LOCUS       RR203000                7230 bp    DNA     circular  SYN 
DEFINITION  Cul5 (Myc-DDK-tagged ORF) - Rat cullin 5 (Cul5), (10 ug)
ACCESSION   NM_022683
VERSION     NM_022683.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3385..3476 
                     /label="Myc-DDK"  
     misc_feature    3506..4091 
                     /label="PolyA signal"  
     misc_feature    3563..3582 
                     /label="Restriction Enzyme"  
     misc_feature    4367..5039 
                     /label="ColE1"  
     misc_feature    5469..6263 
                     /label="Kan/Neo"  
     misc_feature    6298..6600 
                     /label="SV40 ori"  
     misc_feature    6686..7163 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3360..3367 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3360 
                     /label="Cul5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGACGTCT AATCTGTTAA AGAATAAAGG TTCTCTCCAG TTTGAAGACA 
     1081 AGTGGGATTT CATGCGTCCA ATTGTTTTGA AGCTTTTACG CCAGGAATCT GTAACAAAGC 
     1141 AGCAGTGGTT TGATCTGTTT TCGGATGTAC ATGCTGTCTG TCTCTGGGAT GATAAAGGCT 
     1201 CATCAAAAAT TCATCAGGCT TTAAAAGAAG ATATCCTTGA ATTTATTAAG CAAGCACAGG 
     1261 CACGCGTACT GAGCCATCAA GATGACACAG CTTTGCTAAA GGCATATATT GTTGAATGGC 
     1321 GAAAATTCTT CACCCAGTGC GATATTTTAC CAAAACCTTT TTGTCAACTA GAGGTGACTT 
     1381 TATTGGGTAA ACAAAGCAGC AATAAAAAAT CAAATATGGA AGACAGTATT GTTCGAAAGC 
     1441 TCATGCTTGA TACGTGGAAT GAGTCGATTT TTTCGAATAT AAAGAACAGA CTCCAGGATA 
     1501 GTGCAATGAA GCTGGTGCAT GCTGAGAGAT TAGGGGAAGC TTTTGATTCC CAGCTGGTCA 
     1561 TAGGAGTGCG AGAGTCCTAT GTTAATCTTT GTTCTAATCC TGAGGACAAG CTTCAGATCT 
     1621 ATAGGGATAA TTTTGAGAAG GCATATTTGG ACTCAACAGA GAGATTTTAT AGAACTCAGG 
     1681 CACCCTCATA TTTACAGCAA AATGGTGTAC AGAATTACAT GAAATATGCA GATGCTAAAT 
     1741 TAAAAGAAGA AGAAAAAAGA GCACTCCGAT ACTTAGAAAC AAGACGAGAA TGTAACTCTG 
     1801 TTGAAGCACT CATGGAATGC TGTGTGAACG CACTGGTGAC CTCATTTAAA GAGACTATTT 
     1861 TAGCAGAATG TCAAGGCATG ATCAAGCGAA ATGAAACTGA AAAGTTACAT TTGATGTTTT 
     1921 CACTGATGGA CAAAGTTCCT GGTGGGATCG AGCCCATGTT GAAGGATCTG GAGGAGCACA 
     1981 TCATAAGTGC TGGTCTAGCA GACATGGTGG CAGCTGCTGA AACCATCACC ACTGACTCTG 
     2041 AGAAGTATGT GGAACAATTA CTTACACTGT TCAATAGATT CAGTAAACTG GTCAAAGAAG 
     2101 CTTTTCAGGA TGATCCTCGT TTCCTTACTG CAAGAGATAA GGCGTATAAA GCAGTTGTTA 
     2161 ATGATGCTAC CATATTTAAA CTTGAATTGC CTTTGAAGCA AAAAGGAGTG GGGTTGAAAA 
     2221 CTCAGCCTGA GTCAAAGTGC CCTGAGTTGC TTGCTAATTA CTGTGACATG TTATTAAGGA 
     2281 AAACACCATT AAGCAAAAAA CTAACCTCTG AAGAGATTGA AGCAAAGCTT AAAGAAGTGC 
     2341 TCTTGGTACT TAAATATGTA CAAAACAAAG ATGTTTTTAT GAGGTATCAC AAAGCTCATC 
     2401 TTACCCGACG TCTCATATTG GACATCTCTG CTGATAGCGA GATTGAAGAA AACATGGTGG 
     2461 AGTGGCTAAG AGAAGTTGGT ATGCCAGCAG ATTATGTGAA CAAACTTGCT AGAATGTTTC 
     2521 AGGACATAAA AGTATCTGAA GACTTGAACC AAGCTTTTAA AGAAATGCAT AAAAATAATA 
     2581 AGTTGGCATT ACCAGCTGAT TCAGTTAATA TAAAGATTTT GAATGCTGGT GCTTGGTCTA 
     2641 GAAGCTCTGA GAAAGTCTTT GTCTCACTTC CTACTGAACT GGAGGATTTG ATACCTGAAG 
     2701 TAGAAGAATT TTACAAAAAA AATCATAGTG GTAGAAAATT ACATTGGCAC CATCTCATGT 
     2761 CAAATGGAAT TATAACATTT AAAAACGAAG TAGGTCAGTA CGATTTGGAA GTAACCACGT 
     2821 TTCAGTTGGC TGTGTTGTTT GCATGGAACC AAAGACCTAG AGAGAAAATC AGCTTTGAGA 
     2881 ATCTAAAACT TGCAACGGAA CTCCCTGATG CTGAACTTAG AAGGACTTTA TGGTCTTTAG 
     2941 TAGCTTTTCC CAAGCTCAAA CGGCAAGTTT TATTGTACGA CCCTCAAGTC AATTCACCCA 
     3001 AAGATTTTAC AGAAGGCACC CTCTTCTCAG TCAACCAGGA CTTTAGTTTA ATAAAAAATG 
     3061 CAAAAGTACA GAAAAGGGGA AAAATCAATT TGATTGGACG CTTGCAGCTC ACTACAGAAC 
     3121 GGATGAGAGA AGAAGAAAAT GAAGGAATAG TTCAACTAAG AATATTAAGA ACCCAGGAAG 
     3181 CCATCATACA AATAATGAAA ATGAGGAAGA AAATTAGCAA TGCTCAGCTG CAGACTGAAT 
     3241 TAGTAGAAAT TTTGAAAAAC ATGTTCTTAC CACAGAAGAA AATGATAAAA GAGCAAATCG 
     3301 AATGGCTGAT CGAGCACAAG TACATCAGGA GGGATGAGGC CGACATCAAC ACCTTCATAT 
     3361 ACATGGCGAG CGGACCGACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     3421 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     3481 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     3541 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     3601 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     3661 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     3721 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     3781 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     3841 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     3901 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     3961 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     4021 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     4081 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     4141 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     4201 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     4261 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     4321 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     4381 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     4441 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     4501 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     4561 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     4621 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     4681 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     4741 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     4801 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     4861 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     4921 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     4981 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     5041 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     5101 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     5161 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     5221 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     5281 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     5341 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     5401 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     5461 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     5521 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     5581 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     5641 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     5701 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     5761 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     5821 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     5881 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     5941 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     6001 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     6061 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     6121 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     6181 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     6241 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     6301 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     6361 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     6421 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     6481 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     6541 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6601 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     6661 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     6721 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     6781 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     6841 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     6901 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     6961 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     7021 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     7081 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     7141 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     7201 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//