LOCUS       RR202803                7809 bp    DNA     circular  SYN 
DEFINITION  Pam (Myc-DDK-tagged ORF) - Rat peptidylglycine alpha-amidating monooxygenase (Pam), (10 ug)
ACCESSION   NM_013000
VERSION     NM_013000.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3966..4057 
                     /label="Myc-DDK"  
     misc_feature    4087..4672 
                     /label="PolyA signal"  
     misc_feature    4144..4163 
                     /label="Restriction Enzyme"  
     misc_feature    4948..5620 
                     /label="ColE1"  
     misc_feature    6050..6844 
                     /label="Kan/Neo"  
     misc_feature    6879..7181 
                     /label="SV40 ori"  
     misc_feature    7267..7744 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3948..3954 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3948 
                     /label="Pam"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCGGACGC GCCCGCAGCG GTCTGCTACT GCTGCTGCTG GGGCTGCTCG 
     1081 CCCTGCAGAG CAGCTGCCTG GCCTTCAGAA GCCCACTTTC TGTCTTTAAG AGGTTTAAAG 
     1141 AAACTACCAG ATCATTTTCC AATGAATGCC TTGGTACCAT TGGACCAGTC ACCCCTCTTG 
     1201 ATGCATCAGA TTTTGCGCTG GATATTCGCA TGCCTGGGGT TACACCTAAA GAGTCTGACA 
     1261 CATACTTCTG CATGTCCATG CGTCTGCCTG TGGATGAGGA AGCCTTCGTG ATTGACTTCA 
     1321 AGCCTCGTGC CAGCATGGAT ACTGTCCACC ATATGCTGCT GTTTGGATGC AATATGCCCT 
     1381 CGTCCACTGG AAGTTACTGG TTTTGTGATG AAGGAACCTG TACAGATAAA GCCAATATTC 
     1441 TATATGCCTG GGCAAGGAAT GCTCCCCCCA CCCGGCTCCC GAAAGGTGTT GGATTCAGAG 
     1501 TTGGAGGAGA AACTGGAAGC AAATACTTCG TCCTTCAAGT TCACTATGGC GATATCAGTG 
     1561 CTTTTCGAGA TAATCACAAA GACTGCTCTG GCGTGTCCGT ACATCTCACA CGTGTGCCCC 
     1621 AGCCTTTAAT TGCGGGCATG TACCTTATGA TGTCTGTTGA CACTGTCATA CCACCAGGAG 
     1681 AGAAAGTAGT GAATGCTGAC ATTTCGTGCC AATACAAAAT GTATCCAATG CATGTGTTTG 
     1741 CCTACAGAGT CCACACTCAC CATTTAGGTA AGGTGGTGAG CGGATACAGA GTAAGAAACG 
     1801 GACAGTGGAC ACTGATTGGA CGCCAGAACC CCCAGCTGCC ACAGGCTTTC TACCCTGTGG 
     1861 AACACCCCGT TGATGTTACT TTTGGTGATA TACTGGCAGC CAGATGTGTG TTCACTGGTG 
     1921 AAGGGAGGAC AGAGGCCACC CATATCGGCG GCACTTCTAG TGACGAAATG TGTAACCTGT 
     1981 ACATCATGTA TTACATGGAA GCCAAATATG CACTTTCCTT CATGACCTGT ACAAAGAACG 
     2041 TGGCTCCAGA TATGTTCAGA ACTATCCCAG CAGAGGCCAA TATCCCAATT CCTGTCAAAC 
     2101 CGGACATGGT TATGATGCAC GGGCATCACA AAGAAGCAGA AAACAAAGAA AAGAGTGCTT 
     2161 TAATGCAGCA GCCAAAACAG GGAGAGGAAG AAGTATTAGA GCAGGGTGAT TTCTATTCAC 
     2221 TGCTTTCCAA GCTGCTAGGA GAAAGGGAAG ATGTTCATGT GCACAAGTAT AATCCTACAG 
     2281 AAAAGACAGA ATCTGGGTCA GACCTGGTAG CTGAGATTGC AAACGTGGTC CAGAAAAAGG 
     2341 ACCTTGGTCG GTCTGACGCC AGAGAAGGTG CAGAGCATGA GGAATGGGGT AATGCTATCC 
     2401 TAGTCAGAGA CAGGATCCAC AGATTCCACC AGCTAGAGTC AACTCTGAGG CCAGCTGAGA 
     2461 GCAGAGCTTT CTCGTTCCAG CAGCCTGGCG AAGGCCCTTG GGAACCAGAA CCCTCAGGAG 
     2521 ATTTCCATGT GGAAGAAGAA CTGGACTGGC CTGGAGTGTA CTTGTTACCA GGCCAGGTTT 
     2581 CTGGGGTGGC CCTGGATTCT AAGAATAACC TAGTGATTTT CCACAGAGGT GACCATGTTT 
     2641 GGGATGGAAA CTCTTTTGAC AGCAAGTTTG TTTACCAGCA AAGAGGTCTT GGGCCAATTG 
     2701 AAGAAGACAC CATCCTGGTC ATTGACCCAA ATAATGCTGA AATCCTCCAG TCCAGTGGCA 
     2761 AGAACCTGTT TTATTTACCA CACGGCTTGA GCATAGATAC AGATGGAAAT TATTGGGTCA 
     2821 CAGATGTGGC TCTCCACCAG GTGTTCAAAT TGGACCCGCA TAGCAAAGAA GGCCCTCTCT 
     2881 TAATTCTGGG AAGGAGCATG CAACCTGGGA GTGACCAAAA TCATTTCTGC CAGCCCACCG 
     2941 ATGTGGCTGT GGAGCCCAGT ACTGGAGCTG TCTTCGTGTC AGACGGTTAC TGTAACAGTC 
     3001 GGATTGTGCA GTTTTCACCA AGCGGAAAGT TCGTCACCCA GTGGGGAGAA GAGTCCTCTG 
     3061 GAAGCAGTCC TAGGCCAGGC CAGTTCAGTG TTCCTCACAG TTTGGCCCTT GTGCCTCATT 
     3121 TGGACCAGTT GTGTGTGGCA GACAGGGAAA ATGGCCGAAT CCAATGCTTC AAAACTGACA 
     3181 CCAAAGAATT TGTGAGAGAG ATTAAGCACG CATCATTTGG AAGGAATGTC TTTGCCATTT 
     3241 CATATATACC AGGTTTCCTC TTTGCCGTAA ACGGGAAGCC TTACTTTGGA GACCAAGAGC 
     3301 CCGTGCAAGG ATTTGTGATG AACTTTTCCA GTGGGGAAAT TATAGACGTC TTCAAGCCAG 
     3361 TACGCAAGCA CTTCGACATG CCTCATGATA TTGTGGCTTC TGAAGATGGG ACTGTGTACA 
     3421 TTGGAGACGC ACACACAAAC ACCGTGTGGA AGTTCACCCT GACTGAAAAA ATGGAGCATC 
     3481 GGTCAGTTAA AAAGGCTGGC ATTGAAGTCC AGGAAATCAA AGAAGCCGAG GCAGTTGTTG 
     3541 AACCCAAAGT GGAGAACAAA CCCACCTCCT CAGAATTGCA GAAGATGCAA GAGAAACAGA 
     3601 AACTGAGCAC AGAGCCCGGC TCGGGAGTGT CCGTGGTTCT CATTACAACC CTTCTGGTTA 
     3661 TTCCTGTGCT GGTCCTGCTG GCCATTGTCA TGTTTATTCG GTGGAAAAAA TCAAGGGCCT 
     3721 TTGGAGATCA TGACCGCAAG CTCGAGTCAA GTTCTGGAAG AGTCCTGGGA AGATTCCGAG 
     3781 GAAAGGGAAG CGGCGGCTTA AATCTGGGAA ATTTCTTTGC AAGTCGAAAA GGCTACAGCA 
     3841 GAAAAGGGTT TGACCGAGTG AGCACAGAGG GGAGTGACCA AGAGAAAGAT GAGGACGACG 
     3901 GAACTGAGTC TGAAGAGGAG TACTCGGCCC CGCTGCCCAA GCCTGCACCT TCCTCCACGC 
     3961 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4021 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4081 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4141 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4201 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4261 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4321 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4381 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4441 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4501 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     4561 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     4621 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     4681 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     4741 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     4801 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     4861 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     4921 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     4981 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5041 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5101 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5161 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5221 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5281 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     5341 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     5401 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     5461 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     5521 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     5581 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     5641 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     5701 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     5761 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     5821 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     5881 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     5941 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6001 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6061 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6121 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6181 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6241 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6301 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     6361 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     6421 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     6481 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     6541 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     6601 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     6661 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     6721 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     6781 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     6841 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     6901 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     6961 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7021 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7081 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7141 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7201 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7261 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7321 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     7381 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     7441 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     7501 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     7561 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     7621 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     7681 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     7741 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     7801 GCGAATTTT
//