LOCUS       RR202933                7620 bp    DNA     circular  SYN 
DEFINITION  Gtf2i (Myc-DDK-tagged ORF) - Rat general transcription factor II I (Gtf2i), (10 ug)
ACCESSION   NM_001001512
VERSION     NM_001001512.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3777..3868 
                     /label="Myc-DDK"  
     misc_feature    3898..4483 
                     /label="PolyA signal"  
     misc_feature    3955..3974 
                     /label="Restriction Enzyme"  
     misc_feature    4759..5431 
                     /label="ColE1"  
     misc_feature    5861..6655 
                     /label="Kan/Neo"  
     misc_feature    6690..6992 
                     /label="SV40 ori"  
     misc_feature    7078..7555 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3759..3765 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3759 
                     /label="Gtf2i"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCAAGTA GCGATGTCCG CGCTGCCTGC GGAGGATGAG GAGTCTTCGG 
     1081 AGAGCAGGAT GGTGGTGACG TTCCTCATGT CTGCCCTGGA GTCCATGTGT AAAGAGCTGG 
     1141 CCAAGTCCAA GGCTGAGGTG GCCTGCATCG CCGTGTATGA GACAGATGTG TTTGTCGTTG 
     1201 GAACCGAAAG AGGGCGTGCT TTTGTCAATA CCAGAAAAGA TTTCCAAAAA GATTTTGTGA 
     1261 AGTATTGTGT TGAAGAAGAA GAAAAGGCCG CCGAGATGCA TAAGATGAAA TGTACAACCC 
     1321 AGGCCAACCG GATGAGCGTG GATGCTGTAG AAATTGAGAC CCTCAGAAAA ACGGTGGAGG 
     1381 ACTATTTCTG CTTTTGTTAT GGGAAAGCCT TAGGCAAATC CACAGTGGTC CCTGTGCCAT 
     1441 ATGAGAAGAT GCTGCGTGAC CAGTCAGCTG TGGCGGTGCA GGGGCTTCCC GAGGGGGTCG 
     1501 CCTTCAAGCA TCCCGAGCAC TATGACCTCG CAACCTTGAA GTGGATTCTG GAGAACAAGG 
     1561 CGGGGATTTC CTTCATCATC AAGAGACCTT TCCTCGAGCC GAAGAAACAC CTTGGTGGTC 
     1621 GAGTGATGGC GGCCGATGCT GACAGGCCCA TGCTGTCTCC GGGTGGAAGT TGTGGCCCCA 
     1681 TCAAAGTGAA AACTGAGCCC ACGGAAGATT CTGATGATGA TTATTCTCCA CCTACCAAGA 
     1741 GGCCAAAGAG CAGTGAGCCG CCCCCACCCC CACCGGTCCC AGAGCCCACC AACGCCGGCA 
     1801 AGCGCAAAGT GAGGGAGTTC AACTTTGAGA AATGGAACGC GCGCATCACT GACCTACGGA 
     1861 AACAAGTGGA AGAATTGTTT GAAAGAAAAT ACGCTCAAGC TATAAAAGCC AAAGGCCCGG 
     1921 TGACGATCCC ATACCCTCTT TTCCAGTCGC ACGTCGAAGA TCTGTATGTA GAGGGCCTTC 
     1981 CTGAAGGGAT CCCCTTTAGA AGGCCATCAA CGTACGGCAT TCCTCGCCTG GAGAGGATAT 
     2041 TGCTGGCAAA GGAGAGGATC CGCTTTGTGA TCAAGAAACA TGAGCTTCTG AACTCAACAC 
     2101 GAGAAGATTT ACAGCTGGAC AAGCCCGCAT CAGGAGTGAA GGAAGAGTGG TATGCTCGTA 
     2161 TCACCAAACT GAGGAAGATG GTTGACCAGC TCTTCTGCAA GAAATTTGCT GAGGCCTTGG 
     2221 GAAGCACAGA AGCCAAGGCT GTACCGTACC AAAAATTCGA GGCCTATCCC AATGATCTCT 
     2281 ATGTGGAAGG ACTTCCAGAA AACATTCCTT TCCGAAGCCC CTCATGGTAT GGAATCCCAA 
     2341 GACTGGAAAA AATCATCCAA GTGGGCAATC GAATTAAATT TGTTATCAAG AGACCAGAAC 
     2401 TGCTGACTCA CAGTACAACT GAGGTGACTC AGCCACGGAC GAATACGCCA GTCAAGGAAG 
     2461 ATTGGAACGT CAGGATCACC AAGCTCCGCA AGCAGGTGGA GGAGATCTTT AACTTGAAGT 
     2521 TTGCTCAAGC TCTTGGCCTC ACAGAGGCTG TGAAGGTGCC GTATCCTGTG TTCGAATCCA 
     2581 ACCCAGAATT TCTGTATGTG GAAGGGCTCC CTGAGGGGAT CCCCTTCCGA AGCCCTACCT 
     2641 GGTTTGGGAT TCCACGCCTT GAGAGGATTG TCCGTGGGAG CAATAAGATC AAGTTTGTTG 
     2701 TTAAAAAGCC CGAGCTAGTT GTGTCCTACT TGCCTCCTGG GATGGCTAGC AAAATCAACA 
     2761 CTAAAGCATT GCAGTCCCCG AAACGACCAC GAAGCCCTGG GAGCAACTCC AAGGTCCCTG 
     2821 AAATCGAGGT CACTGTGGAA GGCCCCAACA ACAACAGTCC TCAGACCTCT ACCGTTCGAA 
     2881 CTCCTACCCA GACCAACGGT TCAAACGTTC CCTTCAAGCC TCGAGGGAGA GAGTTTTCCT 
     2941 TTGAGGCCTG GAACGCTAAG ATCACAGACC TGAAGCAGAA AGTGGAGAAT CTCTTCAATG 
     3001 AAAAGTGCGG GGAAGCTCTT GGCCTTAAGC AGGCTGTGAA AGTACCGTTT GCGCTATTTG 
     3061 AGTCCTTCCC AGAGGACTTC TACGTAGAAG GCTTACCCGA GGGCGTGCCC TTCCGAAGAC 
     3121 CATCCACTTT TGGCATTCCA AGGCTGGAGA AGATCCTCCG GAACAAGGCC AAGATAAAGT 
     3181 TCATCATTAA AAAACCTGAG ATGTTCGAGA CGGCCATCAA AGAGAGCACC TCTTCCAAAA 
     3241 GCCCTCCGAG GAAGACGAAC TCATCCCCCA GTGTCAATAC GACAGCCTCA GGTGTGGAAG 
     3301 ATCTTAACAT CATTCAGGTG ACAATCCCAG ATGACGACAA CGAACGACTC TCCAAAGTCG 
     3361 AGAAGGCCAG GCAGCTTCGG GAGCAAGTCA ACGACCTCTT CAGCCGAAAG TTTGGTGAAG 
     3421 CTATTGGGAT GGGCTTCCCC GTGAAGGTCC CCTACAGGAA AATCACCATC AACCCTGGCT 
     3481 GCGTGGTGGT CGATGGCATG CCCCCGGGGG TGTCCTTCAA AGCCCCCAGC TATCTGGAGA 
     3541 TCAGCTCCAT GAGAAGGATC TTAGATTCTG CTGAGTTTAT CAAGTTCACT GTCATTAGAC 
     3601 CATTCCCAGG ACTGGTGATT AATAACCAGC TGGTTGATCA GAATGAGTCT GAAGGCCCTG 
     3661 TGATACAAGA ATCAGCTGAG GCAAGCCAGT TGGAAGTTCC AGCAACAGAA GAAATCAAAG 
     3721 AGACAGATGG GAGCTCACAG ATCAAGCAGG AGCCAGACCC CACGTGGACG CGTACGCGGC 
     3781 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     3841 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     3901 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     3961 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4021 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4081 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4141 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4201 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4261 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4321 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4381 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4441 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     4501 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     4561 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     4621 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     4681 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     4741 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     4801 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     4861 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     4921 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     4981 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5041 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5101 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5161 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5221 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     5281 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     5341 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     5401 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     5461 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     5521 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     5581 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     5641 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     5701 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     5761 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     5821 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     5881 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     5941 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6001 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6061 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6121 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6181 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6241 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     6301 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     6361 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     6421 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     6481 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     6541 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     6601 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     6661 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     6721 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     6781 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     6841 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     6901 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     6961 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7021 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7081 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7141 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7201 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7261 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     7321 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     7381 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     7441 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     7501 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     7561 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//