LOCUS       RR202985                7539 bp    DNA     circular  SYN 
DEFINITION  Cdh1 (Myc-DDK-tagged ORF) - Rat cadherin 1 (Cdh1), (10 ug)
ACCESSION   NM_031334
VERSION     NM_031334.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3696..3787 
                     /label="Myc-DDK"  
     misc_feature    3817..4402 
                     /label="PolyA signal"  
     misc_feature    3874..3893 
                     /label="Restriction Enzyme"  
     misc_feature    4678..5350 
                     /label="ColE1"  
     misc_feature    5780..6574 
                     /label="Kan/Neo"  
     misc_feature    6609..6911 
                     /label="SV40 ori"  
     misc_feature    6997..7474 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3678..3684 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3678 
                     /label="Cdh1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGAGCCCGG TGCCGCAGCT TCTCCGCGCT CCTGCTCCTG CTGCAGGTCT 
     1081 CCTCGTGGCT TTGTCAGCAG CCGGAGTCGG AGTCTGACTC CTGCCGTCCC GGCTTCAGTT 
     1141 CCGAGGTCTA CACCTTCCTG GTGCCGGAGA GGCACCTGGA GAGAGGCCAC ATCCTGGGCA 
     1201 GAGTGAAATT TGAAGGATGC ACCGGCCGTC CAAGGACAGC CTTCTTTTCT GAAGACTCCC 
     1261 GATTCAAAGT GTCTACAGAT GGCGTCATCA CAGTCAAACG GCATCTAAAG CTTCACAAGC 
     1321 TGGAGACCAG TTTTCTCGTC CATGCCTGGG ACTCCAGTTA CAGGAAGCTT TCTACCAAAG 
     1381 TGACACTGAA GTCCCTGGGC CACCACCACC ACCGGCATCA CCACAGAGAC CCTGTCTCTG 
     1441 AATCCAACCC AGAGCTGCTC ACGTTTCCCA GCTTTCACCA GGGTCTGAGA AGACAGAAAC 
     1501 GAGACTGGGT CATCCCTCCC ATCAACTGCC CGGAAAATCA AAAGGGCGAA TTCCCCCAGC 
     1561 GACTGGTTCA GATCAAATCC AACAGGGACA AAGAGACAAC GGTTTTCTAC AGCATCACCG 
     1621 GCCCAGGAGC TGACAAACCC CCTGTTGGCG TTTTCATCAT TGAGAGGGAG ACAGGCTGGC 
     1681 TGAAAGTGAC GCAGCCTCTG GACAGAGAAG CCATTGACAA GTACCTTCTC TACTCTCATG 
     1741 CTGTGTCATC AAATGGGGAA GCCGTGGAGG ATCCCATGGA GATAGTGGTC ACAGTCACAG 
     1801 ATCAGAATGA CAACAGGCCA GAGTTTATCC AGGAGGTCTT TGAGGGATCT GTTGCAGAAG 
     1861 GCGCTCTTCC AGGAACCTCC GTGATGCAGG TCTCAGCCAC TGATGCAGAC GATGACATAA 
     1921 ACACCTACAA TGCTGCCATC GCCTACACCA TCCTCAGCCA AGATCCTGAG CTGCCTCACA 
     1981 AAAACATGTT CACTGTCAAC CGGGACACTG GGGTCATCAG TGTGGTCACC TCCGGACTGG 
     2041 ACCGAGAGAG TTACCCTACA TATACTCTGG TGGTTCAGGC TGCCGACCTT CAAGGCGAAG 
     2101 GCCTAAGCAC AACAGCAAAA GCTGTGATCA CTGTCAAGGA TATTAATGAC AACGCTCCCA 
     2161 TCTTCAACCC AAGCACGTAC CAGGGTCAAG TGCTTGAGAA TGAGGTCGGT GCCCGTATTG 
     2221 CCACACTCAA GGTGACTGAT GATGATGCCC CCAACACTCC AGCGTGGAAT GCTGTGTACA 
     2281 CCGTAGTCAA TGATCCTGAT CATCAGTTCA CTGTCATCAC AGACCCCAAG ACCAACGAGG 
     2341 GCATTCTGAA AACAGCCAAG GGCTTGGATT TTGAGGCCAA GCAGCAGTAC ATTCTGCACG 
     2401 TGACAGTGGA AAATGAGGAG CCCTTTGAGG GGTCTCTTGT CCCTTCCACA GCCACTGTCA 
     2461 CCGTGGATGT GGTAGACGTG AATGAAGCCC CCATTTTTGT GCCTGCGGAG AAGAGAGTCG 
     2521 AGGTGCCTGA GGACTTTGGT GTGGGTCTGG AGATCGCATC TTACACTGCG CGAGAGCCAG 
     2581 ACACATTCAT GGAACAGAAG ATCACGTATC GGATTTGGAG GGACACTGCC AATTGGCTGG 
     2641 AGATTAACCC AGAGACTGGG GTCATTTCCA CTCGGGCTGA GATGGACAGA GAAGATTCGG 
     2701 AGCATGTGAA GAACAGCACG TATACAGCTC TCATCATTGC CACAGATGAT GGTTCACCCA 
     2761 TTGCCACTGG CACAGGGACT CTTCTCCTGG TCCTGTCAGA CGTCAACGAC AATGCTCCCA 
     2821 TCCCAGAACC TCGAAATATG CAGTTCTGCC AGAGAAACCC GAAGCCCCAT GTCATCACCA 
     2881 TCTTGGATCC AGACCTTCCC CCAAACACAT CCCCCTTCAC TGCAGAGCTC ACCCATGGGG 
     2941 CCAGCGTCAA CTGGACCATT GAGTACAATG ACGCAGAACA AGAATCTCTC ATTTTGCAAC 
     3001 CAAGAAAGGA CTTAGAGATT GGCGAATACA AAATCAATCT CAAGCTCTCG GATAACCAGA 
     3061 ATAAAGACCA GGTGACCACG TTGGAGGTCC ACGTGTGTGA CTGTGAAGGG ACCGTCAACA 
     3121 ACTGCATGAA GGCGATCTCC CTGGAAGCAG GATTACAAGT TCCCGCCATC CTTGGAATCC 
     3181 TGGGAGGGAT CCTGGCCCTC CTGATTCTGA TCCTCCTGCT CCTACTGTTT CTACGGAGGA 
     3241 GAACGGTGGT CAAAGAGCCC TTGCTGCCAC CAGATGACGA TACCCGGGAC AATGTGTATT 
     3301 ACTATGATGA AGAGGGAGGT GGAGAAGAAG ACCAGGACTT TGATTTGAGC CAGCTGCACA 
     3361 GGGGCCTTGA TGCCAGACCG GAAGTGATTC GAAATGATGT GGCTCCCACC CTCATGAGCA 
     3421 TGCCCCAGTA TCGTCCCCGT CCAGCCAATC CTGATGAAAT CGGGAACTTC ATCGATGAAA 
     3481 ACCTGAAGGC AGCGGACAGT GACCCCACAG CGCCCCCTTA CGACTCTCTG TTGGTGTTTG 
     3541 ACTATGAGGG GAGTGGTTCT GAAGCTGCCT CCCTGAGCTC GCTGAACTCC TCTGAGTCAG 
     3601 ATCAGGACCA GGACTACGAT TATCTGAACG AATGGGGCAA CCGGTTCAAG AAGCTGGCCG 
     3661 ATATGTATGG TGGCGGCGAA GAAGACACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     3721 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     3781 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     3841 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     3901 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     3961 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4021 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4081 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4141 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4201 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     4261 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     4321 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     4381 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     4441 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     4501 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     4561 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     4621 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     4681 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     4741 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     4801 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     4861 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     4921 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     4981 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5041 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     5101 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     5161 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     5221 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     5281 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     5341 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     5401 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     5461 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     5521 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     5581 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     5641 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     5701 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     5761 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     5821 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     5881 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     5941 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6001 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6061 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6121 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     6181 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     6241 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     6301 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     6361 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     6421 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     6481 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     6541 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     6601 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     6661 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     6721 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     6781 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     6841 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     6901 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     6961 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7021 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7081 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7141 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     7201 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     7261 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     7321 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     7381 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     7441 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     7501 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//