LOCUS       RR202843                8892 bp    DNA     circular  SYN 
DEFINITION  Pard3 (Myc-DDK-tagged ORF) - Rat par-3 (partitioning defective 3) homolog (C. elegans) (Pard3), (10 ug)
ACCESSION   NM_031235
VERSION     NM_031235.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5049..5140 
                     /label="Myc-DDK"  
     misc_feature    5170..5755 
                     /label="PolyA signal"  
     misc_feature    5227..5246 
                     /label="Restriction Enzyme"  
     misc_feature    6031..6703 
                     /label="ColE1"  
     misc_feature    7133..7927 
                     /label="Kan/Neo"  
     misc_feature    7962..8264 
                     /label="SV40 ori"  
     misc_feature    8350..8827 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5031..5037 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5031 
                     /label="Pard3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAAGTGACC GTGTGCTTCG GGAGGACCCG GGTGGTCGTG CCGTGCGGAG 
     1081 ATGGCCGCAT GAAAGTTTTC AGCCTTATCC AGCAGGCGGT GACCCGCTAC CGGAAGGCCG 
     1141 TGGCCAAGGA TCCAAACTAC TGGATACAGG TACATCGCTT GGAGCATGGG GACGGTGGGA 
     1201 TTCTAGACCT GGATGACATC CTCTGTGACG TTGCCGATGA CAAAGACAGA CTGGTAGCAG 
     1261 TGTTTGATGA GCAGGATCCC CACCATGGAG GAGATGGTAC GAGTGCCAGC TCCACGGGAA 
     1321 CCCAGAGTCC AGAGATATTC GGCAGTGAGC TGGGCACCAA CAATGTTTCT GCTTTTCGGC 
     1381 CTTATCAAAC CACAAGTGAA ATTGAGGTCA CGCCTTCAGT CCTTCGGGCA AATATGCCTC 
     1441 TTCACGTCCG ACGGAGCAGT GACCCAGCTT TAACTGGCCT ATCCACTTCC GTCAGTGATA 
     1501 ACAACTTTTC CTCAGAGGAG CCCTCCAGGA AAAACCCCAC CCGCTGGTCC ACGACAGCTG 
     1561 GCTTTCTCAA GCAGAACACC ACTGGAAGTC CCAAAACCTG CGACAGGAAG AAAGATGAAA 
     1621 ACTACAGGAG CCTCCCACGG GATCCCAGTA GCTGGTCCAA CCAGTTCCAG AGAGACAACG 
     1681 CCCGCTCCTC TCTGAGTGCC AGCCACCCAA TGGTAGACCG GTGGCTGGAG AAGCAAGAAC 
     1741 AGGACGAGGA AGGCACAGAA GAAGACAGCA GCCGAGTGGA GCCGGTTGGA CATGCTGATA 
     1801 CTGGACTGGA GAATATGCCC AACTTTTCCC TCGATGATAT GGTAAAGCTC GTACAAGTCC 
     1861 CCAACGATGG AGGGCCCCTG GGAATCCATG TAGTGCCTTT CAGTGCTCGA GGCGGCAGAA 
     1921 CACTGGGGTT GTTAGTGAAG CGGTTGGAGA AAGGTGGGAA GGCTGAACAA GAAAACCTTT 
     1981 TCCACGAGAA TGACTGCATT GTGAGGATTA ACGATGGAGA TCTTCGAAAC AGAAGATTTG 
     2041 AGCAAGCACA GCATATGTTC CGCCAAGCCA TGCGTGCGCG AGTCATTTGG TTCCACGTGG 
     2101 TCCCTGCAGC GAACAAGGAG CAGTATGAAC AGCTCTCCCA ACGAGAGATG AACAACTATT 
     2161 CCCCTGGCCG CTTCAGCCCC GACAGCCACT GTGTGGCCAA CAGGAGTGTG GCCAACAATG 
     2221 CGCCTCAGGC GTTGCCCAGA GCACCCAGAC TGAGCCAGCC ACCTGAGCAG CTGGATGCTC 
     2281 ACCCCCGACT ACCTCATAGT GCTCATGCCT CAACCAAACC GCCCACAGCC CCAGCCTTGG 
     2341 CTCCACCGAA TGTGCTTAGT ACCAGTGTCG GCAGTGTGTA CAACACGAAG AGAGTAGGCA 
     2401 AGAGGCTCAA CATTCAGCTT AAAAAAGGTA CAGAAGGACT GGGATTTAGC ATCACTTCCC 
     2461 GAGACGTAAC TATAGGTGGC TCAGCTCCCA TTTATGTCAA GAACATTCTT CCTCGAGGGG 
     2521 CTGCCATTCA GGATGGCAGG CTCAAGGCGG GAGACCGGCT AATAGAGGTC AATGGAGTAG 
     2581 ATTTAGCGGG CAAGTCCCAG GAGGAAGTTG TTTCCCTGTT GAGAAGCACC AAGATGGAGG 
     2641 GGACCGTGAG CCTTCTGGTC TTTCGTCAAG AAGAGGCTTT CCACCCAAGG GAAATGAATG 
     2701 CCGAACCCAG CCAGATGCAG AGTCCAAAAG AAACGAAAGC CGAAGACGAG GACATTGTTC 
     2761 TCACACCTGA CGGTACCAGG GAGTTTCTGA CTTTCGAAGT TCCACTGAAT GACTCAGGGT 
     2821 CTGCAGGGCT TGGTGTCAGC GTCAAGGGGA ACCGCTCCAA AGAGAACCAT GCAGATTTGG 
     2881 GGATCTTCGT TAAGTCCATC ATTAACGGGG GAGCTGCATC TAAAGATGGG AGGCTGAGGG 
     2941 TAAATGACCA GCTGATAGCT GTGAACGGAG AGTCTCTGCT GGGCAAAGCC AACCAGGAAG 
     3001 CCATGGAGAC TCTGAGGAGG TCCATGTCCA CCGAGGGCAA CAAGCGTGGC ATGATCCAGC 
     3061 TCATCGTGGC GAGGCGGATC AGCAGATGTA ACGAGTTGCG GTCTCCTGGG AGCCCCGCTG 
     3121 CACCCGAGCT GCCCATAGAA ACAGAGTTGG ATGACAGAGA ACGCAGGATC TCACACTCCC 
     3181 TCTACAGTGG GATCGAGGGG CTGGATGAGT CTCCCACCAG GAATGCCGCA CTCAGCAGGA 
     3241 TAATGGGTGA GTCAGGTAAA TGCCAGCTCT CCCCAACCGT GAACATGCCA CATGATGACA 
     3301 CTGTCATGAT TGAAGATGAC AGGCTGCCTG TGCTCCCTCC TCACCTCTCT GACCAGTCCT 
     3361 CCTCCAGCTC CCATGATGAC GTGGGATTCA TAATGACAGA AGCAGGCACG TGGGCCAAGG 
     3421 CTACCATCAG TGACTCAGCC GACTGCTCAT TGAGTCCAGA TGTTGATCCG GTTCTCGCTT 
     3481 TTCAACGAGA AGGATTTGGA CGCCAGAGTA TGTCAGAAAA ACGCACAAAG CAATTTTCAA 
     3541 ATGCCAGTCA ATTGGATTTC GTTAAAACAC GAAAATCAAA AAGCATGGAT TTAGGTATAG 
     3601 CTGACGAGAC TAAACTCAAT ACAGTGGATG ACCAGAGAGC AGGCTCCCCC AATAGAGATG 
     3661 TGGGACCCTC CTTGGGTCTG AAGAAATCCA GCTCTTTAGA AAGTCTGCAG ACGGCTGTTG 
     3721 CTGAGGTGAC CCTGAATGGG AACATTCCTT TCCACCGCCC ACGGCCACGA ATCATCCGAG 
     3781 GAAGGGGCTG CAACGAGAGC TTCAGAGCCG CCATTGACAA GTCCTACGAT AAGCCCATGG 
     3841 TGGATGACGA CGACGAAGGC ATGGAGACCT TGGAAGAAGA CACAGAAGAA AGTTCAAGGT 
     3901 CAGGGAGGGA GTCCGTGTCC ACGTCCAGTG ATCAGCCTTC CTATTCTCTG GAGAGACAAA 
     3961 TGAATGGAGA CCCAGAGAAG AGGGACAAGG CAGAGAAGAA AAAGGACAAA GCCGGAAAGG 
     4021 ATAAGAAGAA AGACCGAGAG AAGGAGAAGG ATAAACTGAA AGCCAAGAAG GGGATGCTGA 
     4081 AAGGCTTGGG GGACATGTTC AGGTTTGGCA AACATCGAAA AGATGACAAG ATGGAAAAAA 
     4141 TGGGTCGAAT AAAAATCCAG GACTCTTTCA CCTCAGAAGA GGACAGGGTG CGGATGAAGG 
     4201 AGGAACAGGA GAGGATTCAA GCCAAAACTC GAGAATTTAG GGAGCGGCAA GCTCGAGAGC 
     4261 GCGACTATGC TGAAATCCAA GATTTCCATC GAACATTTGG CTGTGATGAC GAGTTGCTGT 
     4321 ATGGGGGCAT GTCATCCTAT GATGGCTGCC TGGCTCTCAA TGCCAGACCC CAGAGCCCAA 
     4381 GAGAAGGTCA CCTGATGGAC ACTTTGTATG CACAAGTAAA GAAACCTCGG AGCTCCAAAC 
     4441 CTGGGGACAG CAATCGATCC ACCCCTAGCA ACCATGACCG GATACAACGT CTACGGCAAG 
     4501 AATTCCAGCA AGCCAAACAG GATGAGGACG TGGAAGACCG GCGCCGTACC TACAGCTTTG 
     4561 AGCAATCTTG GTCCAGCTCA CGGCCAGCAT CGCAGAGTGG TCGGCACTCG GTGTCAGTGG 
     4621 AGGTTCAAGT GCAGCGGCAG CGCCAGGAGG AGCGAGAGAG CTTCCAGCAG GCCCAGCGCC 
     4681 AGTACAGCTC CCTGCCAAGA CAAAGCAGGA AGAATGCCAG CTCTGTATCA CAGGATTCCT 
     4741 GGGAACAGAA CTACGCCCCT GGTGAAGGCT TCCAGAGTGC CAAGGAGAAC CCCAGGTATT 
     4801 CCAGTTACCA GGGCTCCAGG AACGGCTATC TAGGTGGCCA TGGCTTTAAT GCCAGGGTCA 
     4861 TGCTGGAGAC CCAGGAGCTT CTCCGACAGG AGCAGAGGCG AAAAGAGCAG CAACTGAAGA 
     4921 AGCAGCCACC AGCGGATGGA GTCAGAGGGC CCTTCCGGCA AGATGTACCC CCGTCTCCAT 
     4981 CTCAGGTGGC CAGGCTGAAC AGACTGCAGA CACCCGAGAA AGGGCGGCCC TTCTACTCCA 
     5041 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     5101 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     5161 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     5221 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     5281 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     5341 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     5401 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     5461 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     5521 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     5581 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     5641 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5701 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5761 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     5821 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5881 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5941 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     6001 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     6061 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     6121 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     6181 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     6241 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     6301 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     6361 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     6421 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     6481 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     6541 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     6601 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     6661 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     6721 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     6781 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6841 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6901 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6961 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     7021 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     7081 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     7141 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     7201 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     7261 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     7321 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     7381 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     7441 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     7501 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     7561 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     7621 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     7681 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     7741 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     7801 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7861 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7921 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7981 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     8041 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     8101 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     8161 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     8221 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8281 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     8341 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     8401 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     8461 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     8521 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     8581 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     8641 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     8701 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     8761 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     8821 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8881 AACGCGAATT TT
//