101 GCTTCCAGAT CTCACTCAAC ATCAGTTACA CTGGAAGCCG TTCTGAATCC AACATGGCAA 
     5161 TTGCTGACGT GAAGATGGTG TCCGGCTTCA TCCCCTTGAA ACCAACAGTG AAAATGCTTG 
     5221 AAAGATCTGT GCATGTGAGC CGAACAGAAG TCAGCAATAA CCATGTCTTG ATTTACCTGG 
     5281 ATAAGGTGTC AAATCAGACG GTGAACTTGT CCTTCACGGT TCAGCAAGAT ATTCCAATAA 
     5341 GAGACCTGAA GCCAGCCGTA GTGAAAGTCT ACGATTACTA TGAGAAAGAT GAGTTTGCAG 
     5401 TTGCAAAATA CAGCGCTCCC TGCAGCACAG ATTATGGAAA TGCCACGCGT ACGCGGCCGC 
     5461 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     5521 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     5581 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     5641 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     5701 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     5761 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     5821 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     5881 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5941 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     6001 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     6061 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     6121 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     6181 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     6241 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     6301 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     6361 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     6421 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     6481 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     6541 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     6601 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     6661 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     6721 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     6781 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     6841 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6901 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6961 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     7021 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     7081 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     7141 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     7201 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     7261 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     7321 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     7381 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     7441 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     7501 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     7561 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     7621 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     7681 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     7741 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     7801 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     7861 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7921 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7981 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     8041 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     8101 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     8161 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     8221 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     8281 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     8341 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     8401 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     8461 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     8521 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8581 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     8641 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     8701 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     8761 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     8821 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     8881 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8941 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     9001 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     9061 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     9121 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     9181 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     9241 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//