LOCUS       RR202827                6900 bp    DNA     circular  SYN 
DEFINITION  Vasn (Myc-DDK-tagged ORF) - Rat vasorin (Vasn), (10 ug)
ACCESSION   NM_001109382
VERSION     NM_001109382.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3057..3148 
                     /label="Myc-DDK"  
     misc_feature    3178..3763 
                     /label="PolyA signal"  
     misc_feature    3235..3254 
                     /label="Restriction Enzyme"  
     misc_feature    4039..4711 
                     /label="ColE1"  
     misc_feature    5141..5935 
                     /label="Kan/Neo"  
     misc_feature    5970..6272 
                     /label="SV40 ori"  
     misc_feature    6358..6835 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3039..3045 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3039 
                     /label="Vasn"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCACTCCAGG AGCTGCCTGC CACCTCTTCT GTTGTTGCTC CTGGTGCTCC 
     1081 TGGGGTCTGG AGTACAGAGC TGCCCATCAG GCTGCCAGTG CAACCAACCA CAGACAGTCT 
     1141 TCTGCACTGC CCGTCAGGGA ACCACGGTGC CCCGAGACGT GCCGCCTGAC ACAGTGGGCC 
     1201 TGTACATCTT TGAGAACGGC ATCACTACAC TTGATGTAGG CTGTTTTGCT GGCTTCCCAG 
     1261 GCCTGCAGCT TCTGGACTTG TCACAGAACC AGATCACTAG CCTGCCCGGT GGCATCTTTC 
     1321 AGCCACTTGT GAACCTCAGT AACCTGGACC TGACTGCTAA CAAACTGCAC GAGATCTCCA 
     1381 ACGAGACCTT CCGTGGCCTG CGGCGCCTCG AACGCCTCTA CCTGGGCAAG AACCGCATTC 
     1441 GCCACATCCA GCCTGGTGCC TTCGATGCAC TTGACCACCT CCTGGAGCTC AAGCTGCCAG 
     1501 ACAATGAGCT TCGGGTGCTG CCCCCACTGC ACTTGCCTCG CCTGCTGCTG CTTGACCTCA 
     1561 GCCACAACAG TATCCCAGCC CTGGAAGCTG GAATACTGGA TACTGCCAAT GTGGAGGCAC 
     1621 TGCGGCTGGC TGGCCTCGGG CTGCGGCAGC TGGATGAGGG GCTTTTTGGC CGCCTTCGCA 
     1681 ACCTCCATGA CCTGGATGTT TCTGACAACC AGTTGGGGCA CATGCCCTCC GTGATTCAAG 
     1741 GCCTGCGTGG CCTGACACGC CTGCGGCTGG CTGGCAACAC CCGGATTGCC CAGATCCGGC 
     1801 CCGAGGACCT CGCTGGCCTG ACTGCCCTAC AGGAACTGGA TGTGAGCAAC CTGAGCCTGC 
     1861 AGGCCCTGCC CAGTGACCTC TCCAGTCTCT TTCCCCGCCT GCGCCTCCTA GCAGCTGCCC 
     1921 GAAACCCCTT TAACTGCTTA TGCCCCTTGA GCTGGTTTGG TCCTTGGGTT CGTGAGAGCC 
     1981 ATGTTGTGCT GGCCAGCCCT GAGGAGACAC GTTGTCACTT CCCACCCAAG AACGCCGGCC 
     2041 GACTGCTCCT GGAGCTGGAT TATGCAGATT TTGGCTGCCC AGTCACCACT ACCACAGCCA 
     2101 CAGTTCCTAC TATAAGGCCT ACTGTCAGGG AGCCCACACC TTCAACTTCC AGCCAAGCTC 
     2161 CCACCTGGCC CAGCCCCACA GAGCCAACTA CCCAGGCCCC CATCGTACTG TCCACTGCCC 
     2221 CACCAACCAT GAGGCCGGCT CCTCAGCCCC AGGACTGTCC AGCATCCATC TGCCTGAATG 
     2281 GTGGTAGCTG CCGTGTAGGG GCAAAACACC ACCTGGAGTG CCTGTGCCCC GAGGGCTTCA 
     2341 TTGGCCTGTA CTGTGAGAGT CCCGTGGAAC AAAGGACAAA GCCCAGCTCC ATACCGGACA 
     2401 CCCCACGGCC CCCGCGGCTG CTGCCTCTGC GCATTGAGCC GGTGAGCCCC ACCTCCCTGC 
     2461 GTGTGGAGCT GCAGCGCTAC CTGCAGGGCA ACACCGTGCA GCTGCGGAGC CTCCGGCTCA 
     2521 CCTACCGCAA CCTGTCTGGC CCTGACAAGC GGCTGGTGAC GCTGCGGCTG CCTGCTTCAC 
     2581 TTGCAGAGTA CACAGTCACC CAGCTGCGGC CCAATGCCAC CTATTCTATC TGTGTCACAG 
     2641 CCCTGGGAGC TGGGCGGACA CCTGAAGGTG AGGAGGCCTG TGGGGAGGCC AACACTCCCC 
     2701 AGGCCGTCCG CTCCAACCAT GCCCCAGTCA CCCAGGCCCG GGAGGGCAAC CTGCCACTCC 
     2761 TCATTGCACC CGCCCTGGCT GCTGTGCTTC TGGCTGTGTT GGCTGCCTCG GGGGCAGTCT 
     2821 ACTGTGTGCG ACGGGCGCGG GCAAGTTCCA CAGCTCAGGA CAAAGGGCAG GTGGGACCAG 
     2881 GGACCGGGCC CCTGGAACTA GAGGGGGTGA AAGTCCCCTT GGAGCCAGGC TCCAAGGCAT 
     2941 CAGAGGGAGG CGGGGAGGCC CTATCAGGTG GTCCTGAATG TGAGGTGCCC CTCATGGGCT 
     3001 ACCCAGGGCC CAGTCTTCAG GGGGTCCTCC CTGCTCAGCC CTACATTACG CGTACGCGGC 
     3061 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     3121 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     3181 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     3241 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     3301 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     3361 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     3421 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     3481 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     3541 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     3601 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     3661 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     3721 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     3781 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     3841 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     3901 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     3961 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     4021 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     4081 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     4141 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     4201 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     4261 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     4321 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     4381 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     4441 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     4501 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     4561 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     4621 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     4681 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     4741 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     4801 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     4861 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     4921 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     4981 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     5041 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     5101 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     5161 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     5221 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     5281 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     5341 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     5401 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     5461 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     5521 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     5581 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     5641 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     5701 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     5761 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     5821 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     5881 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     5941 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     6001 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     6061 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     6121 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     6181 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     6241 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     6301 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     6361 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     6421 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     6481 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     6541 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     6601 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     6661 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     6721 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     6781 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     6841 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//