LOCUS       RR202970                7128 bp    DNA     circular  SYN 
DEFINITION  Spire1 (Myc-DDK-tagged ORF) - Rat spire homolog 1 (Drosophila) (Spire1), (10 ug)
ACCESSION   NM_001107381
VERSION     NM_001107381.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3285..3376 
                     /label="Myc-DDK"  
     misc_feature    3406..3991 
                     /label="PolyA signal"  
     misc_feature    3463..3482 
                     /label="Restriction Enzyme"  
     misc_feature    4267..4939 
                     /label="ColE1"  
     misc_feature    5369..6163 
                     /label="Kan/Neo"  
     misc_feature    6198..6500 
                     /label="SV40 ori"  
     misc_feature    6586..7063 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3267..3273 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3267 
                     /label="Spire1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGCAGCCC TCAAGCCCGG GCGGCGAGGG GCCGCAGCTC GGCGCGACAG 
     1081 GGGACCCGCG CGACGCGCTG AGCTTGGAAG AGATCCTGCG GCTCTACAAC CAGCCCATCA 
     1141 ACGAGGAGCA GGCGTGGGCC GTGTGCTTCC AGTGCTGCGG CTCCCTGCGC GCCGCCGCCC 
     1201 GCCGCCAGCC CCACCGCCGC GTGCGCTCGG CCGCGCAGAT CCGCGTTATC GAATCCCTGG 
     1261 GAATTATTAT TTATAAAGCA CTGGACTATG GCTTGAAGGA AAATGAAGAG AGGGAGCTGA 
     1321 GCCCTCCTCT CGAGCAGCTC ATCGACCAAA TGGCCAATAC CTCGGAGGCG GATGGAAGCA 
     1381 ATGATGAAGG GTACGAGGCA GCAGATGAAG GCCCAGACGA GGAAGATGGA GAGAGGAGAA 
     1441 GCAGCGCAGC CATTCGGTCC TATCAAGATG TCATGAAGAT ATGTGCTGCT CATCTCCCCA 
     1501 CTGAGTCGGA GGCACCGAAT CATTATCAGG CAGTATGTCG GGCACTGTTT GCAGAAACCA 
     1561 TGGAACTGCA TACGTTTCTG ACCAAAATTA AGAGTGCGAA GGAGAACCTT AAGAAGATTC 
     1621 AAGAAATGGA AAAGAGTGAT GACTGTAGCA CAGATCTGGA GGACCTGAAA AATGCAGACT 
     1681 GGGCACGGTT CTGGGTACAG GTGATGAGGG ATTTGCGAAA TGGGGTAAAA CTTAAGAAAG 
     1741 TCCAACAGCG GCAGTACAAC CCATTGCCCA TTGAATACCA GCTCACCCCG TATGAGATGC 
     1801 TCATGGACGA CATTCGGTGC AAAAGATACA CCTTGAGAAA AGTAATGGTA AATGGTGACA 
     1861 TTCCCCCTCG GTTAAGAAAG AGTGCTCATG AGATCATCCT TGACTTCATC AGATCAAGAC 
     1921 CCCCTTTAAA TCCAGCTTCA GCCAGAAAAC TGAAACCAAC CCCACCACGG CCACGGAGCC 
     1981 TCCATGAAAG AATACTAGAA GAAATTAAAG CAGAAAGAAA GCTTCGGCCT GTCTCACCAG 
     2041 AAGAAATTAG ACGGAGCAGA CTAGATGTTA CTACACCAGA ATCTCCAAAG AATGTGGGGA 
     2101 AATCATCTGT GGTGAATGGA GACTTAACAT CTCAAACGAA AGAAAATGGG CTGGGTGCTG 
     2161 CCCAGCCGGG GCCTGCACAG CCAAAGAGGC TCCTCAAAGC CCCGACGCTG GCTGAGCTGG 
     2221 ACAGCTCTGA CTCTGAGGAG GAAACTCTGC ACAAGTCAAC CAGCAGCAGC AGTGCATCTC 
     2281 CCTCCCTCTA TGAGGACCCA GTACTGGAGG CCATGTGCTC CAAGAAGAAG CCTCCAAAAT 
     2341 TCTTGCCCAT CTCATCAACA CCCCAGCCCG AGAGAAGGCA ACCACCACAG AGGCGCCACT 
     2401 CCATTGAGAA GGAAACTCCT ACAAATGTGA GGCAGTTTCT GCCCCCATCC AGGCAGAGCT 
     2461 CACGTTCTCT TGTTCCTAGG ATAACCAGTG TATGGCCAAG GACGCCTTTC CGACCACTTT 
     2521 TCTCTGCCAT ACAAACAGCT TCTCTGCTGA GCTCTCATCC TTTTGAAGCA GCCATGTTTG 
     2581 GGGTAGCAGG GGCTATGTAT TATCTGTTTG AGAGAGCTTT TACCAGCAGG TGGAAACCCT 
     2641 CAAAGGAGGA ATTCTGCTAC CCAGTGGAAT GCCTCGCTCT CACTGTGGAG GAAGTGATGC 
     2701 ACATTCGTCA GGTCCTGGTG AAAGCGGAGC TGGAAAAGTA CCAACAGTAT AAGGATGTCT 
     2761 ACACTGCCCT GAAAAAAGGA AAGCTCTGCT TCTGTTGCCG AACCCGGAGG TTTTCCTTCT 
     2821 TCACCTGGTC CTACACCTGT CAGTTCTGTA AGAGGCCCGT GTGCTCACAG TGTTGCAAAA 
     2881 AGATGCGGCT GCCTTCGAAG CCATACTCCA CTTTACCTAT CTTCTCTTTG GGACCTTCTG 
     2941 CCTTGCAAAG AGGGGAAAGT TGTTCGAGGT CAGAAAAACC TTCTACTGGT CACCATCGGC 
     3001 CGCTTCGAAG TATTACCAGA TTCTCTTCAA AATCAAAGTC TGTGGACAAA TCAGATGAAG 
     3061 AACTCCAGTT TCCCAAAGAG TTCATGGAAG ATTGGAGCAC AATGGAGGTG TGTGTAGACT 
     3121 GCAAAAAGTT CATTTCAGAG ATCATCAGCT CAAGCCGGCG CAGCCTGGTG TTGGCCAACA 
     3181 AAAGAGCCAG GTTGAAAAGG AAGACACAGT CTTTCTACAT GTCCTCAGCG GGCCCCTCGG 
     3241 AGTACTGCCC TTCAGAACGG ACTATCAACG AGATCACGCG TACGCGGCCG CTCGAGCAGA 
     3301 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     3361 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     3421 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     3481 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     3541 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     3601 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     3661 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     3721 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     3781 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     3841 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     3901 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     3961 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4021 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     4081 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     4141 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     4201 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     4261 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     4321 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     4381 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     4441 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     4501 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     4561 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     4621 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     4681 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     4741 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     4801 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     4861 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     4921 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     4981 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     5041 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     5101 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     5161 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     5221 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     5281 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     5341 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     5401 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     5461 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     5521 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     5581 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     5641 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     5701 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     5761 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     5821 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     5881 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     5941 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     6001 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     6061 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     6121 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     6181 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     6241 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     6301 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     6361 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6421 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     6481 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     6541 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     6601 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     6661 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     6721 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     6781 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     6841 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     6901 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     6961 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     7021 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     7081 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//